• Korean Journal of Microbiology
• /
• v.36 no.4
• /
• pp.292-298
• /
• 2000

For scrutinizing the changes of aggregated bacteria in Lake Paldang, the FISH method was applied by using the rhodamine labeled probes, and total bacteria, chloropyll a concentrations and nutrients concentrations were measured. The aggregates were collected with sediment traps. The T-N, T-P, chlorophyll a concentrations of aggregates were higher 5-15 times, 81-140 and 49-66 times than water samples, respectively. Also, the bacterial numbers of aggregates were 200 times higher than those of water smaples. The ratios of each groups of water sample were 2.1-7.4% for $\alpha$-group, 4.5-8.3% for $\beta$-group, 2.1-7.4% for $\gamma$-group, 2.1-6.1% for Cytophaga-Flavobacterium group and 0.1-2.5% for 'other'group, respectively. While, in aggregates, the ratios of $\alpha$-, $\beta$-, $\gamma$- and Cytophaga-Flavobacterium groups were very small and most abundant group was 'other' bacteria. With these results, the aggregated bacteria in Lake Paldang had a particular group composition of bacteria.

• Korean Journal of Ecology and Environment
• /
• v.41 no.2
• /
• pp.237-246
• /
• 2008

Filtration rates and fecal production of freshwater bivalve, Unio douglasiae on two kinds of hibernal diatom communities were measured simultaneously in a laboratory. One community is the Han River (HAN), which dominated by Asterionella Formosa. Stephanodiscus hantzschii (ca. 98% of total phytoplankton). The other community is the Ilgam Lake (IL), which dominated by Synedra ulna, Scenedesmus sp. Microcystis aeruginosa (ca. 82%). The HAN water has higher concentrations of nutrient (TN and TP) and chlorophyll $\alpha$ (Chl-$\alpha$), lower turbidity and conductivity than the IL water. Water sampling for the feeding experiment was conducted in the same day (Jan 15, 2008) and similar time (AM 10:00 for HAN, AM 11:00 for IL). Mussels with the similar size ($0.0{\pm}0.5\;cm$) were collected from the Gunsan and Okgu district (Jeonbuk), and starved in a laboratory for 2 days before the experiment. The experiment comprised CON (no addition of mussel), LOW (addition of mussel at 0.3 indiv. $L^{-1}$), MID (1.0 indiv. $L^{-1}$) and HIGH (2.0 indiv. $L^{-1}$), respectively. With the increment of mussel density and time, the concentration of Chl-$\alpha$ in two diatom communities were clearly decreased; Chl-$\alpha$ of HAN gradually decreased after 1 hour of mussel treatment, while that of IL decreased as soon as mussel introduction. In 7 hours of treatment, the former was removed finally up to about 90% of control, while the later was remained as about 50%. Under the presence of mussel, total phytoplankton density was shifted as the similar patterns to that of Chl-$\alpha$ (r=0.705, P<0.0001), however, there showed the drastic differences following a species. Based on the concentration of Chl-$\alpha$, filtration rate of U. douglasiae averaged 0.266 $L\;g^{-1}\;h^{-1}$ (0.115 to 0.442) on HAN and $0.577\;L\;g^{-1}\;h^{-1}$ (0.146 to 1.428) on IL water, respectively. There were no differences in feces production among the mussel density in the HAH water (ANOVA, P>0.5), while in IL water, including lots of seston, the HIGH mussel produced the higher fecal materials, over one hundred times of LOW. These results suggest that freshwater bivalve Unio douglasiae have the alternative potential, as a filter-feeder of seston in turbid lake, and a biological controller of diatom bloom in cold stream.

• The Journal of Natural Sciences
• /
• v.16 no.1
• /
• pp.143-154
• /
• 2005

To define the ecological roles of bacterial community in Daechung Reservoir. Vertical variation of total bacterial numbers were estimated at MAN site for one year with DAPI staining. The bacterial numbers were counted, environmental factors were monitored at the surface, -5m, -10m, -15m and -25 m of MAN sited and tried to explain the vertical bacterial numbers with environmental factors. The bacterial number was $1.6-1.7.0 x10^6$ cells/ml at the surface, $2.3-11.0x10^6$ cells/ml at 5 m depth, $1.2-1.4.0x10^6$ cells/ml at 10 m depth, $1.4-15.0x10^6$ cells/ml at 15 m depth and $1.4-1.3.0x10^6$ cells/ml at 25 m depth. The Mean bacterial number at the surface was more that those at any other depth. The explanation of the vertical total bacterial numbers with environmental factors were suggested.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.11 no.4
• /
• pp.199-213
• /
• 2008

Saemangeum salt-water Lake has been created by the completion of the sea-dike in April 2006. To monitor the water qualities of the lake during the sea-dike construction, salinity, SS, nutrients(DIN, DIP, DISi), and chlorophyll-$\alpha$ was analyzed for the surface water from 1999 to 2007. Due to the dike construction, weaker tidal current and lesser resuspension of bottom sediment resulted in the marked decrease of the concentrations of SS in the lake water. Consequently the clearer lake water has provided better condition for primary production with deeper penetration of sunlight into the water column and sufficient nutrient content in the water. Finally the chlorophyll-$\alpha$ content became approximately double in the concentration after the dike construction. Highly stimulated algal production with the marked decrease of the concentrations of SS was decreased the concentration of DIP in the surface water. On the other hand the concentration of DIN and DISi in surface water was increased after dike construction due to the expansion of the freshwater and the supply from bottom layer. As a result, the lake revealed an extremely high NIP ratio and a DIP-limited ecosystem. The lake has been transformed from a typical coastal ecosystem to a brackish one. Since the dike completion, the lake has shown a similar change pattern to the Geum River estuary. Due to the salt-wedge intrusion of seawater, it is highly probable to expect the formation of low-oxygen zone at the bottom layer near the river-mouth area of the lake during the summer. Therefore we need a continuous sentinel monitoring of bottom water qualities in the near future.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.15 no.5
• /
• pp.3279-3285
• /
• 2014

This study aimed to understand seasonal and geographical characteristic of chlorophyll-${\alpha}$ (chl-${\alpha}$), COD (chemical oxygen demand) and Escherichia coli at Gwangyang Bay during the period from February 2010 to November 2012. The bay is divided into three different zones based on the pollutant levels and geographical characteristics. During the study periods, water temperature, salinity, Chl. ${\alpha}$, and chemical oxygen demand (COD) varied in the range of $4.68-28.63^{\circ}C$, 1.94-33.84 psu, 0.31-35.10 ${\mu}gL^{-1}$, and 0.70-13.35 $mgL^{-1}$, respectively. Total chl-a concentration were high at the zone I, which can be characterized as a semi-enclosed eutrophic area, and it were low at the zone III, which is influenced by low nutrients of surface warm water current from offshore of the bay. The high concentration of COD was observed at inner bay during the four seasons and the water quality level was kept to be bad condition during spring season at the zone II, which is influenced by Seomjin River water. The highest colony form of E. coli was recorded to be 3550 $cfuL^{-1}$ during summer at station 1 (zone I), whereas it was relatively kept low level during all seasons at the zone III. As a result, the E. coli was correlated with water temperature (r=0.31 p<0.05) and salinity (r=-0.55 p<0.05), implying that those parameters have play an important crucial role in proliferation of E. coli. Consequently, our results indicated that the E. coli can be significantly promoted within pollutant sources including the high nutrients supplied by rive discharge during spring and summer rainy seasons in semi-enclosed area of Gwangyang Bay.

• Korean Journal of Environmental Biology
• /
• v.19 no.2
• /
• pp.129-135
• /
• 2001

The effects of CellCaSi and bioflocculant on the control of algal bloom were investigated in enclosures in a small eutrophic pond. The bioflocculant produced by a bacterial strain S-2 was finally selected to remove Microcystis aeruginosa which was a dominant species of algal bloom in the pond. Enclosure experiment showed that phosphorus concentration decreased dramatically from $131\mu{g}\ell^{-1}$ (Control) to $1-14\mu{g}\ell^{-1}$ in three CellCaSi-enriched enclosures. Chlorophyll $-\alpha$ concentration also decreased from $215\mu{g}\ell^{-1}$ (Control) to $59\mu{g}\ell^{-1}$ by the addition of CellCaSi $(1g\ell^{-1}$, bioflocculant $(2ml\ell^{-1}$, calcium chloride $(1g\ell^{-1}$ and ferric chloride $(2mg\;Fe\ell^{-1})$ in Enclosure 4. From the results of the mouse acute toxicity test of the S-2 bioflocculant and the goldfish survival test in enclosures, it seems that both the S-2 bioflocculant and the CellCaSi do not show any severe toxicity in water system. Consequently, it was concluded that the bioflocculant and the CellCaSi could be used to control algal bloom in eutrophic waters by removing phosphorus and chlorophyll$-\alpha$.

• Korean Journal of Environmental Biology
• /
• v.26 no.2
• /
• pp.76-86
• /
• 2008

The seasonal variations of primary production were investigated by phyto-PAM chlorophyll fluorometry as well as classical methods (standing crops of phytoplanktons and chlorophyll ${\alpha}$) in the Beopsu Marsh, Haman-gun, Gyeongsangnam-do. The amounts of turbidity, SS, T-N, T-P, BOD, COD, Ca$^{2+}$ and Cl$^-$ were the highest at the station 3, where located in flowout site of wastewater treated by the filtration plants. The water quality was the third level by the standard of BOD and COD. The amount of chlorophyll a (268.8 mg L$^{-1}$) was the highest at the station 2 in April because the cell density (2,677 cells mL$^{-1}$) of Micractinium pusillum increased suddenly from February (180 cells mL$^{-1}$). The patterns of primary production of phytoplankton by phyto-PAM chlorophyll fluorometry were fallen in with those of standing crops and chlorophyll a of phyto-planktons. The primary production was varied according to stations and seasons. The water environments of the Beopsu Marsh as a natural mounument should be under the control of a regular examination in order to preserve the ecosystem.

• Korean Journal of Environment and Ecology
• /
• v.29 no.5
• /
• pp.743-752
• /
• 2015

We studied the spatio-temporal microalgal (phytoplankton and benthic diatoms) and environmental changes of the Hajeonri intertidal zone from February to November 2014. Seawater and phytoplankton analysis were conducted all through the months. The species, composition and abundance of phytoplankton and environmental factors were measured. As a result, diatom was dominant among a total of 113 species identified. On a seasonal basis (Feb. May, Aug., Nov.), we carried out studies on benthic diatoms on the surface of the sediments and their habitats at nine stations on the transect line at Hajeonri at low tide. The grain of the surface sediments was mainly composed of sandy silt and sandy mud. Organic pollution level was low. Benthic microalgal biomass (chlorophyll ${\alpha}$) was high in the spring and summer. A total of 163 benthic diatom species were identified. Navicula sp.1 and Paralia sulcata were dominant over the study period. Five diatom species were observed both in water column and on surface sediment at the same time.

• Korean Journal of Ecology and Environment
• /
• v.41 no.spc
• /
• pp.86-92
• /
• 2008

This study was conducted to determine removal effect on phytoplankton (chlorophyll-${\alpha}$ as whole algae) and cyanobacteria by a fish Pseudorasbora parva and macroinvertebrate Palaemon paucidens in September 2006. Three treatments with 25 (T1), 50 (T2) and 100 (T3) individuals along with control (C1, no input fish), and two treatments with 25 (T4) and 50 (T5) individuals along with control (C1) were made for fish and macroinvertebrate, respectively. The initial concentrations of chlorophyll-${\alpha}$ $(Chl_i)$ in each 10L test tank were set up for the levels of $95{\sim}100{\mu}g\;L^{-1}$ and the daily values were monitored in the test tank during 7 days. In the lab tests, P. parva did not show Chl-${alpha}$ removal effect; the removal rate of Chl-${alpha}$ for P. parva was -58% in T1, -56% in T2, and 61% in T3 during the test period. In contrast, P. paucidens. in the treatments of T4 and T5 removed the phytoplankton effectively and the removal effect were appeared to be 33% and 22%, respectively. Also, P. paucidens showed high feeding efficiency in the removal of cyanobacteria. The levels of cyanobacteria were greatly lowed from 6,048 to 927 cells $mL^{-1}$ in T4 and from 6,539 to 1,053 cells $mL^{-1}$ in T5, resulting in 85% and 84% in the removal effect, respectively. Our results for biomanipulation tests suggest that P. paucidens may be used as a potential candidate organism for algae control in spite of the preliminary results by laboratory tests.

• Korean Journal of Ecology and Environment
• /
• v.41 no.spc
• /
• pp.50-60
• /
• 2008

We performed the experiment to evaluate the effect of different DO concentrations (0.5, 4.5 and 9.0 $mgO_2L^{-1}$) and water depths (20, 50 and 80 cm) on the filtering rate, mortality, and pseudifeces production of Unio douglasiae against the cyanobacterial bloom (mainly Microcystis aeruginosa). A solitary-living bivalve U. douglasiae was collected in the upstream region of the North Han River (Korea). The harvested mussels were carefully transferred to the laboratory artificial management system, which was controlled temperature $(18{\pm}2^{\circ}C)$, flow rate (10L $h^{-1}$), food $(Chlorella^{TM})$, sediment (pebble and clay), light intensity (ca. $20{\mu}mol$ photons), and photocycle (12 L : 12 D). In the field observation, the mussel mortality was significantly correlated with water temperature, pH and DO concentration (P<0.05). The mortality was decreased with water depth; 65, 90, 80% of mortality at 20, 50, 80 cm water-depth, respectively. Filtering rate (FR) showed the highest value at 50 cm water depth, and thereby the concentration of chlorophyll-${\alpha}$ decreased continuously by 94% of the control at the end of the experiment. In contrast, FR decreased by 34% of the initial concentration at 20 cm water depth. Over the given water-depth range, the mussel FR ranged from $0.15{\sim}0.20L\;gAFDW^{-1}hr^{-1}$ during the 18hrs of experiment, and thereafter, they appeared to be approximately 0.11, 0.26 and 0.30 L $gAFDW^{-1}hr^{-1}$ at 20, 50 and 80cm water depth, respectively. FR was highest with the value of 0.46L $gAFDW^{-1}hr^{-1}\;at\;0.5mgO_2 L^{-1}$ at the early stage of the experiment, while it increased with DO concentration. Maximum pseudofaeces production was 11.2 mg $gAFDW^{-1}hr^{-1}\;at\;9.0mgO_2L^{-1}$. Our results conclude that U. douglasiae has a potential to enhance water quality in eutrophic lake by removing dominant cyanobacteria, but their effects vary with environmental parameters and the water depth at which they are located.