• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.7
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• pp.95-104
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• 2018

The structures and energies of the anhydrate and hydrate (hydrate rate: h of 1) states of L-alanine (LA) and glycine (G) were calculated by quantum chemical calculations (QCCs) using B3LYP/6-31G(d,p) for four types of conformers (${\beta}$-extended: ${\Phi}/{\Psi}=t-/t+$, $PP_{II}$: g-/t+, $PP_{II}$-like: g-/g+, and ${\alpha}$-helix: g-/g-). In LA and G, which have an imino proton (NH), three conformation types of ${\beta}$-extended, $PP_{II}$-like, and ${\alpha}$-helix were obtained, and water molecules were inserted mainly between the intra-molecular hydrogen bond of $CO{\cdots}HN$ in $PP_{II}$-like and ${\alpha}$-helix, and attached to the CO group in ${\beta}$-extended. In LA and G, $PP_{II}$-like conformers were most stable in the anhydrate and hydrate states, and the result for LA was different from some experimental and theoretical results from other studies reporting that the main stable conformation of alanine oligopeptide was $PP_{II}$. The formation pattern and stability of the conformation of the oligopeptide was strongly dominated by the presence/absence of intra-molecular hydrogen bonding of $CO{\cdots}HN$, or the presence/absence of an $NH_2$ group in the starting amino acid.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.1
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• pp.14-19
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• 2007

This study was conducted to analyze the antioxidant activity and xanthine oxidase inhibitory effect of hot-water extracts from Elaeagnus multiflora Thunb. to provide fundamental data for the development of functional materials. The antioxidative activities of hot-water extracts from E. multiflora Thunb. were analyzed by electron donating ability (EDA) using 1,1-diphenyl-2-picryl hydrazyl (DPPH), superoxide dismutase (SOD) -like activity by pyrogallol and nitrite scavenging ability. EDA of extracts from unripe, ripe, and overripe fruits were 77.7%, 36.0%, and 23.7% at 100 ${\mu}g/mL$, respectively, and EDA of extract in unripe fruits was higher than those of others. At 1,000 $\mu$g/mL, the SOD-like activities were 32.8%, 11.2% and 5.0% for unripe, ripe, and overripe fruits, respectively. The SOD-like activity was increased along with the increase of unripe and ripe extract concentrations. The nitrite scavenging ability of unripe fruit extracts in 100, 300, 500 ${\mu}g/mL$ of extraction solution in pH 1.2 and 3.0 was higher than those of others. The nitrate scavenging ability of all extracts was decreased according to the increase of pH. Xanthine oxidase inhibitory activities of unripe, ripe and overripe fruits were 30.0%, 28.2%, and 18.2% at 1,000 ${\mu}g/mL$, respectively.

• Korean journal of food and cookery science
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• v.29 no.4
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• pp.407-416
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• 2013

This study was investigated physiological activities of enzyme hydrolysates in 80% ethanol extracts from sesame, black sesame and perilla cake. Total phenol contents in 80% ethanol extracts of sesame, black sesame and perilla cake were 116.90, 102.20 and 141.90 mg/g, respectively. Whereas total flavonoid contents were 64.10, 32.00 and 131.90 mg/g, respectively. Total phenol contents of enzyme hydrolysates in 80% ethanol extracts from sesame, black sesame and perilla cake were 413.30, 221.20 and 409.10 mg/g, respectively. Whereas total flavonoid contents were 361.80, 103.30 and 345.80 mg/g, respectively. Electron donating effects, nitrite-scavenging abilities, ferrous ion chelating effect and SOD-like activities increased by emzymatic hydrolysis. The order of Electron donating effects, nitrite-scavenging abilities and SOD-like activities by emzymatic hydrolysis was perilla > sesame > black sesame cake. And the order of nitrite-scavenging abilities(pH 1.2) was sesame > perilla > black sesame cake.

• Microbiology and Biotechnology Letters
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• v.37 no.3
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• pp.266-272
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• 2009

The annual productions of yam and its aerial bulbils are estimated to 5,000 and 2,500 ton, respectively. But the majority of bulbils had been discarded without specific use. In this study, methanol extract and its organic solvent fractions were prepared from bulbils of Dioscorea batatas Decne, and their antimicrobial, antithrombin, and antioxidant activities were evaluated, respectively. The methanol extract contained 58.98% of water-soluble materials as like yam's extract. But the bulbils's extract contained 12-folds of total polyphenol and 3.4-folds of total flavonoids compared than yam's extract, respectively. For antimicrobial activity the hexane and ethyl acetate fraction showed strong antibacterial activity at $500{\mu}g/disc$ concentration against gram-negative and gram-positive bacteria. Antifungal activity was not observed in any fractions. Strong antithrombin activity was found in the hexane fractions. At 4.8 mg/mL concentration thrombin time (TT) was over 300 sec, which is 4-folds extended than the TT of yam. In a while, the ethyl acetate fraction showed strong DPPH scavenging activity ($IC_{50}$ of $38.1{\mu}g/mL$), SOD-like activity and reducing power, which are comparable to vitamin C or BHT. Our results suggest that the bulbils of yam as yam tuber have useful bio-activities, such as antibacterial, antithrombosis, and antioxidant activity.

• Proceedings of the KAIS Fall Conference
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• 2003.06a
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• pp.320-321
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• 2003

최근 천연물을 중심으로 한 학문이 발전하면서 천연물이 가지는 생리활성 물질에 대한 관심이 중대되고 있다. 또한 인공합성품의 일부가 안정성의 문제가 제기되면서 천연물의 이용분야는 더욱 확대되고 있다. 급속한 경제발전과 생활수준의 향상으로 식생활을 포함한 생활방식의 다양화로 인하여 과거 감염 위주의 질병이 감소하고 선진국형의 만성퇴행성 질환이 증가하는 추세이다. 혈소판은 혈전증(thrombosis)과 지혈증(haemstasis)에서 중요한 역할을 담당하고 있는 인자로서 현관 내 병적 이상으로 인한 과도한 혈전의 생성은 뇌 -심혈관계 질환의 중요한 유발인자로 작용하므로 뇌ㆍ심혈관계 질환이나 항고지혈증을 연구하는데 유용하게 이용되고 있다. 본 연구에서는 토끼 혈액에서 분리한 세정 혈소판 부유액을 이용하여 작약 MeOH 추출물에서 분리한 천연생리활성물질들을 대상으로 혈소판 응집억제활성에 대하여 연구하였다. 작약 MeOH 추출물의 혈소판 응집억제활성 측정에서 강한 혈소판 응집억제활성 작용을 보였다. 따라서 작약 MeOH 추출물을 크로마토그라피법을 이용하여 분리하였고, NMR을 이용한 분광학적 방법으로 지금까지의 분리한 자료와 비교ㆍ분석하였다. Monoterpene glycoside 계열의 성분들인 15개의 compound와 다수의 fraction들을 HPLC를 이용하여 분리하였으며, collagen으로 유도된 혈소판 응집억제활성측정 방법에서 뛰어난 응집억제활성을 보였다. 표준물질을 이용한 HPLC 분석과 ¹H-NMR 관련 자료의 검색을 통하여 최종적으로 compound 1b가 benzoyloxy-paeoniflorin(2.6％). compound 1d가 paeond(1.3％), compound 2c가 albiflorin(3.2％), compound 2e가 paeoniflorin(33.6％)임을 확인할 수 있었다. Compound 3a의 분석결과 benzoyloxypaeoniflorin과 구조적 유사성은 있으나 동일한 구조식으로 확인할 수 없었다. 그러나 collagen에서 응집억제활성이 90％ 이상으로 뛰어난 활성을 나타내므로 benzoyloxypaeoniflorin과 유사한 구조에서 benzoyl group이 다른 작용기로 치환되었거나 R₁ group이 다른 작용기로 치환된 형태로 추측하였다. Benzoyloxypaeoniflorin은 collagen＞thrombin＞U46616＞A.A(arachidonic acid)＞PAF의 순으로 활성을 보였다. 이는 paeoniflorin의 glycoside 5-carbon위치에 위치한 OH기 대신에 benzoyl기로 치환된 benzoyl 기가 혈소판 억제 산물로 작용한 것으로 추측했다. Paeoniflorin.은 U46619＞thrombin＞collagen＞A.A＞PAF순으로 억제를 보였다. Paeoniflorin이 collagen보다 thrombin에서 강한 억제를 보이는 것으로 Ca/sup 2＋/ chelate를 형성함으로 인해 calciu 대사를 저해하는 것으로 추축했다. Compound 3a는 U46619＞collagen＞A.A＞thrombin＞PAF순으로 억제율을 보이므로 이 화합물은 paeoniflorin의 benzoyl기에 OH기가 다른 치환기로 바뀌거나 paeoniflorin의 glycoside 5-carbon 위치의 OH기 대신에 다른 작용기로 치환된 것으로 추정하였다. 이러한 결과로 작약의 주성분인 paeoniflorin과 유사한 구조를 가진 다른 monoterpene glycoside 계열의 화합물들과 비교 분석하고 구조를 화인하고 이들 성분이 어떻게 혈소판 응집억제활성에 작용하는지를 연구하였다.

• Journal of Korea Water Resources Association
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• v.57 no.3
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• pp.165-179
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• 2024

It is crucial to have a comprehensive understanding of inundation and water cycle in urban areas for mitigating flood risks and sustainable water resources management. In this study, we developed a Cellular Automata-based integrated Water cycle model (CAW). A comparative analysis with physics-based and conventional cellular automata-based models was performed in an urban watershed in Portland, USA, to evaluate the adequacy of spatiotemporal inundation simulation in the context of a high-resolution setup. A high similarity was found in the maximum inundation maps by CAW and Weighted Cellular Automata 2 Dimension (WCA2D) model presumably due to the same diffuse wave assumption, showing an average Root-Mean-Square-Error (RMSE) value of 1.3 cm and high scores of binary pattern indices (HR 0.91, FAR 0.02, CSI 0.90). Furthermore, through multiple simulation experiments estimating the effects of land cover and soil conditions on inundation and infiltration, as the impermeability rate increased by 41%, the infiltration decreased by 54% (4.16 mm/m²) while the maximum inundation depth increased by 10% (2.19 mm/m²). It was expected that high-resolution integrated inundation and water cycle analysis considering various land cover and soil conditions in urban areas would be feasible using CAW.

• Korean Chemical Engineering Research
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• v.52 no.3
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• pp.360-365
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• 2014

Vapor-phase hydrogen peroxide(VPHP) has been used as a sterilant in the field of medical and pharmaceutical application due to low corrosive than chlorine contained sterilant. In addition, it is well known that VPHP is effective for decontamination of chemical warfare agents by adding ammonia gas. In this study, the decontamination efficiency was confirmed about CEPS, DFP and dimethoate as simulants of HD, GD and VX using VPHP respectively. For this purpose, VPHP generated from self configured device was injected into decontamination chamber and maintained for reaction time. After the decontamination, the residues are analyzed by GC/MS and decontamination efficiency was calculated. Through by-product for each simulants, the similarities in reaction mechanism of chemical warfare agents were confirmed. CEPS was completely decontaminated at 30% relative humidity within 60 min. By adding ammonia gas, DFP and dimethoate were completely decontaminated within 30 and 150 min respectively.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.22-22
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• 2016

우리나라 하천에서 식생의 변화가 매우 빠르게 진행되고 있다. 최근들어 하천내에서 식생이 차지하는 면적이 증가하는 경향이 뚜렷하게 확인되고 있기 때문이다. 댐 등 하천구조물로 인한 유황변화에 의해 봄철 발아기 유량 및 수위 저하가 발생하고 이로 인해 식생의 유입된다는 것은 이미 많은 연구에서 증명되고 논의된 바 있다. 그러나 댐과 같은 구조물이 없는 자연하천이나 소하천의 경우에도 식생 유입이 빠르게 진행되고 있는 것은 전국적인 경향이라고 할 수 있다. 우리나라 뿐만아니라 일본의 경우에도 대부분의 하천에서 식생이 과도하게 유입되는 현상이 발생하고 있다. 식생의 발생, 발달, 번무 등에 대한 원인은 다양하다고 할 수 있다. 산지지역의 산림 변화로 인한 유사유출 저감, 농업으로 인한 영양염류 증대, 하천정비로 인한 고수부지 확대 등이 주요원인으로 추정되고 있지만 아직까지 정확한 식생 발생 메카니즘에 대한 분석은 이루어지지 않은 상황이다. 일본의 경우 식생의 발생과 진행에 대한 모형을 구축하고 여러 하천에 적용하여 검증하는 연구가 다양하게 진행되고 있지만 지역에 따라 다르게 발생하는 식생의 원인을 단정적으로 결론내리지는 못하고 있는 실정이다. 본 연구에서는 하천내 식생 유입 현황을 분석하고 발생 원인에 대해 검토하였다. 먼저 우리나라 하천에서 식생 발생은 전국적인 현상으로 확인되었다. 과거와 달리 하천의 특정지점에서 식생이 발생하는 것이 아니라 하천의 규모나 지역 등에 관계없이 전국적으로 식생이 발생하고 있는 것이 식생면적 분석으로 확인되었다. 또한 식생 면적이 최근 들어 더 증가하고 있음도 확인되었다. 본 연구에서는 강우의 연도별 변화가 식생발생에 미치는 영향을 분석하였다. 봄철(4월, 5월, 6월)의 연도별 강우량 변화가 식생 발생에 미치는 영향을 분석하였는데 이 기간 동안의 강우가 줄어듦에 따라 식생 발생이 더 활발하게 진행될 수 있는 것으로 나타났다. 또한 봄철의 가뭄과 여름철 홍수 미발생이 이어지는 경우 식생이 더 활발하게 발생할 수 있는 것으로 나타났다. 하천 구조물에 의한 하천유황 변화가 식생 발생에 큰 영향을 미치듯이 강우 발생 양상의 변화로 인한 유황 변화가 식생 발생에 영향을 미칠 수 있는 것으로 나타난 것이다. 또한 일본의 경우에도 이와 같은 경향이 유사하게 나타나는 것으로 분석되었다. 일본의 식생조사 결과와 강우 패턴을 비교한 결과 우리나라와 같이 일본에서도 유사한 경향이 나타났다. 본 연구 결과로 추정해 볼 때 강우 패터의 변화가 식생의 유입에 큰 영향을 미치며, 특히 기후변화로 인해 발생하는 봄철 강우의 감소가 주요원인중 하나로 추정될 수 있다고 할 수 있다.

• Journal of the Earthquake Engineering Society of Korea
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• v.8 no.5 s.39
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• pp.35-43
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• 2004

Small-scale models have been frequently used for seismic performance tests because of limited testing facilities and economic reasons. However, there are not enough studies on similitude law for analogizing prototype structures accurately with small-scale models, although conventional similitude law based on geometry is not well consistent in the inelastic seismic behavior. When fabricating prototype and small-scale model of reinforced concrete structures by using the same material, added mass is demanded from a volumetric change and scale factor could be limited due to aggregate size. Therefore, it is desirable that different material is used for small-scale models. Thus, a modified similitude law could be derived depending on geometric scale factor, equivalent modulus ratio and ultimate strain ratio. In this study, compressive strength tests are conducted to analyze the equivalent modulus ratio of micro-concrete to normal-concrete. Then, equivalent modulus ratios are divided into multi-phase damage levels, which are basically dependent on ultimate strain level. Therefore, an algorithm adaptable to the pseudodynamic test, considering equivalent multi-phase similitude law based on seismic damage levels, is developed. Test specimens, consisted of prototype structures and 1/5 scaled models as a reinforced concrete column, were designed and fabricated based on the equivalent modulus ratios already defined. Finally quasistatic and pseudodynamic tests on the specimens are carried out using constant and variable modulus ratios, and correlation between prototype and small-scale model is investigated based on their test results. It is confirmed that the equivalent multi-phase similitude law proposed in this study could be suitable for seismic performance tests on small-scale models.

• Journal of the Earthquake Engineering Society of Korea
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• v.7 no.6
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• pp.49-57
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• 2003

Although there are several experimental techniques to evaluate the seismic behavior and performance of civil structures, small-scale models in mast of physical tests, instead of prototypes or large-scale models, would be used due to a limitation on capacities of testing equipments. However, the inelastic seismic response prediction of small-scale models has some discrepancies inherently because the similitude law is generally derived in the elastic range. Thus, a special attention is required to regard the seismic behavior of small-scale models as one of prototypes. In this paper, differences between prototypes and small-scale models pseudodynamically tested on steel column specimens are investigated and an alternative to minimize them is suggested. In general, small-scale models could have the distorted stiffness induced from some experimental errors on test setup, steel fabrication and so on. Therefore, a modified similitude law considering both a scale factor for length and a stiffness ratio of small-scale model to prototype is proposed. Using the modified similitude law to compensate experimental errors, the pseudodynamic test results from modified small-scale model are much improved as compared with the results of prototype. According to the pseudodynamic test results of small-scale steel models, it can be concluded that the modified similitude law proposed could be effective in simulating the seismic response of prototype structures.