• Journal of Mushroom
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• v.12 no.2
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• pp.107-116
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• 2014

Gloeostereum incarnatum is an edible and medicinal mushroom belongs to Family Cyphellaceae of Polyporales, Basidiomycota. The purpose of this study was to investigate the free radical scavenging, anti-inflammatory, and melanin synthesis inhibitory activities of fruiting bodies of Gloeostereum incarnatum. In the free radical scavenging activities, the mushroom extracts showed good 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and chelating activity on the ferrous ions compared with the positive control, BHT. The mushroom extract suppressed nitric oxide (NO) production in LPS-induced RAW 264.7 macrophage cells in dose dependant manners. Significant reduction of paw edema of rats were observed at 2~6 h after administration with 50 mg/kg of the methanol and hot-water extracts, which were comparable with treatment of 5 mg/kg of indomethacin, the positive control. The melanin synthesis of Melanoma B16/F10 cells treated with $100{\mu}g/mL$ of the methanol and hot water extracts decreased melanin concentration to 50% and 45% compared with the control, arbutin. Therefore, the experimental results showed that methanol and hot-water extracts of Gloeostereum incarnatum fruiting bodies might be used for good sources of anti-inflammatory, free radical scavenging, and skin whitening agents for human health.

• Journal of Life Science
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• v.13 no.5
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• pp.642-650
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• 2003

To develop a new approach to the treatment of neuroblastoma cells we evaluated the effect of cAMP on the Ewing's sarcoma cell line CHP-100. We observed that the proliferation-inhibitory effect of cAMP analogs was due to cell cycle arrest and induction of apoptosis, which was confirmed by observing the morphological changes and DNA fragmentation. DNA flow cytometric analysis revealed that cAMP arrested the cell cycle progression at the G1 phase, which effects were associated with inhibition of phosphorylation of retinoblastoma protein (pRB) and enhanced binding of pRB and the transcription factor E2F-1. cAMP also suppressed the cyclin-dependent kinase (Cdk) 2 and cyclin E-associated kinase activity without changes of their expressions. Furthermore, cAMP induced the levels of Cdk inhibitor $p21^{WAF1/CIP1$ expression and p21 proteins induced by cAMP were associated with Cdk2. Overall, our results identify a combined mechanism involving the inhibition of pRB phosphorylation and induction of p21 as targets for cAMP, and this may explain some of its anti-cancer effects.

• The Journal of the Acoustical Society of Korea
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• v.20 no.3
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• pp.24-33
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• 2001

this paper describes a baseline for an implementation of a corpus-based Korean TTS system. The conventional TTS systems using small-sized speech still generate machine-like synthetic speech. To overcome this problem we introduce the corpus-based TTS system which enables to generate natural synthetic speech without prosodic modifications. The corpus should be composed of a natural prosody of source speech and multiple instances of synthesis units. To make a phone level synthesis unit, we train a speech recognizer with the target speech, and then perform an automatic phoneme segmentation. We also detect the fine pitch period using Laryngo graph signals, which is used for prosodic feature extraction. For break strength allocation, 4 levels of break indices are decided as pause length and also attached to phones to reflect prosodic variations in phrase boundaries. To predict the break strength on texts, we utilize the statistical information of POS (Part-of-Speech) sequences. The best triphone sequences are selected by Viterbi search considering the minimization of accumulative Euclidean distance of concatenating distortion. To get high quality synthesis speech applicable to commercial purpose, we introduce a domain specific database. By adding domain specific database to general domain database, we can greatly improve the quality of synthetic speech on specific domain. From the subjective evaluation, the new Korean corpus-based TTS system shows better naturalness than the conventional demisyllable-based one.

• Management & Information Systems Review
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• v.33 no.3
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• pp.35-57
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• 2014

Relationship marketing has been dealt with as an effective strategy for customer loyalty in prior research. It seems to be essential that organizations should make efforts to develop successful relationship between the organizations and the customers. However, a customers' voluntary efforts are also needed to strengthen the relationship meaningfully. Relationships are built on the foundations of mutuality. In other words, customers' efforts are necessary for relationship development, as well as an organization's efforts. Hereby, the role of customers for the development of a relationship with an organization has been overlooked in previous research so far. This study applied the Self-Determination Theory (SDT) to explain the role of customer motivation in developing customer relationship. Thus, the author chose customers who have experienced medical service recently as survey participants. Then, this paper verified the path relationships between self-determination factors (autonomy, perceived competence, relatedness) and relational factors(shared responsibility, commitment, relationship retention). It suggested that role of customers is inevitable in developing the relationship. In conclusion, this research has several implications on relationship marketing. For service providers, they should understand that the customer's perceived self-determination can improve tangible and intangible performance in relationship development.

• The Journal of the Korean Society for Microbiology
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• v.13 no.1
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• pp.55-62
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• 1978

Adult mice were injected with a single sublethal dose of cyclophosphamide. Effects of the drug on the body weight, spleen weight, and morphology of the peripheral lymphoid system have been analysed. The body weights of the mice given cyclophosphamide(300mg/kg body weight) decreased slightly and returned to normal quickly. Spleen weights, however, changed greatly by keeping the process of decrease, recovery, overshoot, and gradual return to normal only by 20 days. Histologic examinations of spleen and popliteal lymph node showed that follicles disappeared 1 to 2 days before periarteriolar lymphatic sheath or paracortex. At the peak of splenomegaly, the architectures of spleen and lymph node were replaced with the interstitial tissue composed of dense and uniform layer of lymphoid cells. With the return of spleen weight to normal range, the architecturles returned to normal. Our results clearly indicated that cyclophosphamide affected not only B cells but also T cells. These results seemed to suggest that augmentation of delayed-type hypersensitivity by cyclophosphamide may be due to the eliminateion of the suppressor T cells.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.1
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• pp.69-77
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• 2020

The tumor suppressor gene, p53, is inactivated in the human hepatocellular carcinoma cells line, Hep3B. Berberine has been reported to inhibit the proliferation of cancer cells. This study examined whether apoptosis was induced in berberine-treated Hep3B cells and observed the association between apoptosis and the expression of p53 and DNA methyltransferase (DNMT). The cell viability was measured using an MTT assay. Apoptosis of Hep3B was measured using annexin V flow cytometry. Berberine-treated cells were examined for their DNMT enzymatic activity, mRNA expression, and protein synthesis. The p53 levels were examined by Western blot analysis. The berberine treatment resulted in increased Hep3B cell death and apoptosis in a time- and dose-dependent manner. The DNMT3b activity, mRNA expression, and protein levels all decreased after the berberine treatment. In contrast, the p53 protein levels increased with a concomitant decrease in DNMT3b. No change in the expression of ERK was observed, but the P-ERK levels decreased in a dose dependent manner. These results indicate that a treatment of Hep3B cells with berberine can reduce the expression of DNMT3b, leading to an increase in the tumor suppressant gene p53 and an increase in cell apoptosis. This shows that berberine can effectively suppress the proliferation of liver cancer cells.

• Journal of Life Science
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• v.27 no.11
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• pp.1340-1348
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• 2017

Sanguinarine is a benzophenanthridine alkaloid derived from the roots of Sanguinaria canadensis L., which is used for the purpose of treating various diseases. Although studies of anticancer activities have been performed using various cancer cell lines, the phenomenon of inducing apoptosis in cancer cells by using sanguinarine requires more research. Therefore, this study investigated the anti-cancer activities and related mechanisms of sanguinarine used with Hep3B human hepatocellular carcinoma cells in terms of the regulation of apoptosis. Sanguinarine inhibited the proliferation of Hep3B cells in a concentration-dependent manner, which was associated with the induction of apoptosis. Sanguinarine also increased the activity of caspase-3, which is a typical effector caspase, and the activities of caspase-8 and caspase-9, which are key when initiating extrinsic and intrinsic apoptosis pathways, respectively. In addition, sanguinarine increased the expression of death receptor-related genes and pro-apoptotic BAX, which belongs to the Bcl-2 family, while suppressing the expression of anti-apoptotic Bcl-2. Sanguinarine promoted the truncation of Bid and enhanced the release of cytochrome c from the mitochondria to the cytoplasm due to a loss of mitochondrial membrane potential. Furthermore, the reduction of a survival rate that was induced by sanguinarine and the induction of apoptosis disappeared with the inhibition of artificial caspase activity. Therefore, the results of the study indicated that sanguinarine-induced apoptosis in Hep3B cells involves both extrinsic and intrinsic pathways; such apoptosis is a caspase-dependent phenomenon.

• Journal of the Economic Geographical Society of Korea
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• v.3 no.2
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• pp.81-96
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• 2000

The study examines the present condition and prospects of venture firm accumulation center in the case of kwan-ak venture town. The survey shows most of companies have been founded since 1997. Their major items are software development and the average employees are under 10 workers. According to the questionary about the type of R&D and the level of innovation, technology innovation such as the development of new product is advanced whereas tacit innovation like inter-firm cooperation is very weak. And the source of idea and information is concentrated on the within-firms and research center As a result of the analysis of regional linkage, the dependence of production and R & D is large on kwan-ak-gu, but sales and information services have emphasis on Seoul area. In the light of the affiliation of inter-firm, they response sympathy with cooperation, but could not strengthen their commercial ties yet. At last, the policy for venture firm accumulation center must intend to make tacit measures through inventing of system instead of the simple means such as the assistance of location, finance and tax.

• Nuclear Engineering and Technology
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• v.25 no.1
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• pp.51-62
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• 1993

A computational program ［TDET］ of the particle transport equation is developed on radiation shielding problem in two-dimensional cartesian geometry based on the discrete element method. Not like the ordinary discrete ordinates method, the quadrature set of angles is not fixed but steered by the spatially dependent angular fluxes. The angular dependence of the scattering source term in the particle transport equation is described by series expansion in spherical harmonics, and the energy dependence of the particles is considered as well. Three different benchmark tests are made for verification of TDET : For the ray effect analysis on a square absorber with a flat isotropic source, the results of TDET calculation are quite well conformed to those of MORSE-CG calculation while TDET ameliorates the ray effect more effectively than S$_{N}$ calculation. In the analysis of the streaming leakage through a narrow vacuum duct in a shield, TDET shows conspicuous and remarkable results of streaming leakage through the duct as well as MORSE-CG does, and quite better than S$_{N}$ calculation. In a realistic reactor shielding situation which treats in two cases of the isotropic scattering and of linearly anisotropic scattering with two groups of energy, TDET calculations show local ray effect between neighboring meshes compared with S$_{N}$ calculations in which the ray effect extends broadly over several meshes.eshes.

• Korean Journal of Food Science and Technology
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• v.37 no.2
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• pp.221-227
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• 2005

Apoptosis of Prunus salicina Lindl. cv. Soldam, which possesses hematopoiesis, osteoporosis prevention, and antimutagenic effects, at different growth stages was evaluated. Cytotoxic effect of acetone extracts of immature fruits against various tumor cell lines was higher than that of mature fruits, particularly in hormone-independent human breast cancer, MDA-MB-231 cell line. Immature fruit extract increased expression level of pro-apoptotic protein Bax and reduced that of anti-apoptotic protein Bcl-2, and stimulated caspase-3 activity in MDA-MB-231 cells. Results suggest immature fruit of P. salicina Lindl. cv. soldam to be natural source for development of functional food and medical agents to prevent human breast cancer.