• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.3
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• pp.159-163
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• 2002

This study was analyzed to photosynthetic pigment concentrations and changes of SOD activities on seven species of liana of A. heterophylla, P. scandens, V. thunbergii, P. tricuspidata, C. trilobus, L. japonica and T. kirilowii, and two species of E. arvense and A. princeps of non climbing plants. Concentrations of chlorophyll a and b, total chlorophylls and total carotenoids of P. tricuspidata in 100 ppb ozone site were the most increased. It was the most increased to P. scandens in ratio of chlorophylls and carotenoids, and E. arvense in ratio of chlorophyll a and b. There was difference to ratio of chlorophyll a and b of liana and non liana. At ratio of chlorophyll a and b of 100 ppb ozone site and the control it was more sensitive to chlorophyll a than chlorophyll b, and P. tricuspidata was the most sensitive at comparing with species, and it was more sensitive to liana than non liana. In SOD activities A. princeps was the most increased to 3535.7 unit/g, and P. scandens was the fewest increased to 109.3 unit/g, and A. heterophylla was only decreased to 131.7 unit/g in comparing to 100 ppb ozone sites and the control.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.3
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• pp.263-267
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• 2002

This experiment was conducted to find out the varietal differences in the relationships of chlorophyll and nitrogen concentration with leaf greenness value (SPAD value). We used two rice varieties with different leaf color type: Hwaseongbyeo has pale green leaves, and Gwanganbyeo has dark green leaves. SPAD value was correlated with chlorophyll a concentration significantly in both varieties, but not with chlorophyll b in Hwaseongbyeo. The correlation coefficients between SPAD value and nitrogen concentration of rice flag leaves were highly significant. However, the regression analysis between SPAD value and nitrogen concentration of rice flag leaves showed different relation among varieties. The slope of the regression line in Gwanganbyeo was steeper than that in Hwaseongbyeo. In conclusion, SPAD value can be applied to estimate the chlorophyll a concentration without consideration of varietal differences. Chlorophyll b and nitrogen concentration in the leaf were regressed to the SPAD values differently with varieties. This implies that the SPAD value can not be used directly to estimate chlorophyll b and nitrogen concentration without consideration of varietal differences.

• Journal of Life Science
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• v.29 no.5
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• pp.509-513
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• 2019

The Beer-Lambert law states that absorbance is proportional to the concentration of a solute in a solution at a given wavelength. This linearity works for an ideal or a 'sufficiently diluted' solution, so this linearity is often used as a criterion for the fidelity of the absorbance value measured. In this study, we used a chlorophyll (Chl) solution, isolated from rice leaves with 80% acetone to test the use of the Chl a/b ratio as an additional criterion for checking the fidelity of measured values using four different absorption spectrophotometers: Cary4E, UV-1650PC, Versamax (a microplate reader), and NanoDrop 1,000(which can handle a $4{\mu}l$ aliquot). We used Chl solutions of varying concentrations from $0.2{\mu}g/ml$ to $200{\mu}g/ml$ to measure absorbance values at 645 nm and 663 nm and checked the linearity first. The results indicated that the range of Chl concentrations that we can rely on based on the linearity was similar to the range in which the calculated Chl concentrations based on the measured absorbance values agreed with the known concentrations. However, some border cases or cases with very low Chl concentrations inside the fidelity range of Chl concentrations did not agree with the criterion that the Chl a/b ratio should not change after dilution of the Chl in the solution. These results suggest that the Chl a/b ratio is a better criterion for the reliability of the absorbance values measured for the determination of chlorophyll concentration than the criterion based on the linearity suggested by the Beer-Lambert law.

• Journal of Life Science
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• v.32 no.10
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• pp.743-748
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• 2022

The garlic chive (Allium tuberosum Rotter) is a prominent herb species in Asia and other nations of the world. Garlic chives is a favorite vegetable and used to garnish noodles in Korea. The effects of various doses of N, P, and K fertilizers and drought stress on the chlorophyll content in the leaves of garlic chives were investigated. The evaluations showed that chlorophyll a content was 0.386 at 10 mg/l N fertilizer and 0.584 at 50 mg/l N fertilizer. The treatment group showed a significant difference with regard to the contents of chlorophylls a and b and total chlorophyll at the 5% level (p<0.05). Pearson's correlation coefficient (Pearson's r) for chlorophylls a and b and total chlorophyll were 0.940, 0.966, and 0.971, respectively. The highest content of chlorophylls a and b and total chlorophyll in the leaves was recorded at 40 mg/l P fertilizer, while the values corresponding to 50 mg/l P fertilizer were lower than those for 40 mg/l P fertilizer. The content of total chlorophyll evaluated at 10 mg/l K fertilizer was 0.312 and that at 50 mg/l was 0.589. The simple linear regression showed the relationship between chlorophyll efficiency aand moisture. The slope factors of the dark-level fluorescence yield (Fo), the maximum fluorescence yield (Fm), the quenched state (Fv), and the maximal PSII quantum yield (Fv/Fm) for chlorophyll-efficient indicators were -0.931, 0.972, 972, and 0.950, respectively. NPK fertilizers and drought stress affected the chlorophyll content and efficiency of A. tuberosum.

• Journal of Environmental Science International
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• v.5 no.5
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• pp.643-656
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• 1996

Various light intensity and light quality were treated to oat seedlings to investigate the effect of light on the chlorophyll accumulation and the formation of chlorophyll-protein complexes. The Increase of total chlorophyll accumulation and Chl (chlorophyll) a/b ratio was promoted under H (high intensity) white light during oat chloroplast development when compared to 1 (low intensity) white light. Also H white light was more effective in the formation of chlorophyll-protein complexes associated with PSI CCI and CCII than L white light. The seedlings grown in various right quality caused little changes in total chiorophyl and ChI-a/b ratio when compared to those grown in L white light. The assembly of LHCII trimer was more affected by L white light treatment in the formation of-chlorophyll-protein complexes than red light treatment. The effect of blue light on the relative composition of chlorophyll-protein complexes was similar to that of L white light. Particualrly, blue light was more effective in the synthesis of LHCII monomer than the other light quality at the early stage of greening. When compared to red light, blue light was more effective the increase of chlorophyll accumulation and Chl a/b ratio than 1ight Quality, and light Quality may be in important factor for the regulation of the organization in the chlorophyll-protein complexes curing greening.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.1
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• pp.163-169
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• 1996

인위돌연변이 유기에 의해 벼로부터 새로운 유전형질체를 개발하기 위하여 낙동벼(모품종)에서 고정된 엽록소 결핍계통(ch mutant)을 선발하였다. 이 계통은 생육 초기부터 엽록소결핍으로 정상엽에 비하여 노란색을 나타내기 때문에 엽록소의 광합성 관여 유전자탐색에 이용가능하다. ch mutant는 전 생육기간 동안 모품종의 51~87% 정도의 엽록소 함량을 보였으나 엽록소 a/b 함량비는 차이가 없었다. ch mutant의 총 엽록소 함량은 모품종의 70.2%였으면 anthocyanin 함량과 flavonol 함량은 각각 285%와 142% 로 높은 함량비를 나타냈다. ch mutant의 카로티노이드 함량은 모품종의 71.1%였고, 크산토필 함량은 56.6%였다. 특히 카로티노이드 성분중 lutein과 neoaxanthin 함량은 각각 모품종의 32%와 34.4%로 매우 낮았으며, $\beta$-카로틴은 차이가 없으나 antheraxsnthin 함량은 106.9%오히려 증가하였다. 개엽상태에서나 군락 상태에서 ch mutant의 반사율과 투과율은 모두 모품종에 비하여 높아 광흡수량은 저하되었다. ch mutant는 모품종에 비하여 출수기가 5일 늦고 간자, 수장, 주당수수, 수당 입수, 임실율, 천립 중 수량에 감소하였다. ch mu-tant와 모푸종은 esterase, phosphogluose isomerase, malic enzyme, hexokinase 동위 효소의 밴드 패턴에서 유의할 만한 차이를 나타내지 않았으나 엽록체 단백질의 경우 주요 밴드인 60KD의 분자량에서 차이를 보였다.

• Journal of Bio-Environment Control
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• v.19 no.1
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• pp.36-48
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• 2010

This present study was conducted to investigate photosynthetic characteristics, chlorophyll fluorescence, chlorophyll contents of Betula platyphylla var. japonica, Prunus leveilleana, Magnolia sieboldii, growing under four different light intensity regimes (full sun, and 64~73%, 35~42%, 9~16% of full sun). As result, Betula platyphylla var. japonica showed outstanding photosynthetic capacity and apparent quantum yield in full sun and showed low shade tolerance. Prunus leveilleana showed good photosynthetic capacity and apparent quantum yield in 64~73% or 35~42% of full sun and showed common shade tolerance. However, Magnolia sieboldii showed good photosynthetic capacity and apparent quantum yield in 35~42% of full sun, while the lowest in full sun. Magnolia sieboldii showed the highest shade tolerance compared to the other species. As the shading level increased, the total chlorophyll contents of all species increased with significant difference.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.4
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• pp.327-338
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• 2015

Because of impact on the underwater light field, CDOM can influence the accuracy of global satellite-based measurement of ocean chlorophyll and primary productivity. So we investigated the distribution and seasonal variation of CDOM in the East Sea during summer 2009 and 2011. Among them we report two distinctively different summer cases between 2009 and 2011 year, in which showed the different main sources for CDOM. Regulating factors and sources of CDOM in the East Sea were examined. Comparison between in situ and satellite derived Chl a and CDOM were made to find an influence of CDOM on measurement of satellite derived Chl a. Similar pattern and matching of MODIS Chl a with in situ Chl a 2009 was comparable, but significant discrepancy between MODIS Chl a and in situ Chl a was found, when CDOM was high in summer of 2011. GOCI data showed better matching with in situ data for both Chl a and CDOM, compared to MODIS data in summer of 2011. The presence of high CDOM at the surface layer supplied by vertical mixing seems to affect on the overestimation of Chl a by satellite data.

• Journal of Life Science
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• v.6 no.4
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• pp.299-303
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• 1996

The effects of ozone (O$_{3}$) exposures on chlorophyll content of trembling aspen(Populus tremuliodes) rooted cuttings were studied. Ozone was treated with 100 ppb fumigation for 6 hours per day, for three consecutive days per week in open-top chamber. The ozone exposures caused severe foliage damage expressed in necrotic patches and extensive chlorosis. Degradation of chlorophyll was observed mostly in mature leaves. The chlorophyll response to ozone exposure maintained a similar pattern during all experimental seasons and plant materials, Significant genetic variation in O$_{3}$ responses was revealed by differences in sensitivies among clones.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.43-48
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• 2003

The effect of rare earth(RE) at three difference levels of nitrogen on the growth and nitrate content of chicory (Cichorium intybus L.) were studied in terms of leaf size, fresh and dry weight increase, and chlorophyll and nitrate content grown in plastic pots within glasshouse. Leaf size was enlarged as the level of nitrogen applied was high, and leaf within, length, and thickness of leaf were increased by RE treatment showing significant difference at high nitrogen(N+1) plot. As the level of nitrogen applied was high, as fresh and dry weight per plant was increased significantly(p<0.05), and sprinkling cabbage with RE increased fresh and dry matter yield to 2∼12, 4∼6.2% more, respectively. Dry matter content of Chinese cabbage was increased by RE sprinkling. At all levels of nitrogen fertilized chlorophyll contents were increased by RE treatment. As the level of nitrogen was high, as the content of chlorophyll was highly increased by RE. Nitrate was accumulated more at high level of nitrogen application, but nitrate was decreased by RE application, 40% decrease at very high nitrogen plot(N+2). RE stimulated the growth of chicory with high chlorophyll content, and showed the possibility producing high quality agricultural products low in nitrate content.