• Korean Journal of Environmental Agriculture
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• v.13 no.2
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• pp.131-141
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• 1994

To investigate differences in Cd and Zn contents in paddy soils and rice plants polluted by aerial emissions from the Janghang smelter, soil samples in the different directions and at the surface (0-15cm) and subsurface (15-30cm) in 1982 and 1990, and rice plants at the corresponding sampling sites in 1990 were collected from the Janghang Smelter Area. Soil samples were extracted with $4M-HNO_3$ and plant samples were digested with a mixture of $HNO_3$ and $HClO_4$for analyzing by atomic absorption spectrophotometry. The Cd and Zn contents in soils ranged from 0.09 to 4.42 and from 16.0 to 959.5mg $kg^{-1}$, respectively. The average contents of Cd and Zn in 1990 were higher than those in 1982. The Cd and Zn contents of soils near the center of the smelter were higher than those of soils farther from the center and also decreased in the order of east > north-north east > north east > north. The Cd and Zn levels in surface soils were higher than those in subsurface soils. The contaminated areas of Cd and Zn were within 4km in the east, and within 3km in the north-north east and the north east. Metal contents in brawn rice were the lowest in rice plants. The Cd content of brown rice was one sixth of that in leaf blade and in leaf sheath. The Cd content of leaf blade, stem and panicle axis were significantly correlated with the levels of Zn, Cu and Pb in soils, and Zn content of stem was significantly correlated with the levels of Cu and Pb. The Cd and Zn content in brown rice ranged from 0.05 to 0.25mg $kg^{-1}$ and from 10.5 to 30.9㎎ $kg^{-1}$ in the smelter area, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.1
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• pp.10-19
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• 1993

This study reports the influence of nitrogen application on the yield and the accumulation of $NO_3{^-}$ in edible parts of major vegetables in a pot experiment treated with five levels(0, 1, 2, 4, 8g N/pot) of nitrogen. In the $NO_3{^-}$ accumulation of chinese cabbage the outer leaf were superior to the inner leaf. The $NO_3{^-}$ contents of the midrib in outer and inner leaf were higher than those of the leaf blade. By increasing the nitrogen application, the accumulationsgap in $NO_3{^-}$ accumulation between midrib and leaf blade in a leaf, became larger. The difference ratio of $NO_3{^-}$ accumulation in the outer leaf reached 4.8:1 at 8g N/pot treatment. In theradish, the $NO_3{^-}$ accumulation in the aerial root parts is higher than those of the underground root parts, and it is higher in the leaf than in the petiole. The accumulation of $NO_3{^-}$ in sarcocarp of cucumber was increased along with the added amount of nitrogen, but contents of $NO_3{^-}$ in the core of the cucumber showed no differences in the treatment levels. The $NO_3{^-}$ accumulation differences of outer sarcocarp vs. inner core parts in cucumber was increased along with the higher nitrogen levels, and its difference ratio of $NO_3{^-}$ accumulation reached 13.1:1 at 8g N/pot treatment. The highest $NO_3{^-}$ accumulation in edible parts of chinese cabbage, radish and cucumber were found at the 8g N/pot treatment, and were 3,664ppm in the outer leaf midrib of chinese cabbage, 3,449ppm in the aerial part of root of radish, and 484ppm in sarcocarp part of cucumber. Compared with the control each 130 times, 40.8 times, 20.9 times, respectively. There are positive correlation coefficients between the amount of nitrogen fertilization, $NO_3{^-}$ accumulation in the edible parts, yield, and yield components of edible parts.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.3
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• pp.375-382
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• 1986

This study was carried out to obtain the basic information for the clarification of spring growth habits mechanism of naked barleys. The isozyme patterns and activities of peroxidase in the young spike and leaf blade were analyzed during the differentiation and development of young spike. The characteristic differences between the normal and rosetted type were in c and g isozymes in young spike, and in i isozyme in the leaf blade. In the normal type, c and i isozymes disappeared at the stage of spi-kelet differentiation, g isozyme at the stage of flolet differentiation. But, in the rosetted type, those three isozymes remained in dark stained condition until the time of final sampling. Especially, those three isozymes were higher in the rosetted type than those in the normal type even at the stage of bract differentiation(BDS), just prior to the reproductive stage. The activities of peroxidase decreased slowly after BDS in the young spike and leaf blade in the normal type, While, in the rosetted type, increased linearly, and the degree of increasing was remarkable in the young spike. It was interesting that the degree of activities in young spike was higher in the rosetted type than that in the normal type even at BDS. From the above results, the remarkable differences of the isozyme patterns and activities at BDS between the normal and rosetted type were considered to be the physiological expression of the varieties concerned with the degree of spring growth habits.

• Applied Microscopy
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• v.38 no.1
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• pp.21-28
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• 2008

Carnivorous plants vary in their unique features of morphology, ultrastructure and biochemical properties by species. Furthermore, prey-capturing mechanism as well as structural and physiological adaptations have been used for grouping various carnivorous species. In Drosera plants, glandular trichomes, which develop in the leaf epidermis, are known to play the most important role during the prey capturing process. The present study examined such trichomes, focusing on the glandular type, in leaves of Drosera anglica using scanning and transmission electron microscopy. Three types of rudimentary glandular trichomes were found to develop within the folded leaf primordia and immature leaf during early development. The first type, stalked glandular trichomes (Type I), occurred on the margin and upper epidermis of the leaf. With maturation, the longest glandular trichomes having lengthy stalks, ca. $2.2{\sim}5.1\;mm$, developed along the margin, while shorter stalked trichomes, ca. up to $200\;{\mu}m$, were found on the inner leaf blade. The shorter ones consisted of a globose head having two layers of secretory cells, parenchyma bell cells and tracheids and a multicellular stalk. The stalks gradually decreased in length in centripetal fashion. The second type, Type II, having ca. $15{\sim}30\;{\mu}m$ short stalks, also developed along the inner blade. Both types secreted mucilage from the secretory cells which had a thin cell wall and cuticle layer. The sessile six-celled glandular trichomes were the third type, Type III, and were $25{\sim}40\;{\mu}m$ in length. They were distributed most commonly throughout the upper and lower epidermis, petiole and even on the stalk surfaces of the first two types of trichomes. The third type was also found to be involved in the active secretion. In prey capturing leaves, all trichome types secreted substances through thin cuticles in the head cell wall, which exhibited relatively loose wall components.

• Korean Journal of Weed Science
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• v.9 no.2
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• pp.108-115
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• 1989

An experiment was carried out at the International Rice Research Institute in 1987 to understand the seed germination and seedling development of rice and Echinochloa species. The percentage germination of rice cultivars(IR64 for lowland-type and UPLRi-5 for upland-type) was extremely high(>90%) regardless of temperature while that of Echinochloa species ranged from 10 to 80% depending on the species. Among these, E. crus-galli ssp. kispidula had the lowest germination with less than 20% at the high temperatures ($30^{\circ}C$, $35^{\circ}C$) and about 45% at the low temperature ($25^{\circ}C$). Original seed weight gradually decreased with time white root and shoot weight increased for both rice and Echinochloa species. However, increase in root and shoot weight did not compensate for the loss of stored carbohydrate during the experimental period. The root of E. glabrescens grew from the seed on the opposite side of the shoot while for rice it grew from the same side of the seed as the shoot. Echinochloa glabrescens had a leaf blade-like expanded first leaf that contained chlorophyll while rice had an incomplete first leaf that had no leaf blade and no chlorophyll. Due to this E. glabrescens could grow independently 2 to 3 days earlier than rice.

• Applied Microscopy
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• v.39 no.1
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• pp.57-64
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• 2009

The trapping leaves of Drosera capture insects by secreting sticky mucilage from numerous glandular trichomes (GTs) that are developed on the leaf epidermis. The present study examines and compares the structural features of those trichomes in Drosera binata and D. pygmy with the use of light and electron microscopy. The study focuses primarily on the development and differentiation pattern of the GTs during growth. Upon examination, the upper and lower epidermis were readily distinguishable by the features of GTs in developing leaves. In particular, the GTs were dense in the upper epidermis and along the leaf margin. In D. binata, the capitate GTs with elongated stalk and sessile peltate GTs were found most commonly, whereas only capitate GTs with varying degrees of the stalk length were observed in D. pygmy. Up to ca. $2.2{\sim}3.4\;mm$ long capitate GTs were seen in the leaf margins of D. binata and ca. $3.7{\sim}4.2\;mm$ long GTs having racket-like head with adaxial hemispheric structures, otherwise known as tentacles, were noted in the leaf margin of D. pygmy. The peltate GTs were found to be distributed in the lower epidermis of D. binata. In both species, head cells were dense with cytoplasm containing high numbers of Golgi bodies, ER, mitochondria and small vesicles. Secretory materials accumulated within numerous small vacuoles, then fused together to form a single large vacuole, which serves as a secretory cavity. Flection movement of the marginal GTs and leaf blade GTs, and increased mucilage secretion from the head cells upon contact with prey during the capturing process are considered to be major factors in their active insectivorous mechanism. The findings of this study will be useful in comparisons to similar findings in other species that form adhesive trapping leaves, such as Drosophyllum or Pinguicula., further contributing a better understanding of the function and structure of the trapping leaves of carnivorous plants.

• Korean Journal of Weed Science
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• v.14 no.2
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• pp.128-143
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• 1994

At 5 DAS/T, leaf primordia of rice stems that were grown under dry condition in transverse sections were strongly stained while those under water condition had many aerenchyma cells well developed. On the other hand, leaf primordia and large air spaces in stem of transplanted rice were well developed. Rice in leaf anatomy had small and fine epidermal cells, chlorophyllous mesophylls, and bulliform cells but had no chlorophyllous vascular bundle sheath cells, while barnyardgrass leaf had large, rough and irregularly arranged epidermal cells, chlorophyllous vascular bundle sheath cells, and non-bulliform cells but had no chlorophyllous mesophylls. Epidermal cells of transplanted rice, however, were well developed, differentiated and sclerified. Cross sections of rice root under dry condition showed cell contents, regularly arranged cells, non-intercellular spaces and non-aerenchyma while under water condition had well-developed intercellular spaces, aerenchyma cells, small and densely arranged epidermis, sclerified exodermis and sclerenchyma cells. But root anatomy of transplanted rice consisted of finely, regularly arranged epidermis, well-developed intercellular spaces and nucleous cells.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.2
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• pp.115-127
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• 1973

Leaf discoloration of IR667 lines (tropical) and leading locals (temperate) in fields was classified according to the probable causes and nutritional disorder due to soil reduction in 1972 was investigated. 1. The causes of leaf discoloration in IR667 were low air temperature, soil reduction, seed born, insect bite, nitrogen depression, overdose pesticide, strong wind, early senescence and unknown one. 2. Leaf discoloration due to soil reduction which has been called Sageumbyeong by famers, was caused by the heavy application of $Ca(OH)_2$, compost and poor drainage followed by Zn and K deficiency and Fe toxicity. 3. About 30 days after transplanting deficiency concentration of K and Zn in leaf blade appears to be less than 2.0% and 20ppm respectively, and greater than 200ppm, 500ppm, and 1.0% respectively for toxicity or excess of Fe, Mn and Ca. and in the shoot 2.4% for K, 30ppm for Zn and 800ppm for Fe. The value of K/Ca should be greaterthan 2.0 for health. 4. When plants were damaged by soil reduction the contents of N, P, Ca, Mg, Fe, Mn, Na in shoot were increased and those of K, Zn, Si were decreased. 5. IR667 lines show in shoot higher content of N, P, Ca, Mg, Si, Na, and lower content K, Zn, Fe, Mn and lower root activity than local leading varietles in either healthy or disieased case, indicating IR667 lines are likely more suseptible to soil reduction damage. 6. Normal soil was less than 6.5 of pH and greater than -50 mv of Eh, but pH of problem soil was ranged from 6.7 to 7.4 and Eh from -100 to -190. 7. The root activity (${\alpha}$-naphthylamine oxidation) decreased at early stage of soil redudtion damage, then increased with severity and at the end it decreased again, but IR667 lines showed always lower root activity than local ones.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.2
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• pp.95-105
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• 1973

Sink (grain)-filling rates of IR667 line (Suwon 213 and 214) and local leading varieties (Jinheung and Paldal) were investigated in relation to sink-source ratio, sink-source distance, forms of photosynthates, and weather factors. The results are as follows. 1. IR667 line have higher maximum filling rate (g. grain/day. ear) and shorter duration to reach maximum filling rate than local varieties. The curve pattern of sink-filling rate was high and steep type in IR667 line and low and broad type in local varieties. 2. Weather factors seem to give little effect on the curve pattern of sink-filling rate. 3. The functional sink-source distance (actual sink-source distance multiplied by the contribution rate of leaf for grain production) was shorter in upper leaves (flag and 2nd leaf) for IR667 line, the upper leaf-dependent type in grain production, and was longer in lower leaves (3rd and 4th) for local varieties, the lower leaf-dependent type. Specially short first internode from top may contribute to the upper leaf depencency of IR667 line. 4. According to free sugar-starch ratio (sugar/starch) in the culm and leaf sheath IR667 line could be classified as the high sugar type and the local varieties as the high starch type. The ratio of transportable form (sugar) to non transportable photosynthate (starch) seems to relate with sink-filling rate. And high sugar type is expected to have higher efficiency for grain production in view of energy balance. 5. A hypothesis that the higher in the uniformity within the series of productive structure the more efficient in grain production is proposed and discussed in relation to productivity. 6. According to the pattern of percent nutrient retention of each leaf blade IR667 showed the central retention type and Jinheung showed the apical retention type and each retention type appears to be a cause of each canopy conservation pattern. 7. From the content and percent distribution of nutrient in various organ IR667 could be classified as the leaf sheath dominant type and Jinheung as the leaf blade dominant type. 8. The fact that the greater the percent translocation of nutrient into grain the greater the percent nutrient retention in leaf blade was held between nitrogen and phosphorus within a variety and between varieties within a nutrient (N, P or K).

• Korean Journal of Organic Agriculture
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• v.7 no.1
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• pp.115-127
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• 1998

Under the visual judgement of consumers, to reduce nitrate intake through vegetables, this experimentation analyzed the content of nitrate, in heading leaf vegetables such as chinese cabbage(Brassica campestris L. ssp. perkinensis (Lour.) Rupr), cabbage(Brassica oleracea L. var. capitata) and lettuce(Lactuca sativa L.) by the leaf number. And the result is summarized as follows In the nitrate content change by the leaf number, the nitrate content is increased as it goes by from inner leaf to outer leaf and the nitrate content in leaf midrib is higher than that in leaf blade. In case of chinese cabbage, the nitrate content in the leaf midrib from the most inner leaf to the most outer leaf changed 40-3,177ppm and in the leaf blade it changed 40-2,887ppm. But the nitrate content in the leaf blade of cabbage from the most inner leaf to the most outer leaf changed 89~2,297ppm and in the leaf blade it changed 25~765ppm. In case of lettuce, the nitrate content change of the leaf midrib by the leaf position was 419~4,349ppm, and in the leaf blade it changed 260~2894ppm. It was conclude that the outer leaf of chinese cabbage, cabbage and lettuce should be removed to keep the lower nitrate intake by population before it is consumed.