• Journal of fish pathology
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• v.26 no.3
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• pp.255-263
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• 2013

The innate immune response is fundamental defense response of vertebrates and invertebrates. Especially, the innate immune response important for larvae that lack of resistance to infectious diseases in the early stages. Galectin is one of the kinds of lectin and presents in the fish mucous that involves innate immune response. Galectin have been studied from various fishing species, but expression analysis of galectin is still unclear during early developmental stage in olive flounder. In this study, we investigated gene expression of galectin-1 from various developmental stage and tissues. We excised several tissues including the muscle, fin, eye, gill, brain, stomach, intestine, kidney, spleen and liver from adult olive flounder and confirmed gene expression of galectin-1 using RT-PCR and quantitative real-time PCR. Expression of galectin-1 was significantly higher in muscle, stomach and intestinal tissue than other tissue in adult fish (5 and 29 months). Also, galectin-1 gene was detected from 0 DAH and gradually increased to 35 DAH and since then decreased after stomach development period. Induction of galectin-1 during the early developmental stage suggest that muscle, fin and eye tissue is formed and begins the secretion of galectin this period. In addition, increased expression levels at 35 DAH suggest that due to complete formation of stomach and intestine, increase of secretion and activation of enzyme. This study shows that expression of galectin-1 during early developmental stages and adult period in olive flounder and can be expect that galectin-1 play essental role in the innate immune system throughout the whole life time. Galectin-1 is primary barrier such as skin and digestive tissue against pathogen infection, also digestive tract developmental period is important for pathogen invasion can be expected that it will serve. Mass mortality due to the disease in seed production is continuing damage, therefore these result will be meaningful about infectious disease during early developmental stages as a basic data for the study.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.5
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• pp.957-964
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• 2000

To assess trans fatty acids (tFAs) intake of a girls' high school students in Pusan, dietary history and quantitative food frequency questionnaire (FFQ) were surveyed and analyzed with a total subjects of 274, 268 in summer and winter respectively. It was shown that 38.0% of subjects which was the highest percentage, had snack twice a day in frequency of snack intake. The most favorite snack of subjects was ice cream (46.4%), the preference for milk and dairy products was considerably high. 43.4% of subjects responded to once a month in frequency of dining out. They preferred grilled pork (39.1%), hamburger (36.9%), grilled beef (26.6%) in order. It was also appeared that they preferred fast food. In the case of eating bread, 8.0% of subjects ate bread and margarine, 7.1% butter. Generally, the subjects preferred margarine to butter. tFAs intake for each person per day was $4.24{\pm}0.18\;g$. There was significant difference in tFAs intake according to parents income (p<0.05), frequency of snack intake (p<0.001) and that of dining out (p<0.05). tFAs intake produced naturally (N-tFAs) was $0.86{\pm}0.00\;g$ which corresponded to 20.3% of total tFAs intake per day. tFAs intake produced artificially (H-tFAs) in the process of hardening oil was $3.38{\pm}0.14\;g$, equaled to 79.7% of total tFAs intake per day. There was significant difference between H-tFAs intake for each person per day (p=0.000)

• Journal of agriculture & life science
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• v.45 no.3
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• pp.69-79
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• 2011

This study was conducted to estimate effects of by-products of medical herbs replacing rice straw on in vitro fermentation characteristics. Each trial was composed of five treatments including medical herbs : rice straw (%) = 20 : 80 (T1), 40 : 60 (T2), 50 : 50 (T3), 100 : 0 (T4) and the control. Each treatment had eight fermentation times (3, 6, 9, 12, 24, 36, 48 and 72 hours) with three replications. The gas production and DM degradation were significantly (P<0.05) increased by supplementation, especially T4, during the whole fermentation periods. Methane production increased along with addition of by-products similar to the gas production and DM degradation. The pH values ranged from 5.39 to 6.80 and were significantly (P<0.05) decreased by supplementation of by-products of medical herbs. Microbial growth rates reached the peak at between 36 and 48h, thereafter tended to decrease. Although there were no significant differences in the enzyme activities, there was a tendency of increase in T4 treatment. From above results, the replacement levels, particularly 100% replacement of rice straw by by-products of medical herbs, resulted in improving the in vitro fermentation characteristics such as increasing gas production, microbial growth and DM degradation. Also it may help digestion by increasing enzyme activities.

• Korean Journal of Remote Sensing
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• v.14 no.3
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• pp.296-306
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• 1998

Airborne MSS data is regarded as a potentially effective data source for the measurement of water quality and for the environmental change of water bodies. In this study, we measured the radiance reflectance by using multi-spectral image of low resolution camera(LRC) which will be reached in the multi-purpose satellite(KOMPSAT) to use the data in analyzing water pollution. We also investigated the possibility of extraction of water quality factors in water bodies by using high resolution remote sensing data such as Airborne MSS. Especially, we tried to extract environmental factors related with eutrophication such as chlorophyll-a, suspended sediments and turbidity, and also tried to develop the process technique and the radiance feature of reflectance related with eutrophication. Although it was difficult to explicitly correlate Airborne MSS data with water quality factors due to the insufficient number of ground truth data. The results were summarized as follows: First, the spectrum of sun's rays which reaches the surface of the earth was consistent with visible bands of 0.4${\mu}{\textrm}{m}$~0.7${\mu}{\textrm}{m}$ and about 50% of total quantity of radiation could be found. The spectrum was reached highest at around 0.5${\mu}{\textrm}{m}$ of green spectral band in visible bands. Second, as a result of the radiance reflectance Chlorophyll-a represented high mainly around 0.52${\mu}{\textrm}{m}$ of green spectral band, and suspended sediments and turbidity represented high at 0.8${\mu}{\textrm}{m}$ and at 0.57${\mu}{\textrm}{m}$, respectively. Finally, as a result of the water quality analysis by using Airborne MSS, Chlorophyll-a could have a distribution image after carrying out ratio of B3 and B5 to B7. Band 7 was useful for making the distribution image of suspended sediments. When we carried out PCA, suspended sediments and turbidity had distributions at PC 1 and PC 4 which are similar to the ground data. Above results can be changed according to the change of season and time. Therefore, in order to analyze the environmental factors of water quality by using LRC data more exactly, we need to investigate the ground data and the radiance feature of reflectance of water bodies constantly. For further studies, we will constantly analyze the radiance feature of the surface of water in wafter bodies by measuring the on-the-spot radiance reflectance and using low resolution satellite image(SeaWiFS). We will also gather the data of water quality analysis in water bodies and analyze the pattern of water pollution.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.872-879
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• 2010

Layered copper hydroxides [LCHs, $Cu_2(OH)_3{\cdot}NO_3$] has the agricultural potentials as a fungicide because of its high crystallinity, excellent anion exchange capacity, and its regular layered particle size. The study, for the first time, has synthesized LCHs in highly concentrated solution and evaluated its physicochemical properties including the crystallinity and suspension stability. Optimal synthetic condition of LCHs was determined by crystallinity and stability of suspension as follow; 1) concentrations of $Cu(NO_3)_2$ and NaOH solutions were 3.0 M respectively, 2) reaction temperature and solution pH were $25^{\circ}C$ and 6.0, respectively, and 3) aging time after reaction was 2hr. Crystallinity of LCHs enhanced with increase in pH up to 9.0. Whereas, stability of suspension was decrease by increase in crystal size. Especially, increase in reaction temperature decreased stability of suspension. XRD patterns and SEM images exhibited that LCHs had regular layered particle size with 0.2~0.8 ${\mu}m$ and high crystallinity in optimal synthetic condition. The particle size was increased with increase in reaction temperature and pH. These results showed that LCHs synthesized in highly concentrated solution exhibited high stability of suspension as well as high crystallinity suitable to their potential as a fungicide.

• Journal of Mushroom
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• v.19 no.3
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• pp.166-175
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• 2021

In this study, monokaryons of "Heukari" (Pleurotus ostreatus) and "Hosan" (Pleurotus pulmonarius) were separated to remove the cell wall, and a cross-species protoplast fusion was developed through chemical treatment with polyethylene glycol. The protoplast-fused PF160306 and PF160313 strains have a culture period of 10 and 2 days shorter than that of the "Heuktari" and "Hosan" cultivars, respectively. Furthermore, the growth of the strains was faster than that of the existing cultivars. The yield was 135.9 g per bottle, which was approximately 8% higher than that of the commercially available "Hosan" cultivar; however, it was not statistically significant. A growth survey was conducted after treatment at five temperatures (15, 18, 21, 23, and 25℃). The growth of the strains accelerated with the increase in temperature. However, at 21℃, the yellow color of pileus was the brightest. Band pattern, assessed using URP Primer 7, was similar to that of the "Hosan" cultivar. The DPPH radical scavenging capacity and polyphenol content were 62.5% and 43.5 mg/mL, respectively, for "Sunjung" and 65.7% and 49.9 mg/mL, respectively, for PF160313. Furthermore, the antihypertensive activities of the "Sunjung" cultivar and PF160313 were similarly high at 74% and 75%, respectively. In conclusion, cross-species hybridization via the protoplast fusion technique can be used for obtaining primary data for mushroom breeding to develop new varieties. In addition, the protoplast fusion technique might aid in expanding the market for yellow mushrooms.

• Korean Journal of Environment and Ecology
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• v.35 no.5
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• pp.525-547
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• 2021

The main purpose of this study is to examine the use of plant species in ecological restoration projects. To this end, planting drawings from 58 sites that had completed the return of the ecosystem conservation cooperation fund for the past six years were collected and analyzed. The analysis used the construction completion and design drawings to determine the overall selection status and analyze frequency by classifying planted plants into wild and cultivated plants by nature, size, vegetation climate, and upland and wetland habitat. The investigation and analysis process found many cases of wrong plant names, so an analysis was also performed on the matter. In the 58 investigation sites, 282 plants were used for planting: 91 tree species, 69 shrub species, 11 vine species, and 111 herbal species. The most commonly used plant species was Spiraea prunifolia f. simpliciflora, followed by Sorbus alnifolia, Quercus acutissima, Zoysia japonica, Callicarpa dichotoma, and Weigela subsessilisin that order. The most commonly used tree species was Sorbus alnifolia,followed by Quercus acutissima, Zelkova serrata, Chionanthus retusus, and Cornus officinalis, in that order. The most commonly used shrub species was Spiraea prunifolia f. simpliciflora, followed by Weigela subsessilis, Callicarpa dichotoma, Rhododendron yedoense f. poukhanense. and Euonymus alatusin that order. The most commonly used herbal plant species was Zoysia japonica, followed by Dendranthema zawadskii var. latilobum, Aster koraiensis, Miscanthus sacchariflorus, and Pennisetum alopecuroidesin that order. In the analysis by vegetation climate, Spiraea prunifolia f. simpliciflora, Callicarpa dichotoma, and Sorbus alnifoliawere most used in that order in both the temperate central and the warm temperate forest zones, but the pattern does not properly reflect the climate characteristics. In the analysis by habitat, Miscanthus sacchariflorus and Lythrum salicariawere most used in the wetland. In particular, the ratio of wild plants to cultivated plants was 76% to 24%, indicating the ratio of selecting cultivated plants was high. The names of plants on the drawings were mostly common names that did not appear in the Korea National Arboretum or the National Species List of Korea. It is necessary to use proper plant names in the future. Regarding the use of planting plants for ecological restoration, it is necessary to adopt the approach of diversifying selected plants, selecting plants according to characteristics of climate zones, and lowering the specifications of plants used for ecological restoration. Moreover, it is important to fully understand the ecological characteristics of wetland plants and minimize the ratio of using cultivated plants to ensure the plant selection centered on wild plants.

• Korean Journal of Food Science and Technology
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• v.53 no.4
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• pp.375-381
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• 2021

This study investigated the physicochemical properties and chemosensory characteristics of radish samples. Brix results in Matdong-radish showed the highest value (1.5±0.1%), and Mansahyungtong-radish showed the lowest. In terms of salinity, Mansahyungtong-radish had the highest value (1.2±0.1%), and there were no significant differences among samples except Mansahyungtong-radish. In pH analysis, Cheongjunggowon-radish had the highest value at 6.69±0.02. The pH in Mansahyungtong-radish showed the lowest value at 6.10±0.01. In the electronic tongue, sourness was high in the Seoho-radish (8.2), and saltiness was high in the Matdong-radish (8.1). Umami was high in the Seoho-radish (8.3), and sweetness was high in the Matdong-radish (8.1). In the electronic nose, sulfur-containing compounds were high in all the samples. Methanethiol, which represented the odor description of cabbage, garlic, and sulfurous, was abundant in sulfur-containing compounds. Multivariate analysis using physicochemical and chemosensory properties can be used as a database for the food industry.

• Korean Journal of Remote Sensing
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• v.37 no.2
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• pp.275-290
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• 2021

Sulfur dioxide (SO2) in the atmosphere is mainly generated from anthropogenic emission sources. It forms ultra-fine particulate matter through chemical reaction and has harmful effect on both the environment and human health. In particular, ground-level SO2 concentrations are closely related to human activities. Satellite observations such as TROPOMI (TROPOspheric Monitoring Instrument)-derived column density data can provide spatially continuous monitoring of ground-level SO2 concentrations. This study aims to propose a 2-step residual corrected model to estimate ground-level SO2 concentrations through the synergistic use of satellite data and numerical model output. Random forest machine learning was adopted in the 2-step residual corrected model. The proposed model was evaluated through three cross-validations (i.e., random, spatial and temporal). The results showed that the model produced slopes of 1.14-1.25, R values of 0.55-0.65, and relative root-mean-square-error of 58-63%, which were improved by 10% for slopes and 3% for R and rRMSE when compared to the model without residual correction. The model performance by country was slightly reduced in Japan, often resulting in overestimation, where the sample size was small, and the concentration level was relatively low. The spatial and temporal distributions of SO2 produced by the model agreed with those of the in-situ measurements, especially over Yangtze River Delta in China and Seoul Metropolitan Area in South Korea, which are highly dependent on the characteristics of anthropogenic emission sources. The model proposed in this study can be used for long-term monitoring of ground-level SO2 concentrations on both the spatial and temporal domains.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.2
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• pp.61-67
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• 2019

This study was conducted to investigate the effect of supplementary feeding levels on livestck and forage productivity and grazing intensity in Elk stags (Cervus canadensis). A fifteen 2-year-old Elk stags about 195 kg were randomly assigned to one of three dietary treatments (five animals per treatment). The dietary treatments consisted of a feeding concentrate of 1.0% of body weight (T1), 1.5% of body weight (T2) and 2.0% of body weight. Total dry matter intake (TDMI) was increased with increased with an increasing supplementary feeding levels. Average daily gain (ADG) were significantly increased with an increasing supplementary feeding levels (p<0.05) and reached a maximum on July and was lower in spring than autumn. The velvet antler production was no differences among treatment groups. Forage productivity of pasture and crude protein content were highest on May and decreased thereafter, however, crude fiber content was the reversed. The grazing intensity of Elk stags was increased in spring (38 to 59 head per ha) than summer and autumn (13 to 32 head per ha). The average grazing intensity of Elk stags ranged from 21 to 34 head per ha, which is affected by supplementary feeding levels. This result suggests that feeding supplementary diet at 1.5 % of body weight was needed to maintain the stable wight gain in antler growing periods and control the proper grazing intensity of Elk deer stags.