• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.88-88
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• 2002

Erythropoietin (EPO)는 조혈작용 (erythropoiesis)을 나타내는 호르몬으로서 사람의 빈혈치료제로 사용되며, 포유통물 중 사람, 생쥐 등의 유즙 내에 혈청 EPO 와 동일한 크기로 다량으로 존재한다고 보고된 바 있다. 생쥐의 WAP promoter를 이용하여 사람의 조혈촉진제인 EPO를 유즙으로 생산하는 형질전환돼지 (새롬이)의 유즙을 분석하기 위해 SDS-PAGE와 Western blotting 을 수행하였다. 먼저, 형질전환돼지의 유즙으로부터 원심분리에 의해 지방층을 제거한 후, 16.5% polyacrylamide gel 에서 PAGE를 수행하였다. (중략)

• Food Engineering Progress
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• v.14 no.4
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• pp.346-353
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• 2010

Due to the globalization of Korean foods, there are great interests in traditional Korean foods. Thus, the enhancement and development of makgeolli processing have been constantly accomplished. In case of makgeolli, the storage stability is very important because the fermentation of makgeolli during distribution is still progressed. Therefore, the objective of this study was to investigate storage stability of makgeolli by separation storage methods. During the 30-day storage at $10^{\circ}C$, pH value, titratable acidity, color value, sugar content, reducing sugar content, and alcohol content were measured. Microbial cell counts were also evaluated. Reducing sugar content was decreased after 10 days for all the samples. In the case of titratable acidity and color, these values were constantly increased with storage time. Especially, the yellowness value of the precipitate of makgeolli was increased by two times than that of the beginning. There was a decreasing tendency for lactic acid bacteria with storage time. In case of yeast, there was a decreasing tendency after 15 days, but the significance was not detected. The quality changes in the samples from centrifugal separation were relatively less than the control. Therefore, the separation storage method could affect the enhancement of makgeolli quality during distribution.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.803-806
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• 2002

This paper estimated the stability of centrifuge as its rotating speed compared with the natural frequency of cylinder and screw by finite element analysis. It shows that the one of exciting causes is the tooth passing frequency of input and output parts by the periodic characteristics of transmission error due to an planetary gear having three stages. Also, it proposed the critical speed of cylinder and screw by the Campbell diagram.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.11
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• pp.108-116
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• 2003

In this paper, based on the concept of solid particles' sediment problem the working formula determining the bowl length of a Decanter-type centrifuge were derived. Assuming that particles are uniformly distributed along the vertical line of the liquid inlet position, it was possible to derive a constant value k used for determining the bowl length. It was shown from the sample calculations that the bowl length should be increased as the particle size to be removed from the liquid is decreased. The length also should be increased for the same particle size as the bowl diameter is decreased. To help the engineers choose a reasonable bowl diameter, the statistical relationship between the bowl diameter and the capacity of the international products is obtained and presented.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.7
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• pp.929-937
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• 2003

In general, the electric motor for driving the centrifuge of Decanter type is selected based on the power(starting power) necessary to start the bowl and the screw shaft. However the operation cost of the machine is dependent on the power needed at a steadily operating condition, including the power against the bearing friction and that for the sludge removal. In this paper, the formulation for the sludge-removal power is presented. Sample calculation for a specific design shows that the sludge-removal power is increased with the friction coefficient. It also reveals that the power is mainly dependent on the length of the screw blade rather than the beach angle. Further it is shown that the power increases in square of the rotational speed of the machine.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.23 no.4
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• pp.36-41
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• 2015

The cyclone separator is a simple device, which causes the centrifugal separation of materials such as droplets or particles in a fluid stream. The cyclone separator utilizes the energy obtained from fluid pressure and linear motion to create rotational fluid motion. This rotational motion leads the materials suspended in the fluid to separate from the fluid quickly due to the centrifugal force. The rotation is produced by the tangential or involuted introduction of fluid into the vessel. These materials may be droplets of fuel in blow-by gas through an engine. Droplets suspended in the feed liquid may separate according to size, shape, or density. And the change of part dimension in a cyclone separator can yield the its performance variation. The current study shows the influence of design parameters on the performance of a cyclone separator for blow-by gas.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.19 no.4
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• pp.576-581
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• 2010

As a super high speed small-sized centrifugal separator which is operated by the center inputting method has solved two difficulty problems, it can be made use of developing the new composition through the making of Nano particle (50~100nm) by high speed revolution and alleviation of unbalanced shapes inside the disk continuously throwing slurry in the central part. Uses the air spindle and the centrifugal machine production will be able to manufacture the Roh class fine particle under boil.

• Journal of the Korean Society for Precision Engineering
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• v.24 no.6
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• pp.78-85
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• 2007

In this study, we performed finite element analysis for the design of a medical centrifuge and two-types of centrifuge were compared with each other. The types of centrifuge are 2-arm straight type and 3-arm type. Structural analysis was done with respect to the change of the rotational speed of the rotor of a centrifuge. When the rotor of centrifuge was rotated, the von Mises stress of 2-arm straight type-rotor was compared with the von Mises stress of 3-arm type. The margin of safety was estimated from the result. We found the critical speed of centrifuge from the campbell diagram by modal analysis.

• Microbiology and Biotechnology Letters
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• v.30 no.2
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• pp.184-188
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• 2002

A bacteriocin producing microorganism, which inhibits the growth of Lactobacillus sake, was screened and isolated from Kimchi. This microorganism was identified and named as Lactobacillus sp. Oh-B3, The maximum amount of bacteriocin was produced when the isolated microorganism was cultured in MRS media(pH 8.0) for 24 hours at 25℃. The bacteriocin from the isolated microorganism was purified through ammonium sulfate precipitation, dialysis and ultrafiltration. The bacteriocin was stable on the wide pH range of 2.0-9.0, and showed antimicrobial activity on some of gram positive bacteria, not on gram negative. The antimicrobial activity of bacteriocin was mostly removed by treatment of proteolytic enzymes. But, the bacteriocin was very stable on the heat treatment, and more than 50％ of activity was remained at autoclaving. The action mode of the bacteriocin showed bacteriocidal pattern, being same as that of general bacteriocins.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2008.11a
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• pp.190-191
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• 2008