• Journal of Veterinary Clinics
• /
• v.32 no.5
• /
• pp.417-421
• /
• 2015

Bone grafting is widely used to bridge major bone defects or to promote bone union. Natural calcium carbonate (CC) has been used as a bone substitute material and used to scaffold for bone morphogenetic protein (BMP). The aims of this study is to evaluate the biocompatibility of cuttlebone (CB) and hydroxyapatite from CB (CBHA). Each material was shaped into disks (5 mm in diameter and 2 mm in thickness). To test biocompatibility, the disks were implanted into the dorsal subcutaneous tissue in mice. Fibrous capsule thickness around each disk was evaluated histologically at 2 and 4 weeks after implantation. Concerning biocompatibility, fibrous capsule thickness of CBHA was significantly thinner than that of CB and CHA (p < 0.05) at 2 and 4 weeks after implantation. Based on the clinical and histological results, CBHA would be a safe material for use inside the body and has more effective osteoconduction than CB.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.29 no.4
• /
• pp.592-596
• /
• 2000

This study was carried out investigate the effect of sepiae as on the kimchi ferentation. Quality indices were pH, titratable acidity, reducing sugar content, microbial counts, and redness kimchi juice. Sepiae as retarded the decreasing rate of pH and the increasing rate of titratable acidity in kimchi during fermentation at 1$0^{\circ}C$, and the effect was more conspicouous at 1.5% than at 0.5%. Kimchi added with 1.5% sepiae as maintained a similar pH and titratable acidity after 5 days. Microbial counts of total and lactic bacteria were higher in sepiae os added kimchi than control control. Regardless of the of sepoae os, the redness of kimchi juice was gradually increased during storage.

• Journal of Veterinary Clinics
• /
• v.32 no.5
• /
• pp.410-416
• /
• 2015

Bone grafting is widely used to bridge major bone defects or to promote bone union. In the evaluation of bone defect regeneration, 5 mm-diameter defects were created in rabbit calvaria. Concerning biocompatibility, fibrous capsule thickness of CBHA (hydroxyapatite from cuttlebone) was significantly thinner than that of CB (cuttlebone) and CHA (hydroxyapatite from coral) (p < 0.05) at 2 and 4 weeks after implantation. Concerning 12-week total changes of radiologic gray-level histogram, CBHA was significantly higher than CHA (p < 0.05). In the evaluation of bone defect regeneration, bone formation of CHA was significantly higher than that of CB and CBHA (p < 0.05). Based on the clinical and histological results, CBHA would be a safe material for use inside the body and has more effective osteoconduction than CB. It is suggested that CBHA is a valuable bone graft material.