• 농업기술회보
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• v.44 no.2 s.509
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• pp.60-60
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• 2007

• Journal of environmental and Sanitary engineering
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• v.16 no.3
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• pp.22-27
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• 2001

It have been investigated how algal and bacterial symbiotic reaction influences on removal of organic carbon in river ecosystem. And artificial experimentation apparatus was made for algae'and bacteia'culture as lab scale. Investigating and researching minutely the change of concentration of organic carbon substrate and the change of population density of algae'and of bacteria'with this artificial experimentation apparatus, the next results could be obtained. 1. Successful decrease of DOC(dissolved organic carbon) could not be expected unless algal and bacterial biomass floe was nut formed effectively and unless biosorption was not proceeded effectively in the very culture system in which artificial synthetic wastewater was supplied continuously at constant rate. 2. In conditions of culture liquid of 1335 glucnse mg/L(type 1) and of 267 glucose mg:L(type 2), the algal dominant species was always Chlorella vulgaris in both types in which artificial synthetic wastewater were supplied continuously at constant rate and algae population density was around maximum 107 cells/mL. 3. It was around 108 ~ 107 cells/mL that the population density of heterotrophic bacterium. In culture medium systems type 1 and type 2 in which artificial wastewater were supplied continuously at constant rate, the same density appeared initially when using the population density of Escherichia coli w 3110 as indirect indicator. And this density decreased rapidly till the culturing date 35 days were passed away, while this density increased with gentle slope after same date and then the trend of change at type 2 was more severe than one at type 1. 4. When seeing such a change of population density of Escherichia coli w 3110, the growth of heterotrophic bacterium appeared as survival instinct pattern of broader requirement of nutrient at condition of low concentration of organic carbon substrate than condition of high concentration of same substrate.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.23 no.2
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• pp.91-108
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• 2018

An ecological study on subtidal macrobenthic fauna was conducted from 25 stations in the estuarine area of Wando-Doam Bay, southern coast of Korea during August 2013. A total of 186 species was collected with a mean density of $1,229ind./m^2$ and a mean biomass of $265.7g/m^2$. Polychaetes showed the richest benthic fauna comprising 43% of total fauna, whereas mollusks appeared as density- and biomass-dominant fauna accounted for 45% and 48% of the mean density and biomass, respectively. The number of species and mean faunal density were relatively higher at the stations surrounded by Sinjido, Joyakdo and Gogeumdo showing a gradual decrease toward inner bay stations. Species number and density were negatively correlated with bottom water temperature, but they were positively correlated with both the bottom salinity and DO. The most dominant species in terms of density was a semelid bivalve, Theora fragilis which showed a positive correlation with TOC content of surface sediment and its high density occurred around Gogeum-Sinji-Joyakdo area where dense aquaculture facilities exist. In the bay mouth area, an amphipod species, Eriopisella sechellensis showed its higher density at the stations with low organic content but fine grains. The combination of water temperature, salinity, pH of bottom water, water and sulfur content of the surface sediment could explain 71% of the spatial distribution of macrobenthic fauna from the Bio-Env analysis. From the cluster analysis, the study area consisted of 6 distinct station groups lineated from offshore area toward inner area. Ampharete arctica, Goniada maculata, Eriopisella sechellensis, Theora fragilis, Caprella sp. were identified as the main contributing faunas in classification by the SIMPER analysis. From the value of BPI, the benthic communities at the inner and central Wando-Doam Bay were assessed to be in a normal condition whereas those at the outer Wando harbor and Gogeum-Sinji-Joyakdo area were assessed in a poor or very poor condition due to the high concentration of particulate organic matter might be originated from the nearby dense aquaculture facilities. This study indicated that pristine inner bay has been influenced by the organic material supplied from the outer bay. Thus it is necessary to establish an ecological management plan to reduce organic enrichment of sediment from dense aquaculture facilities in the outer bay.

• Korean Journal of Ecology and Environment
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• v.33 no.4 s.92
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• pp.380-386
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• 2000

Alkaline phosphatase ($AP_{ase}$) activity was studied for the pure cultures and natural communities of phytoplankton. The Michaelis-Menten constant ($K_m$) showed large variation with species. Some green algae showed large $K_m$ ($650\;{\mu}M$ for Selenastrum capriconutum). Chlorella sp. and Nitzschia palea showed smaller Km (respectively1.7, 2.0 HM) than those of other species examined. The extracellular free enzyme in the filtrate of Anabaena flos-aquae showed smaller Km ($52\;{\mu}M$) than that of cellbound form ($276\;{\mu}M$). The $K_m$ ($12.0\;{\mu}M$) of summer phytoplankton in Lake Soyang. when Anabaena sp. was dominant species, was larger than that (1.5 HM) of spring phytoplankton when Asterionella sp. was the dominant. Although maximum activity($V_{max}$) in Lake Soyang was affected by the concentration of DIP within the lake, but the $V_{max}$ always varied not with the DIP concentration of the lake. Induction of $AP_{ase}$ may be more affected by the phosphate content within the cell of phytoplankton than by the concentration of DIP within the lake, The extracellular free $AP_{ase}$ activity accounted for $36{\sim}97%$ of total activity from fall to spring turnover in Lake Soyang. The $K_m$ ($1.1{\mu}3.5\;M$) of extracelluar free enzyme were simillar to those ($0.7{\mu}3.5\;M$) of the total activity. This indicates that the extracelluar free enzyme was derived from phytoplankton.

• Ecology and Resilient Infrastructure
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• v.11 no.2
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• pp.23-34
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• 2024

Sediment, aquifer materials, surface water, and groundwater from brackish Songji lake affected by salinity of seawater, were collected and a pilot scale column experiment was conducted to simulate the nitrogen transport through the hyporheic zone. Upstream experiments of groundwater displayed that groundwater containing a small amount of salt percolated into aquifers and sediments, maintaining low dissolved oxygen concentrations. In addition, partial denitrification occurred in the aquifer due to salinity and low dissolved oxygen, resulting in the accumulation of NO₂^-. In sediments,nitrogenous compounds were reduced due to adsorption by long residence times or microbial-mediated oxidation/reduction reactions. Downstream experiments of surface water displayed that surface water from the brackish lake, containing high concentrations of dissolved oxygen and salts, infiltrated into the sediments and aquifer, supplying high dissolved oxygen concentrations. This resulted in biological nitrification in the sediments and aquifer, which reduced nitrogen-based pollutants despite the high salt concentration in the surface water. Whereas partial denitrification at low dissolved oxygen concentrations in the upwelling mixing zone was observed by salinity and accumulated NO₂^-, nitrification at high dissolved oxygen concentrations in the downwelling mixing zone was not significantly affected by salinity. These results confirm that salinity in the brackish water lake has some influence on the nitrogen behavior of the hyporheic mixing zone, although nitrogen behavior is a complex combination of factors such as DO, pH, substrate concentration, and organic matter concentration.

• Korean Journal of Microbiology
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• v.51 no.2
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• pp.97-107
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• 2015

Wetlands constitute a transitional zone between terrestrial and aquatic ecosystems and have unique characteristics such as frequent inundation, inflow of nutrients from terrestrial ecosystems, presence of plants adapted to grow in water, and soil that is occasionally oxygen deficient due to saturation. These characteristics and the presence of vegetation determine physical and chemical properties that affect decomposition rates of organic matter (OM). Decomposition of OM is associated with activities of various extracellular enzymes (EE) produced by bacteria and fungi. Extracellular enzymes convert macromolecules to simple compounds such as labile organic carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) that can be easily taken up by microbes and plants. Therefore, the enzymatic approach is helpful to understand the decomposition rates of OM and nutrient cycling in wetland soils. This paper reviews the physical and biogeochemical factors that regulate extracellular enzyme activities (EEa) in wetland soils, including those of ${\beta}$-glucosidase, ${\beta}$-N-acetylglucosaminidase, phosphatase, arylsulfatase, and phenol oxidase that decompose organic matter and release C, N, P, and S nutrients for microbial and plant growths. Effects of pH, water table, and particle size of OM on EEa were not significantly different among sites, whereas the influence of temperature on EEa varied depending on microbial acclimation to extreme temperatures. Addition of C, N, or P affected EEa differently depending on the nutrient state, C:N ratio, limiting factors, and types of enzymes of wetland soils. Substrate quality influenced EEa more significantly than did other factors. Also, drainage of wetland and increased temperature due to global climate change can stimulate phenol oxidase activity, and anthropogenic N deposition can enhance the hydrolytic EEa; these effects increase OM decomposition rates and emissions of $CO_2$ and $CH_4$ from wetland systems. The researches on the relationship between microbial structures and EE functions, and environmental factors controlling EEa can be helpful to manipulate wetland ecosystems for treating pollutants and to monitor wetland ecosystem services.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.21 no.1
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• pp.11-23
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• 2016

An ecological study on subtidal macrobenthic fauna was conducted off the Namhaedo Island, south coast of Korea from July 2012 to April 2013. A total of 247 species of macrobenthos occurred with a mean density of $1,027ind./m^2$ and a mean biomass of $148.7g/m^2$. Polychaetes was the richest and most abundant faunal group that comprised 37% in both total species number and density whereas echinoderms were biomass-dominant faunal group that accounted for 44% of the mean biomass. There was a seasonal variation in the species richness and abundance of macrobenthos with more species in winter and higher density in spring. Mean faunal density was relatively high at the stations near Namhaedo Island, but gradually decreased toward offshore stations. The most dominant species in terms of density was an amphipod species, Eriopisella sechellensis which occurred as a top ranker during three seasons except spring recorded the fourth rank. E. sechellensis showed its high density at the near shore stations of Namhaedo Island, but this species did not occur around the entrance of Gwangyang and Saryang Bays where Theora fragilis and Lumbrineris longifolia showed high densities. In particular, Tharyx sp., recorded high density between Gwangyang Bay entrance and offshore after Sea Prince oil spill, did not occur in the same area during this study period. The bottom temperature and sorting value of the surface sediments were highly correlated to the spatial distribution of macrobenthic fauna from the Bio-Env analysis. From the cluster analysis, the study area has five station groups with more similar faunal affinities from inner area toward offshore area. Based on the SIMPER analysis T. fragilis, Magelona japonica, E. sechellensis, L. longifolia and Paraprionospio cordifolia were mainly contributed to the classification of station groups. From the BPI, benthic communities in the entrance of Gwangyang Bay and Saryang Bay were considered to be in a slightly polluted condition in contrast to the normal healthy community at the offshore of Namhaedo Island. These results suggested that the benthic community of this area should be regularly monitored to assess the health status of this benthic ecosystem.