• Korean Journal of Microbiology
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• v.48 no.2
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• pp.87-92
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• 2012

The purpose of this study was to examine the cellular response of Salmonella typhimurium exposed to tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis L.). TPP showed a dose-dependent bactericidal effect on S. typhimurium. Analysis of cell membrane fatty acids of S. typhimurium cultures treated with TPP identified unique changes in saturated and unsaturated fatty acids, while scanning electron microscopic analysis demonstrated the presence of perforations and irregular rod forms with wrinkled surfaces in cells treated with TPP. Two-dimensional polyacrylamide gel electrophoresis of soluble protein fractions from S. typhimurium cultures showed 16 protein spots increased by TPP. These up-regulated proteins including proteins involved in antioxidants and chaperons, transcript and binding proteins, energy and DNA metabolism were identified by peptide mass fingerprinting using MALDI-TOF. These results provide clues for understanding the mechanism of TPP induced stress and cytotoxicity on S. typhimurium.

• Analytical Science and Technology
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• v.24 no.3
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• pp.159-167
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• 2011

In this study, the hydride vapour generator inductively coupled plasma mass spectrometry (HVGICPMS) was applied as the new analytical method to show the high accurate and reproductive data analysing the amounts of selenium and germanium being existed inside a system of plant. In order to decrease the interference effects, such as ion and molecular interference. Mini torch was used into the ICPMS instead of the conventional torch. At conditions of the different kinds and concentrations of acid solution, the different reductive conditions for composing hydride, and the different methods for making ash, the contents of selenium and germanium in lettuce were analysed. The inspection of yields and data comparison from SRM-1574 and -1570a were used for increasing the accuracy of this analysis.

• Korean Journal of Animal Reproduction
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• v.18 no.3
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• pp.191-197
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• 1994

It is generally known that mutations in any of several genes encoding photoreceptor-specific proteins have resulted in retinitis pigmentosa (RP), a disease characterized by losing photoreceptor function with progressive degeneration of photoreceptor cells and eventually leading to blindness. To study the procure and cure of photoreceptor degeneration, we produced transgenic mice. Transgene consisted of a 12.5kb genomic DNA fragment that contains mutated pig rhodopsin gene (Pro-347-Ser) including both the 5'-franking (4.0 kb) and the 3'-franking (2.9 kb) sequences. This gene was used for the production of transgenic mouse. The mutated rhodopsin DNA was microinjected into male pronuclei of fertilized mouse (C57BL /6]) embryos. We detected transgenic animals harboring mutated rhodopsin gene by PCR and Southern blot analysis. These transgenic mice showed stable transmission of microinjected rhodopsin gene into their offspring. Therefore these animals will provide a novel approach to study the mechanism of the photoreceptor degeneration and be provided as a disease model for the treatment of the blind in human.

• Applied Biological Chemistry
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• v.35 no.1
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• pp.14-22
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• 1992

A series of new N-[1-(benzotriazol-1-yl)aryl]arylamine derivatives were synthesized and their antifungal activities $(pI_{50})$ in vitro against Pyricularia oryzae, Fusarium oxysporum f. sp. sesami, Valsa ceratosperma and Botrytis cinerea were dertermined by the agar medium dilution method. From the results of the quantitative structure-activity relationships $(QSAR_S)$ analysis, $hydrophobicity({\pi})$, $electronic({\Sigma\sigma})$ and molar $refractivity({\Sigma}M_R)$ parameter of X & Y-substituents on the phenyl group were also shown to be important factor in determining the variation in the antifungal activity. 4-Bromo group substituents (1d & 2b) were the most effective compounds and the $half-life(T_{1/2})$ on the hydrolysis of X(1) at netural pH was about 1.5 day. Molecular orbital(MO) functions of substrate compound, linear free energy relationships$(LFER_S)$ on the antifungal reactivity arid the results of molecular design were also discussed.

• Journal of the Earthquake Engineering Society of Korea
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• v.2 no.1
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• pp.95-111
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• 1998

In this study, the concepts and procedures of the seismic damage analysis methods are examined for both the element-level and the system-level. The seismic damage analysis at the element-level is performed for several example structures using existing method for structural elements or single-degree-of-freedom (SDOF) systems such as the Park and Ang method. In order to analyze seismic damage at the system-level, two types of procedures are used. In the first type of procedure, the system-level seismic responses can be estimated by using the system representative response method(SRRM), or the equivalent SDOF system response method (ESDOF-SRM). Then, the system-level seismic damage is analyzed from the system-level seismic responses using existing method for structural elements or SDOF systems. IN the second type of procedure, the system-level seismic damages are analyzed using the element damage combination method (EDCM) combing the element-level damage indices determined by existing method. To compare tendency of the seismic damage analysis using each methods, example analysis is accomplished for several cases of different structures and different earthquake excitation.

• The Journal of the Convergence on Culture Technology
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• v.7 no.2
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• pp.389-394
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• 2021

In this work, we study the properties of molecular structure and boundary orbital functions of the DPHMP-AlH and propiophenone and butyrophenone, which are forms of alkoxy-amine-aluminum derivatives. Furthermore, we investigate the effect on the selective reduction of the final products (R), (S)-phenylpropanol and (R), (S)-phenylbutanol by calculating the stereoscopic and thermodynamic parameters of the transition state. Considering the three-dimensional molecular structural stability, the transition status of (S) types DPHMP-AlH and alkylphenone was found to be more stable, resulting in the selective reductions of DPHM-AlH and alkylphenone from this result: (S)-(1)-phenylpropanol and (S)-(1)-phenylbutanol was confirmed that the formation was advantageous.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.4
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• pp.769-777
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• 2015

In this study, the rheological models were introduced and developed to reflect rate dependent tensile behaviour of concrete. In general, mechanical properties(e.g. strength, elasticity, and fracture energy) of concrete are increased under high loading rates. The strength of concrete shows high rate dependency among its mechanical properties, and the tensile strength has higher rate dependency than the compressional strength. To simulate the rate dependency of concrete, original spring set of RBSN(Rigid-Body- Spring-Network) model was adjusted with viscous and friction units(e.g. dashpot and Coulomb friction component). Three types of models( 1) visco-elastic, 2) visco-plastic, and 3) visco-elasto- plastic damage models) are considered, and the constitutive relationships for the models are derived. For validation purpose, direct tensile test were simulated, and characteristics of the three different rheological models were compared with experimental stress-strain responses. Simulation result of the developed visco-elasto-plastic damage(VEPD) model demonstrated well describing and fitting with experimental results.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.136-136
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• 2011

AlSb는 광전자 소자응용에 매우 유용한 재료이며 이를 이용한 반도체소자 설계 및 밴드갭 엔지니어링을 위해서는 화합물 반도체의 전자밴드구조를 포함한 광학적 특성이 반드시 요구된다. 본 연구는 이러한 요구의 해결방안으로서 AlSb 화합물의 유전함수 온도의존성을 0.7~5.0 eV의 에너지 영역에서 타원편광분석법을 이용하여 분석하였다. AlSb는 산소와 급격히 반응하기 때문에, 대기 중에서 물질 고유의 광특성이 유지되기 어려울 뿐만 아니라, 박막 위에 생성되는 산화막 때문에 순수한 AlSb의 유전함수 측정이 불가능하다. 따라서 박막의 산화 효과를 최소화하기 위하여 초고진공 상태의 molecular beam epitaxy 챔버 안에서 800 K의 온도로 성장한 1.5 ${\mu}m$ 두께의 AlSb 박막을 상온 300 K 까지 온도를 단계적으로 변화시켜가며 타원편광분석기를 이용하여 실시간으로 측정하였다. 각 온도에서 측정된 AlSb의 유전함수를 2차 미분하여 전이점(critical point)을 분석한 결과 $E_0$, $E_0+{\Delta}_0$, $E_1$, $E_1+{\Delta}_1$, $E_0'$, $E_0'+{\Delta}_0'$, $E_2$, $E_2+{\Delta}_2$에 해당하는 각 전이점들의 온도 의존성을 확인할 수 있었다. 실험에서 측정된 특정 온도를 포함하여 임의의 온도에서의 AlSb의 유전함수를 유도하기 위하여 변수화모델을 사용하였고 이를 통하여 각 변수들의 온도 의존 궤적을 분석하였다. 2차 미분법을 이용한 전이점들의 온도의존성 분석결과를 기준으로 변수화 모델링을 진행하였으며 그 결과 각 온도에서 실제 유전함수와 근소한 차이를 갖는 AlSb의 유전함수 모델을 만들 수 있었다. 따라서 본 연구결과는 반도체 물성에 대한 학술적 측면뿐 아니라 고온에서의 소자공정 실시간 모니터링 및 반도체 소자 설계 등의 산업적 측면에서 매우 유용하게 사용될 것으로 기대된다.

• Journal of the Korean Society for Marine Environment & Energy
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• v.20 no.2
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• pp.100-106
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• 2017

Artificial upwelling structure has been set up in sea mount. Bottom water can upwelling euphotic zone. Plentiful nutrient included in bottom water could not only enhance primary production but also expect food chain reaction and gathering fish. This study explain flowing features subject to porosity changes according to the material and shape of artificial upwelling structure. As a result, the upward flux is getting decreased while the porosity is increasing. And it figured out when the upward flux was decreased, the downward flux was also decreased. Futhermore, it was confirmed that the best efficiency of upwelling flux was shown up when the porosity was 10% according to the volume of artificial upwelling structure in case of 20% of porosity, it also has a good efficiency in comparison with impermeable artificial upwelling structure. Therefore, to build the artificial upwelling structure, It is encouraged to design it less than 20% of porosity for the best performance.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.1
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• pp.253-259
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• 2015

In this paper, we propose a transformer of alternating voltage source utilizing a semiconductor, operating in bidirectional fashion. Transformer is a device transferring energy by inductive coupling between its winding circuits. Conventional transformer is a device, composed of a primary coil and a secondary coil, transforming an alternating voltage. The system we propose is designed with a single circuit transforming the level of voltage signal in two ways; from the source to the load, and vice versa. For semiconductor switches, the NPN transistor is connected to the alternating voltage source terminal, and emitter terminal is connected to the inductor in the system as an energy storage element. The control signal is applied to the base terminal of the semiconductors. We have shown that the system we propose, by adopting only one circuit, drives an alternating voltage transformer that changes the amplitudes of voltage signal in reciprocal way.