• Title/Summary/Keyword: 유전다양성

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넙치(Paralichthys olivaceus) 정액의 냉동보존과 보존정자를 이용한 치어 생산

  • 장윤정;장영진;이정용
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.10a
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    • pp.283-284
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    • 2001
  • 넙치(Paralichthys olivaceus)는 1986년부터 본격적으로 양식되기 시작한 어종으로 현재 국내 어류 양식에서 차지하는 비율이 가장 높다. 그러나, 한정된 친어에서 생산된 수정란의 사용으로 유전적 다양성이 감소하고 있어, 우량한 형질의 유전자를 가진 친어를 이용하여 유전적 다양성을 유지하는 일이 필요하다. 또한, 일본에서 수입된 수정란으로 생산된 치어가 우리나라 연안에 방류됨으로써, 자연 상태에서 국산 넙치와의 잡종이 발생하고 있어 국산 넙치의 종보존이 시급한 실정이다. (중략)

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Permittivity Measurements of various Materials in 13-28GHz Band (13-28GHz 대역에서 다양한 매질의 유전율 측정)

  • Park, No-Uk;Jeong, Won-Ho;Jung, Myung-Won;Chong, Young-Jun;Kim, Jong-Ho;Kim, Kyung-Seok
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.14 no.1
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    • pp.161-167
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    • 2014
  • This paper presents the results of permittivities of various materials by measurements of the dielectric properties for the materials in millimeter-wave band. Since the advantages of millimeter wave band include the miniaturization, weight reduction of component and a lot of information transfer, its study has received increasing attention of researches. Also, because of the characteristics of the band have the superior straightness and the small diffractive, in the band, the analysis for the reflection and transmission characteristics of the materials will be very important. Moreover, a low interference is one of characteristics of the band due to the fact that the millimeter wave is absorbed in the materials. Therefore, in this paper, the reflection and transmission characteristics of materials are measured in the band by using the free space method. Based on the presented result, the permittivities of the materials are derived by the calculation of Nicolson-Ross Weir method.

Genotoxicity Study of Immature Green Persimmon Extract (풋감 주정 추출물의 유전독성 연구)

  • Ham, Young-Min;Yoon, Seon-A;Hyun, Ho Bong;Go, Boram;Jung, Yong-Hwan;Oh, Dae-Ju;Yoon, Weon-Jong
    • Journal of Food Hygiene and Safety
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    • v.35 no.6
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    • pp.567-573
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    • 2020
  • The persimmon is commonly cultivated in temperate regions of the world, including China, Korea, Japan, Brazil, Turkey, and Italy. In some Asian cultures, consumers are aware of the health claims related to the persimmon and its functional ingredients. The rich phytochemistry of the persimmon has opened new avenues of research on diet-based regimens designed to cure various ailments. This study was conducted to identify the genotoxicity of immature green persimmon (Diospyros kaki THUNB.) extract (DKA). The bacterial reverse mutation assay, the chromosomal aberration assay, and the mammalian micronucleus test were performed to determine the DKA genotoxicity. The result of the bacterial reverse mutation assay revealed that the DKA did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia coli WP2uvrA with or without metabolic activation of S9 mixture. The oral administration of DKA also caused no significant increase in the number of micronucleated polychromatic erythrocytes or in the mean ratio of polychromatic to total erythrocytes. In addition, DKA did not cause a significant chromosome aberration on CHL cells in the presence or absence of S9 activation. In conclusion, DKA could be considered as a reliable and safe functional food since no toxicity was found under the condition of this study.

Diversity and Inheritance of AFLP Markers in Wild and Cultivated Soybeans (AFLP marker를 이용한 콩의 유전적 다양성과 유전분리 분석)

  • 김용호;윤홍태
    • Korean Journal of Plant Resources
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    • v.17 no.3
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    • pp.265-271
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    • 2004
  • Genetic variation is the basis of crop improvement. Limited genetic diversity in a crop species may restrict the amount of genetic improvement that can be achieved through plant breeding. Soybean is one of the world's most important crops. A potential source of genetic variability for the cultivated soybean is the wild species G. soja Sieb. & Zucc. Amplified fragment length polymorphism (AFLP) analysis is a PCR-based technique, which can detect a 10-fold greater nubmer of loci than other DNA marker analysis. Twenty cultivated soybeans and two-hundred wild soybeans were used to determine genetic vatiations by AFLPs and evaluate the usefulness of AFLPs as DNA markers. Six-hundred and ten fragments were detected with an average of 56 AFLP fragments produced per primer in a total of 11 AFLP primer pairs. The number of polymorphic loci detected per primer ranged from 7 to 20 and the polymorphism was greater in wild than in cultivated soybean. F$_2$ segregation analysis of four AFLP fragments in combination of Hwaeomputkong ${\times}$ PI 417479 indicated that they segregate as stable Mendelian loci with 3 : 1. This results strongly suggest that the AFLP analysis is a good technique for the detection of genetic polymorphism in a wide plant species.

The Potential of the Korean Endemic Plant, Abeliophyllum distichum, as a Plant Resource (한국 특산 식물 미선나무, 식물자원으로의 잠재력)

  • Tae Won Jang
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2023.04a
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    • pp.9-9
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    • 2023
  • 한국 특산 식물인 미선나무 (Abeliophyllum distichum)는 IUCN Red List의 EN (위기종) 등급에 지정되 어 보호받아 왔다. 미선나무는 국내 자생지 6곳을 포함하는 13개의 서식지에서 생육되고 있다고 알려져 있다. 2017년 이후, 국내에서는 지속적인 보전과 관리하에 법정보호종에서 해제되어 멸종위기종 복원 성 공 사례의 귀감이 되었으나 국내를 제외하면 미선나무의 활용은 아직 어려운 현실이다. 미선나무는 1속 1종이지만 종 내에서 다양성이 존재한다. 꽃의 형태적 특징에 따른 다양한 분류가 이루어지며, 옥황1호을 포함한 분홍, 상아, 연녹색, 흰색 등으로 구분된다. 유전체 연구 및 성분 분석을 통해, 각 표현형마다 유전 정보와 유효 성분의 차이가 존재한다는 것이 알려졌다. 활용 가치를 높이려면, 유전적으로 안정되고 표준화가 가능한 원료가 필요하며, 이는 신품종을 이용하거나 새로운 개발이 필요하다는 것을 내포한다. 일반 식품으로의 활용은 어려우나 기능성 화장품의 원료 등으로 활용되고 있으며, 이러한 활용들은 미선나무의 높은 활용 가치를 투영한다. 활용 가치 평가를 위해 수행된 DNA 손상, 멜라닌 생합성, 항염증, 항산화, 항암 억제 등의 연구를 통해 다양한 약리학적 효능을 가진다는 결과들이 도출되었고, 많은 문헌들이 그 근거를 뒷받침한다. 또한, 유효 성분의 합성경로를 재정립하고 RNA 분석을 통해 그 양상을 예측하기 위한 연구가 진행 중에 있다. 미선나무에서 유래한 식물조직 배양체인 callus의 유도 및 배양이 진행되고 있으며, 옥황1호 유래 callus를 활용하여 다양한 연구를 수행하고 있다. 앞서 언급한 미선나무 연구 및 개발에 대한 한계를 파악하고, 천연 식물 자원으로의 미선나무에 대한 지속적인 관심과 지원을 통해 나고야의정서에 대응하는 전략적 접근이 필요하다.

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Genetic Differentiation between Up- and Downstream Populations of Tribolodon hakonensis (Pieces: Cyprinidae) (삼척오십천 상.하류에 분포하는 황어, Tribolodon hakonensis (잉어과) 집단의 유전적 분화)

  • Lee, Sihn-Ae;Lee, Wan-Ok;Suk, Ho-Young
    • Korean Journal of Environment and Ecology
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    • v.26 no.4
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    • pp.475-483
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    • 2012
  • Tribolodon hakonensis(Cypriniformes; Leuciscinae) is anadromous; they are born in freshwater, migrate back to the ocean, then return to their home stream for spawning from mid-March to early-June. Here, five microsatellites were used to assess the level of gene flow among T. hakonensis populations from the Samcheok-Oship Stream, South Korea. The frequencies of dominant alleles across several loci differed between down-and upstream populations divided by several weirs, and pairwise multilocus $F_{ST}$ estimate was significantly high(0.083). However, there were no signs of any loss of genetic variation in the upstream population. Assignment tests of individuals in admixture model(K=2) to a set of baseline samples showed fairly correct assignment to each cluster; all of upstream individuals sere included in the first cluster, while the majority of downstream individuals(65%) comprise the second cluster. These results indicate reduced gene flow between up- and downstream populations but allowing passive downstream drift. It is likely that man-made structures might at least partially be a factor for creating and consolidating the current distribution patterns of genetic variation among T. hakonensis populations in the Samcheok-Oship Stream. This information will assist governing agencies in making informed decisions regarding conservation of anadromous fishes in Korean drainage systems.

Genetic Algorithm Applying Modified Mutation Operator Based on Hamming Distance for Solving Multi-dimensional Knapsack Problem (개체간 해밍 거리 기반의 변이연산을 적용한 유전알고리즘을 이용한 다차원 배낭 문제 탐색)

  • Jeong, Jae-Hun;Lee, Jong-Hyun;Ahn, Chang-Wook
    • Proceedings of the Korea Information Processing Society Conference
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    • 2012.11a
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    • pp.1728-1731
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    • 2012
  • 본 논문에서는 부모 개체의 해밍 거리에 기반하여 선택적 변이연산을 적용한 유전알고리즘을 제안한다. 유전자 형이 매우 유사한 개체들 간의 유전연산은 알고리즘의 탐색성능을 저하시키고 조기 수렴의 가능성을 증가시킨다. 본 논문에서는 이러한 현상을 극복하기 위하여, 교차연산 시 선택된 두 부모 개체간의 해밍 거리에 따라 그 값이 낮으면 교차연산 후 생성된 두 자식 개체 중 한쪽에게 높은 변이확률을 적용하고 다른 한쪽 자식은 부모와 비슷한 유전자 형으로 탐색을 계속하게 하여 조기 수렴을 방지하면서 해집단의 다양성 유지 기능을 향상 시켰다. 제안한 유전 알고리즘을 다차원 배낭 문제에 적용한 결과, 같은 조건에서 단순 유전 알고리즘(SGA) 보다 향상된 탐색 성능을 보여주었다.

Genetic diversity and geographic differentiation in the endangered Primula farinosa subsp. modesta, a subalpine endemic to Korea (한반도 아고산대 특산·희귀식물 설앵초의 유전적 다양성과 지리적 분화)

  • Chung, Jae-Min;Son, Sung-Won;Kim, Sang-Yong;Park, Gwang-Woo;Kim, Sung-Shik
    • Korean Journal of Plant Taxonomy
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    • v.43 no.3
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    • pp.236-243
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    • 2013
  • Many plant species in subalpine regions are under threat of extinction as a result of climate change. In this study, the genetic diversity and geographic differentiation of three regions and six populations of Primula farinosa subsp. modesta (Bisset & Moore) Pax in Korea were assessed using the ISSR (Inter Simple Sequence Repeat) marker. The average genetic diversity (P = 60.62, SI = 0.299, h = 0.190) was relatively lower than that of other long-lived perennials, even though it is a self-incompatible species. AMOVA analysis showed that 50% of the total genetic diversity was partitioned among regions and Bayesian cluster analysis showed some remarkable geographic trends that were structured into 2 or 3 regions, suggesting limited gene flow among regions. Considering the population fragmentation, low level genetic diversity, and high genetic differentiation, it is essential to establish in situ and ex situ conservation strategies for P. farinosa subsp. modesta.

Genetic Diversity and Population Structure of the Endangered Fish Pseudopungtungia nigra (Cyprinidae) from the Geum and Mankyung Rivers Assessed by Amplified Fragment Length Polymorphism (금강과 만경강에 서식하는 멸종위기 어류 감돌고기 Pseudopungtungia nigra의 AFLP에 의한 유전 다양성 및 집단구조)

  • Kim, Keun-Sik;Yun, Young-Eun;Kang, Eon-Jong;Yang, Sang-Geun;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.21 no.2
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    • pp.76-80
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    • 2009
  • Genetic diversity and genetic structure within the Geum River and Mankyung River populations of the Korean endangered Black shinner (Pseudopungtungia nigra) were assessed by amplified fragment length polymorphism (AFLP). AFLP analysis using five primer combinations generated 447 AFLP bands with 64.1% polymorphism (Geum River 74.6% and Mankyung River 53.6%). The heterozygosities within the two populations were calculated to be 0.170 and 0.104, respectively. Their average genetic diversities are 0.240 and 0.147, respectively. The pairwise Fst value (0.150) indicated distinct genetic differentiation between the two populations. A UPGMA dendrogram based on genetic distance among the individuals revealed a division corresponding to geographical regions, with low genetic variation within the Mankyung River population, and low genetic distance (0.026) between the two populations. Consequently, the two populations may have the same genetic origin The Geum River population will be more suitable than the Mankyung River population for conservation plans to increase the population sizes. Genetic and habitat management will be necessary for the Mankyung River population.