• Korean Journal of Environment and Ecology
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• v.32 no.6
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• pp.566-574
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• 2018

The purpose of this study was to identify the phylogenetic relationships of the plants in the Korean genus Suaeda and to find out the molecular markers that could confirm the interspecies relationships in the family tree through molecular phylogenetic studies. We used the nuclear ribosomal DNA ITS and the chloroplast DNA matK, psbA-trnH, and trnL-trnF as the molecular markers. We could not distinguish between S. japonica and S. maritima and between S. maritima and S. australis in the ITS region and could not distinguish between S. japonica and S. australis with the base sequence in the psbA-trnH and trnL-trnF region. However, we analyzed the combinations of four molecular marker regions and confirmed that each of five plant species of the genus Suaeda formed the independent line. Therefore, it is considered that combinations of molecular markers would be useful for the analysis of phylogenetic relationships in the genus Suaeda. Further investigations of the ecological and morphological characteristics would be needed to understand the phylogenetic relationship and lineage diversification in the genus Suaeda.

• Journal of Animal Science and Technology
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• v.53 no.4
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• pp.303-310
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• 2011

Phylogenetic relationships of Jeju dogs to other domestic and foreign dog breeds were assessed using mtDNA D-loop sequences. Neighbor-joining trees were constructed using complete sequences (970 bp excluding the tandem repeat region) determined for five Cheju, four Jindo, four Sapsaree, five Pungsan, two of each East and West Laika dogs (Canis familiaris), two gray wolves (Canis lupus) and two coyotes (Canis latrans) and also published complete sequences for dogs. Coyote sequences were used as outgroups. In addition, a total of 214 haplotypes of 598bp D-loop sequences from 30 dog breeds were collected from GenBank and used to investigate genetic structure of population. In the analyses of full D-loop sequence variation and the phylogenetic trees constructed by neighbor-joining method, neither haplotypes nor clades specific for any domestic dog breeds were observed. The inter-species sequence variation (4.51%) between domestic dogs and wolves was much higher than the intra-species sequence variation within domestic dogs (1.63%) and wolves (3.64%). The divergence of the dog and wolf occurred approximately 1~2 million years ago based on these values. The taxa of Jeju dog breed in the phylogenetic tree are clustered separately and intermingled with other taxa of breeds, suggesting that active crossbreeding of Jeju dogs with other domestic breeds.

• The Korean Journal of Zoology
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• v.36 no.4
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• pp.545-555
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• 1993

뱀장어속(Anguilla)의 뱀장어(A. ioponica)와 무태장어(A. marmoruta) 및 꾀붕장어(Anago anogo)의 유전적 특징과 종간 유연관계를 밝히기 위하여 isozyme 분석과 mtDNA 분석을 실시하였다. Isozyme 분석결과 20개의 효소 및 비효소단백질에서 총 39개의 유전자를 검출하였고 각종 특유의 genetic marker를 확인하였다. 3종의 평균 유전적 변이는 뱀장어가 HD=0.057. HG=0.065, 무태장어는 HD=0.067 HG=0.053, 체불장어가 HD=0.018. HG=0.020으로 각각 나타났다. 뱀장어와 무태장어의 평균 종간유전적 근인관계는 S=0.420 (D=0.869)로 멀게 나타났다. 6 base를 인지하는 10종류의 제한효소를 처리한 결과 mtDNA 절편양상은 각 종내에서 동일하게 나타났으나 각종간에 차이를 보였고, 종간 평균염기 치 환율은 뱀장어와 무태장어가 p=3.4%, 뱀장어속 2종과 꾀붕장어는 p=9.6%로 나타났다.

• Journal of KIISE:Databases
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• v.30 no.6
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• pp.641-650
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• 2003

The eXtensible Markup Language(XML) is emerging as a standard format of data representation and exchange on the Internet. There have been researches about storing and retrieving XML documents using the relational database which has techniques in full growth about large data processing, recovery, concurrency control and so on. Since in previous systems same structure information and fundamental operation are used for processing of various kinds of XML queries, only some specific query can be efficiently processed not all types of query. In this paper, we propose a flexible query processing system. To process query efficiently, the proposed system analyzes regular path expression queries, and uses $\theta$-join operation using region numbering values to check ancestor-descendent relationship and equi-join operation using parent's region start value to check parent-child relationship. Thus, the proposed system processes efficiently XML regular path expressions. From the experimental results, we show that proposed XML query processing system is more efficient than previous systems.

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.121-126
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• 2012

To determine whether FT-IR spectroscopy combined with multivariate analysis for whole cell extracts can be used to discriminate major leguminous plant at metabolic level, seed extracts of six leguminous plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from seed extracts were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). The PCA could not fully discriminate six leguminous plants, however PLS-DA could successfully discriminate six leguminous plants. The hierarchical dendrogram based on PLS-DA separated the six leguminous plants into four branches. The first branch was consisted of all three Vigna species including Vigna radiata var. radiate, Vigna angularis var. angularis and Vigna unguiculata subsp. Unguiculata. Whereas Pisum sativum var. sativum, Glycine max L and Phaseolus vulgaris var. vulgaris were clustered into a separate branch respectively. The overall results showed that metabolic discrimination system were in accordance with known phylogenic taxonomy. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from seed extracts represented the most probable chemotaxonomical relationship between six leguminous plants.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.384-391
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• 2002

To analyze the genetic relationships among 23 accessions of Asparagus cochinensis $L_{OUR}$, random amplified polymorphic DNA(RAPD) analysis was performed using artificially synthesized 10 primers. The range of polymorphism was $42.9{\sim}91.7%$ with an average of 72.9% in 85 randomly and specifically amplified DNA fragments. On the. basis of similarity coefficient analysis by unweighed pairgroup method, arithmetic average method(UPGMA), 23 accessions of Asparagus cochinensis $L_{OUR}$ could be classified into 6 groups at the similarity coefficient value of 0.82. Group I contained 5 accessions, Group II contained 6 accessions, Group III contained 6 accessions, GroupIV contained 2 accessions, Group V contained 2 accessions and Group VI contained 2 accessions. The range of total genetic similarity coefficient value of 23 accessions of Asparagus cochinchinensis $L_{OUR}$ was $0.47{\sim}0.92$ and average value was 0.76. To obtain more exact data from PCR, we also tried to develope enhanced RAPD techniques using Bovine Lacto Transfer Technique Optimizer(BLOTTO). In RAPD analysis of Asparagus cochinensis $L_{OUR}$, we could obtain better RAPD results by adding BLOTTO at a final concentration of 1%.

• Journal of Digital Convergence
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• v.17 no.11
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• pp.547-556
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• 2019

The purpose of this study was to investigate the relationship between post-traumatic stress and depression in veterans and to examine mediating effects of ego-resilience in relation. Data were collected from 383 subjects of National Merit of the Korean War or Vietnam War with the cooperation of the Daegu Veterinary Association from March to June 2018, and the collected data were analyzed using the SPSS 24.0 Statistics Program to analyze the mediating effects of ego-resilience in post-traumatic stress and depression with hierarchical multiple regression. Post-traumatic stress was found to be high in the case of long absence(F=7.97, p<.001), unemployment(t=2.09, p=.040), poor economic condition(F=64.49, p<.001), and ego-resilience as a parameter in the relationship between post-traumatic stress and depression. Based on the results of this study, it is required to develop various programs focusing on improving ego-resilience and systematic management at the national level in order to intervene in depression of veteran.

• 한국약용작물학회:학술대회논문집
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• 2008.05a
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• pp.302-303
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• 2008

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.336-339
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• 2006

This study was conducted to provide the genetic diversity on Safflower collections and to identify the variations which could be utilized in Safflower breeding. The RAPDs analysis was used to clarify the genetic relationships among 32 Safflower collections. Among 37 primers applied in RAPD analysis, 25 primers that generated appropriate PCR products for identification of the genetic characters in safflower collections were used. Amplified PCR showed the highly reproducible bands at $0.1{\sim}4.0kb$. The number of bands amplified in each primer showed the variations ranging from 1 to 9, with the average of 5.6. A total of 25 bands were identified among twenty-five selected primers and 23 bands (19.2%) showed polymorphism. Based on the similarity value of 0.042 in dendrogram derived from the cluster analysis, the 32 Safflower collections were classified into 6 groups. The two main groups, II and III included 12 collections (38%) and 12 collections (38%), respectively.

• Korean Journal of Plant Taxonomy
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• v.44 no.1
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• pp.51-58
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• 2014

This study aims to define the phylogenetic relationship within Korean section sabina and find molecular markers which resolve the phylogenetic relationship in genus Juniperus and section sabina. cpDNA matK and psbA-trnH were used as molecular markers. The combined analyses of two genes suggested that section sabina was a clade supported by 100% BP. The relationships of [J. chinensis var. sargentii+J. davurica] clade and [J. chinensis var. chinensis+J. chinensis var. procumbens+J. chinensis var. horizontalis] clade were supported by 91% BP and 100% BP, respectively. Thus, the classification of Korean section sabina would be appropriate at follows, (1) J. chinensis var. sargentii+J. davurica, and (2) J. chinensis var. chinensis+J. chinensis var. procumbens According to the results of separate analyses, matK seems to work better resolving power to clarify the phylogenetic ambiguity in Juniperus and section sabina than psbA-trnH.