• Proceedings of the Membrane Society of Korea Conference
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• 2004.05b
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• pp.98-101
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• 2004

최근에 이루어지고 있는 생명공학의 성장에 따라 단백질을 대량으로 정제할 수 있는 공정 개발에 대한 필요성이 부각되고 있다. 이러한 목적으로 기존에 이루어지고 있는 침전법, 결정화법 그리고 원심분리법과 같은 방법은 선택도가 매우 떨어지는 단점이 있으며, 반면 크로마토그래피 및 전기영동과 같은 방법은 탁월한 선택도에 비해 그 규모가 매우 작은 경우 에 알맞은 방법으로서 대량생산 체계에서는 적합하지 못하다는 것이 그 단점이다.(중략)

• Korean Journal of Animal Reproduction
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• v.17 no.4
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• pp.339-346
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• 1994

An understanding of the structure and function of mammalian spermatozoa requires the iso-lation of these components. In this study, frozen-thawed bovine spermatozoa were treated by physical treatments (vortexing, 26 gauge needle, strained 26 gauge needles and freezing-thawing) or chemical treatments (trypsin, dithiothreitol, sodium dodecylsulfate and $\beta$-mercaptoethanoJ) to yield free heads and tails. The most effective treatment was repeated pumping of sperm suspension through a strained 26 gauge needle conneted to a syringe. Spermatozoa by this treatment were mainly broken at the junction of the head and the tail, resulting in 90-100% yields. Also, sperm head surface did not modify during strained 26 gauge needle treatment when either spermatozoa or sperm heads were incubated in 250${\mu}\textrm{g}$/ml of FITC-UEA 1 for 1 h at room temperature to detect the modification of sperm surface components. Other physical treatments were less efficient for the breakdown of spermatozoa. The effects of chemical treatments on bovine spermatozoa are not noticeable. Dissected sperm heads and tails should be fractional leading to nearly pure components by sucrose gradient centrifugation at 1,000 rpm for 15 min. The result suggest that the established method may be useful for the biochemical study of spermatozoal components, and the understanding of oocyte activation mechanism either by spermatozoal components during fertilization or microinjection of isolated components.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.207-210
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• 2013

In this study, matured silkworm hemolymph was studied for inhibitory effect on melanin formation, tyrosinase activities. A matured silkworm hemolymph mass purification method was developed and some of suppressing melanin formation effects were investigated, too. As a result, the advanced purification method was higher in yield rate than existing one by 53%. A free radical scavenging activity of hemolymph was higher than sericin on the market($IC_{50}$, $202.9{\pm}33.1$) and lower than vitamin C($0.57{\pm}0.02$) by $6.4{\pm}0.3{\mu}g/m{\ell}$. An inhibitory effect on melanin synthesis was higher in hemolymph compared to Morus bark and arbutin by 9.15% and 11.56%, respectively. An inhibitory effect on tyrosinase activity was higher in hemolymph than Morus bark and lower than arbutin. Effect of hemolymph $45{\mu}g/m{\ell}$ on suppressing of tyrosinase was almost 90%. Thus, these results suggest that hemolymph of matured silkworm may have beneficial properties as a material for cosmetics.

• Nuclear industry
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• v.8 no.7 s.65
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• pp.90-92
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• 1988

일본에서는 금년도에 들어와 우라늄농축기술 개발이 활기를 띠고 있다. 동력로$\cdot$핵연료개발사업단이 '84년부터 건설한 원심분리법에 의한 우라늄농축원형플랜트가 4월 25일 본격적인 가동에 들어갔으며, 정부의 지원을 받아 욱화성이 연구개발을 추진해온 화학교환법에 의한 우라늄농축이 세계최초로 5월 16일 원자력발전소의 핵연료로 사용할 수 있는 $3\%$ 농축에 성공하였다. 다음은 일본의 우라늄농축기술 개발현황이다.

• The Science & Technology
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• v.28 no.10 s.317
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• pp.18-19
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• 1995

매분 10만을 넘게 회전하는 초고속 모터가 상품화 되고 있어 모터의 마하시대가 열리고 있다. 초고속 모터에 의해 탄생된 초고속 원심분리기는 유전자 산업과 의료분야에서 폭넓게 활용되고 있는데 분당30만회전하는 초고속 모터의 등장도 멀지 않아 실현되어 산업계 전반에 획기적인 변혁이 기대되고 있다.

• Proceedings of the Korean Ceranic Society Conference
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• 2002.10a
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• pp.41.1-41
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• 2002

• Korean Journal of Veterinary Research
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• v.20 no.1
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• pp.1-10
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• 1980

매추리 유래 가금 adenovirus인 QBV로 부터 CsCl을 사용한 균질밀도분배원심분리법으로 AAAV의 분리를 시도하였으며, 분리된 바이러스의 순수성을 전자현미경 및 혈청학적인 방법을 통하여 증명하였으며 순수 분리된 AAAV를 항원으로 사용한 혈청반응을 개발하였다. 즉, CsCl의 밀도 1.38 및 $1.42gm/cm^3$의 분획들은 순수한 AAAV이었으며 $1.42gm/cm^3$의 분획은 토끼에서 양성혈청제조용 면역원으로 1.38 및 $1.42gm/cm^3$의 분획은 혈청반응용 표준항원으로 사용하였다. AAAV 표준항원 및 가토 양성혈청계는 MDV, IBDV, ITV 및 "helper"인 가금 adenovirus와의 교차반응이 없었으며 혈청학적인 특이성이 인정되었다. 또한 상기 혈청학적 반응을 이용하여 AAAV의 동정은 물론 AAAV에 대한 닭의 항체검출을 가능하게 하였다.

• Journal of Advanced Marine Engineering and Technology
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• v.41 no.1
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• pp.44-49
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• 2017

In this research, a purifying system using a centrifugal filter equipped with a water separation component has been studied. Oil utilized for lubrication and cooling in marine engines becomes unable to achieve its original purpose over time as the accumulation of solid particles and water increases. Therefore, methods applying the Bernoulli equation to eliminate water in oil were investigated and proposed. As a result, a practical purifying system was designed and tested by an officially certified organization. The new purifying system is anticipated to contribute to the extension of the lifetime of oil in addition to damage reduction methods of mechanical parts.

• Microbiology and Biotechnology Letters
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• v.14 no.6
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• pp.433-439
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• 1986

Abundant amount of Chiamydia trachomatis could be amplified in mammalian McCoy cells and purified using descontinuous Uroarafin gradient centrifugation. As a chemical means io increase the Chlamydia trachomatis inclusions in McCoy cells IUdR treatment was found to be more effective than the cycloheximide treatment and was recommendanble for the proliferation of Chlamydia trachomatis. Centrifugation promoted Chlamydia trachomatis adhesion to McCoy cell surface, and maximal percentage of infected cells was obtained at about 3000g. The purified Chlamydia trachomatis could be kept in SPG solution for 48 hours at +4$^{\circ}C$ but for longer storage freezing to -7$0^{\circ}C$ was necessary.

• Journal of the Korean GEO-environmental Society
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• v.15 no.3
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• pp.49-55
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• 2014

Characteristics of sedimentation of dredged soil is depended on the field condition and characteristics of dredged soil because dredged fill ground was formed by various field condition, sedimentation and self-weight consolidation process of dredged soil. Dredged fill ground is formed as separated sedimentary layer by characteristics of dredged soil. Therefore, it requires some special test method to consider a various field condition, characteristics of sedimentation and self-weight consolidation of dredged soil. In this study, in order to identify the characteristics of sedimentation of dredged soil with disposing velocity geo-centrifuge test and laser particle size analyzer were performed. As a results, river and mixed dredged soil show the separation sedimentation by soil particle size. And sedimentation of clayey dredged soil is parallel to the bottom surface of dredged fill space.