• Proceedings of the Korean Institute of Communication Sciences Conference
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• 1995.06a
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• pp.678-681
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• 1995

• Korean Journal of Agricultural Science
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• v.7 no.2
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• pp.59-64
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• 1980

These experiments were carried out to define the effects of 2.4-D, NAA and Benzyladenine on the differentiation and growth of the organs and the induction of callus from the potato meristem. The results were summarized as follows ; 1. The differentiation and growth of the shoots from the potato meristem was promoted in increased concentration of Benzyladenine but the callus was not induced on the M.S. medium containing Benzyladenine. 2. On the M.S. medium containing NAA 0.5 mg/l or higher concentration of NAA, the shoots were not initiated but the callus were induced from potato meristem. The growth of callus was promoted in increased concentration of NAA. 3. The roots were initiated from 50% of potato meristems planted on the M.S. medium containing more than 0.1 mg/l of NAA but the roots were pot initiated on the medium containing 2.4-D. 4. The shoot growth was significantly increased by increasing of 2.4-D concentration up to 0.1 mg/l, but the shoots were not initiated on the medium containing 2.4-D more than 1.0 mg/l. 5. For the induction and growth of the callus from potato meristem, NAA was more effective than 2.4-D and the most effective medium was M.S. medium supplemented with 2.0 mg/l of NAA. 6. The M.S. mediums supplemented with BA 2.0 mg/l and NAA 0.1 mg/l or BA 1.0 mg/l and 2.4-D 0.1 mg/l showed good results for entire plant regeneration from potato meristem.

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.35-39
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• 1995

In order to investigate the effects of gelling agent on rice anther culture, anthers of rice (Japonica cv Daecheongbyeo) were cultured on N$_{6}$ media supplemented with 0.8, 1.2 or 1.6% Junsei agar and 05, 0.4, 0.6, 0.8 or 1.0% Gelrite (Phytagel, Sigma). On Junsei agar media, the frequency of callus induction was decreased in proportion to agar concentration. The frequency of callus induction was more increased as 67.6% and 54.8% in media containing 0.4 and 0.6% Gelrite than in agar media. The frequency of plant regeneration and spontaneous doubled-diploid was directly proportional to Junsei agar and Gelrite concentration. The number of green and spontaneous doubled diploid plant was highest on 0.6% Gelrite medium. In order to optimize the concentration of growth regulators for the callus induction medium containing 0.6% Gelrite, anthers were cultured on N$_{6}$ media supplemented with 2mg/L NAA, 2 mg/L 2,4-D, 1mg/L NAA and 1mg/L 2, 4-D, or 1mg/L NAA, 1mg/L 2,4-D and 0.5mg/L kinetin. The maximum frequency of callus induction and plant regeneration was obtained from the medium supplemented with 2 mg/L NAA and 0.6% Gelrite. In conclusion the induction of embryogenic callus, the frequency of plant regeneration and in vivo chromosome doubling was more effective in Gelrite media than in Junsei agar media.dia.

• Korean Journal of Organic Agriculture
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• v.26 no.2
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• pp.233-243
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• 2018

This study was conducted to elucidate the effects of green manure crop on growth characteristics and saponin contents of Platycodin grandiflorum Ridix roots for organic farming. Rye, hairy vetch and rye+hairy vetch were cultivated as green manure crops with several different methods before transplanting P. grandiflorum Radix. In root width and number of fine-roots, when the highest scores (23.9 mm, 25.7) was recorded in rye pre-cultibated plot in $2^{nd}$ year cultivation. Fresh weight of the Platycodin grandiflorum Ridix root was no significant difference among the different green manure crop treatments, but fresh weights of the Platycodin grandiflorum Ridix root of all green manure crop treatments were higher than that of control. The total content of saponin was highest detected in Platycodin grandiflorum Ridix roots of the hairy vetch plot (1,106 mg/100 g) and rye plot (1,693 mg/100 g) in $1^{st}$ and $2^{nd}$ year cultivation, respectively. Therefore, we confirmed that precultivation of the green manure crops showed significant differences in the growth characteristics and saponin contents of the Platycodin grandiflorum Ridix root, and growth and saponin contents in the Platycodin grandiflorum Ridix root of rye precultivated plot was much higher in $2^{nd}$ year cultivation than $1^{st}$ year cultivation.

• Korean Journal of Organic Agriculture
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• v.17 no.1
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• pp.55-62
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• 2009

In this paper, we assessed the growth of rice seedlings(Chucheong variety) in commercial organic growth medium that was substituted with different ratios of vermicast of oyster mushroom waste grown under potting alone, and potting and floor layering treatment. The commercial organic growth medium was substituted with vermicast at ratios of control, 2%, 4% and 6%, respectively. The control consisted of commercial organic growth medium alone without vermicast. Incorporation of $4%{\sim}6%$ vermicast of oyster mushroom waste into a commercial organic growth medium enhanced the growth of rice seedlings significantly as compared to commercial organic growth medium alone(control), and 2% amended with vermicast in potting treatment. This results demonstrate that substitution with low ratios of vermicast($4%{\sim}6%$) will promote growth of rice seedlings. The growth of rice seedlings in commercial organic growth medium alone without vermicast was enhanced significantly as compared to the substituted with vermicast in floor layering treatment, it may due to nutrient uptake by elongated root from the vermicast when applied to on the floor layering. Floor layering treatment is an effective method for potting processing of vermicast. The vermicast of oyster mushroom waste should have a high safety and great potential as materials of growth media for increasing plant growth, either as soil conditioner, or as substitution or amendments to commercial organic growth medium. For the enhanced growth of rice seedlings, demand to increase with total nitrogen, and decrease with the carbon and nitrogen ratio(C/N) of commercial organic growth medium supplied by such as vermicast.

• Journal of Korean Society of Forest Science
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• v.78 no.4
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• pp.412-418
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• 1989

The stem segments of Ailanthus altissima were cultured on the Murashige & Skoog's medium(1962) supplemented with 0.1 mg/l BAP and 1.0 mg/l 2, 4-D for callus induction and proliferation, Shoot primordia were observed as greenish regions on the surface of yellow-brown calli about 8 weeks after culture. Shoot primordia were selected and transferred to the MS media containing various combination of BAP and 2, 4-D. Among these combinations the shoot primordia cell clusters on the medium added to 0.5mg/l BAP and 0.01mg/l 2, 4-D exhibited the highest number of shoot formation. These shoots were successfully transferred on the solid MS medium with no growth regulators for the rootings.

• Journal of Plant Biotechnology
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• v.39 no.1
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• pp.69-74
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• 2012

Using calli of $Muscari$ $armeniacum$ cv. 'Early Giant' that is monocotyledonous ornamental bulb crop with increasing demand in Korea, we carried out current studies to establish an in vitro multiple propagation protocol via somatic embryogenesis. We found that soft pale yellow green calli were induced from leaf explants cultured on all media containing 0.1~3.0 $mg{\cdot}L^{-1}$ auxins such as 1-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). However, induced calli showed vigorous growth only when they further transferred on same media containing 2,4-D, 4-amino-3,5,6-tri-chloropicolinic acid (picloram), or 3,6-dichloro-o-anisic acid (dicamba). Although frequency of somatic embryo induction depended on callus source and PGR composition in somatic embryo induction media, somatic embryogenesis was initiated on surface of proliferated calli after transferring on media with no PGR or 0.01 $mg{\cdot}L^{-1}$ NAA co-supplemented with various cytokinins such as $N^6$-benzylaminopurine (BAP). Highest number of embryo at 9.3 per callus clump was obtained when calli which were grown under 0.1 $mg{\cdot}L^{-1}$ picloram supplementation were sub-cultured on medium with 0.01 $mg{\cdot}L^{-1}$ NAA and 0.5 $mg{\cdot}L^{-1}$ BAP. In addition, morphological characteristics of somatic embryo were categorized into following nine phases: globular, biased heart, biased torpedo, early cotyledonary, middle cotyledonary, late cotyledonary, early sprouting, middle sprouting, and late sprouting embryos.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.368-371
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• 2000

Pantoea agglomerans의 인산 가용화 정도를 조사하기 위하여 합성배지인 HY와 G broth에서 실험을 수행한 결과 pH와 glucose의 감소 정도에 따른 인산의 가용화 정도는 역상 관계임을 알 수 있었다. aeration조건에서 240시간대에 pH 3.48, glucose양 61245ppm일 때 인산의 가용화 정도가 1675ppm으로 가장 많이 나타났으며, 이때 균은 모두 사멸하였다. 따라서 균이 glucose인 탄소원을 기질로 이용하면서 생성되는 유기산에 의해 시간이 경과함에 따라 pH와 glucose양은 감소하게 되고, 유기산에 의해 불용성 인산인 Hydroxyapatite가 가용성 인산으로 바뀌게된다.

• Microbiology and Biotechnology Letters
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• v.6 no.4
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• pp.161-165
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• 1978

Out of 11 organic acid producing strains isolated from fruits, soil, and air, one strain was selected for the study of the citric acid fermentation using Sakaguchi's medium. The organism was identified as Aspergillus niger. When Asp.niger was shaked at 3$0^{\circ}C$ in a cotton plugged 500 mι Erlenmeyer flask with 100 ml of Sakagnchi's medium containing 10% of glucose (Difco), 0.6% of peptone, and mineral, citric acid were produced at the level of 17 gram per liter in 14 days. The citric acid was also produced at the level of 35 gram per liter after the improvements of Sakaguchi's medium-the adaptation, peptone addition, aeration, methanol addition, and glucose addition.

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.99-102
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• 1998

The most effective medium for shoot initiation in vitro of peach cv. Baekmijosaeng, Yumyeong and nectarine cv. Cheonhong was Quoirin and Lepoivre medium(LP). 20 g/L and 30 g/L sorbitol as carbon source were effective for shoot proliferation of cv. Baekmijosaeng. Addition of 500 mg/L lacto albumin enzymatic hydrolysate(LH) increased shoot number per explant of cv. Baekmijosaeng peach. Removing the apical meristem and horizontal placing of explants on the medium increased cv. Baekmijosaeng shoot.