• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.1
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• pp.18-25
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• 2017

This study describes the preliminary evaluation of antioxidant and anti-inflammatory activities of Allium hookeri. A. hookeri was extracted using crude extract and then fractionated sequentially with n-hexane, $CH_2Cl_2$, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, we first examined the inhibitory effect of A. hookeri extracts on production of oxidant stresses (2,2-diphenyl-1-picrylhydrazyl, xanthine oxidase, and superoxide). In addition, we examined the inhibitory effects of A. hookeri on production of pro-inflammatory factors (nitric oxide, prostaglandin $E_2$, inducible nitric oxide synthase, and cyclooxygenase-2) in murine macrophage RAW 264.7 cells stimulated with lipopolysaccharide. Of the sequential solvent fractions of A. hookeri, EtOAc fractions showed decreased production of oxidant stresses, and $CH_2Cl_2$ and EtOAc fractions of A. hookeri inhibited production of pro-inflammatory factors. EtOAc fraction inhibited production of pro-inflammatory cytokines (interleukin-6 and -$1{\beta}$). These results suggest that A. hookeri has significant effects on oxidant stresses and pro-inflammatory factors and is a possible antioxidant and anti-inflammatory therapeutic and preventive material.

• Applied Biological Chemistry
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• v.50 no.2
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• pp.132-135
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• 2007

The effects of rose flower extracts on the growth of CHO cells were examined. Rose flower extracts were prepared by solvent extraction with hexane, ethylacetate and ether from five domestic rose cultivar, Rosa hybrida L. cv. Mihyang, Noeul, Redqueen, Whitelady and Pinklady, respectively. The effects of rose flower extracts on the growth of CHO cells were measured using MTT colormeteric assay and compared with control. Extracts of rose flowers showed stimulative effect or inhibitory effect on the growth of CHO cells depending on the kinds of solvent and concentration of extracts. Ether extracts of rose flower showed a more effective stimulative effect on the growth of CHO cells at the concentration of 5 ${\mu}g{\cdot}ml^{-1}$. These results suggest that rose flower has the simulating activity on the growth of CHO cells and a potential as new functional food source.

• KSBB Journal
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• v.23 no.2
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• pp.164-168
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• 2008

The purpose of this study was to confirm the content of total polyphenol, antioxidative and immune activities of the extracts of Cedrela sinensis leaf. The content of total polyphenol of water extracts ranged from 46.5-59.6 mg/100 g, which was higher than other extracts using organic solvents such as EtOAc, $CH_2Cl_2$ and $C_6H_{14}$. The antioxidant activity of the water and organic solvents extracts showed 6-33% in terms of 2,2-diphenyl-picryl-hydrazyl (DPPH) scavenging activity. To analyze the immuno-stimulation activity of C. sinensis leaf extract, we investigated the effect of the extracts on NO synthesis which is important in host defense against bacterial infection. Hot water extracts significantly increased NO generation by RAW 264.7, macrophage cell line, while organic solvent extract has no significant effect on NO production. To further analyzed the anti-inflammatory effect of the extracts, we investigated the effects of the extracts on lipopolysaccharide(LPS)-induced NO generation. Organic solvent extracts of C. sinensis leaves showed strong inhibitory effect on NO production in LPS-stimulated RAW 264.7 cells. These results suggest that C. sinensis leaf extract may represent a useful immune stimulating agent and anti-inflammatory agent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1204-1209
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• 2001

As a basic study to find bioactive principles for the hypolipidemic effect from natural sources, various biological influences of MeOH extracts of Thuja accidentalis L. and Juniperus chinensis var. horizontalis Nakai on rats were examined. The concentrates obtained from the organic layer and aqueous layer fractions of each species were orally administered to rats and then their effect on the body weight, organ weight, serum lipids profile, and blood glucose concentration were examined. Among the samples tested, the organic layer of Juniperus chinensis var. horizontalis exhibited the possibility of the hypolipidemic effects. The corresponding layer was chromatographed to give three fractions and reexamined their effects on rats. The results demonstrated that the fraction corresponding for the R$_{f}$ 0.4~0.7 which was the mixture of many minor components showed the effect of lowering the blood-lipid bevel effectively without any side effects. Besides, communic acid, identified from and isolated as a major component of the species exhibited the possibe hypolipidemic effects.s.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.69-69
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• 2012

지구상에는 약 40만 종의 식물이 존재하는 것으로 알려져 있으며 이 중에 는 질병의 치료를 위한 약재로 이용되거나 나무는 대부분 목재 등으로 이용된다. 향나무(Juniperus chinenesis L.)는 항균성 및 방충성이 뛰어난 상록수로서 목재는 조각재나 가구재로 사용되고 민간 및 한의학에서 다양한 증상의 약재로 쓰이고 있으며 부패한 냄새를 제거할 목적이나 향재 및 향료로도 쓰이고 있다. 열매의 정유성분은 화장품, 술, 캔디 등에 사용되며 cedrol은 향료보류제, 유분은 훈향료, 목부는 고혈압, 곽란, 심복통, 통기파혈에 쓰인다. 향나무는 폴레페놀 성분이 함유되어 있고 피톤치드가 많이 나오는 것으로 알려져 있으며 심재의 폴리페놀 화합물은 항암, 항균, 항알러지, 노화방지 및 심장질환 등을 예방하거나 지연시키는 등 광범위한 약리학적 활성을 나타내어 식품, 의약품, 화장품, 향료등 다양한 분야에 활용되고 있다. 향나무 추출물에 관한 연구로는 성분분석과 생리활성 등에 관한 의약학 분야의 다양한 연구와 항산화, 항균활성 등 약리작용에 연구가 최근 다양하게 이루어지고 있는 것을 볼 수 있다. 측백나무속에 속하는 나무들은 대부분 정유 성분을 함유하고 있는 특성으로 항균성 등 바이오 기능성 소재 개발을 위한 연구가 활발히 진행되고 있으나, 향나무 추출물의 염색성에 관한 연구는 찾아보기 어려운 실정이다. 이에 본 연구에서는 다양한 용매를 이용하여 향나무의 잎, 열매, 수피, 심재 각 부위 추출물을 이용하여 견직물에 대한 염색성을 살펴봄으로써 향나무 추출물을 활용한 천연염색과 염재로서의 가능성을 확인하고자 하였다. 실험 결과 증류수를 염액 용매로 염색한 결과 모든 추출물의 잎, 열매 염색포의 색상은 대부분 옅은 Y계열로 나타났으며 향나무의 수피와 심재 염색포는 대부분 적색기미가 강한 YR계열로 나타났다. 에탄올 혼합액을 염액 용매로 하여 염색한 결과 향나무의 잎과 열매의 색상은 대부분 Y계열의 색상을 나타냈고 유기용매 추출염색포에서 정유성분으로 추정되는 물질 때문에 균염이 어려웠으며 수피와 심재 염색포에서 대부분 R계열의 색상이 뚜렷이 나타났다.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.111-114
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• 2004

This study was carried out to evaluate the inhibition of growth and collagenase activity of Salvia miltiorrhiza Bunge against microorganisms causing periodontal diseases. The ethanol extract of Salvia miltiorrhiza showed sig-nificant growth inhibition against microorganisms causing periodontal diseases. Ethanol extract was further fractionated with organic solvents in the order of hexane, chloroform and ethyl acetate. Among the fractions tested, the hexane fraction showed the highest cell growth inhibition. The minimal inhibitory concentration (MIC) of the extract against C. curvus, C. rectus, E. corrodens, F nucleatum, P. gingivalis, P. intermedia and W. succinogenes were 200, 50, 50, 250, 150, 250 and 200 ${\mu}g$/ml, respectively. The inhibition of collagenase activity by organic solvent fractions were higher than that of minocycline, and the inhibition ratio of collagenase activity was $88.2{\pm}2.1$ % in the chloroform fraction.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.4 no.2
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• pp.114-119
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• 2003

This study is to remove several organic pollutants from wastewater by non-dispersive membrane solvent extraction technique. The distribution coefficients of several solvents were determined and the experimental system was operated counter-currently and cocurrently with respect to the aqueous phase and solvent. In these experiments, as the flow rate of aqueous solution inclosed, due to being shortened contact time to solvent, the rate of removal of organic pollutants decreased and as the flow rate of solvent increased, the rate of removal increased. Meanwhile, the rate of removal of organic pollutants for the countercurrent flow system was higher than that for the occurrent flow system.

• Clean Technology
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• v.29 no.2
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• pp.109-117
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• 2023

The purpose of this study is to develop a method for separating antioxidants and sugars from grape skin extract. The extract was first mixed with a variety of organic solvents to investigate whether the separation was feasible. When employing acetone, ethanol, dimethylsulfoxide, or dimethylformamide, the organic solvent-extract combination formed a single phase. However, when benzene, ethyl acetate, or n-hexane was added to the extract, the mixture separated into an organic and an aqueous phase and the pigments remained in the aqueous phase. On the other hand, when polyethylene glycol-2,000 (PEG-2000) and sodium citrate were added to the extract, the mixture was separated into three layers, with the majority of the flavonoids migrating to the top layer and 53% of the extract's glucose migrating to the bottom layer. The top layer had significant antioxidant activity, whereas the bottom layer showed no antioxidant activity. The glucose recovery in the bottom layer increased as the molecular weight of PEG increased and the highest recovery (67%) was observed when PEG-8,000 was added. The highest flavonoid separation was observed with PEG-2,000, followed by PEG-8,000 and PEG-400. The flavonoid separation when PEG-2,000 was added resulted in a flavonoid recovery of 48% and 0.2% from the top and bottom layers, respectively. Examining the effect of the separated solution using the agar disc diffusion method on yeast cell growth confirmed that the addition of the extract, the top, and the bottom layer did not inhibit cell growth.

• Journal of Mushroom
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• v.17 no.4
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• pp.241-246
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• 2019

Samples (10 mg/mL) of wild Grifola frondosa aqueous extract and solvent fractions were examined for fibrinolytic, thrombin inhibitory, acetylcholinesterase inhibitory, and antioxidative activities to determine the biological activities. The fibrinolytic activity of the aqueous extract and solvent fractions was 0.93 and 0.73 plasmin units/mL, respectively. The thrombin inhibitory activity of the butanol extract was 79.60%. The chloroform fraction had high acetylcholinesterase inhibitory activity (85.88%). The aqueous extract had low antioxidative activity (39.81%). The aqueous fraction hydrolyzed Bβ subunits of human fibrinogen but did not show any reactivity for the γ form of the human fibrinogen. The findings indicate the potential of wild Grifola frondosa for the development of drugs and bio-functional foods to prevent cardiovascular diseases.

• The Korean Journal of Food And Nutrition
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• v.17 no.4
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• pp.368-373
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• 2004

Optimal extraction conditions were established from the difference of quality characteristics according to extraction conditions of Ssangwha extracts(SWE). Extract yields of SWE obtained from the established extraction conditions were as follows. The maximum yield was 48.90% at extraction temperature 90$^{\circ}C$ and alcohol concentration 50%, extraction yield and alcohol concentration of extraction solvent was proportioned. Increase of extraction yields at extraction temperature 80∼90$^{\circ}C$ and more than alcohol concentration 30% had slowdown tendency according to increase of alcohol concentration. At this view point, the optimum extraction conditions were alcohol concentration 30% and more than extraction temperature 80$^{\circ}C$. In this study, optimal extraction conditions of SWE were extraction temperature 90$^{\circ}C$ and alcohol concentration 30%.