• Journal of the Society of Cosmetic Scientists of Korea
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• v.11 no.1
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• pp.21-31
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• 1985

The effect of dispersed phase on the stability of emulsion system with polyoxyethylene dodecyl ethers and their variation of ethylene oxide moles was studied by such methods as interfacial tension measurement, centrifugation and droplet size variation with time. The experiments showed that, in interfacial tension measurement, long chain alkanols into dispersed phaes are more effectively adsorbed onto interface, while long chain alkanes nearly not, and in centrifugation, dispersed phase with alkanols is less separated than that with alkanes. On the other hand, alkanes help more stabilyzing emulsion than alkanols in droplet size variation. And the addition of NaCl or Urea, and variation of E.O. moles have very slight effects on the stability with alkanes than with alkanols. Moreover, the longer carbon chain length is, dispersed phase is more effective on emulsion stability. Supposed from these facts is that more stable emulsion can be made with alkanes which retard molecular diffusion by water solubility decrease rather than alkanols which raise resistance to coalescence by rigid interfacial mixed monolayers formation. In conclusion, the stabilities of these emulsions are proved to be more influenced by molecular diffusion than coalescence.

• Journal of the Korean Wood Science and Technology
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• v.17 no.3
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• pp.61-66
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• 1989

포플라 세포(細胞)의 Sucrose gradient 원심분리(遠心分離)에 나타난 세포(細胞)분획은 6개의 band 및 침전으로 분리(分離) 되었다. Peroxidase의 활성변화(活性變化)는 휴면기(休眼期)에 높아졌으며, 자발적(自發的) 휴면(休眠)이 타파되면서 낮아지기 시작하였다. 휴면기(休眠期)에 급격히 높아진 Peroxidase 활성(活性)은 Peroxidative 활성(活性)을 지닌 세포내(細胞內) Microbody에 존재(存在)하는 것으로 사료된다. Catalase의 활성(活性)은 11월(月), 12 월(月) 1월(月)의 휴면기(休眼期)에 활성(活性)이 낮았으며. IAA의 농도는 휴면초기(休眼初期)에 감소하기 시작하여, 12월(月)에 최저(最低)가 되었다. 자연휴면(自然休眠)이 타파된 1월(月)부터는 IAA 농도가 증가하기 시작하여 4월(月)의 발아기(發芽期)까지 계속되었다. 이와같은 Peroxidase, Catalase를 비롯한 IAA의 변화(變化)는 봄철의 발아(發芽) 및 재생장(再生長)과 가을철 휴면조절(休眠調節)에 영향을 미치는 것으로 사료된다.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.1
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• pp.130-136
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• 1998

A procedure of stress and strength analysis has been proposed for the centrifuge rotor of composite materials of quasi-isotropic laminates. The goal in this study is to maximize the allowable rotating speed, that is, to minimize maximum strength ratio with the given path length by changing the geometric parameter-outer radius and ply angles in quasi-isotropic laminates. Optimum values of the geometric parameter-outer radius and ply angles are obtained by multilevel optimization. All the geometric dimensions and stresses are normalized such that the result can be extended to a general case. Two dimensional analysis at each cross section with an elliptic tube hole subjected to internal hydrostatic pressures by samples as well as the centrifugal body forces has been performed along the height to calculate the stress distribution with the plane stress assumption, and Tsai-Wu failure criterion is used to calculate the strength ratio. The maximum allowable rotating speed can be increased by changing the radii of the outer surface along the height with the maximum strength ratio under the unit value : The optimal number of ply angles maximizing the allowable rotating speed in quasi-isotropic laminates is found to be the half number of tube hole, and the optimal laminate rotation angle is the half of $[{\pi}/m]$. A $[{\pi}/3]$ laminate, for instance, is stronger than a $[{\pi}/4]$ laminate for the centrifuge rotor of 6 tube hole number even though they have the same stiffness.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.7
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• pp.938-947
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• 2003

In this paper, we derived the formula for estimating the power of the electric motors needed to operate the Decanter-type centrifuge. In the derivation of the formula the sludge-removal torque is to be supplied from the formula derived in the first paper. The intricate nature of the transmission mechanism in the planetary gear trains of the sludge-removal power and torque has been clarified in this second paper. In particular we considered two-motor system, where the main motor drives the machine while the differential-speed control motor plays the role of braking in adjusting the differential speed. Sample calculation for the specific design treated in the first paper showed that the selection criterion for the main motor depends on the lower limit of the differential speed; when the lower limit is set low, it should be selected based on the steadily operating power, while it should be selected based on the starting power when the lower limit is set high. The total power required by both the main motor and the differential-speed control motor increases as the differential speed is decreased. It is suggested that the power loss in the differential-speed control motor could be minimized by attaching an electric generator to it.

• Korean Journal of Food Science and Technology
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• v.19 no.5
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• pp.441-445
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• 1987

The subcellular distribution of lipoxygenase in germinating soybean seeds (Glycine max[L.] AmSoy) was investigated by using differential centrifugation and sucrose density gradient fractionation. Most of lipoxygenase -1 and -2/3 activities was present in the supernatant fraction after differential centrifugation of homogenates prepared from three-day-old seedlings; only 1.5% of lipoxygenase activity remained in particulate fractions. The results of a sucrose density gradient fractionation (three-day-old) showed that the lipoxygenase activity coincided with acid phosphatase at the densities of 1.19, 1.23, $1.25g/cm^3$, even though most of lipoxygenase and acid phosphatase activities appeared in supernatant fractions. There was no indication that mitochondria contained any lipoxygenase activity, and it does not appear that glyoxysomes and ER contained any lipoxygenase activity either.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.315-319
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• 1985

Delta-endotoxin crystals of B. thuringiensis var. kurstari and B. thuringiensis var. israelensis were purified by NaBr density gradient centrifigation and the wet weight of the BTK endotoxin was approximately 23.79％ of the cell wet weight and that of BTI was 25％. The shape of BTK crystal was bipyramidal, whose size was 1.7${\mu}{\textrm}{m}$ $\times$ 0.9${\mu}{\textrm}{m}$ and that of BTI was a spheroid, whose size was about 1.6$\times$0.45${\mu}{\textrm}{m}$. The molecular weight of BTK crystal protein was approximately 134,000 daltons and that of BTI was about 128,000 daltons.

• Korean Journal of Environmental Biology
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• v.29 no.4
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• pp.305-311
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• 2011

The distribution of flocculating activity of culture broth was examined and the major constituent with flocculating activity was identified. Most of flocculating activity was found in culture broth without cells. As the activity was maintained by the digestion with pronase, it suggests that the activity is due to the polysaccharide. The bioflocculant obtained from $Lactobacillus$ $jensenii$ YW-33 was precipitated by 60~80% EtOH fractionation (LJ-80). LJ-80 was separated by ion-exchange chromatography using DEAE-Toyopearl 650C and LJ-80-II showed more potent flocculating activity than those of other fractions. The major activity fraction LJ-80-II was further purified on the gel permeation using Sepharose CL-6B to LJ-80-II-1. GPC (Sepharose CL-6B) and HPLC were used to determine whether LJ-80-II-1 has a homogenecity. The molecular weight of purified LJ-80-II-1 was estimated over 800,000 dalton by gel permeation chromatography. Purified LJ-80-II-1 contained 98.4% total sugar, 0.6% protein. Main sugar of purified LJ-80-II-1 was composed of mannose : galactose : glucose with a molar ratio of 1.61 : 0.25 : 1.00.

• Applied Biological Chemistry
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• v.46 no.2
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• pp.69-73
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• 2003

A novel antifungal antibiotic for azole-resistant Candida albicans was purified from the culture broth of Bacillus subtilis LAM 97-44 by butanol extraction, Diaion HP-20 and Dowex-50 adsorption chromatography, silica gel flash chromatography followed by HPLC and designated LAM-44A. LAM-44A was stable for 60 min at $100^{\circ}C$, and pH range from 2 to 10. MIC values were observed at $0.5-3.5\;{\mu}g/ml$ against various Candida albicans strains. The antibiotic showed no cytotoxicity for S180, MKN-45, P388, HeLa and 373 at the concentration of 1 mg/ml. LAM-f4A was colorless powder soluble in water, methanol, ethanol, butanol and negative to ninhydrin reaction. The antibiotic had maximum absorption at 273 nm in methanol, and melting point was $202^{\circ}C$. The molecular weight and formula were determined to be 282 and $C_{14}H_{34}O_5$ by $^1H-NMR,\;^{13}C-NMR$, IR spectrum and elemental analysis.

• Journal of Sericultural and Entomological Science
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• v.30 no.2
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• pp.96-104
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• 1988

Vitellin, the major yolk protein of the silkworm, Bombyx mori was pruified, and its immunological properties and the quantitative changes during embryogenesis were studied. The ovary transplantation into male hosts was also carried out to find its effect on the yolk protein synthesis. The pupal vitellogenin and the egg vitellin of Bombyx mori were purified by DEAE-cellulose column chromatography. These two female specific proteins showed the same mobility in the polyacrylamide gel electrophoresis and the same reaction in the double immunodiffusion test. The immunological identity was also observed between the vitellins of Bombyx mori and Bombyx mandarina. The rudimentary ovaries transplanted into the male hosts of silkworms produced eggs without vitellin, indicating that the yolk precursors synthesized in other female organ beyond the ovary were necessary to produce vitellins. The major yolk protein, vitellin was disintegrated and utilized mostly during late stage of embryogenesis. It was different characteristics from the egg specific protein, which was utilized continuously from the early embryonic stage.

• KSBB Journal
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• v.14 no.6
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• pp.677-681
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• 1999

Purificationi of egg yolk immunoglobulin(IgY) was performed to understand the property of egg immunoglobulin. IgY differs from mammalian IgY in the molecular size(larger), isoelectric point(more acidic), and binding ability with mammalian complement and protein A(nonbinding ability). IgY is also known as ${\gamma}$-livetin and exists in egg yolk together with other two water-solubel proteins, ${\alpha}$-livetin(chicken serum albumin) and ${\beta}$-livetin(${\alpha}_2$-glycoprotein) and various lipoproteins(Low density lipoprotein, LDL and High density lipoprotein, HDL) which are the major components of egg yolk. The first step of isolation of IgY is to separate the water-solube proteins from lipoproteins. We report a simple method for separation of water soluble proteins using k-carrageenan and sedimentation. k-carrageenan was found to be effective for removal of yolk lipoprotein as a precipitate. IgY remained supernatant, and was isolated by chromatography on columns of DEAE-Sephacel and G 75 gel filtration chromatography.