• Title/Summary/Keyword: 위치패턴 분석

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Selection of (Ac/Ds) insertion mutant lines by abiotic stress and analysis of gene expression pattern of rice (Oryza sativar L.) (비생물학적 스트레스 관련 벼 Ac/Ds 삽입 변이체의 선발 및 유전자 발현 분석)

  • Jung, Yu-Jin;Park, Seul-Ah;Ahn, Byung-Ohg;Yun, Doh-Won;Ji, Hyeon-So;Lee, Gang-Sup;Park, Young-Whan;Suh, Seok-Cheol;Baek, Hyung-Jin;Lee, Myung-Chul
    • Journal of Plant Biotechnology
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    • v.35 no.4
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    • pp.307-316
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    • 2008
  • Transposon-mediated insertional mutagenesis is one of powerful strategy for assessing functions of genes in higher plants. In this report, we have selected highly susceptible and tolerance plant by screening about high salt (3% NaCl) and cold stresses ($4^{\circ}C$) from F2 seeds of 30,000 Ac/Ds insertional mutagenesis lines in rice (Oryza sativa L. cv. Dongjin). In order to identify the gene tagging, insertion of Ds element was analyzed by Southern blot and these results revealed that 19 lines were matched genotype of selected lines with phenotype from the first selected 212 lines, and 13 lines have one copy of Ds elements. The Franking Sequence Tags (FSTs) of selected mutant lines showed high similarities with the following known function genes: signal transduction and regulation of gene expression (transpoter, protease family protein and apical meristem family protein), osmotic stress response (heat shock protein, O-methyltransferase, glyceraldehyde-3-phosphate dehydrogenase and drought stress induce protein), vesicle trafficking (SYP 5 family protein) and senescence associated protein. The expression pattern of 19 genes were analyzed using RT-PCR under the abiotic stresses of 9 class; 250mM NaCl, osmotic, drought, 3% $H_2O_2$, $100{\mu}M$ ABA, $100{\mu}M$ IAA, 0.1 ppm 2,4-D, $4^{\circ}C$ cold and $38^{\circ}C$ high temperature. Isolated knock-out genes showed the positive response about 250 mM NaCl, drought, $H_2O_2$, PEG, IAA, 2,4-D, ABA treatment and low ($4^{\circ}C$) and high temperature ($38^{\circ}C$). The results from this study indicate that function of selected knock-out genes could be useful in improving of tolerance to abiotic stresses as an important transcriptional activators in rice.

Influences of the Composition on Spectroscopic Characteristics of AlxGa1-xN Thin Films (AlxGa1-xN 박막의 조성이 분광학적 특성에 미치는 영향)

  • Kim, Dae Jung;Kim, Bong Jin;Kim, Duk Hyeon;Lee, Jong Won
    • New Physics: Sae Mulli
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    • v.68 no.12
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    • pp.1281-1287
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    • 2018
  • In this study, $Al_xGa_{1-x}N$ films were grown on (0001) sapphire substrates by using metal-organic chemical vapor deposition (MOCVD). The crystallinity of the grown films was examined with X-ray diffraction (XRD) patterns. The surfaces and the chemical properties of the $Al_xGa_{1-x}N$ films were investigated using atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS), respectively. The optical properties of the $Al_xGa_{1-x}N$ film were studied in a wide photon energy range between 2.0 ~ 8.7 eV by using spectroscopic ellipsometry (SE) at room temperature. The data obtained by using SE were analyzed to find the critical points of the pseudodielectric function spectra, $<{\varepsilon}(E)>=<{\varepsilon}_1(E)>+i<{\varepsilon}_2(E)>$. In addition, the second derivative spectra, $d^2<{\varepsilon}(E)>/dE^2$, of the pseudodielectric function for the $Al_xGa_{1-x}N$ films were numerically calculated to determine the critical points (CPs), such as the $E_0$, $E_1$, and $E_2$ structure. For the four samples (x = 0.18, 0.21, 0.25, 0.29) between a composition of x = 0.18 and x = 0.29, changes in the critical points (blue-shifts) with increasing Al composition at 300 K for the $Al_xGa_{1-x}N$ film were observed via ellipsometric measurements for the first time.

Development and Validation of a Learning Progression for Astronomical Systems Using Ordered Multiple-Choice Items (순위 선다형 문항을 이용한 천문 시스템 학습 발달과정 개발 및 타당화 연구)

  • Maeng, Seungho;Lee, Kiyoung;Park, Young-Shin;Lee, Jeong-A;Oh, Hyunseok
    • Journal of The Korean Association For Science Education
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    • v.34 no.8
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    • pp.703-718
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    • 2014
  • This study sought to investigate learning progressions for astronomical systems which synthesized the motion and structure of Earth, Earth-Moon system, solar system, and the universe. For this purpose we developed ordered multiple-choice items, applied them to elementary and middle school students, and provided validity evidence based on the consequence of assessment for interpretation of learning progressions. The study was conducted according to construct modeling approach. The results showed that the OMCs were appropriate for investigating learning progressions on astronomical systems, i.e., based on item fit analysis, students' responses to items were consistent with the measurement of Rasch model. Wright map analysis also represented that the assessment items were very effective in examining students' hypothetical pathways of development of understanding astronomical systems. At the lower anchor of the learning progression, while students perceived the change of location and direction of celestial bodies with only two-dimensional earth-based view, they failed to connect the locations of celestial bodies with Earth-Moon system model, and they could recognized simple patterns of planets in the solar system and milky way. At the intermediate levels, students interpreted celestial motion using the model of Earth rotation and revolution, Earth-Moon system, and solar system with space-based view, and they could also relate the elements of astronomical structures with the models. At the upper anchor, students showed the perspective change between space-based view and earth-based view, and applied it to celestial motion of astronomical systems, and they understood the correlation among sub-elements of astronomical systems and applied it to the system model.

A Study of Fishing Ground Distribution in Korean Tuna Long-Line , Using the Catch Data Base System (어획량 데이터베이스 시스템을 이용한 한국 다랭이 연승 어장의 분포에 관한 연구)

  • 이주희
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.32 no.4
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    • pp.340-355
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    • 1996
  • In order to suggest the useful information of fishing ground, a data base system on 32bit personal computer was constructed and handled by using the catch data of Korean tuna long-line, catch by species, fishing time and place, fish price and etc. mainly from 1975 to 1992. The results obtained are as follows ; In the fishing ground displaying catch rate, the catch rate has reduced as time passed, and this penomenon was more evident in Indian. And yellowfin have high catch tate in the Western Pacific of low latitute region, bigeye tuna have in the Eastern. The region of high catch rate of bigeye tuna was moved from the Indian and the Atlantic to the Pacific. The patterns of catch numbers of yellowfin and bigeye tuna appeared nearly same that, having nothing to do with catch numbers in all oceans. The region of least catch was the Northwestern Pacific, the regions of most catch were the Western Indian and the Pacific of low latitute. As to simulation of fishing ground estimation, there were economical grounds in the Western Pacific of low latitute region, the Eastern Pacific of this, the Western Indian, the Eastern Indian, and the Atlantic, in order.

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지질시대 및 지구조별 국내대리석 석재자원의 분류와 물성

  • 윤현수;홍세선;박덕원;이병대;김주용
    • Proceedings of the Mineralogical Society of Korea Conference
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    • 2002.10a
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    • pp.153-169
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    • 2002
  • 국내 대리석류 석재자원은 지질시대 및 지구조별로 선캠브리아기의 경기육괴와 영남육괴, 시대미상의 옥천대 남서부, 캠브리아-오오도비스기의 옥천대 북동부 그리고 일부 기하시대의 옥천대 북동부 등으로 분류될 수 있다. 전자는 변성퇴적암류내에 협재된 결정질 석회암, 화강암질 편마암내 포획된 결정질 석회암 등s으로 경기육괴의 포천, 김포, 온수리, 목계와 신림도폭 그리고 영남육괴의 춘양, 현동, 울진, 중평동 장기리와 장계도폭 등이 해당된다. 시대미상암류는 옥천대 남서부로서 목계 충주 괴산, 강경, 남원과 송정도폭, 그리고 캠브리아기는 풍촌석회암으로 임계, 호명, 서벽리도폭 등이 해당된다. 오오도비스기는 정선석회암이 분포하는 석병산, 평창, 정선과 임계도폭, 화천리층이 분포하는 문경도폭, 그리고 황강리도폭 등이 해당한다. 기타시대는 시대미상의 각력질 석회암과 상부석탄기의 홍점층군내 협재하는 결정질 석회암으로 이들은 각각 정선도폭과 석병산도폭에 위치한다. 선캠브리아기, 시대미상, 캠브리아기 및 오오도비스기 대리석류의 물성 중에서 흡수율과 공극율은 선캠브리아기, 오오도비스기 시대미상과 캠브리아기의 순으로 점차 감소한다 이들의 공극율은 비중에 대하여 대체로 불규칙하며, 흡수율은 공극율에 대하여 거의가 뚜렷한 정의 상관관계를 이룬다. 압축강도는 공극율에 대하여 다소 불규칙한, 그리고 인장강도에 대하여 정의 상관관계를 이룬다. 그리고 마모경도는 압축강도와 인장강도에 대하여 대체로 뚜렷한 정의 경향을 각각 보인다. 이들 대리석류는 선캠브리아기 중경암-경암, 시대미상 중경암-경암, 캠브리아기 거의가 중경암-경암, 그리고 오오도비스기는 경암에 각각 해당한다.역할을 충실히 담당하고 있는 것으로 분석되었다. 그러나 과학기술의 급격한 발달, 소비패턴의 변화, 생활환경과 삶의 질을 중시하는 새로운 가치관의 확산 등으로 광업의 역할도 새로운 변화의 전기를 맞이하고 있음을 볼 수 있다. 국내광업이 21C 급변하는 산업환경에 적응하여 생존하기 위해서는 각종 첨단산업에서 요구하는 소량 다품종의 원료광물을 적기에 공급 할 수 있는 전문화된 기술력을 하루속히 확보해야 하며, 이를 위해 고품위의 원료광물 확보를 위한 탐사 및 개발을 적극 추진하고 가공기술의 선진화를 위해 선진국과의 기술제휴 등 자원산업 글로벌화 정책이 절실히 요구되고 있음을 알 수 있다. 또한 삶의 질을 향상시키려는 현대인의 가치관에 부합하기 위해서는 각종 소비제품의 원료를 제공하는 광업의 본래 목적 이외에도 자연환경 훼손을 최소화하며 개발 할 수밖에 없는 구조적인 어려움에 직면할 수밖에 없다. 이처럼 국내광업이 안고 있는 여러 가지 난제들을 극복하기 위해서는 업계와 정부가 합심하여 국내광업 육성의 중요성을 재인식하고 새로운 마음가짐으로 관련 정책을 수립 일관성 있게 추진해 나가야 할 것으로 보인다.의 연구 결과를 요약하면 다음과 같다. 첫째, 브랜드 이미지와 서비스 품질과의 관계에서 브랜드이미지는 서비스 품질의 선행변수가 될 수 있음을 증명하였으며 4개 요인의 이미지 중 사풍이미지를 제외한 영업 이미지, 제품 이미지, 마케팅 이미지가 서비스 품질에 영향을 미치고 있음을 알 수 있다. 둘째, 지각된 서비스 품질과 가격 수용성과의 관계에서, 서비스 품질은 최소 가격에 신뢰서비스 요인에서 정의 영향을 미치고 있으나 부가서비스, 환경서비스에서는 역의 영향을

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Characteristics of Buckling Load and Bifurcation in Accordance with Rise-span Ratio of Space Truss Considering Initial Imperfection (초기 불완전성을 고려한 공간 트러스의 분기좌굴과 라이즈-스팬 비에 따른 임계하중 특성)

  • Lee, Seung-Jae;Shon, Su-Deok
    • Journal of Korean Society of Steel Construction
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    • v.24 no.3
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    • pp.337-348
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    • 2012
  • This study investigated the characteristics of bifurcation and the instability due to the initial imperfection of the space truss, which is sensitive to the initial conditions, and the calculated buckling load by the analysis of Eigen-values and the determinant of tangential stiffness. A two-free nodes model, a star dome, and a three-ring dome model were selected as case studies in order to examine the unstable phenomenon due to the sensitivity to Eigen mode, and the influence of the rise-span ratio and the load parameter on the buckling load were analyzed. The sensitivity to the imperfection of the two-free nodes model changed the critical path after reaching the limit point through the bifurcation mode, and the buckling load level was reduced by the increase in the amount of imperfection. The two sensitive buckling patterns for the model can be explained by investigating the displaced position of the free node, and the asymmetric Eigen mode was a major influence on the unstable behavior due to the initial imperfection. The sensitive mode was similar to the in-extensional mechanism basis of the simplified model. Since the rise-span ratio was higher, the effect of local buckling is more prominent than the global buckling in the star dome, and bifurcation on the equilibrium path occurring as the value of the load parameter was higher. Additionally, the buckling load levels of the star dome and the three-ring model were about 50-70% and 80-90% of the limit point, respectively.

3D Numerical Study on the Reinforcing Effect of Inclined System Bolting in NATM Tunnel (NATM 터널에서 경사 록볼트의 보강효과에 대한 3차원 해석)

  • Heo, June;Kim, Byoung-Il;Lee, Jea-Dug;Kim, Young-Geun
    • Journal of the Korean Geotechnical Society
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    • v.33 no.3
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    • pp.29-36
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    • 2017
  • It has been known that rockbolt is one of important supports improving the support capacity with shotcrete in NATM tunnel. Also, it is necessary for the inclined system bolting to enhance the efficiency of installation in case of a narrow space such as cross passage and enlargement tunnel. However, there is no profound technical study for the effect of inclined rockbolt of systematic installation on the support mechanism and ground behaviour in NATM tunnel. In this study, the effects of the length and installation angle of rockbolt on the characteristics of support and ground reinforcement were analyzed by using 3D finite element numerical study. Through the numerical results for the parametric modelling of inclined rockbolt, the characteristics of mechanical behaviors between the axial force of rockbolt and the effect of ground reinforcement in regard to the various factors of the length and installation angle of rockbolt were verified and reviewed thoroughly. Also, it was shown that the installation angle of rockbolt for enhancing the arching effect in NATM tunnel was $45^{\circ}$, and the difference of the reinforcing effect for support between the installation angles of $75^{\circ}$ and $90^{\circ}$ was insignificant. The additional numerical studies for various condition would be carried out for practical design guideline of inclined rockbolt.

An Anti-Collision Algorithm with 4-Slot in RFID Systems (RFID 시스템에서 4 슬롯을 이용한 충돌방지 알고리즘)

  • Kim, Yong-Hwan;Kim, Sung-Soo;Ryoo, Myung-Chun;Park, Joon-Ho;Chung, Kyung-Ho
    • Journal of the Korea Society of Computer and Information
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    • v.19 no.12
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    • pp.111-121
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    • 2014
  • In this paper, we propose tree-based hybrid query tree architecture utilizing time slot. 4-Bit Pattern Slot Allocation(4-SL) has a 8-ary tree structure and when tag ID responses according to query of the reader, it applies a digital coding method, the Manchester code, in order to extract the location and the number of collided bits. Also, this algorithm can recognize multiple Tags by single query using 4 fixed time slots. The architecture allows the reader to identify 8 tags at the same time by responding 4 time slots utilizing the first bit($[prefix+1]^{th}$, F ${\in}$ {'0' or '1'}) and bit pattern from second ~ third bits($[prefix+2]^{th}{\sim}[prefix+3]^{th}$, $B_2{\in}$ {"00" or "11"}, $B_1{\in}$ {"01" or "10"}) in tag ID. we analyze worst case of the number of query nodes(prefix) in algorithm to extract delay time for recognizing multiple tags. The identification delay time of the proposed algorithm was based on the number of query-responses and query bits, and was calculated by each algorithm.

Thermal-hydro-mechanical Modelling for an Äspö prototype repository: analysis of thermal behavior (Äspö 원형 처분장에 대한 열-수리-역학적 모델링 연구: 열적 거동 해석)

  • Lee, Jae Owan;Birch, Kenneth;Choi, Heui-Joo
    • Tunnel and Underground Space
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    • v.23 no.5
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    • pp.372-382
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    • 2013
  • Thermal-hydro-mechanical (THM) modeling is a critical R&D issue in the performance and safety assessment of a high-level waste repository. With an $\ddot{A}$sp$\ddot{o}$ prototype repository, its thermal behavior was analyzed and then compared with in-situ experimental data for its validation. A model simulation was used to calculate the temperature distributions in the deposition holes, deposition tunnel, and surrounding host rock. A comparison of the simulation results with the experimental data was made for deposition hole DH-6, which showed that there was a temperature difference of $2{\sim}5^{\circ}C$ depending on the location of the measuring points, but there was a similar trend in the evolution curves of temperature as a function of time. It was expected that the coupled modeling of the thermal behavior with the hydro-mechanical behavior in the buffer and backfill of the $\ddot{A}$sp$\ddot{o}$ prototype repository would give a better agreement between the experimental and model calculation results.

Long-term Prediction of Bus Travel Time Using Bus Information System Data (BIS 자료를 이용한 중장기 버스 통행시간 예측)

  • LEE, Jooyoung;Gu, Eunmo;KIM, Hyungjoo;JANG, Kitae
    • Journal of Korean Society of Transportation
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    • v.35 no.4
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    • pp.348-359
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    • 2017
  • Recently, various public transportation activation policies are being implemented in order to mitigate traffic congestion in metropolitan areas. Especially in the metropolitan area, the bus information system has been introduced to provide information on the current location of the bus and the estimated arrival time. However, it is difficult to predict the travel time due to repetitive traffic congestion in buses passing through complex urban areas due to repetitive traffic congestion and bus bunching. The previous bus travel time study has difficulties in providing information on route travel time of bus users and information on long-term travel time due to short-term travel time prediction based on the data-driven method. In this study, the path based long-term bus travel time prediction methodology is studied. For this purpose, the training data is composed of 2015 bus travel information and the 2016 data are composed of verification data. We analyze bus travel information and factors affecting bus travel time were classified into departure time, day of week, and weather factors. These factors were used into clusters with similar patterns using self organizing map. Based on the derived clusters, the reference table for bus travel time by day and departure time for sunny and rainy days were constructed. The accuracy of bus travel time derived from this study was verified using the verification data. It is expected that the prediction algorithm of this paper could overcome the limitation of the existing intuitive and empirical approach, and it is possible to improve bus user satisfaction and to establish flexible public transportation policy by improving prediction accuracy.