• Current Research on Agriculture and Life Sciences
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• v.4
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• pp.42-49
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• 1986

The changes in pectinesterase (PE) and polygalacturonase (PG) activities were investigated during maturation and ripening of tomato fruits. PG was isolated from ripe tomatoes and purified by using DEAE-sephadex A-50 column chromatography. Its property was examined by general methods. PE activity was increased during maturation and then peaked at the on set of color change. PG activity was not detectable in mature green tomato fruits but increased during softening. Slab electrophoresis of the crude enzyme isolated from ripe tomatoes shoed 6 bands. Two polygalacturonase (PGI, PGII) were separated from crude enzyme of ripe tomatoes by chromatography on DEAE-sephadex A-50. PGI and PGII were purified by 61 fold and 98 fold by the present procedure, respectively. The Rm values of partially purified PGI and PGII were estimated to be 0.25 and 0.31, respectively. The optimum temp, and pH of PGI activity were $37^{\circ}C$ and pH 4.5, while those of PGII activity were $40^{\circ}C$ and pH 4.7, respectively. Isozyme PGI activity was the most stable at pH 4.3 and retained 50% of its activity when exposed at $78^{\circ}C$ for 5 min.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.1
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• pp.16-24
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• 1998

To determine the optimum application of fertilizers for the cultivation of Chinese cabbage in plastic film house, twenty soils which contain different salts contents were taken from 4 different area of plastic film house cultivation, Youngdong. Boeun county, Cheongweon county, and Cheongju city. The dry weight and the amount of N. P, and K uptakes of Chinese cabbage in the plot of no fertilization were considered as the factors representing the fertility of the soil. And a difference of dry weight and the amounts of N, P, and K uptakes of plants between the plot of fertilization and no fertilization were considered as the factors representing the total effect of fertilizer and fertilizer N, P, and K effects. respectively. These factors of soil fertility and fertilizer effects were estimated by correlation and regression with soil tests in order to find the critical levels and recommended method for optimum fertilization of Chinese cabbage. Chinese cabbage transplanted in two soils, having the electrical conductivity of 9.3 and 15.2 dS/m, were not able to root due to the salts toxicity. The content of inorganic N, the electrical conductivity, and CEC were founded to have significant correlation with the factors of both the soil fertility and fertilizer effects for the cultivation of Chinese cabbage. To determine the weighting degree for the productivity and the fertilizer effects, the standardized partial regression coefficient was analyzed by regression among the factors of fertility, the fertilizer effects, and the soil tests. The coefficient for inorganic N($NH_4-N$ and $NO_3-N$) was obtained as the absolute value of 756-1871 and this value was extremely higher than those of other soil tests which was 0.07-4.11. These results suggested that the content of inorganic N is the best tests for the estimation of the productivity and the fertilizer effects for the cultivation of Chinese cabbage in plastic film house. The critical level of inorganic N($NH_4-N+NO_3-N$) estimated by Cate-Nelson split method for maximum productivity and zero point of fertilizer effect was 220 mg/kg for all the factors of estimation. These results suggested that no application of fertilizer N. P, and K is required at the critical level of inorganic N of soil. Consequently the optimum application of fertilizer N, P, and K for the cultivation of Chinese cabbage in plastic film house was possible to determine by the critical level of inorganic N of soil. The critical level of electrical conductivity was estimated as 2.8 dS/m by the same method.

• Journal of Environmental Impact Assessment
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• v.27 no.6
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• pp.604-617
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• 2018

As the depletion of forests became more widespread due to the increase in the number of roads, the research was conducted on the relationship between temperature and humidity in the forests, assuming that the forests around the roads were affected. Through the forest monitoring, the temperature and humidity of coniferous forests and broadleaf forests in Sedong and Gongju areas were observed at three point of 10m, 20m and 30m from the road boundary to the inside of the forest, respectively. In Yeongdong area, for more reliable results, it was observed from the point of 0m, 10m, and 20m. During the study period, so it was expected the change in tree growth was small, the change of temperature and humidity inside the forest by the road was compared with the temperature and humidity from the road to the inside of the forest from September 2017 to January 2018, the changes of temperature and humidity inside the forest due to linear development such as roads were quantitatively analyzed. Using the HOBO data logger (MX2301, Onset Corp.), the temperature and humidity changes of each site were measured, and the average of the changes have been analyzed monthly. In the case of Gongju coniferous forests in September 2017, the average weekly temperature is $0.57^{\circ}C$ higher than the forest outside from the forest boundary and $1.23^{\circ}C$ higher than the inside of the forest, at night in November 2017, in Sedong broadleaf forests. That is, the ability to control the temperature and humidity of the forests along the road was larger and less variable as the distance from the road boundary to the inside of the forest increased. In this study, it is considered that the high degree of change in temperature and humidity of the forest and the surrounding area due to artificial linear development such as roads will affect the growth of trees. This results could serve as a basis for studying the quantitative scope of linear development affecting forest growth and for managing forest change caused by linear development.

• Korean Journal of Environment and Ecology
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• v.35 no.5
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• pp.548-557
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• 2021

Cypripedium japonicum Thunb. is an endemic plant in East Asia, distributed only in Korea, China, and Japan. At the global level, the IUCN Red List evaluates it as "Endangered Species (EN)," and at the national level in Korea, it is evaluated as "Critically Endangered Species (CR)." In this study, we investigated the characteristics of the age structure and the sustainability of the population based on the data obtained by demographic monitoring conducted for seven years in the natural habitat. C. japonicum habitats were observed in 7 regions of Korea (Pochoen, Gapyeong, Hwacheon, Chuncheon, Yeongdong, Muju, Gwangyang), and 4,356 individuals in 15 subpopulations were identified. The population size and structure differed from region to region, and artificial management had a very important effect on the size and structural change of the population. Population viability analysis (PVA) based on changes in the number of individuals of C. japonicum showed a very diverse tendency by region. And the probability of population extinction in the next 100 years was 0.00% for Pocheon, 10.90% for Gwangyang, 24.05% for Chuncheon, and 79.50% for Hwacheon. Since the above monitored study sites were located within the conservation shelters, which restricted access by humans, unauthorized collection of C. japonicum, the biggest threat to the species, was not reflected in the individual viability. So, the risk of extinction in Korea is expected to be significantly higher than that estimated in this study. Therefore, it is necessary to reflect population information in several regions that may represent various threats to determine the extinction risk of the C. japonicum population objectively. In the future, we should expand the demographic monitoring of the C. japonicum population known in Korea.

• Research in Plant Disease
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• v.26 no.4
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• pp.264-271
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• 2020

We have developed a simultaneous diagnostic method that can identify both the species of thrips and tomato spotted wilt virus (TSWV) that are problematic in chrysanthemum plants. This is a method of amplifying DNA by performing reverse transcription-polymerase chain reaction by simultaneously adding primers specific to TSWV coat protein (N) gene and primers specific to the internal transcribed spacer 2 region of Frankliniella occidentalis and F. intonsa using total nucleic acid extracted from one thrips. The sizes of DNA fragments for TSWV, F. occidentalis, and F. intonsa were 777, 287, and 367 bp, respectively. These results showed species identification of thrips and whether thrips carrying TSWV can be simultaneously confirmed. Further usefulness of the simultaneous diagnostic method was made from greenhouse survey at chrysanthemum greenhouses in Taean (Chungcheongnam-do) and Changwon (Gyeongsangnam-do) to investigate the identification of thrips species and the rate of thrips carrying TSWV. Of thrips collected from the greenhouses, 83.7% thrips was F. occidentalis and 72.9% F. occidentalis carried TSWV in Taean. Similarly, the diagnostic method showed that 92.2% thrips was F. occidentalis and 84.0% F. occidentalis carried TSWV in Changwon. These results confirm that F. occidentalis is a dominant thrips species and the thrips species plays a crucial role in the transmission of TSWV in chrysanthemum plants in the greenhouses. Taken together, this study showed a simple diagnostic method for thrips identification and epidemiological studies of the timing and spread of TSWV through thrips in chrysanthemum greenhouses in South Korea.

• Korean Journal of Environment and Ecology
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• v.37 no.4
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• pp.289-301
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• 2023

Forests within watersheds are essential in maintaining ecosystems and are the central infrastructure for constructing an ecological network system. However, due to indiscriminate development projects carried out over past decades, forest fragmentation and land use changes have accelerated, and their original functions have been lost. Since a forest's structural pattern directly impacts ecological processes and functions in understanding forest ecosystems, identifying and analyzing change patterns is essential. Therefore, this study analyzed structural changes in the forest landscape according to the time-series land cover changes using the FRAGSTATS model for the dam watershed of the Geum River upstream. Land cover changes in the dam watershed of the Geum River upstream through land cover change detection showed an increase of 33.12 square kilometers (0.62%) of forests and 67.26 square kilometers (1.26%) of urbanized dry areas and a decrease of 148.25 square kilometers (2.79%) in agricultural areas from the 1980s to the 2010s. The results of no-sampling forest landscape analysis within the watershed indicated landscape percentage (PLAND), area-weighted proximity index (CONTIG_AM), average central area (CORE_MN), and adjacency index (PLADJ) increased, and the number of patches (NP), landscape shape index (LSI), and cohesion index (COHESION) decreased. Identification of structural change patterns through a moving window analysis showed the forest landscape in Sangju City, Gyeongsangbuk Province, Boeun County in Chungcheongbuk Province, and Jinan Province in Jeollabuk Province was relatively well preserved, but fragmentation was ongoing at the border between Okcheon County in Chungcheongbuk Province, Yeongdong and Geumsan Counties in Chungcheongnam Province, and the forest landscape in areas adjacent to Muju and Jangsu Counties in Jeollabuk Province. The results indicate that it is necessary to establish afforestation projects for fragmented areas when preparing a future regional forest management strategy. This study derived areas where fragmentation of forest landscapes is expected and the results may be used as basic data for assessing the health of watershed forests and establishing management plans.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.388-397
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• 1985

Forage productivity and sequential changes in forage quality of four rye and two triticale varieties were studied. All the observed characteristics were similar among rye or triticale varieties, but they were different between rye and triticale varieties. Early growth of rye was better compared to triticale varieties, but at heading stage yield in dry weight, digestible dry matter and percent dry matter of triticale were higher compared to rye varieties. Heading stage of rye was earlier than that of triticale by 11 - 14 days. Triticale had longer and broader leaf blades and a higher leaf blade/total dry weight ratio compared to rye. Protein content decreased as plant growth advanced and it was higher in rye before heading, but it was similar after heading compared to triticale. Cellulose and lignin contents of rye increased through 20 days after heading. However, in triticale cellulose increased until heading stage and it levelled off, but lignin content increased rapidly after heading. Among the crude fibers, only acid detergent fibers(ADF) was negatively correlated with in vitro dry matter digestibility(IVDMD) in both rye and triticale. IVDMD of rye decreased rapidly after heading, but it was maintained as high as heading stage up to 15 days after heading in triticale. Protein content and IVDMD in leaf blades were higher than those of culm + leaf sheath, hemicellulose was similar, and cellulose, lignin, and ADF in leaf blades were lower compared to culm + leaf sheath. In the early and middle part of April rye was superior to triticale as a soiling crop because of a better plant growth of rye under the low temperature conditions, but in the later part of April and early part of May triticale was superior to rye because of a rapid decreased in IVDMD of rye after heading and a late maturing characteristics of triticale. Planting both rye and triticale could prolong the utilization period of the soiling crops and increase in yield with better quality compared to a single crop of rye or triticale.

• Korean Journal of Environment and Ecology
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• v.36 no.6
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• pp.639-650
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• 2022

Abeliophyllum distichum Nakai, a rare plant with distylous characteristics, is native to certain parts of the Korean Peninsula. It is registered on the IUCN Red List of Threatened Species as a globally endangered plant. This study was conducted to establish an appropriate local conservation management plan suitable for future A. distichum populations by comparing and analyzing the flowering characteristics and population size according to distyly based on the results of quantitative surveys in 14 regions, including 8 areas with native populations of A. distichum and 6 natural monument populations. The number of individuals appearing in each population group was surveyed, and the flowering individuals were identified by style as being either pin or thrum flower types as they were being examined and recorded on the site. In total, 13,130 individuals of A. distichum (7,003 flowering and 6,127 non-flowering individuals) were recorded, but the balance of the number of pin- and thrum-flowered individuals in each population was not significant (p<0.05), indicating an imbalanced state. In particular, the Yeongdong (YD) population was very disproportionate compared to other populations, suggesting that its genetic diversity was low and the possibility of inbreeding was high. The average flowering and fruiting rates by management unit were much higher in the natural monument populations (89.2% and 55.3%, respectively) than in the natural habitat populations (39.0% and 8.5%, respectively). It may be due to a difference in reproductive growth resulting from light inflow into the forest caused by the upper crown closure. The area of occupation (AOO) of A. distichum on the Korean Peninsula covered an area of 23,224.5 m². Although the natural monument population was smaller than the natural habitat population, its density was higher, likely as a result of the periodic management of natural monument populations, where the installation of protective facilities in certain areas restricts population spread. Conservation of A. distichum populations requires removing the natural monument populations suspected of anthropogenic and genetic disturbances and expanding the conservation priority population by designating new protected areas. Although the habitats of natural monument populations are managed by the Cultural Heritage Administration and local governments, there are no agencies that are responsible for managing natural habitat populations. Therefore, institutional improvement in the overall management of A. distichum should be prioritized.

• Journal of the Korean Society of International Agriculture
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• v.23 no.5
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• pp.537-545
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• 2011

This study was conducted to obtain basic information on physiological and proteomic responses of barley seedlings to salt stress. Shoot dry weight decreased significantly as the level of soil salinity increased. Salt stress-induced decrease of relative shoot dry weight was lower in cv. "Sanglok" than in cv. "Sunwoo". Under the salt stress, SPAD value decreased, and the value was higher in cv. "Sanglok" than in cv. "Sunwoo". Sodium ion content in the leaves increased as NaCl concentration increased, and the content was higher in cv. "Sunwoo" than in cv. "Sanglok". The K⁺/Na⁺ ratio was higher in cv. "Sanglok" than in cv. "Sunwoo". Salt stress-induced alterations in protein expression of the leaves were detected by two dimensional electrophoresis, and 47 protein spots showing altered expression were selected. Among the selected protein spots, 17 protein spots were up-regulated and 28 spots down-regulated in cv. "Sanglok". In cv. "Sunwoo", 14 protein spots were up-regulated and 27 spots down-regulated. Out of 47 deferentially expressed protein spots, 18 protein spots were identified using mass spectrometry and NCBI protein database. Among the identified proteins, ten proteins are known to be involved in various stress responses, but the others are not directly involved in stress responses.

• Journal of Plant Biotechnology
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• v.50
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• pp.1-10
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• 2023

Sweet potato (Ipomoea batatas L. Lam) is an economically important root crop and a valuable source of nutrients, processed foods, animal feeds, and pigment materials. However, during post-harvest storage, storage roots of sweet potatoes are susceptible to decay caused by various microorganisms and diseases. Post-harvest curing is the most effective means of healing wounds and preventing spoilage by microorganisms during storage. In this study, we aimed to identify proteins involved in the molecular mechanisms related to curing and study proteomic changes during the post-curing storage period. For this purpose, changes in protein spots were analyzed through 2D-electrophoresis after treatment at 33℃ (curing) and 15℃ (control) for three days, followed by a storage period of eight weeks. As a result, we observed 31 differentially expressed protein spots between curing and control groups, among which 15 were identified. Among the identified proteins, the expression level of 'alpha-amylase (spot 1)' increased only after the curing treatment, whereas the expression levels of 'probable aldo-keto reductase 2-like (spot 3)' and 'hypothetical protein CHGG_01724 (spot 4)' increased in both the curing and control groups. However, the expression level of 'sporamin A (spot 10)' decreased in both the curing and control treatments. In the control treatment, the expression level of 'enolase (spot 14)' increased, but the expression levels of 'chain A of actinidin-E-64 complex+ (spot 19)', 'ascorbate peroxidase (spot 22)', and several 'sporamin proteins (spot 20, 21, 23, 24, 27, 29, 30, and 31)' decreased. These results are expected to help identify proteins related to the curing process in sweet potato storage roots, understand the mechanisms related to disease resistance during post-harvest storage, and derive candidate genes to develop new varieties with improved low-temperature storage capabilities in the future.