• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.11 no.3
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• pp.277-282
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• 2018

The goal of the secret message communication on the internet is to maintain invisibility and confidentiality. Digital steganography is a technique in which a secret message is inserted in a cover medium and transmitted to a destination so that a third party can not perceive the existence of the message itself. Steganography is an efficient method for ensuring confidentiality and integrity together with encryption techniques. In order to insert a secret (Hangul) message, I propose a image steganography method that the secret character is separated and converted into binary code with reference to the encryption table, the cover image is divided into two areas, and the secret message and the right l-LSB information of the second area are encrypted and crossed, concealing the k-LSB of the first region. The experimental results of the proposed method show that the PSNR value is 52.62 and the acceptable image quality level.

• Korean Journal of Microbiology
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• v.48 no.2
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• pp.141-146
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• 2012

A gene encoding the xylanase (XynA) predicted from partial genomic sequence of Paenibacillus woosongensis was cloned into Escherichia coli by PCR. This xynA gene consisted of 633 nucleotides, encoding a polypeptide of 211 amino acid residues. The deduced amino acid sequence exhibited 85-89% identity with those of several Paenibacillus xylanases, belonging to the glycosyl hydrolase family 11. As a results of expression of the structural gene by T7 promoter of a pET23a(+) expression vector, xylanase activity was higher in cell-free extract than culture filtrate of a recombinant Escherichia coli BL21(DE3) CodonPlus. However, the expression level of xylanase was not sufficient be detected by SDS-PAGE. The cell-free extract showed maximal xylanase activity at $60^{\circ}C$ and pH 5.5. The predominant products resulting from xylan and xylooligosaccharide hydrolysis were xylose and xylotriose. The enzyme could hydrolyze xylooligosaccharides larger than xylbiose.

• KSBB Journal
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• v.25 no.2
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• pp.167-172
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• 2010

Streptomyces scabiei producing phytotoxin called thaxtomin, which cause scab disease on economically important crops such as potato. For molecular genetics study of S. scabiei an effective transformation method was established based on conjugal transfer from Escherichia coli ET12567 (pUZ8002) using a phiC31-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 50 mM $MgCl_2$. In addition, the sequence and location of the chromosomal integration attB site of S. scabiei was identified for the first time in the strains producing thaxtomin by the southern blot analysis of exconjugants and the sequencing of plasmid containing DNA flanking the insertion sites from exconjugant chromosome. Similar to the case of Streptomyces species, a single phiC31 attB site of S. scabiei is present within an ORF encoding a pirin-homolog.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.45 no.1
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• pp.115-119
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• 2008

In this paper, the linearity of Doherty power amplifier has been improved by applying a new Photonic Bandgap(PBG) structure on the output of amplifier. The reposed spiral PBG structure is a two-dimensional(2-D) periodic lattice patterned on a dielectric slab that does not require nonplanar fabrication process. This structure has more broad stopband and high suppression performance than the conventional three cell PBG. Also, It has a sharp skirt property. We obtained the 3rd-order intermodulation distortion(IMD3) of -33dBc for CDMA applications with that of maintaining the constant power added efficiency(PAE), the IMD3 performance is improved as much as -8 dB compared with a Doherty power amplifier without PBG structure. Moreover, the physical length of PBG is shortened, therefore the whole amplifier circuit size is considerably reduced.

• Journal of Life Science
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• v.20 no.1
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• pp.97-102
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• 2010

Streptomyces platensis YK-2, newly isolated from forest soil, produces transglutaminase (TGase), which catalyses an acyl transfer reaction between the primary grade amine and protein or $\gamma$-carboxyamide group of peptide bound glutamine residues. For a molecular genetic study of S. platensis, an effective transformation method was established by using a conjugal transfer of DNA from Escherichia coli to spores of actinomycetes. The highest transconjugation frequency of S. platensis was obtained on an MS medium containing 50 mM $MgCl_2$, using $5{\times}10^7\;E$. coli as a DNA donor and $1{\times}10^8$ spores without heat treatment as a host. We also identified that S. platensis contains a single attB site within an ORF encoding a pirin-homolog, and that its attB site sequence shows high homology to that of S. logisporoflavus. In addition, it was confirmed by phenotypic analyses of exconjugants that the introduction of heterologous DNA into the attB site of the S. platensis chromosome does not affect its morphological differentiation and TGase production.

• Journal of the Korean Institute of Intelligent Systems
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• v.10 no.3
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• pp.252-259
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• 2000

In this paper, we propose a Fuzzy Classifier System(FCS) to find a set of fuzzy rules which can carry out the edge detection of a image. The FCS is based on the fuzzy logic system combined with machine learning. Therefore the antecedent and consequent of a classifier in FCS are the same as those of a fuzzy rule. There are two different approaches, Michigan and Pittsburgh approaches, to acquire appropriate fuzzy rules by evolutionary computation. In this paper, we use the Michigan style in which a single fuzzy if-then rule is coded as an individual. Also the FCS employs the Genetic Algorithms to generate new rules and modify rules when performance of the system needs to be improved. The proposed method is evaluated by applying it to the edge detection of a gray-level image that is a pre-processing step of the computer vision. the differences of average gray-level of the each vertical/horizontal arrays of neighborhood pixels are represented into fuzzy sets, and then the center pixel is decided whether it is edge pixel or not using fuzzy if-then rules. We compare the resulting image with a conventional edge image obtained by the other edge detection method such as Sobel edge detection.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.44 no.7 s.361
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• pp.29-34
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• 2007

This paper presents a novel photonic bandgap (PBG) structure for size reduction and linearity improvement in power amplifier. The proposed structure is a two-dimensional (2-D) periodic lattice patterned on a dielectric slab that does not require nonplanar fabrication process. Throughout the experi-mental results, this structure has more broad stopband and high suppression performance than conventional three cell PBG and distorted uniplanar compact-PBG (DUC-PBG). This new PBG structure can be applied with power amplifier for linearity improvement. The 3rd intermodulation distortion (IMD3) of the power amplifier using new PBG structure is -36.16 dBc for (code division multiple access) CDMA applications. Compared with power amplifier without the proposed PBG structure, improved IMD3 is -13.49 dBc.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.5
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• pp.435-441
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• 2015

In this study, a method for optimal design of impeller and diffuser blades in the centrifugal compressor using response surface method (RSM) and multi-objective genetic algorithm (MOGA) was evaluated. A numerical simulation was conducted using ANSYS CFX with various values of impeller and diffuser parameters, which consist of leading edge (LE) angle, trailing edge (TE) angle, and blade thickness. Each of the parameters was divided into three levels. A total of 45 design points were planned using central composite design (CCD), which is one of the design of experiment (DOE) techniques. Response surfaces that were generated on the basis of the results of DOE were used to determine the optimal shape of impeller and diffuser blade. The entire process of optimization was conducted using ANSYS Design Xplorer (DX). Through the optimization, isentropic efficiency and pressure recovery coefficient, which are the main performance parameters of the centrifugal compressor, were increased by 0.3 and 5, respectively.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.45 no.9
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• pp.49-54
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• 2008

In this paper, the power added efficiency(PAE) of class F power amplifier is improved by applying a new Photonic Bandgap (PBG) structure on the output of amplifier. The proposed spiral PBG structure is a two-dimensional (2-D) periodic lattice patterned on a dielectric slab that does not require nonplanar fabrication process. This structure bas higher suppression performance at second harmonic. Also, It has a sharp skirt property. This new PBG structure can be applied with class F power amplifier for efficiency improvement. We obtained the PAE of 73.62 % for CDMA applications, and the PAE performance is improved as much as 6.2 % compared with that of a conventional class F power amplifier.

• Korean Journal of Microbiology
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• v.38 no.3
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• pp.173-179
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• 2002

The functions of riboflavin synthesis genes ( ribI,II,III and IV) found immediately downstream of luxG in the lux operon from Photobacterium species were identified using the biochemical and genetical analysis. The ribI-III gene codes for protein corresponding to that coded by the second (riboflavin synthase), third (3,4-dihydroxy 2-butanone 4-phosphate synthase/GTP cyclohydrolase II) and fourth (lumazine synthase) gene, respectively, of Bacillus subtilis rib operon with the respective gene procuct sharing 41-50% amino acid sequence identity. Unexpectedly, the sequence of the ribIV product of Photobacterium phosphoreum does not correspond in sequence to the protein encoded by the fifth rib gene of Bacillus subtilis. Instead the gene (ribIV) codes for a polypeptide similar in sequence to GTP cyclohydrolase II of Escherichia coli and the carboxy terminal domain of the third rib gene from Bacillus subtilis. Complementation of Escherichia coli riboflavin auxotrophs showed that the function of the gene products of ribII and ribIV are DHBP synthase and GTP cyclohydrolase II, respectively. In addition the experiment, showing that increase in thermal stability of riboflavin synthase coded by ribIon coexpression with ribIII, provided indirect evidence that the latter gene codes for lumazine synthase.