• Journal of the Korean Chemical Society
• /
• v.22 no.5
• /
• pp.320-325
• /
• 1978

In order to elucidate the mechanism associated with the solvent extraction of uranium(VI) using DEPA and DPPA as extractant the uranium(VI) complexes formed during the solvent extraction were isolated and characterized by means of IR, NMR, chemical analysis and molecular weight determination. It has been found that uranium(VI) replaces the acidic hydrogen ions of the extractants DEPA and DPPA to form chelated polynuclear complexes, the molecular weight of U(VI)-DEPA complex being $2.1{\times}10^4$. The isolated U(VI)-DEPA complex has been found to be the same chemical species as is formed during the solvent extraction process. In case of DEPA the distribution coefficient of uranium is the largest of the pure aqueous uranium solution and is increasing for the acidic solutions in the order of $H_3PO_4.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.9 no.1
• /
• pp.99-108
• /
• 2001

The study was carried out to evaluate characteristics of inorganic phosphate fractions in cultivating soil with paper mill sludge comport(PMSC). The experiment were conducted in cultivating corn by a pot test. The place of the pot test was a plastic house. The pot size was 1/5000a and PMSC was added at rates of 0, 30, and 100 Mg/ha. The pot experiment was divided to two treatments, one in which corn was cultivated and the other which was not. The items of measurement was pH and inorganic phosphate fractions which were determined for the pot tests for 80 days. The results can be concluded as follows, pH increased depending on the higher rates of PMSC application to the soil during the Pot tests, because calcium content was high and contents of nutrient were high. When the application rates of PMSC were increased, Saloid-P and Fe-P decreased, while Al-P and Ca-P in soil increased during the pot tests. According to the increase of PMSC application rates, the growth and the yield of com significantly increased Such results seem to be associated with the higher nutrient level from PMSC application. Although uptake of phosphorus in corn decreased in the corn with increased application of the PMSC, phosphate deficiency didn't appear in the pot test.

• Korean Journal of Microbiology
• /
• v.42 no.3
• /
• pp.223-229
• /
• 2006

To develop multifunctional microbial inoculant, an insluble phosphate-solubilizing bacterium with antifungal activity was isolated from plant rhizospheric soil. On the basis of its morphological, cultural and physiological characteristics and Biolog analysis, this bacterium was identified as Pseudomonas fluorescens RAF15. P. fluorescens RAF15 showed antifungal activities against phytopathogenic fungi Botrytis cinerea and Rhizoctonia solani. The optimal medium composition and cultural conditions for the solubilization of insoluble phosphate by P. fluorescens RAF15 were 1.5% of glucose, 0.005% of urea, 0.3% $MgCl_2{\cdot}6H_2\;0.01%\;of\;MgSO_4{\cdot}7H_2O\;0.01%,\;of\;CaCl_2{\cdot}2H_2O$, and 0.05% of NaCl along with initial pH 7.0 at $30^{\circ}C$. The soluble phosphate production under optimum condition was 863 mg/L after 5 days of cultivation. The solubilization of insoluble phosphates was associated with a drop in the pH of the culture medium. P. fluorescens RAF15 showed resistance against different environmental stresses like $10-35^{\circ}C$ temperature, 1-4% salt concentration and pH 2-11 range. The strain produced soluble phosphate to the culture broth with the concentrations of 971-1121 mg/L against $CaHPO_4$, 791-908 mg/L against $Ca_3(PO_4){_2}$, and 844 mg/L against hydroxyapatite, respectively. However, the strain produced soluble phosphate to the culture broth with the concentrations of 15 mg/L against $FePO_4$, and 5 mg/L against $AlPO_4$, respectively.

• Korean Journal of Organic Agriculture
• /
• v.5 no.2
• /
• pp.71-84
• /
• 1997

유기농산물은 질 좋은 농산물이며 그 생산농법이 환경친화적인 것으로 이해하고 있어 참여농가수도 증가일로에 있고 소비자들에 의한 유기농산물 구입 역시 점차 증가 추세에 있으나, 한국유기농업은 국제적 유기농업에 다르게 잘못 정의된 채 확산되고 있어, 그 핵심기술의 환경영향평가의 과학화에 대한 시대적 요청과 유기농업 기본규약 제정 필요성이 학계 일부에서 제기 되고 있는 등 한국유기농업은 최근 발전을 위한 진통을 겪고 있다. 이에 본 논문은 한국유기농업이 태동된 배경과 70년대 말로부터 80년대 초에 걸쳐 태생된 한국유기농업이 국민적 관심을 얻게된 배경과 정농회, 한국유기농업협회, 한국자연농업협회, 한 살림 등의 유기농업단체와 한국유기농업학회, 환경보전형농업생산소비단체협의회, 흙살림연구소 등의 발전과정 및 활동을 소개하고 있다. 또한 서울에서 개최된 IFOAM Asia대회, 한국자연농업협회의“자연농업연구소”, 한국유기농업협회의“건강과 자연농업”등에 관해서 뿐만 아니라 우리 정부의 21세기를 향한 농림환경정책중 유기농업부문에 대한 각종 장기지원책, 즉 중소농대책의 일환의 유기-자연농업 지원책, 유기농산물에 대한 품질인증제 및 팔당상수원보호구역내 유기농업지원 등이 소개되고 있다. 한편 한국유기농업기술의 핵심내용이었던 유기질비료 과다시용 문제가“유기농산물 채소의 고 질산염 함량”으로 촉발된 식품안정성 논쟁 후에 유기질비료의 추천시용량이 다다익선적 개념에서 80t/㏊으로 낮아졌다가 다시 50t/㏊으로 낮아졌으며 1996년 6월부터는 20t/㏊로 크게 낮추어 졌음을 보고하고 있다. 또한 일부 선도적 유기독농가의 경험적 기술이 토대가 되어 성립된 현 유기농업기술은 과학적 기술검증을 거쳐야 함과 퇴비사용 만으로 실시하는 현 유기농업 기술에 의해 야기되는 근권내 질산 미치 인산염 집적을 회피하기 위해서는 윤작, 녹비작물 및 두과작물의 재배로 토양비옥도 향상을 통한 유기농업 기술 개발이 필요하며 IFOAM 기본규약과 FAO/WHO Codex의 핵심 내용을 수용하는 한국유기농업 기본규약 제정이 제정이 절실히 요구됨을 강조하고 있다. 마지막으로 한국유기농업의 핵심기술에 대한 환경영향평가와 기본규약제정 필요성을 역설하고 한국유기농업의 과학화와 질 좋은 유기농산물 생산을 위해 노력하고 있는 소비자 문제를 연구하는 시민의 모임 등 몇몇 단체들의 최근 활동이 언급되고 유기농업에 대한 농과대학내 연구와 강의 등이 소개되었다.

• Korean Journal of Soil Science and Fertilizer
• /
• v.42 no.1
• /
• pp.29-36
• /
• 2009

Fourteen bacterial strains were isolated from crop rhizosphere and identified as phosphate solubilizing bacteria (PSB) by 16S rRNA analysis. Only 3 strains exhibited a strong ability to solubilize insoluble phosphate in agar medium containing a hydroxyapatite. The rates of P solubilization by isolates were ranged from 200 and $2300\;mg\;L^{-1}$, which are inversely correlated with pH in culture medium. Furthermore, HPLC analyses reveal the production of organic acid from the culture filtrates of PSB. Among these, strain Acinetobacter sp. released only gluconic acid, Pseudomonas orientalis produced gluconic acid which was subsequently converted into 2-ketogluconic acid, and Enterobacter asburiae released acetic acid and succinic acid. On the other hand, P. orientalis and E. asburiae released $372\;mg\;L^{-1}$ and $191\;mg\;L^{-1}$ of IAA into broth culture, respectively, while Acinetobacter sp. did not produce IAA. Furthermore, in vivo study showed that plant growth promoting effect by bacteria generally seemed to be increased IAA production and phosphate solubilization.

• Korean Journal of Organic Agriculture
• /
• v.16 no.1
• /
• pp.115-125
• /
• 2008

This study was conducted to determine adequate forage crop choice and optimal level of livestock manure, when different types and levels of the livestock manure were applied in corns or sorghum$\times$sorghum hybrids for the production of organic roughages by utilizing livestock manure. For the corn, yields of annual dry matter (DM) and total digestible nutrients (TDN) were highest in N+P+K-applied treatments, showing 17.3 and 11.7 ton/ha, respectively. Treatments applied 100% composted cattle manure (8.9 and 6.1 ton/ha) and 100% cattle slurry (9.4 and 7.5 ton/ ha) in contrast with chemical fertilizer-N had higher yields of DM and TDN than no fertilizer (4.8 and 2.7 ton/ha) and P+K-applied treatments (8.8 and 6.0 ton/ha). Particularly, treatments applied 150% composted cattle manure and 150% cattle slurry were markedly higher, which represented 11.4 and 7.6 ton/ha and 10.3 and 7.3 ton/ha, respectively. Crude protein (CP) contents for corns applied livestock manure ranged from 5.6 to 6.6%, which were significantly (p<0.05) higher than those of no fertilizer (3.9%) and P+K-applied treatments (5.5%). ADF (42.4%) and NDF (58.3%) contents for no fertilizer treatment were significantly (p<0.05) higher than those of other treatments. However, TDN contents were higher for livestock manure treatments than for no and/or chemical fertilizer treatments. In particular, TDN contents of treatments applied 150% composted cattle manure and 150% cattle slurry showed 72.3 and 70.8%, respectively and both treatments were significantly (p<0.05) higher than all of the other treatments. For the sorghum$\times$sorghum hybrid, yields of annual DM and TDN for 100% (12.4 and 7.4 ton/ha) and 150% (13.1 and 7.6 ton/ha) cattle slurry-applied treatments, and N+P+K-applied treatments (12.6 and 7.7 ton/ha) were significantly (p<0.05) higher than those of the others. In the others, 150% composted cattle manure (9.3 and 5.2 ton/ha) had higher annual DM and TDN yields than P+K-applied (8.4 and 4.8 ton/ha) and 100% composted cattle manure treatments (7.4 and 4.2 ton/ha), with no significant difference. Crude protein contents for sorghum$\times$sorghum hybrid applied P+K and cattle slurry were 8.8 and 8.6%, respectively. CP contents for both treatments were significantly higher than those of composted manure ($7.5{\sim}8.3%$) and no fertilizer (4.0%) treatments, but 100% livestock manure treatments had higher CP contents than 150%-applied treatments. ADF and NDF contents for N+P+K and cattle slurry-applied treatments were significantly (p<0.05) lower than the others. However, TDN contents were highest in N+P+K and cattle slurry-applied treatments, showing 61.2 and 58.3 to 59.4%, respectively. These results indicated that application of livestock manure instead of chemical fertilizer to the soil of forage crops might not only improve yields of DM and TDN, but also reduce environmental pollution by producing organic roughages through recycling of livestock manure.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.2 no.1
• /
• pp.41-49
• /
• 1994

This experiment was carried out in order to study the effect of organic fertilizer on the growth of Korean lawngrass (Zoysia matrella L. Merr.) and the change of soil chemical characteristics. The results obtained are summarized as follows : 1. Organic fertilizer was appropriate for base-dressing in the sod establishment of Korean lawngrass(Z. matrella L. Merr.) 2. Chlorophyll contents in mixed application plot of complex fertilizer(21-17-17) and organic fertilizer were more abundant than that in single application plot of organic fertilizer. 3. Application of organic fertilizer promotes the contents of available phosphorous in soil. 4. Dry weights of base-dressing and top-dressing plot had more weight about 40%, 12% than that of control plot, respectively. So base-dressing was more effective than top-dressing in the application of organic fertilizer.

• Korean Journal of Soil Science and Fertilizer
• /
• v.37 no.2
• /
• pp.116-123
• /
• 2004

This study was conducted to investigate the changes in physicochemical and microbiological properties during fermenting process of organic fertilizer which was made from the mixture of organic materials such as sesame oil cake, fish meal, blood meal, rice bran, ground bone meal, and natural minerals such as illite, crusted oyster shell and loess. They were mixed and fermented for 70 days. The sesame oil cake and rice bran, major ingredients for organic fertilizers, consisted of 7.6 and 2.6% total nitrogen, 3.6 and 4.6% $P_2O_5$, 1.4 and 2.2% $K_2O$, respectively. The ground bone meal included 29.2% $P_2O_5$ and illite included 3.8% $K_2O$. Temperature of organic fertilizer during the fermentation rapidly increased over $50^{\circ}C$ within 2 days after mixing and stabilized similar to outdoor temperature after 40 days. Moisture content decreased from 36.3 to 16.0% after 1 month. C/N ratio of organic fertilizer slightly increased until 30 days and thereafter, it slowly decreased, It resulted from the faster decrease of total nitrogen concentration compared with organic matter. Concentration of $NH_4-N$ in organic fertilizer rapidly increased from 1,504 to $5,530mg\;kg^{-1}$, the highest concentration after 10 days. Meantime, $NO_3-N$ concentration was low and constant about $150mg\;kg^{-1}$ over the whole fermenting period. This result seemed to be due to the high pH. The organic ferfilizer fermented for 70 days was composed of 2.7% N, 2.8% $P_2O_5$, 1.8% $K_2O$, and 35.9% organic matter. Total populations of aerobic bacteria, Bacillus sp. and actinomycetes, after fermenting process, were $12.5{\times}10^{10}$, $45.5{\times}10^{5}$ and $13.6{\times}10^{5}cfu\;g^{-1}$ respectively. Pseudomonas sp. was $71.9{\times}10^{7}cfu\;g^{-1}$ at first, but it rapidly decreased according to the rise of temperature. Yeasts played an important role in the early stage of fermentation and molds did in the late stage.

• Applied Biological Chemistry
• /
• v.19 no.3
• /
• pp.172-183
• /
• 1976

RNA, DNA and other phosphorus fractions were determined in the leaf and root of soybean plants different in phosphorus sensitivity grown in $NH_4-N,\;NO_3-N$ and urea medium. The phosphorus sensitive cultivars contained higher ASIP (acid soluble inorganic phosphorus) than the tolerant cultivars with all nitrogen sources. ASIP was highest in the urea treated plants and lowest in the nitrate treated plants. Total phosphorus content was mostly affected with increase in ASIP. When ASIP increased, acid solsuble organic phosphorus(ASOP), phospholipids (L-P), RNA-P, residual phosphorus (R-P) tended to increase, while DNA-P showed little change. The percent RNA-P or DNA-P of total phosphorus in the nitrate treated plant was twice that in the ammonium treated plant, which were also higher in tolerant cultivars regardless of nitrogen sources. The percent ASOP in total acid soluble phosphorus $(ASOP/ASP{\times}100)$ decreased as phosphorus sensitivity decreased. Indications are that phosphorus sensitivity depends on the relative sizes of phosphorus metabolic pools. Total dry matter yield was negatively correlated with total phosphorus (r=0.84 significant at 0.01P), ASIP (0.84 significant at 0.01P) and residual phosphorus (0.69 significant at 0.05P). ASOP showed positive correlation with L-P, RNA-P and DNA-P but negative with R-P. RNA-P was significanly correlated only with L-P (0.63 at P=0.01). There was significant interaction (0.01) among nitrogen sources, cultivars and phosphorus metabolic pools. Phosphorus sensitivity and ammonium toxicity appear to be same in view of energy metabolism, that is, the former inhibits the conversion of ATP to ADP (energy releasing) through phosphate potential while the latter inhibits ATP formation (energy storing).

• Korean Journal of Soil Science and Fertilizer
• /
• v.42 no.1
• /
• pp.1-13
• /
• 2009

Understanding the net nutrient balance in a farming system is crucial in assessing the system's sustainability. We quantified N, P and K balances under vegetable organic farming in a Eutric Haplud and in West Java, Indonesia in five planting seasons from 2005 to 2007. The ten treatments and three replications, arranged in a completely randomized block design, included single or combined sources of organic fertilizers: barnyard manure, compos ts or green manures. The organic matter rates were adjusted every planting season depending on the previous crop responses. The result sshowed that the application of ${\geq}20$ t $ha^{-1}$ barnyard manure per crop resulted in positive balances of N, P, and K, except in the second crops of 2006 where potassium balance were -25 to -11 kg $ha^{-1}$ under the treatments involving cattle barnyard manure, because of low K content of these treatments and high K uptake by Chinese cabbage. Application of 20 to 25 t $ha^{-1}$ of plant residue or 5 t $ha^{-1}$ of Tithonia compost also resulted in a negative K balance. Soil available P increased significantly under ${\geq}25$ t $ha^{-1}$ barnyard manure and that under chicken manure had the highest available P. Accordingly, chicken barnyard manure gave the highest crop yield because of relatively higher N, P, and K contents. Plant residues gave the lowest yield due to the lowest nutrient content among all sources. Reducing the use of barnyard manure to 12.5 t $ha^{-1}$ and substituting it with Tithonia compost, Tithonia green manure or vegetable plant residue compost gave insignificantly different yield compared to the application of 25 t $ha^{-1}$ barnyard manure singly. In the long run, application of 25 t ha-1 cattle, goat, and horse manure or about 20 t $ha^{-1}$ chicken manure is recommendable for sustaining the fertility of this Andisol for vegetable production.