• Title/Summary/Keyword: 유전변이

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Genetic Variation of some Patches of Eleutherococcus senticosus (Rupr. & Maxim) Maxim. in Korea (국내(國內) 가시오갈피 군락(群落)의 유전변이(遺傳變異) 분석(分析))

  • Hong, Kyung-Nak;Cho, Kyung-Jin;Park, Yew-Heon;Hur, Sung-Du;Hong, Yong-Pyo;Kang, Bum-Yong
    • Journal of Korean Society of Forest Science
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    • v.89 no.5
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    • pp.645-654
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    • 2000
  • The aim of this study was to described the genetic structure of Eleutherococcus senticosus in Korea. We investigated 10 patches, which are eight Korean patches and two foreign patches come from Russia and China growing at Korean habitat, using ISSR(inter-simple sequence repeats) markers. In ISSR PCR, the overall percentage of polymorphic ISSR amplicons was 76% and the mean number of amplicons per ISSR primer was 11.5, which were higher than the RAPD results for the some cultivars collected in Korea(Kim et al., 1998) ; 57% and 5.7, respectively. So ISSR markers provide more powerful tool than RAPD markers for the investigation of genetic variation in E. senticosus. There are relatively high genetic variation among patches as 62.8%, but low variation within eight Korean patches. Such pattern of genetic variation, which is not ordinary in other tree species, may be result from the narrow and limited habitats and the asexual reproduction of this species at the natural stands in Korea. Although the small sample size in this study seemed to be resulted in the high genetic variation among patches, the overall genetic interpretation of this study might not be much affected on the basis of the characteristics of the distribution and the reproduction system of E. senticosus. Analysis of genetic distance between all pairs of the patches did not reveal any trends with regard to geographic distance, which was confirmed by the results obtained from AMOVA(analysis of molecular variance) and PCA(principal component analysis). These results suggest that, in addition to the preservation of the natural stands, the conservation of larger number of patches with small number of individuals per patch is more effective for the ex situ conservation and for maintaining the genetic diversity of E. senticosus in Korea than smaller patches with large number of individuals.

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Genetic Variation and Population Structure of the Slender Bitterling Acheilognathus lanceolatus of Korea and Japan as Assessed by Amplified Fragment Length Polymorphism (AFLP) Analysis (AFLP 분석에 의한 한국과 일본의 납자루 Acheilognathus lanceolatus의 유전 변이와 집단 구조)

  • Yun, Young-Eun;Kim, Chi-Hong;Kim, Keun-Yong;Ishinabe, Toshihiro;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.22 no.2
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    • pp.115-120
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    • 2010
  • Genetic variation and population structure of the slender bitterling Acheilognathus lanceolatus of Korea (the Han, Geum, Dongjin, Seomjin and Nakdong Rivers) and Japan (the Katsura River) were assessed by amplified fragment length polymorphism (AFLP) analysis. Five combinations of selective primers generated 345~374 DNA fragments, of which 55~131 were polymorphic. The Nakdong River population had the highest genetic diversity and the Han River population had the lowest genetic diversity. Dendrogram based on the distance matrix revealed that individuals from each population consistently clustered together and bifurcated into two distinct clades (or population groups) composed of the Han, Geum, Dongjin and Seomjin River populations and of the Nakdong and Katsura River populations, supported with high bootstrap values. The pairwise genetic differentiation ($F_{ST}$) estimates showed that the six populations were genetically well differentiated (P<0.01). The analysis of molecular variance (AMOVA) after partitioning the six populations into two population groups revealed very strong biogeographic structuring between them with 25.49% of total variance (P<0.01). Taken together, the AFLP markers clearly divided six A. lanceolatus populations into two population groups.

Studies on the Community Structure, Samara and Leaf Shape of Three Natural Acer pictum subsp. mono Forest (고로쇠나무 천연림의 지역별 군집구조, 시과 및 엽 형태)

  • Kim, Gab Tae;Kim, Hoi Jin;Lee, Jae Hyeun
    • Korean Journal of Environment and Ecology
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    • v.28 no.1
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    • pp.55-61
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    • 2014
  • To examine the forest structure and variation of samara and leaf shape of Acer pictum subsp. mono (APSM), We investigated forest structure and samara angles and length, and leaf shape in three natural population of APSM in Yanggu-gun, Youngyang-gun and Kwangyang-si from June 2012 to October 2013. Mean importance percentage (MIP) of APSM is shown lower values in Yanggu 19.8%, in Youngyang 22.0%, and in Kwangyang 17.1%, respectively. In middle layer, importance percentage (IP) of APSM is shown lower values than that of Acer pseudosieboldianum and Carpinus cordata. In lower layer, importance percentage (IP) of APSM is shown lower values in Yanggu 1.8%, in Youngyang 1.9%, and in Kwangyang 0.0%, respectively. From these results, MIP of APSM in natural population might be reduced in future. Angles between wings and samara length are significantly different between districts. APSM with seven palmate vein leaf are distributed more frequently at Kwangyang and Youngyang than Yanggu. Percentages of biserrate leaf is shown in Yanggu 0.6%, in Youngyang 15.8%, and in Kwangyang 20.4%, respectively. These results implies genetic variation in natural APSM population and further studies on the genetic variation analysis in natuel population of APSM should be needed.

Evaluation of Crop Characteristics of Sorghum (Sorghum bicolor L.) Germplasm for the Selection of Excellent Resources (우수자원 선발을 위한 수수(Sorghum bicolor L.) 유전자원의 특성평가)

  • Yoon, Seong-Tak;Jeong, In-Ho;Han, Tae-Kyu;Kim, Young-Jung;Yu, Je-Bin;Yang, Gyeong;Ye, Min-Hee;Baek, Seung-Woo;Kim, Kun-Woo
    • Korean Journal of Plant Resources
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    • v.29 no.4
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    • pp.479-494
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    • 2016
  • The aim of this study is to select the superior resources of high yield, high content of functional material optimal to mechanical harvesting by the evaluation of crop growth and yield characteristics in sorghum germplasm. One hundred accessions of sorghum germplasm were used in this experiment. Days from seeding to heading date showed the range from 68 to 94 days with the highest frequency proportion was the group from 80 to 85 days, which occupied 34% (34 plant resources) of 100 germplasm. Ear types of 100 sorghum germplasm could be classified as 7 types of broom-tillering, half broom-tillering, extreme open-loose type, open-loose type, intermediate type, compact type, extreme-compact type of which intermediate type was the highest ratio of 28% (28 plant resources) of 100 germplasm. Yield showed the range from 106 to 365 ㎏/10a with the highest frequency proportion of it was the group from 150 to 200 ㎏/10a, which occupied 44% (44 plant resources) of 100 germplasm. Among 100 sorghum germplasm, 18 ideal resources of high yield and short plant height appropriate for mechanical harvesting were selected. In order to evaluate high content of functional substance, selected 18 resources were analyzed for total polyphenol content, DPPH radical scavenging activity and total anthocyanin content. Finally, we selected 5 resources of short plant height, high yield, high content of total polyphenol and high DPPH radical scavenging activity among 18 genetic resources.

Seed Protein Content and Fatty Acid Composition of Black Seeded Soybeans Collected From Southwestern Islands (서남해안 검정콩들의 단백질과 지방산 조성변이)

  • 권병선;신정식
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.3
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    • pp.240-243
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    • 2002
  • A total of 135 black seeded soybeans genotypes were collected in 45 island locations from January to May 2001. Seeds of 135 genotypes collected were analyzed for crude protein and fatty acid compositions. The crude protein content was averaged to be 40.75%, and was ranged from 34.70% to 44.20%. The average palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid content were 11.41%, 3.93%, 22.75%, 53.55%, and 8.35%, and the ranges of those were 9.00% to 14.40%, 2.90% to 5.00%, 22.75% to 26.50%, 50.30% to 57.20% and 6.7% to 11.20%, respectively. Heritabilities of palmitic acid, oleic acid, linoleic acid, and crude protein were higher, but that of stearic acid and linolenic acid were relatively lower, Crude protein content was correlated positively with oleic acid content, whereas it was correlated negatively with linoleic acid, palmitic acid, stearic acid, and linolenic acid contents.

Mitochondrial DNA Variations among Three Species of Korean Planorbid Snails : Gyraulus convexiusculus, Hippeutis cantori and Segmentina hemisphaerula (한국산 또아리물탈행이과 3종 내의 미토콘드리아 DNA 변이)

  • Chung, Pyung-Rim;Younghun Jung;Jung, Eung-Kyung;Kim, Dae-Soon
    • The Korean Journal of Malacology
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    • v.10 no.1
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    • pp.27-37
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    • 1994
  • 한국산 담수 또아리물달팽이과(Planorbidae)에 속하는 또아리물달팽이(Gyraulus convexiusculus),수정또아리물달팽이(Hippeutis cantori) 및 배꼽또아리물달팽이(Segmentina hemisphaerula)3종에 대한 종간 유전적 변이와 이들 상호간의 분류학적 유연 관계를 생화학적 측면에서 밝히고자 하였다. 즉, 모계유전으로 자손에 유전되고 있는 미토콘드리아 DNA(mitochondrial DNA; mt DNA)의 변이를 보기위하여 제한효소(restriction enzyme)를 처리하고 잘라진 mtDNA절편들을 상호 비교하는 restriction fragmint length polymorphism(RFLP)기법을 응용하였다. 본 실험에서 10개의 제한효소 중 CIa I, Dra I, Eco RI, Hin dIII, Kpn I및 pst I의 6개 제한효소에서 좋은 결과를 얻어 종간의 공통절편(shated fragmints)을 비교하였고, 염기분화율(nucleotide divergince rate)을 각각 측정하였다. 미토콘드리아 DNA 크기(genome size)는 또아리물달팽이가 12.08 kb, 수정또아리물달팽이가 14.4 kb, 그리고 배꼽또아리물달팽이가 12.93 kb로 관찰되었다. 염기분화율(p)는 또아리물달팽이/수정또아리물달팽이 군에서 p=12.7%, 배꼽또아리물달팽이와 상기 2종군 사이의 염기분화율은 P=56.6%여서 배꼽 또아리물달팽이류는 타 2종보다 그 분화율이 매우 높음을 알 수 있었다. 이상의 결과로 보아 분류군(taxa)의 mtDNA 변이에 의한 RELP기법이 앞으로 한국산 담수 패류 연구에 널리 응용될 수 있음이 확인되었다.

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Design of Metadata for Provenance Management of Genome Data (유전체 데이터의 유래(Provenance) 관리를 위한 메타데이터의 설계)

  • Song, Myoung-Seon;Chang, Jae-Woo;Um, Jung-Ho;Choi, Dong-Hoon
    • Proceedings of the Korea Information Processing Society Conference
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    • 2011.11a
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    • pp.1195-1198
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    • 2011
  • 최근 의료 분야에 대한 관심이 높아짐에 따라 유전체 데이터를 수집하고 관리하여 분석하는 기술에 대한 많은 연구가 수행되고 있다. 유전체 데이터는 크게 유전체 데이터를 분석하는 전처리단계와 유전체 데이터로부터 변이된 유전체 데이터를 생성하는 후처리단계를 통해 분석된다. 이러한 분석 과정은 많은 시간이 소요되며, 후처리단계에서 결과 데이터는 분석 알고리즘 및 처리 기법에 따라 상이한 결과 데이터를 생성한다. 또한, 유전체 데이터의 각 파이프라인 별 분석된 데이터의 관리가 필요하다. 본 논문에서는 유전체 데이터의 특성을 고려하여, 유전체 데이터 유래 관리를 위한 메타데이터를 설계한다. 아울러 데이터 유래 메타데이터는 자신의 이전데이터들의 결과데이터에 신속한 접근이 가능해야하며, 자신과 유사한 데이터 유래를 지닌 파이프라인의 상세 정보를 신속하게 검색하는 색인구조가 필요하다. 따라서 이를 고려한 유래 메타데이터 검색 알고리즘을 설계한다.