• Journal of Soil and Groundwater Environment
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• v.14 no.5
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• pp.5-9
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• 2009

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.2212-2216
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• 2008

본 연구는 하도특성의 불규칙으로 인해 수위와 유량이 단일 관계가 형성되지 않은 경우와 유수의 흐름이 지속되어 GZF 측정이 어려운 경우에 구간분리와 GZF를 결정하는 곡선식 개발 방법론이라 할 수 있다. 첫번째 연구과제는 저수위 구간 수위-유량관계곡선식의 GZF 추정방법의 개선에 관한 연구이다. 다음과 같은 연구를 수행하기 위해서 GZF의 변화에 따라 곡선식의 신뢰도를 분석할 수 있는 프로그램을 개발하였다. 본 연구에서 개발한 프로그램은 사용자들이 쉽게 이용할 수 있는 엑셀 VBA(Visual Basic for Applications)로 작성되었으며, 입력자료 구축 모듈, 하도단면 입력 모듈, GZF 설정 모듈, GZF 평가 등의 4개 모듈로 구성되어 있다. 두 번째 연구과제는 구간분리 유무의 기준에 관한 연구로서 수위-유량관계곡선의 신뢰도에 직접적인 영향을 미친다. 본 연구에서는 일차적으로 단면의 특성이 상이한 4개의 수위관측소를 선정하여 수위-면적 곡선과 수위-면적변화량곡선을 생성하였으며 이로부터 단면변화와 구간분리의 특성을 분석하였다. 구간분리의 기준에 영향을 미치는 변수로는 단면특성인자, 유속, 하상경사, 수면경사, 단면통제, 하도통제 등을 들 수 있으며, 또한 다음과 같은 주요변수들이 서로 복합적으로 작용되기 때문에 일정한 기준을 제시하기란 어려운 부분이라 할 수 있다. 따라서 본 연구에서는 구간분리에 영향을 미치는 주요 변수 중에서도 가장 크게 영향을 주는 변수인 하도 단면의 특성 등을 중심으로 연구를 진행하였다. 먼저 단면의 특성이 서로 상이한 수위 관측소 단면을 선정하여 수위관측소별로 저수부에서 고수위 구간까지 10cm의 등간격으로 수위별 면적을 산정하여 구간분리의 가능성을 판단하였다. 구간분리의 유무에 관한 연구는 현재 진행 중에 있으며, 향후에는 1단면, 2단면, 3단면까지 파악하여 단면 특성이 구간분리에 미치는 영향 등을 파악할 계획에 있다. 또한 하도 단면의 다양성을 고려하여 단면형상이 상이한 여러 수위관측소 지점에서 구간분리의 기준을 연구할 계획이며, 단면의 특성을 파악한 후에는 유량, 유속, 하상경사, 하도통제 등을 고려할 계획이다.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.16 no.4
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• pp.371-383
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• 1994

질환성과 관련된 세균의 분포 및 유전자형을 탐색하고자 구강농양 및 골수염의 급성감염 혼자와 진료실 및 실험실의 정상인을 대상으로 시료를 채취하여 포도상구균을 분리 및 동정을 시행하고, 특성을 규명하였으며, plasmid 및 염색유전자를 분리하여 제한효소를 처리후 전기영동을 실시하고 분리된 plasmid로 탐색자를 제작하여 dot blot을 시행하였다. 대부분의 급성환자에서 분리된 포도상구균을 S. lugdunensis와 S. aureus이었으나, 진료실 및 실험실에서는 coagulase 음성 staphylococci가 분리되었다. 급성환자에서 분리된 포도상구균은 ampicillin과 penicillin에 내성을 보였다. 분리된 S. lugdunensis균주중 네 균주는 ${\delta}$형의 용혈소를 생산하였다. Plasmid를 분리한 결과 S. lugdunensis균주중 세 균주는 약 6.5 kilobases이었으나 S. aureus는 약 4.3 kilobases 정도 크기의 band를 보였다. S. lugdunensis에서 분리된 plasmid로 제작한 탐색자로 dot blot를 시행한 결과 치과 영역에서 분리한 plasmid를 갖는 균주는 양성반응을 보였다. 염색체유전자의 유전자형을 분석한 결과 ${\delta}$형의 용혈소를 생산한 네 균주의 S. lugdunensis는 유사한 유전자형을 보였다. 이러한 연구결과 질환의 진행에 S. lugdunensis가 중요한 역할을 하는 것으로 생각되고, 치과영역에 존재하는 plasmid는 공통적인 유전자 서열을 갖는 것으로 추정된다.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 2000.05a
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• pp.153-158
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• 2000

Flow visualization and PIV measurements were conducted to investigate the flow characteristics of multi-channel with inclination angle. The water flow seeded with tiny vegetable powder as tracers revealed details of flow field. The PIV measurement to acquire multi-point velocity data simulatneously was carried out at three space of plates for 5, 10, and 15mm with variation of inlet flow rates of $0.25m^3/h$ and <$0.5m^3/h.$ Experiment results show that space of plates acts a significant role in separating process.

• Clean Technology
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• v.9 no.1
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• pp.9-21
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• 2003

CFC-113 and 1,1,1-TCE which are ozone destruction substances are not used any more in the advanced countries because of Montreal protocol. MC and TCE are now used restrictively at small part of industrial fields in most of countries since they are known to be hazardous or carcinogenic materials. Thus, it is indispensible that the alternative cleaning agents which are environmental-friendly and safe, and show good cleaning ability should be developed or utilized for replacement of the halogenated cleaning agents. Aqueous cleaning agents are evaluated to be promising alternative ones among various alternatives in environmental and economical view point. This study has been carried out as a part of development program of aqueous cleaning agent. First of all, several types of surfactants which are the most important component in aqueous cleaning agents were chosen, and the physical properties, foaming ability, cleaning ability and oil-water separation efficiency of their aqueous solutions were measured and compared for selection of proper type of surfactant in aqueous cleaning agents.

• Korean Chemical Engineering Research
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• v.49 no.6
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• pp.823-828
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• 2011

Characteristics of water separation in a FWKO(Free Water Knok Out) vessel was investigated to remove water from oil-water mixture. Decane, toluene, and asphalt were used as model oils. Preliminary experiments were carried out for decane in a prototype FWKO vessel. Based on the results of preliminary experiments, the prototype vessel was modified and its performance was evaluated by using toluene. The effects of experimental variables on the separation of oil-water mixture were evaluated in terms of separation efficiency. The experimental variables include water cut(water ratio), number of baffles, residence times, and operation temperatures. The optimum conditions of water separation were found to be 0.8 water cut, 3 baffles, 1,200 sec, and $45^{\circ}C$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.73-79
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• 1968

The resolving capacities of xanthophyll pigments on thin-layers of Silica Gel, Hyflo super-Cel, and Micro-Cel C with varying concentrations of acetone in petroleum ether as the developing solvent were compared. The results showed that the resolving capacity of Micro-Cel C thin-layer was superior to others and satisfactory for the separation of leaf carotenoids in clearly separated six bands; carotenes, lutein-zeaxanthin, antheraxanthin, violaxanthin, an unidentified band, and neoxanthin, when it was developed with $13\%$ acetone-petroleum ether solution for 15 to 20minutes in an unsaturated chamber. Adhension of Micro-Cel C to glass was adequate without binder. Calcium sulfate used as a binder appeared to inactivate the resolving capacity of Micro-Cel C. Removing an about 0.2cm-wide layer on bo side of thin-layer slide helped to prevent 'edge effect' which gave tailing and faster solvent ascending along the side than the center. An adequate thickness of thin-layer was obtained when a 3 ml aliquot of the suspension in which l0g powdered Micro-Cel C was suspended in 75 ml distilled water was coated on a $2\times20cm$ glass slide.

• Journal of the Korean Society of Industry Convergence
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• v.24 no.3
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• pp.253-260
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• 2021

The implementation of subsea separation and liquid boosting is becoming a common development scheme for the exploration of deep water fields. Subsea separation is an attractive and economic solution to develop deep offshore fields producing fluid without hydrate or wax. A subsea separator can avoid or simplifying costly surface platforms of floating vessels, as well as being an efficient tool to enhance hydrocarbon production. Subsea separation system should be reliable to ensure successful operation in a wide range of 3-phase flow regime. In this study, multiphase flow characteristics inside in-line type subsea separation system are investigated for the design of subsea separation system.

• Journal of Life Science
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• v.19 no.6
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• pp.728-734
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• 2009

In this work, a simple, inexpensive and reproducible technique of flotation density gradient centrifugation was developed to isolate monocytes with high purity and yield from peripheral blood mononuclear cells (PBMC) using Histopaque solution, density and osmolarity of which were modified to 1.072 g/ml and 335 mOsm with phosphate-buffered saline (PBS) and sodium chloride (NaCl) solution, respectively. The average purity of monocytes was 74.75${\pm}$3.84%, with the individual purity ranging from 71.44% to 82.38%. The average yield of monocytes was 32.62${\pm}$11.16%, with the individual yields ranging from 21.02 to 53.63%. The monocytes isolated by floatation density gradient centrifugation could be successfully cultured into morphologically, phenotypically and functionally dendritic cells in vitro. In conclusion, the entire procedure seemed to be faster and more convenient, simple and cost-effective than other monocyte isolation methods, including plastic adherence and density gradient methods, and has the potential to be developed as a closed system for clinical scale generation of dendritic cells.

• Journal of Advanced Marine Engineering and Technology
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• v.25 no.2
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• pp.338-344
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• 2001

According to the MARPOL 73/78 of Convention, all ships should have oil filtering equipment and 15 ppm bilge which satisfy Requirements of MARPOL 73/78. This study is concerned with designing and manufacturing a prototype Oil content Meter(OCM) used in machinery area of ship. The prototype OCM is composed of two parts which are oil content sensing module and data processing unit. The oil content sensing module consists of infra-red light source, photo-diode light receivers, and a glass tube for bilge water sample. The data processing unit has a micro-processor as hard core and peripheral devices. The experimental results of prototype OCM and analysis of collected data reveal linear property between transmitted light and scattered light as long as the bilge water has low level content of oil. And this linear property leads to a oil content detecting method which is programmed and loaded into the data processing unit. The performance of the prototype OCM is compared with that of the commercial OCM in the market.