• Proceedings of the Korean Environmental Sciences Society Conference
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• 2007.05a
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• pp.453-456
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• 2007

가금류의 도축 부산물로 대량 배출되고 있는 우모는 주로 생물학적으로 난분해성 단백질인 케라틴으로 구성되어 있다. 따라서 물리화학적 처리에 의하여 우모를 처리하고 있으나 이 방법은 환경오염을 유발한다. 따라서 본 연구에서는 환경친화적 우모 분해 공정을 개발하고, 우모 분해산물의 식물 성장을 위한 비료로서의 가치를 평가하기 위하여 퇴비화 볏짚에서 케라틴 분해효소 생성능이 우수한 균주인 RS7을 분리하였다. RS7의 생화학적 특성과 16S rDNA의 염기 서열을 분석하여 동정한 결과, Bacillus pumilus RS7로 동정되었다. B. pumilus RS7은 $30^{\circ}C$에서 배양 5일 만에 우모를 완전히 분해할 수 있었다. 본 균주에 의한 우모 분해산물은 Helianthus sannuus L.의 생장율, 잎 수 증가량, 개화율, 건조 생체량에 있어 대조군과 화학적 우모 분해산물보다 우수하였다. Bacillus pumilus RS7에 의한 우모 분해산물은 식물 성장 촉진을 위한 미생물기원 비료로써의 역할을 수행할 수 있었으며, 이에 따라 양계산업에서 배출되는 우모 폐기물이 환경에 주는 악영향을 감소시킬 수 있을 것으로 예측된다.

• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.603-612
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• 2004

This study was carried out to investigate the replacing fish meal or soybean meal effect of with feather meal or Bacillus sp. inoculated feather meal in broiler diets on the performances and nutrient utilization. One hundred and eighty broilers were randomly allotted to six dietary treatments(1) control, basal diet; (2) BFMl00, Fish meal replacing at 100% level with bacillus sp. inoculated feather meal; (3) BSM20, Soybean meal replacing at 20% level with bacillus sp. inoculated feather meal; (4) BFMl00+BSM20, Fish meal and soybean meal replacing at 100% level and 20% level with bacillus sp. inoculated feather meal, respectively; (5) GFMl00, Fish meal replacing at 100% level with general feather meal; and (6) GSM20, Soybean meal replacing at 20% level with general feather meal) in a 5 week feeding trial. In overall period, body weight gain of control was the highest(1,623g) and those of BFM 100, BFM 100+ BSM 20 and GFM 100 were 1,572g, 1,564g, and 1,078g, respectively. And that of GFM 100(1,078.3g) was the lowest(p<0.05) among treatments. Digestibility of dry matter for BFM 100+BSM 20(81.46%) was higher(p<0.05) than that for other treatments, and that of crude protein was the lowest (p<0.05). Digestibilities of organic matter were significantly(p<0.05) improved when Bacillus sp. inoculated feather meal was replaced at 100% level with fish meal in the basal diet. Methane and hydrogen sulfide gases from the feces were significantly(p<0.05) decreased in chicks fed the control, BFM 100 and GFM 100 diets, when observed after 3 weeks of feeding trials. Feed costs of the control and BFM 100 were 417 and 384 won, respectively but that of BSM 20 was 558 won. Therefore, replacement of fish meal with Bacillus sp. inoculated feather meal in the diets for chicks could be useful for economic production.

• Korean Journal of Poultry Science
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• v.14 no.2
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• pp.103-108
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• 1987

This study was conducted to bioassay of amino acid availability of feather meal processed by a different methods, commercial feather meal and raw feather meal, The feather meals were processed by labolatory pressure cooker(autoclave) at 2kg/$\textrm{cm}^2$ for 30 minutes ; 3kg/$\textrm{cm}^2$ for 90 minutes : 4kg/$\textrm{cm}^2$ for 120 minutes. Chick employed in the present experiment were Abor Acre strain, male or meat type (body weight, 100-140g), fed with semipurified diet and protein free diet was given during the determination of the metabolic and endogeneous amino acid. The contents of amino acid of samples were investigated by ion-exchange chromatography. The results were as follows; 1. The amino acid availability of raw, 2kg/$\textrm{cm}^2$ for 30 minutes, commercial, 4kg/$\textrm{cm}^2$ for 120 minutes and 3kg/$\textrm{cm}^2$ for 90 minutes of feather meal were -3.09, 63.28, 67.47, 71.22 and 73.75% respectively. 2. The essential amino acid availability of raw, 2kg/$\textrm{cm}^2$ for 30 minutes, commercial, 4kg/$\textrm{cm}^2$ for 120 minutes and 3kg/$\textrm{cm}^2$ for 90 minutes of feather meal were 2.55, 66.78, 66.89, 72,56 and 73.62%, respectively. 3. In individual amino acid of the different processing loather meal and commercial feather meal, biovailabilities were increased methionine, phenylalanine, leucine, arginine, threonine, isoleucine, however, histidine, lysine and aspartic acid were remarkely decreased. In conclusion, the bioavailability or amino acid for the feather meal processed at 3kg/$\textrm{cm}^2$ for 90 minutes was superior to those of other treatment or raw feather meal.

• KOREAN POULTRY JOURNAL
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• s.13
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• pp.62-65
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• 1970

• KOREAN POULTRY JOURNAL
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• v.3 no.10 s.24
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• pp.35-37
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• 1971

• Monthly Korean Chicken
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• v.18 no.12
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• pp.110-115
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• 2012

• Monthly Korean Chicken
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• v.4 no.6 s.36
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• pp.1-1
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• 1998

• Korean Journal of Poultry Science
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• v.36 no.4
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• pp.343-350
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• 2009

This study was carried out to investigate the possibility to utilize feather meal by bacterial strains. A bacterial strain SE-103 producing keratinolytic enzyme was isolated from the soil of the poultry slaughterhouses. It was identified as Bacillus sp. by judging from its morphological and physiological characteristics. Subsequently the optimal culture conditions for the production of keratinolytic protease by Bacillus sp. SE-103 were investigated. The composition of optimal medium was 3.0% glucose, 0.4% urea, 0.2% $NaNO_3$, and 0.15% KCl. In addition, optimal initial pH and temperature were 6.0 and $35^{\circ}C$, respectively.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2006.11a
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• pp.432-435
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• 2006

전 세계적으로 방대하게 배출되는 도축 부산물인 우모는 높은 영양적 가치를 가짐에도 불구하고 현재 사용하는 물리화학적 처리법의 여러 단점으로 효율적으로 이용되지 못하고 있는 실정이다. 그 결과 2차 오염을 발생시키지 않는 경제적 방법인 생물학적 처리에 대한 연구의 필요성이 커지고 있다. 본 연구에서는 그러한 방법 중 대표적인 방법으로 미생물이 생산하는 keratinase를 이용한 생분해에 대해 연구를 하기 위해 경남 일대 퇴비화 볏짚에서 keratinolytic protease 생성능이 우수한 균주인 RS7을 분리하였고 생화학적 동정법과 16S rDNA를 이용한 동정결과 B. pumilus로 동정되었다. 본 실험에서 분리된 B. pumilus는 기존에 활발히 연구되어 있지 않는 균주로써 native feather 분해도가 기존에 알려진 Bacillus 속들이 3일 정도에서 분해 완료되는 것과는 달리 36시간 ${\sim}$48시간 내에 깃대까지 완전히 분해하였다. 또한 분리 균주의 경제적인 분해능을 토대로 분해산물이 식물 생장에 주는 영향과 아미노산 함유량을 검토한 결과 기존의 화학적 분해에 의한 분해산물보다 아미노산 함유량이 4배가량 많았으며 식물 생장에 있어서도 생장율과 개화시점으로 미루어볼 때 비료로써의 역할을 수행할 수 있었다.

• Korean Journal of Poultry Science
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• v.24 no.4
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• pp.185-191
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• 1997

In order to obtain the basic information needed to utilize poultry by-products as feed resources, the yielding ratio, chemical composition and nutrient bioavailability of 5 offal components such as autoclaved head, feet, viscera, blood and feathers were investigated. Yielding ratios of head, feet, viscera, blood and feathers were 2.93％ 4.78％, 10.98％, 3.91％ and 4.83％, respectively. The crude protein contents of feathers (86.71％) and blood (82.99％) were higher than those of viscera (64.67％), feet (58.76％) and head (49.51％) , Inversely, the crude fat contents of blood (6.96％) and feathers (2.96％) were lower than those of head (26.19％), viscera (23.96％) and feet (13.73％). The crude ash contents of feet (21.69％) and head (20.38％) were higher than those of other by-products (0.96∼8.62％). The macro-mineral contents of head and feet were higher than those of other components, and the iron content of blood was higher than the other by-products. The total amino acid contents of poultry by-products showed the same trend as the crude protein contents. In addition, the Iysine content of feathers was very low compared to its high protein content. The essential amino acid contents of feathers were poorer than those of other offal components. Among the 5 offal components, the feathers showed the poorest amino acid availabilities. The ME contents were highest in viscera, and head, blood, feathers and feet. in decreasing order.