• Proceedings of the Speleological Society Conference
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• 1994.05a
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• pp.106-110
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• 1994

$\bigcirc$ 동굴생물은 식물과 동물, 미생물로 크게 구분되지만 태양광선이 완전히 차단된 동굴내 환경에서는 동굴 동물들이 주종을 이루고 있다. $\bigcirc$ 동굴내의 동물에 관한 연구는 1966년 고씨동굴, 용담굴을 시발로 고수굴, 천동굴, 노동굴, 백룡굴, 대이굴, 환선굴 등 강원도내에 분포하는 동굴 중 약 30%정도는 동물을 포함한 생물상이 조사되었다. $\bigcirc$ 강원도의 동굴동물로서 확인된 것온 9종 30목141종이며 강별로는 곤충강이 9목 50종 주형강이 4목 49종 갑각강이 6목 18종의 순으로 우세하고 목별로는 거미목이 15과 39종, 톡토기목이 7과 20종, 딱정벌레목이 8과 15종으로 우세한 편이다 (남, 1987). $\bigcirc$ 동굴생물에 관한 연구는 육상생태계와 격리된 환경에서 생리, 생태적 적응을 통한 종의 분화나 유전, 진화문제를 추구하며 지하생태계와 육상생태계의 생물적인 유연관계 규명에 중요한 분야로 인정되며 아울러 독특한 특성을 지닌 동굴생물의 보존을 위한 방안과 노력이 일부동굴의 개방 및 개발에 앞서 더욱 구체적으로 모색되고 증대되어야 한다. $\bigcirc$ 동굴환경의 주요 특성은 첫째 햇빛이 차단되어 암흑상태이며, 둘째 내부습도가 높고 기온이나 수온의 연중변화가 심하지 않고, 셋째 먹이연쇄에 필수적인 영양공급원이 제한되어 있다. 따라서 광합성작용으로 성장하는 녹색식물은 태양광선이 유입되는 곳이나 인공조명시설 지역을 제외한 곳에서는 서식이 불가능하고 대형동물이나 초식동물은 생존이 어렵다. $\bigcirc$ 동굴내에는 환경에 적응하는 종들만이 서식할 수 있고 이들을 생태적 특성에 따라 세가지로 구분 할 수 있다. 환경적응 요인으로는 광도, 습도, 온도 영양공급원과 섭식장소, 수중생물의 경우는 특히 수온, 수량 영양원등이다.(중략)다. 본 연구의 접근방법으로는 ASRS의 개념적인 Reference Model을 수립하고 이 Reference Model에 대한 Formal Model로 DEVS(Discrete Event System Specification)을 이용하여 시스템을 Modeling하였다. 이의 Computer Simulation을 위하여 DEVS형식론 환경에서의 Simulation Language인 DEVSim ++ⓒ를 이용하여 시스템을 구현하였다.. 실형 결과로는 먼저 선형 상미분방정식의 예로 mass-damper-spring system, 비선형 상미분방정식의 예로는 van der Pol 방정식, 연립 상미분방정식의 예로는 mixing tank problem 등을 보였으며, 그의 공학에서 일어나는 여러 가지 문제들도 다루었다.화물에 대한 방어력이 증가되어 나타난 결과로 여겨지며, 또한 혈청중의 ALT, ALP 및 LDH활성을 유의성있게 감소시키므로서 감잎 phenolic compounds가 에탄올에 의한 간세포 손상에 대한 해독 및 보호작용이 있는 것으로 사료된다.반적으로 홍삼 제조시 내공의 발생은 제조공정에서 나타나는 경우가 많으며, 내백의 경우는 홍삼으로 가공되면서 발생하는 경우가 있고, 인삼이 성장될 때 부분적인 영양상태의 불충분이나 기후 등에 따른 영향을 받을 수 있기 때문에 앞으로 이에 대한 많은 연구가 이루어져야할 것으로 판단된다.태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements)로 가정한다. 즉, [+wh] 의미의 겹의문사는 동일한 구성요 소를 지닌 병렬적 합성어([$[W1]_{XO-}$ $[W1]_{XO}$ ]$_{XO}$)로 그리고 [-wh] 의미의 겹의문사는 중복된 발은을 지닌 한 단어로 ([W]$_{XO}$

• The Korean Journal of Malacology
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• v.25 no.3
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• pp.213-222
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• 2009

In order to investigate contamination of heavy metal in seawater and cultured oyster, samples were collected November 2003 to July 2004 from 12 sites (13 sites for seawater) along the coast of Tongyeong, Korea. The mean concentrations of metal in oyster tissues were as follows: 0.09 (0.01-0.3) ${\mu}g/l$ for Cd, 0.47 (0.01-1.4) ${\mu}g/l$ for Cr, 0.59 (0.2-2.3) ${\mu}g/l$ for Ni, 1.02 (0.1-4.2) ${\mu}g/l$ for Pb and 0.48 (0.01-3.9) ${\mu}g/l$ for Hg in the seawater, whereas 2.45 (0-5.47) mg/kgDW for Cd, 3.63 (0.10-12.91) mg/kgDW for Cr, 3.2 (0.01-15.73) mg/kgDW for Ni, 3.51 (0.01-6.47) mg/kgDW for Pb and 0.39 (0.004-0.74) mg/kgDW for Hg, respectively. Most metal concentration values were below the permissible range for the related regulations. Mean bioconcentration factors (BCF) for each metal were as follows: 38,964 (1,771-207, 171) for Cd, 9,583 (1,231-80, 162) for Cr, 191 (3-20, 980) for Ni, 1,416 (245-5, 207) for Pb and 180 (5-716) for Hg, respectively. The BCF values from this study corresponded to the transitional phase from the pristine to the contaminated waters. Notably, Cd showed the highest BCF, which suggest that the Pacific oyster could be utilized as a useful biomarker for Cd contamination in sea water. The multidimensional scaling analysis suggested that the metal contaminants are mainly originated from combustion of fossil fuel and accumulated to oyster through food web.

• Korean Journal of Environmental Biology
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• v.36 no.4
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• pp.471-478
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• 2018

The purpose of this study was to investigate effects of reusable organic wastes, on soil organisms and chemical properties. Bone meal, de-oiled cake, and oyster shell were applied and abundances of soil organisms were measured at 3 and 15 months, after treatment in soil under sawleaf zelkova cultivation. Soil organisms are affected, by the quality of applied organic wastes. Abundance of microorganisms was higher in oyster shell treated soils than in untreated soils, suggesting that soil pH is a driving force, altering abundance and structure of the microbial community. Increase in abundance of bacterivorous nematodes was observed under treatment with bone meal and de-oiled cake, but there was slight change in abundance of fungivorous and herbivorous nematodes. Abundance of herbivorous nematode was positively correlated with plant growth, at 15 months after treatment. Response of microarthropods in the Collembola and Oribatida was not apparent. Abundances of primary consumers were not significantly correlated with microorganisms. This study suggested that organic waste influences soil organisms primarily by altering soil chemical properties and bottom-up effects may not occur in trophic reactions.

• (The) Research of the performance art and culture
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• no.35
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• pp.203-227
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• 2017

In this thesis, I examined the characteristics of modernism in this work, focusing on the melodrama "Sarang ye soggo donye wulgo(means 'Crying in Love and Crying for Money')", which was popular in the 30s when the consciousness of modernity was overflowing. There has never been an example of a connection between modernism and drama in the 1930s. The characteristic of modernism is clearly embedded in the representative drama of "Sarang ye soggo donye wulgo(means 'Crying in Love and Crying for Money')" at that time. In the title "Sarang ye soggo donye wulgo(means 'Crying in Love and Crying for Money')". 'Don' reveals modern elements. 'Love' can be seen as revealing melodramatic elements. The flair of modern art, which is a background to reveal modern elements, is spread throughout the works. Hongdo fails to complete the relationship with his family, as well as with the couple. It became a person who accepted the modernistic phenomenon by showing the domination of matter. While the typical method of constructing conflicts in the pre-modern narrative works is horizontal and sequential, it can be said that it was in the form of a train station, while the post-modern era of narrative conflict formation from the 30s forms a plurality of conflicts simultaneously, can do. The fear of the ordinary people who see the reality that urban and western values are already rampant by attempting new contents that lead the change of values in "Sarang ye soggo donye wulgo(means 'Crying in Love and Crying for Money')" became a factor to transfer into internal conflict again.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.4
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• pp.335-341
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• 2020

This study investigates the association of the AFB stain with the cycle threshold (Ct) value of the Cobas TaqMan MTB test (CTM test, Roche Diagnostics, Basel, Switzerland), and it establishes the base data for semi-quantitative identification of M. tuberculosis by the Ct value. CTM test were simultaneously conducted on 8,389 specimens submitted to the Samsung Medical Center from January 2015 to December 2015, and the results were analyzed and compared retrospectively investigates the association of the AFB stain with the Ct value of the CTM test, and it establishes the base data for semi-quantitative identification of M. tuberculosis by the Ct value. The Ct values for 135 positive specimens of the CTM were inversely correlated with the AFB stain (rs=-0.545, P<0.01). When the Ct value of the CTM test and the time to positivity (TTP) of the mycobacteria cultures were verified based on the AFB stain, they were found to have a positive correlation (rs=0.136, P<0.01). The negative correlation between the CTM test and the AFB stain grade was demonstrated. The clinical significance was verified by applying these criteria to the clinical results. The semi-quantitative criteria of this study can be used to facilitate the rapid isolation of patients with active tuberculosis and infection control in the hospital.

• Korean Journal of Poultry Science
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• v.51 no.2
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• pp.117-125
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• 2024

This study aimed to find genetic markers for breed-independent identification of early- and late-feathering chickens. We explored the novel sequences of the ev21-K locus associated with late-feathering and investigated its characterization. Additionally, the genetic transmission pattern of the identified sequences were investigated to understand its potential application in auto-sexing lines. A total of 707 chickens from 5 chicken breeds were employed for the study. The ev21-K locus was identified through a comparative analysis of the ev21 gene and the K gene related to feather development. For analysis of identified loci, specific primers for the target sequences were prepared and polymerase chain reaction (PCR) was performed to obtain the products, and then their nucleotide sequences were analyzed. Crossbreeding tests of early-feathering and late-feathering chickens were conducted to examine the genetic transmission patterns of the identified sequences. The results showed that the identified 230 bp ev21-K locus, which named as ev21-related K specific sequences were 99% homology with the ev21 gene. PCR analysis confirmed its presence exclusively in late-feathering chickens. Comparative analyses across tissues, breeds, and ages demonstrated the sequences consistency in identifying late-feathering chickens. Genetic transmission patterns were investigated through crossbreeding tests, revealing sex-linked inheritance and consistent segregation with feathering phenotypes. The inheritance patterns of the ev21-related K specific sequences demonstrated that this locus follows the typical Mendelian inheritance pattern as a dominant gene. In conclusion, the novel sequences of ev21-K locus were a reliable molecular marker for identifying early- and late-feathering chickens across breeds.

• Trans-
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• v.7
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• pp.19-48
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• 2019

This study was described to examine the meaning of the media exhibition work <70mK>, which aims to capture the topography of the collective consciousness of the Korean people through large-scale interviews. <70mK> edits and organizes interview images of individual beings in mosaic-like layouts and forms, creating video exhibitions and holding exhibitions. The objects in the split frame show the continuity of differences that reveal their own thoughts and personalities. This is a synchronic and conscious collective typology in which the intrinsic nature of the individuals is embodied in a simultaneous and holistic image. Interview images reveal their own form as a actual being and convey the intrinsic nature of one's own as oral information. <70mK> constructs a new individualization by aesthetically structuring the forms and information of life individuals in the extension of a specific group. The beings in the frame are not communicating with each other and are looking straight ahead. it conveys to visitors their relationship and personality as the preindividual reality. It is the repetitive arrangement and composition of heterogeneity and difference that each individual shows, and is a chain operation that includes collective identity behind it. <70mK> constructs the direct images and sounds of individual interviewee, creating a new form of information transfer called Video Art Exhibition. This makes metaphors and perceptions of the meaning and process of transindividual relationships and the meaning of psychic individuation and collective individuation. This is an appropriate case to explain with modern technology and individualization of Gilbert Simondon thought together with the meaning of becoming and relation of individualization. The exhibition space constructed by <70mK> is an aesthetic methodology of the psychic and collective meaning and its relationship to a particular group of individuals through which they are connected. Simondon studied the meaning of the process of individualization and the meaning of becoming, and is a philosopher who positively considered the potential of modern technology. <70mK> is a new individual as structured and generated ethical reality mediated by modern technology mechanisms and network behaviors. It is an case of an aesthetic and practical methodology of how interviews function as 'transduction' in the process of individualization in which technology is cooperated. The direct images and sounds of <70mK> are systems in which the information of life individuals is carried, amplified, accumulated and transmitted. It is also a new individual as a psychic and collective landscape. It is a newly became exhibition art work through the multiple individualization, and is a representation of transindividual meanings and process. The media exhibition art of individualized metastable states leads to new relationships in which viewers perceive the same preindividual reality and feel affectivity. The exhibition space of <70mK> becomes a stage for preparing the actual possibility of the transindividual group beyond the representation of the semantic function.

• Clinical and Experimental Pediatrics
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• v.49 no.9
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• pp.977-982
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• 2006

Purpose : There has been an increasing amount of literature concerning the association between Mycoplasma pneumoniae and asthma pathogenesis. Interleukin(IL)-6 stimulates the differentiation of monocytes, and can promote Th2 differentiation and simultaneously inhibit Th1 polarization. IL-8 is a potent chemoattractant and, it has been suggested, has a role in asthma pathogenesis. Nitric oxide (NO) synthesized by airway epithelium may be important in the regulation of airway inflammation and reactivity. Vascular endothelial growth factor(VEGF) has been reported to be a mediator of airway remodeling in asthma. We investigated the effects of M. pneumoniae on IL-6, IL-8, NO and VEGF production in human respiratory epithelial cells. Methods : A549 cells were cultured and inoculated with M. pneumoniae at a dose of 20 cfu/cell. After infection, the presence of M. pneumoniae in epithelial cell cultures was monitored by immunofluorescence and confirmed by polymerase chain reaction(PCR) detection. IL-6, IL-8 and VEGF were determined by an enzyme-linked immunosorbent assay and reverse transcriptase-polymerase chain reaction. NO was measured using the standard Griess reaction. Results : In A549 cells, M. pneumoniaeinduced IL-6, IL-8, NO and VEGF release in time-dependent manners. It also induced mRNA expression of IL-6, IL-8 and VEGF in similar manners. Conclusion : These observations suggest that M. pneumoniae might have a role in the pathogenesis of the allergic inflammation of bronchial asthma.

• Journal of fish pathology
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• v.28 no.2
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• pp.63-69
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• 2015

The biological characteristics of Bacillus sp.CPB-St as a probiotic strain to control fish streptococcosis was determined. Based on 16S rRNA sequencing, Bacillus sp.CPB-St was identified as Bacillus pumilus and named B. pumilus CPB-St (Abbreviated as CPB-St). Growth inhibitory activity of CPB-St against Streptococcus spp. was examined at three different incubation temperatures ($20^{\circ}C$, $25^{\circ}C$, and $30^{\circ}C$) and three culture media (NA, TSA, and BHIA) based on the diameter of inhibition zone. Its activity (inhibition zone of 11~29 mm) at $20^{\circ}C$ was higher than that (12~21 mm) at $30^{\circ}C$. Its activity (29 mm) in NA media was the same as that (29 mm) in TSA media. However, it was higher than that (22 mm) in BHIA media. The inhibitory activity of CPB-St against Streptococcus spp. was high at pH7. However, its activity was the same at salinity of 0.5% to 3%. CPB-St showed maximum growth after incubation at $25^{\circ}C$ for 48 h. To use CPB-St as probiotics, settlement studies in fish intestine and its efficacy through feeding are needed. CPB-St was highly resistant to gastric juice at pH4 and flounder's bile salt as well as deoxycholic acid at $300{\mu}g/ml$. CPB-St showed optimal viability in 1% NaCl. It showed similar growth in 0% to 7% NaCl. CPB-St could tolerate $-20^{\circ}C$ and $-70^{\circ}C$ for 45 min. There was no difference in the growth of the strain between room temperature and $4^{\circ}C$. Fish diet supplemented with CPB-St could be stored at low temperature without cell loss. Therefore, CPB-St might be used as probiotics to control streptococcosis of fish.

• The Korean Journal of Mycology
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• v.17 no.1
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• pp.1-6
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• 1989

The mycelia of Pyricularia oryzae were treated with enzyme solution mixture consisting of Driselase, ${\beta}-Glucuronidase$, Cellulase, and Macerozyme R-10 for the production of protoplasts. More protoplasts were formed from mycelia of race KJ 101 of P. oryzae than that of race KI 315a in the enzyme mixtures. The number of protoplasts was decreased in the untreated control three hrs after the enzyme treatment, whereas the number was increased in the treatments with 10, 50 and 100 mM 2-mercaptoethanol, respectively. The number of protoplasts increased to reach maximum at five hrs after treatment of 10 mM 2-mercaptoethanol, but the least was found in 200 mM. The protoplasts of P. oryzae in a liquid medium containing 2.5% yeast extract, and 2% dextrose reverted to the mycelia after five hrs shaking incubation at $27^{\circ}C$. Some protoplasts produced yeast like buds and the buds were developed to irregularly shaped chains of cell protruded a germ tube like hypha from the distal cell. Once in a while a germ tube like hypha protruded directly from the protoplasts. Except in the first type of reversion, other protoplasts reverted to the normal mycelia. The reversion frequency was highest on PDA with stabilizer of 0.6 M KCl. No reversion of protoplasts occurred on water agar regaardless oftreaatments.