• Title/Summary/Keyword: 옥수수뿌리

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Isolation of Nitrogen-Fixing Bacteria from Gramineous Crops and Measurement of Nitrogenase Activity (벼과식물로부터 질소고정균의 분리와 Nitrogenase 활성 측정)

  • 최은화;이상은;윤기순;권덕기;손재근;박승환;한명숙;김사열
    • Microbiology and Biotechnology Letters
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    • v.31 no.1
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    • pp.18-24
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    • 2003
  • For researching nitrogen-fixing bacteria associated with gramineous crops, we collected growing roots of rices, wheats, oats, barleys, ryes, and maizes at 19 sites of southern Korean peninsula. Endophytes and free living bacteria were isolated from those crop roots. Sixty-three isolates were classified on the basis of different morphology, size, color, host of colony, and the 16S rDNAs sequence. The analyses of PCR amplification for nifH gene and nitrogenase activity assay, revealed that all isolates contained nitrogen-fixing abilities. In addition, most of them have cellulase activity which is one of the common features of endophytic bacteria from plant.

Farm Study of Direct Seeding Cultivation of the Korean Ginseng(Panax ginseng C. A. Meyer) (고려인삼의 직파재배에 관한 조사 연구)

  • Won, Jun-Yeon;Jo, Jae-Seong
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.4
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    • pp.308-313
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    • 1999
  • Direct seeding is a cultivation method of Korean ginseng which can cut down production cost and increase productivity. This study was conducted to obtain detailed information about this method and to aid the development of it in Korea. Major pre-crops before ginseng cultivation were rice, corn and rye, and organic matters, such as rice straw, rye straw and poultry feces were used for basal fertilization. In direct seeding of the ginseng field, seeding density was 196 to 210 seeds per $3.3m^2$ and germination ratio was 67%. Survival ratio of 4-year-old ginsengs which were products of direct seeding was 51%, and more than 100 plants per $3.3m^2$ survived. The most critical diseases in the directly seeded ginseng field were grey mold, damping off, and stem diseases including stem spot disease. Plant growth of the ginseng cultivated by the direct seeding method was not different from the ginseng cultivated by transplanting method. But the root of the ginseng cultivated by the direct seeding method took the shape of a shorter main root compared to the ginseng cultivated by the transplanting method. Root yield per $3.3m^2$ of the direct seeding ginseng field was larger compared to the field of the transplanting cultivation.

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Comparative Study of Infection Effects with AMF (Arbuscular-Mycorrhizal Fungi) Isolated from Upland Plants (밭 작물에서 분리한 균근균의 접종 효과 비교)

  • Kim, Seak-Jin;Kim, Ji-Hyun;Kim, Jin-Kyung;Chang, Kwang-Jin;Choi, Jang-Nam
    • Korean Journal of Plant Resources
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    • v.27 no.2
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    • pp.194-201
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    • 2014
  • This study was carried out to investigate the characteristics and infection effects of AMF with soil samples collected at some sites in Gyeonggi province, Korea. AMF spore and characteristics of infection structure in upland plant root were observed as wet sieving and staining method, growth of Capsicicum annum was compared between treatment and non treatment. AMF spores isolated from each soil sample were ellipse or circle type and the colors were soft yellow and white. The colonization rate of AMF with soil infection of Zea mays roots was 13.3~83.3%, the rate of infected soil collected from Z. mays was higher on average. When compared to the growth of C. Annum with control after infection on C. annum, it wasn't showing many differences in fresh weight, dry weight and height, but the yield of fruit of C. annuum showed double than the control group.

Allelopathic Effect of Sorghum Extract and Residues on Selected Crops and Weeds (수수의 타감작용(他感作用)에 관(關)한 연구(硏究))

  • Kim, S.Y.;De Datta, S.K.;Robles, R.P.;Kim, K.U.;Lee, S.C.;Shin, D.H.
    • Korean Journal of Weed Science
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    • v.14 no.1
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    • pp.34-41
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    • 1994
  • To better understand the allelopathic effect of sorghum(Sorghum vulgare L.), the inhibitory activities of water extracts of the stem, leaf and root, and of residues of the stem to major crops and weeds associated with them were evaluated. The allelopathic activity of sorghum plants was species specific, and depended on source and concentration. Germination, and shoot and root length of all test species were inhibited by the different concentrations of the stem extract. Among the crop species, radish showed the most inhibition, followed by wheat and rice. Maize was the least sensitive species. Of the weed species, Ipomoea triloba was most inhibited, followed by Echinochloa colona and Rottboellia cochinchinensis. The water extracts of leaves, stems, and roots significantly inhibited germination and seedling growth in E. colona and radish. The stem extract gave the greatest inhibitory effect on E. colona while all three extracts produced similar response in radish. In the greenhouse trial, sorghum stem residue placed on the soil surface as mulch significantly inhibited seedling growth in E. colona and radish, but not that in rice.

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Effects of Amendments on Ginseng Root Rot Caused by Fusarium solani Population Changes of the Microorganisms in Soil (토양(土壤)개량(改良)이 Fusarium solani 에 의한 인삼근부병과(人蔘根腐病)과 미생물(微生物) 변동(變動)에 미치는 효과(效果))

  • Son, Suh-Gyu;Shin, Hyun-Sung;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.13 no.1
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    • pp.41-47
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    • 1985
  • Eighteen plant residues were added to soil and the amended soil was inoculated with chlamydospores (5,000 cells/g soil) of Fusarium solani causing root rot of ginseng in soil to test the effects of amendment using pea (Pisium sativa L.) as an index plant. Distributional conditions of microogranisms in soil were compared with each other before and after dealing with soil amendment by plant debries. Infection rate by index plant's infectivity showed a higher degree in the treatment of wheat crushed than in control group, and followed by stalk of sweet potato, chinese cabbage, ginseng leaves and soybean pod. On the other hand, the de­creasing order of infection rate was root of garlic, welsh onion, cabbage leaf and stalk, green onion stalk, wheat straw and barley straw. In comparison with control group, the propagules of fungi increased in the treatment of ginseng leaves, soybean ground, wheat crushed, maize stalk, and chinese cabbage, but decreased in the root of garlic, cabbage, and barley straw. Population of total bacteria increased in the treatment of soybean ground, chinese cabbage, radish stalk, welsh onion, and wheat crushed, but decreased in barley straw, tobaco root, ginseng stalk, and wheat straw. The numbers of actinomycetes increased only in the treatment of soybean ground in a comparison with control and also decreased in the garlic stalk and tobaco root. The propagules of Fusarium spp increased in the treatment of chinese cabbage, welsh onion, radish stalk, wheat crushed, and sweet potato stalk, wheat crushed, and sweet potato stalk, but decreased in the treat­ment of wheat straw, ginseng leaves, and cabbage than control.

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A Study on Antioxidant Activity of Ethanol Extract from Rumex crispus and Metal Adsorptivity of it's Root (소리쟁이 에탄올 추출물의 항산화 활성 및 뿌리의 중금속 흡착능에 관한 연구)

  • Jeong, Kap-Seop
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.2
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    • pp.934-940
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    • 2012
  • Antioxidant activities such as reducing power, electron donating ability(EDA) and oxidation stability of Rumex crispus extracts with ethanol were examined, and adsorption of Pb(II) and Cd(II) with dried Rumex crispus powder in aqueous solution was experimented. Extraction yields based on the soluble solid contents ranged 1.9% to 3.0%, and were inversely proportional to solvent ratio but proportional to extraction temperature. Reducing power and EDA were also increased with extraction temperature but decreased with solvent ratio. These were corresponded to 86.4% and 91.0% of ascorbic acid reducing power and EDA, respectively, on 20 folds of solvent ratio and $75^{\circ}C$ Oxidation stability of Rumex crispus extracts on the corn seed oil was 86~91.3% of butylated hydroxytoluene. Pb(II) adsorptivity of Rumex crispus was higher than Cd(II), and adsorption rate of Pb(II) was 88% in 20 ppm concentration and 60min.

Synthesis of Strigol Analogues (Strigol 유사체의 합성)

  • Kim In Kyu;Hong Young Tech;Kim Sung Soo
    • Journal of the Korean Chemical Society
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    • v.36 no.5
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    • pp.764-769
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    • 1992
  • Strigol, a natural product isolated from root exudates of cotton, was found to be an extremely potent seed germination stimulant for the parasitic plant, witchweed. Witchweed seeds can remain dormant in the soil for several years until favorable conditions prevail including exposure to some type of chemical germination stimulant. Several strigol analogues, 3-methyl-4-(2-oxo-3,3a,6,6a-tetrahydro-2H-cyclopenta[b]furan-3-ylidenemethoxy)-but-2-en-4-olide(21), 4-(2-oxo-3,3a,6,6a-tetrahydro-2H-cyclopenta[b]furan-3-ylidenemethoxy)but-2-en-4-oilde(22), 3-methyl-4-(2-oxotetrahydrofuran-3-ylidenemethoxy)-but-2-en-4-olide(23), and 4-(2-oxotetrahydrofuran-3-ylidenemethoxy)-but-2-en-4-olide(24), were synthesized.

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The Effect of the Colonization of Serratia plymuthica A21-4 in Rhizosphere Soil and Root of Pepper in Different Soil Environment (근권토양의 환경이 고추역병 억제 미생물 Serratia plymuthica A21-4의 고추뿌리와 근권 토양 정착에 미치는 영향)

  • Cao, Pu;Shen, Shun-Shan;Wen, Cai-Yi;Song, Shuang;Park, Chang-Seuk
    • Research in Plant Disease
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    • v.15 no.2
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    • pp.101-105
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    • 2009
  • The biocontrol agent Serratia plymuthica A21-4 was selected and proved as an excellent inhibitor of Phytophthora blight of pepper through in vitro and in vivo experiments in previous studies. To enhance the colonizing density of S. plymuthica A21-4 on plant root and rhizosphere soil, some soil conditions might effect on the colonization of the bacteria were examined. The results obtained from the study indicated that the soils containing more sand were favorable to root colonization of S. plymuthica A21-4. Organic amendment such as 3% maize straw(w/w) was helpful to colonize the bacteria in root and soil. The soil temperature about $20^{\circ}C$, water content around 40%, and soil pH near to neutral or slightly acidic, were optimum condition for the colonization of S. plymuthica A21-4 in the rhizosphere soil and roots of pepper. In addition, existence of indigenous biotic entities was beneficial to the colonization of S. plymuthica A21-4.

Effect of Waterlogging on Growth and Morphological Characteristics of Roots of Maize Inbred Lines (담수 처리에 따른 옥수수 자식 계통의 지상부와 뿌리의 생육 및 형태적 특성)

  • Lee, Jihyun;Shin, Myeong-Na;Jung, Gun-Ho;Kim, Jung Tae;Cha, Jungeun;Shim, Kang-Bo;Lee, Jae-Eun;Son, Beom-Young;Kim, Sang-Gon;Ku, Bon-Il;Lee, Seukki;Jeon, Weon-Tai
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.4
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    • pp.227-235
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    • 2020
  • This study was conducted to investigate the growth characteristics of the shoot and roots and to analyse the morphological characteristics of roots of waterlogging resistant and susceptible maize inbred lines. Six maize inbred lines were treated with waterlogging for 10 days at V3, and the degree of leaf senescence was evaluated for waterlogging resistance. As a result of waterlogging resistance evaluation, KS85 was the most damaged inbred line with 3.33 senescence leaves and 5.54 degree, and KS141 was the least damaged inbred line with 1.33 senescence leaves and 3 degree. At 20 days after treatment, the effect of waterlogging stress on the shoot dry matter accumulation of KS85 and KS141 were decreased by 86.1% and 77.0%, respectively, compared to the control. Similarly, root dry matter accumulation of KS85 and KS141 were decreased by 77.6% and 65.0%. As a result of SEM photographs of the nodal roots of the two maize inbred lines, the thickness of cortex of KS141 was thicker than that of KS85, and the distortion of the cortex was observed in KS85 at 20 days after waterlogging. It was concluded that the thickness of cortex was related to maize waterlogging resistance.

Bioavailability Soil-aged Residues of the Herbicide Bentazon to Rice Plants (토양중(土壤中) 신생(新生) 및 숙성(熟成) Bentazon 잔유물(殘油物)의 벼에 의(依)한 흡수(吸收))

  • Lee, Jae-Koo;Kyung, Kee-Sung;Fuhr, F.
    • Applied Biological Chemistry
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    • v.32 no.4
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    • pp.393-400
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    • 1989
  • The amounts of $^{14}CO_2$ evolved during the $^{14}C-bentazon$ aging in soil for 3 and 6 months were 6.1 and 14.8% of the original radioactivity, respectively. The presence of earthworms in soil tended to increase the uptake of $^{14}C-bentazon$ by the roots of rice plants, even if it was not statistically significant. The evolution of $^{14}CO_2$ from $^{14}C-bentazon$ in soil increased in the presence of rice plants and earthworms compared with in the absence of them. The uptake of $^{14}C-bentazon$ residues by rice plants decreased remarkably with increasing the aging period within the limit of 3 months both in the absence and presence of earthworms, but there is not much difference between 3-month-aging and 6-month-aging. Much larger amounts of $^{14}C-labelled$ compounds were translocated to the shoots, compared with the data from a previous investigation using maize plants. The amount of non-extractable bound residue increased remarkably with the aging period up to 3 months. The polarity of the compounds extracted from soil increased with the aging and the growing of rice plants, indicating the formation of some polar metabolites.

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