• Title/Summary/Keyword: 영지

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A new functional mushroom cultivated variety Jangsaeng of Fomitella fraxinea (기능성버섯 장수버섯의 신품종 『장생』)

  • Kong, Won-Sik;Yoo, Young-Bok;Jhune, Chang-Sung;You, Chang-Hyun;Cho, Yong-Hyun;Kim, Kwang-Ho
    • Journal of Mushroom
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    • v.3 no.4
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    • pp.129-132
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    • 2005
  • A new functional mushroom cultivated variety "Jangsaeng" of Fomitella fraxinea was developed by crossing monokaryons derived from collected strains for contribution to mushroom farmers. The major characteristics of this mushroom were compared to Yeongji 2ho of Ganoderma lucidum because of their morphological similarities. The optimal temperature of mycelial growth and fruitbody sprouting and growth were 30 and 25~30, respectively. The surface color of fruitbody pilus was reddish brown and the fruitbody was flat kidney-shaped like Ganoderma lucidum except that the stipe was absent. Its texture was so tough and rigid that the yield per 1,000 ml bottle might be as high as 136 g. The hot water extract of this medicinal mushroom is fit to drink just like Ganoderma lucidum but its taste is not bitter.

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Effect of Light on the Formation of Non-Basidiocarpous Basidiospores of Ganoderma Lucidum (영지(靈芝)의 비자실체성(非子實體性) 담자포자(擔子胞子)의 형성(形成)에 미치는 광(光)의 영향)

  • Shin, Gwan-Chull;Seo, Geon-Sik
    • The Korean Journal of Mycology
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    • v.17 no.4
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    • pp.189-193
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    • 1989
  • Ganoderma lucidum formed uniquely non-basidiocarpous basidiospore (NBB) from the aerial mycelia on agar media without the normal basidiocarps. Light was necessary for NBB formation. Best sporulation was obtained at the periodic exposure of 16 hour light and 8 hour darkness for more than 8 days. Effective wave length was blue and yellow but non sporulation was induced by near U.V. light. Effective light intensity for NBB bearing appeared to be about 1,000 Lux as white light. Photomorphogenetic responses in Ganoderma lucidum were clearly different among strains of the fungus.

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Spore germination and Sexuality of Ganoderma (영지(Ganoderma)의 포자 발아 및 Sexuality)

  • Kim, Kyung-Soo;You, Chang-Hyun;Cha, Dong-Yeul;Ko, Mi-Suk
    • The Korean Journal of Mycology
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    • v.26 no.1 s.84
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    • pp.16-19
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    • 1998
  • A study was carried out to explore the method for the germination of the spore of Ganoderma. Three strains of Ganoderma were examined using 2 different media such as PDA and LBA at three different temperatures. As it has been known the germination rate was very low under all the conditions examined. G. lucidum ASI 7004 showed 0.02% germination on both media at $25^{\circ}C$ and $30^{\circ}C$ respectively. The germination rate of ASI 7091 was 0.05% on PDA medium at $30^{\circ}C$. The germination rate of G. oregonense ASI 7067 was 0.67% on PDA at $35^{\circ}C$. LBA medium was found to be inadequate for the germination of Ganderma species in this study. In a consequent study, four mating types of G. oregonense such as $A_1B_1,\;A_1B_2,\;A_2B_1\;and\;A_2B_2$ were identified.

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Blockchain-based Personal Information Authentication Method using Zero Knowledge Proofs (영지식을 활용한 블록체인 기반 개인정보 인증 기법)

  • Lee, Kwang Kyu
    • Smart Media Journal
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    • v.10 no.3
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    • pp.48-53
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    • 2021
  • The authentication process is a key step that should be used to verify that a user is legitimate, and it should be used to verify that a user is a legitimate user and grant access only to that user. Recently, two-factor authentication and OTP schemes are used by most applications to add a layer of security to the login process and to address the vulnerability of using only one factor for authentication, but this method also allows access to user accounts without permission. This is a known security vulnerability. In this paper, we propose a Zero Knowledge Proofs (ZKP) personal information authentication scheme based on a Smart Contract of a block chain that authenticates users with minimal personal information exposure conditions. This has the advantage of providing many security technologies to the authentication process based on blockchain technology, and that personal information authentication can be performed more safely than the existing authentication method.

Input Certification protocol for Secure Computation

  • Myoungin Jeong
    • Journal of the Korea Society of Computer and Information
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    • v.29 no.8
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    • pp.103-112
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    • 2024
  • This study was initiated with the aim of authenticating that inputs have not been tampered with without disclosing them in the case of computations where multiple inputs are entered by participants using the same key. In general, in the authentication stage, authentication is performed after the input value is disclosed, but we do not want to reveal the inputs until the end. This is a case of deviating from the traditional security model in which malicious participants exist in cryptography, but it is a malicious attack method that can actually occur enough. Privacy infringement or distortion of calculation results can occur due to malicious manipulation of input values. To prevent this, this study studied a method that can authenticate that the message is not a modified message without disclosing the message using the signature system, zero-knowledge proof, and commitment scheme. In particular, by modifying the ElGamal signature system and combining it with the commitment scheme and zero-knowledge proof, we designed and proved a verification protocol that the input data is not a modified data, and the efficiency was improved by applying batch verification between authentication.

Inhibitory Activity of Edible Mushrooms on the Tissue Thromboplastin (Tissue Factor) (조직 혈액응고인자에 대한 식용버섯류의 저해활성)

  • Hwang, Keum-Hee;Kim, Hyun-Ku;Han, Yong-Nam
    • Korean Journal of Food Science and Technology
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    • v.29 no.1
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    • pp.161-166
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    • 1997
  • Tissue thromboplastin (tissue factor), a membrane bound glycoprotein is an important initiating factor in blood coagulation cascade, which leads to the formation of thrombin by activating both factor X and IX. Activation of blood coagulation by TF is essential for blood injury, and stimulates the blood coagulation in myocardial infarction, cancer and blood coagulatory diseases. High density lipoprotein, apolipoprotein A-II were known to be biological TF inhibitors. Recently, studies on search for TF inhibitors from natural products have been active in Korea. Among the edible mushrooms screened for inhibitory activities on the TF, Lentinus edodes showed the most strong activity, followd by Agaricus bisporus and Ganoderma lucidium. And the fractionation of the above mushrooms with the chloroform ($CHCl_3$) and ethylacetate (EtOAc) was done and evaluated for the inhibitory activities on TF. In Ganoderma lucidium, $CHCl_3$ fraction and $H_2O$ layer were not active, but EtOAc fraction exhibited a strong inhibitory activity on TF and the $IC_{50}$ value was $1.07{\times}10^{-4}\;g$. In the case of Agaricus bisporus, there were no inhibitory activities on the TF in all of the fractions. $CHCl_3$ fraction and $H_2O$ layer of Lentinus edodes did not show inhibition on the TF but EtOAc fraction showed strong inhibition on the TF, and the $IC_{50}$ value was $7.70{\times}10^{-4}\;g$.

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Studies on Constituents of Higher Fungi of Korea(LVI) -General Constituents and lmmunostimulation of Ganoderma lucidum- (한국산(韓國産) 고등(高等) 균류(菌類)의 성분(成分) 연구(硏究)(제(第)56보(報)) -영지(靈芝)의 성분(成分) 및 면역촉진작용(免疫促進作用)-)

  • Lee, Myung-Hee;Kim, Ha-Won;Shim, Mi-Ja;Toh, Sang-Hak;Choi, Eung-Chil;Kim, Byong-Kak
    • The Korean Journal of Mycology
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    • v.14 no.2
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    • pp.149-163
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    • 1986
  • To determine contents of general constituents of Ganoderma lucidum in Korea, the dried carpophores were analyzed. The contents of water, ash, crude lipid, crude protein and crude cellulose were 14.6, 2.0, 3.3, 23.6, and 59.0%, respectively. Among reducing sugars, maltose was the most abundant. Seventeen free amino acids were detected, showing alanine the highest value. The pH of hot water extract was 4.1-4.2. The spores of Ganoderma lucidum was flat and ovoidal long. Their size was $6.3-7.1{\times}3.5-4.3{\times}2.0-2.5\;{\mu}m$ long. To examine effects of life span against sarcoma-180 cells, Fractions A, B and C, were obtained from the extract of Ganoderma lucidum. The survival rates of Fractions A, B and C were 131.7, 162.5, and 141.7 %, respectively. In addition, to examine effects of Fraction B on cell-mediated immunity, delayed type hypersensitivity (=DTH) test was conducted. It restored the suppressed DTH in the sarcoma-180 bearing group up to 66.7%.

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Effect of Fermented Ginseng Extract by Mushroom Mycelia on Antiproliferation of Cancer Cells (버섯균사체로 발효시킨 인삼 추출물의 암세포 증식억제 효과)

  • Kim, Hyun-Young;Joung, Eun-Mi;Hwang, In-Guk;Jeong, Jae-Hyun;Yu, Kwang-Won;Lee, Jun-Soo;Jeong, Heon-Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.1
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    • pp.36-41
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    • 2010
  • This study was conducted to investigate the effects of fermented ginseng extract by mushroom mycelia on antiproliferation of cancer cells. Phellinus linteus, Ganoderma lucidum, and Hericium erinaceum mycelia were inoculated to ginseng. The effects of fermented ginseng extract on antiproliferation of stomach (MKN-45), colon (HCT116), mammary (MCF-7), lung (NCIH460), prostate (PC-3), and liver (HepG2) cancer cells were investigated by MTT assay. Fermented ginseng extract showed significant antiproliferation effects compared with fresh ginseng extract. Fermented ginseng extract by P. linteus, G. lucidum, and H. erinaceum mycelia showed growth-inhibitory effect of 44.50, 17.75 and 43.98% viability at 1.5 mg/mL on the MKN-45 cell line, 62.86, 3.73, and 54.55% at 1.5 mg/mL on the HCT116 cell line, 41.81, 7.01, and 37.84% at 1.5 mg/mL on the MCF-7 cell line, 53.52, 5.31, and 35.27% at 1.5 mg/mL on the NCIH460 cell line, 35.05, 3.07, and 44.29% at 1.5 mg/mL on the PC-3 cell line, and 59.57, 6.34, and 4.97% at 1.5 mg/mL on the HepG2 cell line, respectively. These results indicated that fermented ginseng by G. lucidum mycelium showed the highest antiproliferation effect against various cancer cells.

Grouping of Ganoderma strains based on cultural characteristics and fruitbody morphology (영지버섯 수집균의 배양적 특성 및 자실체 형태에 따른 구분)

  • Kim, Kyung-Soo;Kong, Won-Sik;Choi, Sun-Gyu;You, Chang-Hyun;Ko, Mi-Suk;Seo, Geon Sik
    • Journal of Mushroom
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    • v.2 no.2
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    • pp.49-59
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    • 2004
  • To establish a genetic relationships of collected Ganoderma strains, mycelium growth according to media and temperature, colony morphology, chlamydospore formation and fruitbody morphology were investigated. For the identification of optimal growth conditions of the strains, five different growth media and four different temperature were tested. GCM (Ganoderma complete medium) at $30^{\circ}C$ was the most effective for mycelial growth of 68 strains with more or less variation. The strains were divided into 28 groups based on their colony shapes, and most of them belong to CM3 or CM8 group. Chlamydospores were observed in the mycelia of 16 strains including ASI 7022 on microscope, but not in most G. lucidum domestic strains, which showed relatively lagging growth on $35^{\circ}C$ in mycelial growth experiment. These results were not similar to those of G. lucidum but those of G. tsugae imported from USA. The strains were cultivated on oak sawdust media to see their fruit body formation. Ninety-seven among 115 strains formed fruitbodies in sawdust cultivation. They showed two forms of fruitbodies, 89.7% of flat type or 10.3% of antler type, although these shapes can be affected by $CO_2$ concentrations. These results suggest that the native strains formerly considered to belong to G. lucidum have to be re-classified with further study.

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Effect of Far-infrared Irradiation on the Antioxidant Activity of Extracts from Phellinus igniarius and Ganoderma lucidum (원적외선 처리가 상황버섯과 영지버섯 추출물의 항산화능에 미치는 영향)

  • Lee, Seung-Cheol;Ju, Young-Cheoul;Kim, Jeong-Han
    • Korean Journal of Food Science and Technology
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    • v.39 no.4
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    • pp.386-389
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    • 2007
  • The antioxidant activities of Phellinus igniarius (PI) and Ganoderma lucidum (GL) extracts were evaluated after far-infrared (FIR) irradiation. PI and GL were irradiated at $120^{\circ}C$ for 30, 60, 90 and 120 min with a FIR heater (2-14 ${\mu}m$), and then extracted by distilled water for 1 hr. Total sugar contents (TSC), total phenolic contents (TPC), radical scavenging ability (RSA), and reducing power (RP) were significantly increased by FIR irradiation. When PI was irradiated for 120 min, the TSC, TPC, RSA, and RP values of the PI extracts increased from 10.1 to 25.9 mg/g, 6.1 to 13.4 mg/g, 23.8 to 51.5%, and 0.222 to 0.363, respectively, compared to the untreated controls. The GL extracts, under the same conditions ($120^{\circ}C$ for 120 min), also increased in TSC, TPC, RSA, and RP from 11.1 to 22.6 mg/g, 2.5 to 5.8 mg/g, 14.5 to 18.6%, and 0.271 to 0.296, respectively. These results indicate that appropriate FIR irradiation of PI and GL could enhance the antioxidant activities of their extracts by increasing amounts of phenolic and sugar compounds.