• KSBB Journal
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• v.18 no.6
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• pp.473-478
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• 2003

The purpose of this study is to look for the optimal conditions of methane production. The conditions tested for methane production enhancement were temperature, pH, carbon source, nitrogen source, and inhibitor which can affects methane production. As a result, optimal conditions for methane production were 30$^{\circ}C$, neutral pH, methanol as a carbon source, NH$_4$Cl as a nitrogen source. 2-Bromoethanesulfonic acid was used as an inhibitor which can affects methane production. Existence in broth less than 10mM, inhibited methane production. Organic acid measurements revealed that formic acid exists in broth as majority.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.2
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• pp.128-136
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• 1998

Most of total nitrogen in the surface soil exists in organic forms, of which amino acid-N is the major fraction. By-product fertilizers provide soil with humic substances, and humic acid is an essential component of humus. Amino acids(AAs) are easily converted to inorganic-N forms and thus play an important role in N fertility. This experiment was conducted to investigate the contents and distributions of AAs in humic acids which were extracted from the commercial by-product fertilizers of different composting materials. Total contents of AAs in humic acids ranged from 1.2 to 5.6%, of which neutral AAs were the highest with ranges of 0.8~4.5%. AAs contents in fertilizers composted from the plant residues such as leaf litter, sawdust and bark were in an order of neutral>acidic>basic AAs. In contrast, those from animal wastes, such as poultry and pig manures, were in an order of neutral>basic>acidic AAs. Distributions of total, acidic and neutral AAs were in the respective order of leaf litter>sawdust>pig manure>poultry manure>peat, bark>sawdust>leaf litter>peat and leaf litter>sawdust>bark>peat. Distributions of the basic AAs were in the reversed order of the acidic AAs. In bark fertilizer with increasing compost maturity, contents of the acidic AAs were increased in compensation for the decreases in those of neutral and basic AAs. Results demonstrated that distributions of amino acids in humic acid of by-product fertilizers were different from composting raw materials and degrees of humification.

• Korean Journal of Microbiology
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• v.37 no.4
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• pp.317-324
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• 2001

We have previously developed three stage methane fermentation system capable of digesting food wastes effectively and then releasing high organic wastewater as a final product. In this study, we tried to devise an ecological water treatment system, which can efficiently remove the nitrogen and phosphorus contained in the organic wastewater. The system was made of microbiological filters, algae, and waterfleas. Of two species of alga tested, Selenastrum capricornutum showed higher growth rate and more efficiently removed the nitrogen from the wastewater than by Chlorella sp. In addition, the highest growth rate and the nitrogen removal efficiency could be obtained when high concentrations of $Mg^{2+}\; and\; Ca^{2+}$ were added to the diluted wastewater and the molar ratio of nitrogen to phosphorus was adjusted to 10 : 1. In this study the population relationship between alga and water flea was also examined in a test tube. The initial number of algal cells decreased as the waterflea population increased. However, the number of algal cells gradually increased again when waterflea population decreased partly due to the environmental resistance. From these results, it was believed that the ecological water treatment system could be used for removing the nitrogen and phosphorus from organic wastewater very effectively. Moreover, the waterflea cultured by this system as a final predator could be used as a good foodstuff for fishes.

• The Korean Journal of Mycology
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• v.47 no.1
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• pp.1-12
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• 2019

This study was conducted to investigate the optimum culture conditions, for mass production of Tremella fuciformis in M9 basic medium. The strain KMCC04674, used in this study was identified T. fuciformis by internal transcribed spacer (ITS). To define the optimum conditions for the mass production of T. fuciformis, we investigated the effects of different culture conditions and various nutrient sources on the fungal growth. The optimum initial pH and temperature for the fungal growth were 5.0 and $25^{\circ}C$, respectively. The optimal composition of the growth medium was 4.0% mannitol, 3.0% $NH_4H_2PO_4$, 1.0% malt extract, 1.0% glutamic acid, 5mM $CaCl_2$, and 0.5% glucanic acid.

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.12a
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• pp.301-301
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• 2009

겨울철 농경지 이용율 제고 및 자연경관 조성을 위하여 녹비작물을 재배하는 면적이 지속적으로 증가하고 있는 추세이다. 그 동안 녹비작물에 대한 연구는 주로 파종적기, 작물에 대한 양분공급효과 및 타감 효과 등에 집중되어왔다. 본 연구에서는 토양 중 양분공급 및 미생물활성의 경시적 변화, 잡초억제 및 작물생육에 대한 녹비작물의 투입효과를 알아보기 위하여 수행하였다. 국립농업과학원 구내포장에서 2007년 9월 하순에 헤어리베치(8kg/10a)와 호밀(10kg/10a) 종자를 파종한 후, 이듬해 4월 하순에 수확하여 토양에 환원하였다. 질소공급량은 화학비료(15.2kgN/10a) 대비 헤어리베치(32.2kg/10a, 212%), 호밀(9.2kg/10a, 60%) 및 헤어리베치+호밀(15.5kg/10a, 102%)로 헤어리베치의 질소공급효과가 매우 높았다. 토양 무기태질소 함량은 녹비 처리 후 30일경에 최대에 도달하였으나, 호밀 처리구는 조사기간 동안 큰 변화가 없었다. 토양 유기물 함량의 변화는 시험 전(10DBI)과 비교하여 큰 차이를 보이지 않았으나, 녹비환원 후 10일경에 일시적으로 감소하는 경향을 보였는데 이는 녹비환원 후 토양경운에 의한 영향으로 판단되며, 무기태질소와는 달리 호밀처리구의 유기물함량이 지속적으로 증가하는 경향을 보였다. 토양 탄수화물(수용성 당)은 시험 전에 가장 높았으며, 시간의 경과와 함께 서서히 감소되는 양상을 보였으며 토양 미생물탄소 및 미생물질소의 양은 녹비처리구가 화학비료 처리구에 비해 높았으며 녹비환원 후 50일 까지는 증가하다가 그 이후에 감소하는 경향을 보였는데 이는 토양의 무기태질소 및 탄수화물과 밀접한 관계가 있는 것으로 판단되었다. 녹비처리 후 85일경에 잡초발생량을 조사한 결과, 화학비료 처리구에 비하여 헤어리베치 처리구는 40%, 호밀 처리구는 68%, 헤어리베치+호밀 처리구는 40%의 잡초억제효과가 있었다. 고추과의 수량은 화학비료 처리구(702kg/10a)>헤어리베치(694kg>10a)>헤어리베치+호밀(361kg/10a)>호밀(179kg/10a)의 순으로 나타났다.

• Applied Biological Chemistry
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• v.14 no.1
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• pp.99-102
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• 1971

These experiment were conducted to study the effects of various nitrogen sources and inorganic salts upon the growth of Saccharomyces rouxii T-9, and the results obtained were as follows: 1) The strain showed the most rapid growth in the case of 2.5 to 5.0% addition of peptone as an organic nitrogen source, and 1.0% addition of $(NH_4)_2HPO_4$ as an inorganic nitrogen source respectively to the media containing no NaCl. 2) Its growth was the most rapid in the case of 1.5% addition of yeast extracts as an organic nitrogen sources, and 5% addition of urea as an inorganic nitrogen source respectively to the media containing 20% of NaCl. 3) Its growth was the most rapid in the case of 1% addition of $NaNO_3$ as an inorganic salt to the media containing no NaCl, while to the media containing 20% or 26% of NaCl its growth was more rapid in the case of 5% addition of $NaNO_3$ than 1% addition. 4) Its growth was more rapid in the case of 0.5% addition of $MgSO_4\;and\;CaCl_2$ respectively to the media containing 20% of NaCl than to the media containing no NaCl, and was rapid in the case of 1% addition of $MgCl_2$ to the media containing 26% of NaCl.

• Korean Journal of Organic Agriculture
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• v.22 no.4
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• pp.719-730
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• 2014

This study was conducted to select proper soil amendments in order to reduce the occurrence of potato scab and maintaining soil health by applications of dolomitic lime, sulfur, potassium sulfate, shell meal fertilizer, silicate fertilizer, lime nitrogen and ammonium sulfate fertilizer in different pH levels of volcanic ash soil with continuous cultivation of potato. In potassium sulfate-applied plot with a low soil pH, the incidence rate and disease severity of scab were lowest at 84.4% and 28.4%, respectively. Those were lowest among the treatments. Value of potato scab control was 12.3% and marketable yield of potato was highest at 93.2%. In lime nitrogen-applied plot (60 kg/10a), the incidence rate was low at 38.3%, and control value was 23.8% and marketable yield of potato was high at 66.3%. In relatively higher pH soils, the incidence rate of scab was lowest at 38.3% in the lime nitrogen-applied plot (60 kg/10a). Value of potato scab control was 23.8%, which was four times higher than that in sulfur-applied plot. Marketable yield of potato was highest at 66.3% in the lime nitrogen-applied plot. In the lime nitrogen plot infected with potato scab pathogen such as S. acidiscabies and S. scabiei were remarkably lower than other soil amendments at 2.5, 5, and 10 g/L concentrations of lime nitrogen using Glucose Yeast Malt (GYM) medium. In conclusion, this study suggests that potassium sulfate application in low pH soil (less than pH 5) and lime nitrogen application in relatively higher pH soil (more than pH 6) before potato seeding might be helpful to reduce the occurrence of potato scab.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.04a
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• pp.94-98
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• 2005

강변여과방식의 시험 취수가 진행 중에 있는 범람원지역의 산화-환원 반응을 밝히기 위해 10개의 간이 관측정을 설치하여 수질 모니터링을 수행하였다. 분석 결과 하천수에 인접한 지역에서 강한 환원환경이 생성되는 것을 확인하였으며, 이는 하천수 내 용존 유기 탄소가 인근 범람원 지역에 퇴적 산화되어 나타난 결과였다. 즉 퇴적된 용존 유기 탄소가 감소하면서 용존 산소와 질산성 질소는 감소시키지만, 이러한 환경변화가 수처리 시 문제가 될 수 있는 용존 망간의 농도를 증가시키게 되며, 따라서 향후 이 지역에 대한 추가적인 분석과 함께 집수정으로 통해 유출되는 용존 망간에 대한 대책이 필요한 것으로 조사되었다.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2014.11a
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• pp.152-152
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• 2014

본 연구에서는 핫플레이트를 이용한 간단한 산화아연 박막의 열처리를 통해 역구조 유기 태양전지에서의 광전환 효율증가를 소개한다. 유기태양전지는 환경오염에 따른 신 재생에너지의 필요성이 대두되면서, 중요한 주제가 되고 있다. 본 연구에서는 3차원 물결구조의 산화아연 박막을 핫플레이트를 이용하여, $180^{\circ}C$ 에서 산화아연의 3차원 물결구조의 형성에 성공하였다. 그리고 구조적 측면 뿐만 아니라, 아연 용액에 포함된 질소로 인해 산화아연의 밴드갭이 조절되어 유기태양전지의 효율이 증가했음을 확인 하였다. 또한 본 연구의 공정을 이용하여 PET 유연기판으로의 적용이 가능하다는 것을 확인 하였다.

• Journal of the Korea Organic Resources Recycling Association
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• v.12 no.3
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• pp.119-125
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• 2004

Sugar beet stillages were used as a substrate for the production of single cell protein by a thermotolerant yeast Candida rugosa. 3 Stillage substrates were nutritionally optimized for the better production of yeast biomass and for the reduction of COD. The addition of Phosphorus(P) was required for all stillages, but Nitrogen(N) only when the residual sugar remained. The addition of P increased the biomass production to 23-61%. The addition of N increased the biomass production only a little, but when added together with P increased to 90%. The COD decreased to 26-46% when P was added, but decreased to 85% when P was added together with N.