• Title/Summary/Keyword: 연잎 추출물

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Effect of Sasa Borealis and White Lotus Roots and Leaves on Insulin Action and Secretion In Vitro (In vitro에서 조릿대, 연근과 연잎이 인슐린 작용 및 분비에 미치는 영향)

  • Ko, Byoung-Seob;Jun, Dong-Wha;Jang, Jin-Sun;Kim, Ju-Ho;Park, Sun-Min
    • Korean Journal of Food Science and Technology
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    • v.38 no.1
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    • pp.114-120
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    • 2006
  • Anti-diabetic effects of extracts and fractions of Sasa borealis (SB), white lotus roots (LR) and leaves (LL), and their mixture were determined in 3T3-L1 adipocytes and Min6 cells by investigating insulin-sensitizing activity and glucose-stimulated insulin secretion, respectively. SB, LR, LL, and mixture of SB, LR, and LL (3 : 2 : 3) were extracted using 70% ethanol, and m mixture extract was fractionated by XAD-4 column chromatography with serial mixture solvents of methanol and water. Fractional extractions were utilized for anti-diabetic effect assay. SB and LR extracts increased insulin-stimulated glucose uptake, but not as much as mixture of SB, LR, and LL. Significant insulin-sensitizing activities of 20 and 80% methanol fractions of SB, LR, and LL mixture extract were observed in 3T3-L1 adipocytes, giving 0.5 or $5\;{\mu}g/mL$ each fraction with 0.2 nM insulin to attain glucose uptake level similar to that attained by 10 nM insulin alone. Similar to pioglitazone, peroxisome proliferators-activated $receptor-{\gamma}\;(PPAR-{\gamma})$ agonist, 20 and 80% methanol fractions increased adipocytes by stimulating differentiation from fibroblasts and triglyceride synthesis. LL extract and 20, 60, and 80% methanol fractions of the mixture suppressed ${\alpha}-amylase$ activity, but did not modulate insulin secretion capacity of Min6 cells in both low and high glucose media. These data suggest 20 and 80% methanol tractions contain potential insulin sensitizers with functions similar to that of $PPAR-{\gamma}$ agonist. Crude extract of SB, LR, and LL mixture possibly improves glucose utilization by enhancing insulin-stimulated glucose uptake and inhibiting carbohydrate digestion without affecting insulin secretion in vivo.

Anti-oxidant and Whitening Effects of Cell Lytic Enzyme-treated Lotus Leaf Extract (세포벽 분해효소 처리에 의한 연잎 추출물의 항산화 및 tyrosinase 저해 활성)

  • Choi, Sun-Ju;Kim, So-Young;Lee, Sung-Chul;Lee, Jin-Man;Lee, In-Suk;Jung, Moon-Yung;Yang, Sam-Man;Chae, Hee-Jeong
    • KSBB Journal
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    • v.24 no.6
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    • pp.579-583
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    • 2009
  • The effects of cell lytic enzyme treatment on total phenolic content, antioxidant and antityrosinase activities of lotus leaf were investigated. The dried lotus leaves were hydroyzed by cell lytic enzymes such as Promozyme, Ceremix, Pectinex, Ultraflo, Celluclast, Pentopan, Tunicase, Viscozyme at their optimum pHs (pH 5-8) at $50^{\circ}C$ for 4 hrs. Depending on the enzymes used, total phenolic compounds content was measured as $1,079-1,476{\mu}g$/mL, and antioxidant activities and whitening activities were increased by 5~10% and 20%, respectively Among the tested hydrolytic enzymes, Promozyme (pullulanase) was selected as the most suitable enzyme for the extraction of total polyphenol from lotus leaf. The optimal dosage of Promozyme were found to be 1-2% (w/w). By Promozyme treatment, total phenolic compounds content of the lotus extract significantly increased compared to the extraction without enzyme treatment.

Stability Evaluation of the Cosmetics Containing Lotus Leaf Extract (연잎 추출물을 함유한 화장품의 안정성 평가)

  • Choi, Sun-Ju;Kim, So-Young;Jeong, Yoon-Joo;Ku, Chang-Sub;Ha, Buung-Jhip;Chae, Hee-Jeong
    • KSBB Journal
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    • v.26 no.1
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    • pp.83-86
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    • 2011
  • The stability of cream containing lotus leaf extract (LLE) was evaluated. Stability pH and viscosity were measured at 3 different temperatures ($25^{\circ}C$, $45^{\circ}C$ and $65^{\circ}C$), and at room temperature under the sun light at 2 day intervals during 12 days. The cleansing cream and foam cleansing containing the extract did not show a significant pH change at the different temperatures. However, the pH of the cleansing cream and foam cleansing containing LLE slightly decreased at $25^{\circ}C$. They showed a high stability at temperature conditions ($45^{\circ}C$ and $65^{\circ}C$) and under the sun light condition. The cleansing cream and foam cleansing containing LLE did not show a significant viscosity change at $25^{\circ}C$, but had a tendency of decrease at $45^{\circ}C$ and $65^{\circ}C$. There was no significant offflavor and discoloration as well as physical changes such as creaming and cohesion at $25-65^{\circ}C$ and under the sun light condition. And this LLE could be used as a stable functional cosmetic material.

Effects of ethanol extract of Nelumbo nucifera leaves on Anti-oxidation and Type I Procollagen Expression in CCD-986sk Cells (연잎 에탄올 추출물이 피부 주름에 미치는 영향)

  • Yang, Woong-Mo;Kim, Hyang-Mi;Chang, Mun-Seog;Park, Wan-Su;Kim, Won-Nam;Kim, San-Woong;Choi, Dong-Gi;Lee, Hak-Chul;Kim, Yun-Kyung;Park, Seong-Kyu
    • Herbal Formula Science
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    • v.14 no.2
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    • pp.67-75
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    • 2006
  • The natural plants were screened for new cosmetic materials. Many parts of Nelumbo nucifera has been investigated for anti-wrinkling and anti-furrowing effects. The leaves. flowers and stamens of Nelumbo nucifera were extracted with ethanol or distilled water. The effects of cell viability and $H_2O_2-induced$ cytotoxicity were studied on CCD-986sk human fibroblast cell line by MTT assay. We measured various effects related to skin such as the synthesis of MMP-1 and type I procollagen. The ethanol extract of Nelumbo nucifera leaves (ENL) at concentrations of 30 ${\mu}g/ml$ showed maximum cell viability on CCD-986sk cells by 129.70%. and $H_2O_2-induced$ cytotoxicity was blocked by ENL (5. 10. 20. 30. and 40 ${\mu}g/ml$) concentration-dependently. The synthesis of MMP-1 and type I procollagen were positive in ENL. These results suggest that ENL has anti-oxidant and anti-furrowing effects.

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Antioxidative Activity and Nitrite Scavenging Ability of Ethanol Extract from Nelumbo nucifera Leaves (연잎 에탄올 추출물의 항산화 효과 및 아질산염 소거능)

  • Lim, Jin-A;Lee, Eun-Sook;Baek, Seung-Hwa
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.3
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    • pp.654-659
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    • 2008
  • To confirm possibility of Nelumbo nucifera leaves as biofunctional material, we investigated the antioxidant activity and nitrite scavenging ability of ethanol extract from Nelumbo nucifera leaves. Nelumbo nucifera leaves were extracted with ethanol and concentrated under vacuum using rotary evaporator. Then, antioxidative activity and nitrite scavenging ability of the extract were examined in vitro. Electron -donating ability of the extract at RC50 was 90.19 ${\mu}g/mL$. After addition of 0.96 mg/mL, autooxidation of pyrogallol decreased to 66.19% by superoxide dismutase-like activity. In antioxidative activity of the extract against linoleic acid during incubation times of 24, 48, and 96 hours at $40^{\circ}C$, lipid peroxidation values significantly decreased to 72.53%, 82.00%, 84.69% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and the value revealed to be $282.84\;{\pm}\;9.03$ GAE ${\mu}g/mg$ of the extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, exhibited to 45.55% by addition of 0.2 mg/mL. These results suggest that ethanol extract from N. nucifera leaves can be used as bioactive and functional material.

In Vitro Antioxidant Activities and Antimicrobial Activity of Lotus (Leaf, Stem, and Seed Pod) Extracts (연잎, 연 줄기 및 연자방 추출물의 In Vitro 항산화 활성과 항균 활성에 관한 연구)

  • Lee, Ki-Won;Kim, Yong-Hwan;Shin, Kyung-Ok
    • The Korean Journal of Food And Nutrition
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    • v.30 no.4
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    • pp.771-779
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    • 2017
  • The purpose of this study was to investigate and analyze the total phenolic content (TPC), antioxidant activities (FRAP, ABTS, and DPPH), and antibacterial properties of lotus (Nelumbo nucifera) extracts. Lotus leaves, stems, and seed pods were extracted with deionized water at $95^{\circ}C$, and with 70.5% ethanol at $85^{\circ}C$. The TPC ranged from 8.12 to 215.12 GAE mg/g. The ethanol extract of the seed pod had the highest TPC, and the TPC of the corresponding deionized water extract was 161.45 mg/g. FRAP values ranged from 104.03 to $3,546.39TEAC\;{\mu}mol/g$, ABTS radical cation scavenging activities ranged from 105.11 to $3,956.94TEAC\;{\mu}mol/g$, and DPPH radical scavenging activities ranged from 37.29 to $2,549.46TEAC\;{\mu}mol/g$. $EC_{50}$ values ranged from 0.26 to 9.63 mg/mL, and 0.31 to 21.21 mg/mL for ABTS and DPPH, respectively. The ethanol and deionized water extracts of the seed pod showed higher TPC and stronger antioxidant properties (FRAP, ABTS, and DPPH) than those of characteristic of the leaf extracts. The ethanol and deionized water extracts of the seed pod showed antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa with inhibition zones of 9.0 to 14.0 mm, and the ethanol extract of the leaf showed antimicrobial activity against B. subtilis and S. aureus with inhibition zones of 9.0 and 10.0 mm, respectively. Thus, the lotus seed pod could be used to produce novel teas, and could be a potential source of therapeutic ingredients for food and medicine.

Evaluation of Caffeine Contents in Major Flower and Leaf Teas (주요 꽃차 및 잎차의 카페인 함량 평가)

  • Kanphassorn Wimonmuang;Young-Sang Lee;Seung-Young OH;Suk-Keun Park
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.104-104
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    • 2020
  • 꼭두서니과 커피속 식물의주요 2차대사산물인 카페인은 커피나무, 카카오, 차나무 등에서 관찰되는 알칼로이드 화합물로 다른 식물의 발아 억제, 살충 기능이 알려져 있다. 인체에 있어 카페인은 각성효과가 있어 수면 장애나 불안을 유발할 수 있으며 섭취를 중단 시 졸음, 두통 등 금단 증상을 나타나기도 한다. 최근 다양한 식물 종과 부위를 이용한 차 문화가 확산되고 있는 바, 본 연구는 시중에서 판매되고 있는 커피 2종류과 녹차 2종류를 비롯하여 꽃차 37종류, 잎차 15종류, 씨앗차 2종류, 열매차 1종류 및 뿌리차 2종류를 대상으로 메탄올 추출 후 gas chromatography-mass spectrometry를 이용하여 카페인 함량에 대한 정성, 정량 분석을 수행하였다. 대조로 사용된 시판 커피 2개사 제품은 각각 1.18 mg/g, 35.94 mg/g의 caffeine 함량을 나타냈으며 시판 녹차 티백 제품 2종은 1.3 mg/g, 3.42 mg/g의 함량을 나타내었다. 조사된 37종의 꽃차 중에는 차나무 꽃차에서만 1.50 mg/g 수준의 caffeine이 검출되었을 뿐 국내 소비가 확대되고 있는 구절초, 국화, 금계국, 금목서, 금어초, 금잔화, 노랑코스모스, 당아욱, 도라지, 동백나무, 뚱딴지, 라벤다, 마리골드(노랑), 마리골드(주황), 매화, 맨드라미, 무궁화, 백목련, 벚나무, 복사나무, 비단향꽃무, 생강나무, 수레국화, 아까시나무, 연꽃, 유채, 작약, 장미, 진달래, 천일홍, 캐모마일, 패랭이꽃, 팬지, 해당화, 홍화, 히비스커스 등 36종에서는 카페인이 검출되지 않았다. 잎차의 경우 레몬그라스, 레몬밤, 로즈마리, 뽕나무, 세인트존스워트, 스테비아, 쑥, 애플민트, 연잎, 적양배추, 조릿대, 차수국, 티트리, 파인애플민트, 페퍼민트 등 평가된 15종류 모두에서 카페인이 검출되지 않았으며, 씨앗차인 펜넬 및 작두콩, 열매차인 진피차, 뿌리차인 자색당근과 비트차에서 역시 카페인이 검출되지 않았다. 이상의 결과에 기초할 때, 시판되는 다양한 식물 종과 부위를 이용한 차의 경우 대부분 caffeine을 함유하지 않음을 알 수 있었다.

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Factors Affecting Reactivity of Various Phenolic Compounds with the Folin-Ciocalteu Reagent (다양한 페놀성 물질과 Folin-Ciocalteu 시약의 반응성에 미치는 영향 요인 평가)

  • Hong, Jung-Il;Kim, Hyun-Jung;Kim, Ji-Yun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.2
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    • pp.205-213
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    • 2011
  • The Folin-Ciocalteu (F-C) reagent has been extensively used for quantifying total phenolic contents in many different types of food materials. Since several different procedures of the assay methods using the F-C reagent have been applied, we investigated changes in reactivity of various phenolic compounds with the F-C reagent under three different assay conditions and factors affecting reactivity. Among 10 standard compounds tested, compounds with high hydroxyl density (number of -OH/molecular weight) showed a largely different response according to addition sequence of the F-C reagent or $Na_2CO_3$. Preincubation in $Na_2CO_3$ significantly reduced the reactivity of the phenolic compounds bearing galloyl moiety (e.g. gallic acid, tannic acid, and epigallocatechin-3-gallate) with the F-C reagent, while monophenol compounds including ferulic acid and sinapinic acid were more stable as compared to diphenols. There was little change in response to the F-C reagent of all phenolic compounds incubated in acidic pH; their reactivity except ferulic acid was reduced significantly when incubated in neutral or alkaline pH. Changes in reactivity of gallic acid incubated in $Na_2CO_3$ or neutral/alkaline pH conditions were the most prominent. $H_2O_2$ generated from phenolic compounds did not affect the reaction with the F-C reagents. The present results suggest that reactivity of different phenolic compounds with F-C reagent was affected considerably by different procedures of the assay, and the total phenolic contents could be fluctuated according to standard compounds and assay scheme.

A Study on the adequate Aggregate Selection of the Exposed Aggregate PCC Pavements (골재노출 콘크리트포장의 적정 골재 선정에 대한 연구)

  • Kim, Young-Kyu;Chae, Sung-Wook;Lee, Seung-Woo;Yoo, Tae-Seok
    • International Journal of Highway Engineering
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    • v.9 no.4
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    • pp.117-127
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    • 2007
  • The exposed aggregate PCC(EAP) pavements have been successfully used in Europe and Japan as low-noise pavements. Coarse aggregate are exposed on the pavement surface texture of EAP by removing mortar of surface. The pavement surface texture should maintain not only low-noise characteristic but also adequate skid resistance level during the performance period. Skid resistance decreased with wearing and polishing of tire and pavement surface due to the repetition of tire-pavement contact. Since the tires mainly contact the exposed coarse aggregate, the shape and rock type of coarse aggregate significantly influence wearing and polishing of EAP pavements. The test for resistance to abrasion coarse aggregate by use of the Los Angeles machine(KS F 2508) and the method of test for resistance to abrasion coarse aggregate by use of the Accelerated Polishing Machine(ASTM D 3319-90) are generally used to evaluate polishing characteristics of aggregate. In this study, polishing of coarse aggregate of different five rock types were evaluated by KS F 2508(LA abrasion test) and ASTM D 3319-90(PSV method). The results of LA abrasion test and PSV method were contrary to each other. Since LA abrasion test is estimated the quantity of abrasion by the impact of aggregate, it may not be adequate to evaluate the polishing of aggregate by the repetition of tire. In the case of PSV method, the resistance of polishing is estimated the skid resistance variation of polished aggregate after repetition of tire. The PSV method is adequate for the evaluation on polishing of coarse aggregate. From the test results of PSV method, it was founded that rock type, specific gravity, coarse aggregate angularity, flat or elongated particles in coarse aggregate are significant to the resistance characteristic of coarse aggregate.

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Inhibitory Effects of Nelumbo nucifera on Tyrosinase Activity and Melanogenesis in Clone M-3 Melanocyte Cells (연잎, 연꽃 및 연꽃 수술 추출물의 Tyrosinase 활성억제 및 Melanin 생성억제에 의한 미백 효과)

  • Park, Seong-Kyu;Chang, Mun-Seog;Kim, Hyang-Mi;Yang, Woong-Mo;Kim, Do-Rim;Park, Eun-Hwa;Ko, Eun-Bit;Choi, Moon-Jung;Kim, Hyu-Young;Oh, Ji-Hoon;Shim, Kyung-Jun;Yoon, Ji-Won
    • The Korea Journal of Herbology
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    • v.22 no.4
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    • pp.87-94
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    • 2007
  • Objectives : We examined three parts of Nelumbo nucifera that might be useful for skin-whitening effect. Each parts of leaves, flowers and stamen were extracted with water or 70% ethanol. Methods : This study investigated inhibitory effects of each extracts on tyrosinase activity. Extracts were tested for cytotoxicity on clone M-3 melanocyte cells, melanin inhibitory activities were further assessed. Results : The water extract of Nelumbo nucifera stamen. ethanol and water extracts of flowers exhibited inhibitory effects on tyrosinase (IC50 values 726.16, 1063, and 1732.36 ug/mL, respectively). The water extract of Nelumbo nucifera stamen, ethanol extract of flowers, both ethanol and water extracts of leaves showed relatively lower cytotoxicity, with cell viability above 80% with a concentration of 20 ${\mu}g/mL$. In addition, The water extract of Nelumbo nucifera stamen inhibited the melanin production in clone M-3 melanocyte cells. Conclusions : Among each parts of Nelumbo nucifera. the water extract of stamen was the strongest candidates for skin-whitening cosmetic application.

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