• Food Science of Animal Resources
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• v.22 no.1
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• pp.13-19
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• 2002

The natural sources extracted from green tea and pine needle were utilized to investigate the effects of extracts on free radical reaction, lipid oxidation and nitrite scavenging ability. The degree of lipid oxidation is very sensitive to kinds of oil emulsion reacted with iron sources and oxygen species. The antioxidants of extracts from green tea and pine needle were different depending on concentration of extracts, which were a lower TBARS value in 0.3% extracts concentration, compared to 0.1% extracts concentration. And also, the binding ability on iron sources was superior in hot water extracts, but oxygen scavenging ability was the lowest TBARS values in ethanol extracts. The hydroxyl radical scavenging ability of green tea and pine needle extracts had a little low TBARS value in 0.1% and 0.3% extracts concentration in deoxyribose. The ethanol extracts of pine needle were higher than those of hot water extracts on the basis of Fe$\_$2+/ ion content. The ascorbic acid content of green tea showed 14.3 mg/100g in hot water extracts and 16.7 mg/100 g in ethanol extracts. Electron donating ability of extracts showed difference depending on extracts concentration, which were higher in ethanol extracts than those of hot water extracts. The nitrite scavenging effects were tended to be different depending on pH value, however they were decreased overall as pH value was increased. Especially, the nitrite scavenging ability of 0.3% extracts from green tea and pine needle were the mosts effective in pH 1.2 and pH 3.0, which were showed 95% nitrite scavenging ability.

• Korean journal of applied entomology
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• v.50 no.3
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• pp.227-233
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• 2011

Crysochroa fulgidissima (Bidan-beole, Spanish fly) is traditionally used as a crude drug and insecticide in the East Asia and Korea, respectively. This study investigated the effect of ethanol extract of C. fulgidissima on the NO production activity. The C. fulgidissima extract was a potent inducer of NO production in CPAE cells and a stimulator of endothelial nitric oxide synthase in a dose-dependent manner. This study also evaluated the anti-inflammatory activity of this extract by determining the level of ICAM-1, VCAM-1, and prostaglandin $E_2$ from HUVEC cells. Although C. fulgidissima extract was a potent inducer of NO production in the CPAE cells, it showed weak inhibitory effects on vascular endothelial growth factor (VEGF) production in HUVEC cells. HPLC and GC-MS analysis of the ethanol extract of C. fulgidissima revealed the presence of cantharidin.

• Analytical Science and Technology
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• v.17 no.1
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• pp.37-44
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• 2004

An alcohol oxidase from Hansenula polymorpha was strongly induced when cells were grown with 0.5% methanol supplementation as the carbon source. The induced Hansenula polymorpha alcohol oxidase was purified to electrophoretic homogeneity by using DEAE-Sephacel and Mono Q column chromatographys. The enzyme oxidized mainly primary aliphatic alcohols and exhibited high substrate specificity towards ethanol and methanol. The activity of the enzyme optimally proceeded at pH 8.5 and $50^{\circ}C$. The midpoint of the temperature-stability curve for the enzyme was approximately $52^{\circ}C$ and the enzyme was not completely inactivated even at $65^{\circ}C$ temperature. The enzyme showed resistance toward detergents and highly stable over 7 weeks of storage condition. This Hansenula polymorpha alcohol oxidase may be useful for the enzymatic determination of alcohol and for the industrial production of alcohols and aldehydes.

• Korean Journal of Plant Resources
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• v.37 no.2
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• pp.120-129
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• 2024

Alcoholic liver disease (ALD) is a significant risk factor in the global disease burden. The stem bark of the Betulaceae plant Alnus japonica, which is indigenous to Korea, has been used as a popular folk medicine for hepatitis and cancer. However, the preventive effect of Alnus japonica leaf extracts on alcohol-related liver damage has not been investigated. The objective of this study was to investigate the hepatoprotective effects of the extracts of Alnus japonica leaf (AJL) against ethanol-induced liver damage in HepG2/2E1 cells. Treatment with AJL significantly prevented ethanol-induced cytotoxicity in HepG2/2E1 cells by reducing the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). This protective effect was likely associated with antioxidant potential of AJL, as evidenced by the attenuation of reactive oxygen species (ROS) and malondialdehyde (MDA) production and restoration of the depleted glutathione (GSH) levels in ethanol-induced HepG2/2E1 cells. Our findings suggest that FCC might be considered as a useful agent in the prevention of liver damage induced by oxidative stress by increasing the antioxidant defense mechanism.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2000.11a
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• pp.382-383
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• 2000

산업이 발달함에 따라 휘발성 유기 화합물(VOC : Volatile Organic Compound)의 배출이 급증하고 있다. 이러한 VOC들은 대기중에서 산화물과 공존시 태양광의 작용을 받아 광화학반응을 일으켜 오존등의 광화학 산화성 물질을 생성시켜 광화학 스모그를 유발시키고 일부 VOC는 그 자체로서도 암을 유발시키는등 인체에 좋지 않은 영향을 미치게 된다. 이러한 VOC중 상당수는 대기중에 미량만 존재하게 되더라도 악취를 유발시켜 불쾌감을 주게된다. (중략)

• Proceedings of the KAIS Fall Conference
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• 2002.05a
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• pp.264-266
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• 2002

원자력 발전소의 2차계통수에는 pH를 조절하여 부식을 억제하기 위해 pH제어제로 약염기성 화학물질을 첨가하고 있다. pH 제어제로 암모니아를 사용하였으나 pH가 낮아 부식생성물이 생성되어 증기발생기의 전열관에 슬러지의 퇴적으로 전열관의 부식이 촉진되므로 pH 제어제를 에탄올아민으로 바꿈으로 슬러지의 생성 및 이동을 억제하고 있다. 그러나 에탄올아민은 암모니아와 물리화학적 성질이 다르므로, 증기발생기에 유입되는 부식생성물의 용해와 흡착, 이온성 불순물의 잠복현상에 미치는 영향이 다르다. 본 연구는 암모니아와 에탄올아민이 온도가 점차 높아짐에 따라 부식생성물에 대찬 용해와 흡착, 이온성불순물의 잠복현상에 미치는 영향을 조사하였다. 이 실험의 결과로 2차 계통수의 pH 제어제는 ETA가 암모니아보다 증기발생기 슬러지의 철산화물에 더 흡착되어 더 많이 용해되어 퇴적된 슬러지 양을 감소시키므로, 슬러지에 흡착된 불순물의 양을 감소시저 잠복 현상을 억제할 것으로 조사되었다.

• Food Science and Preservation
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• v.30 no.2
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• pp.334-346
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• 2023

The antioxidant ability of 80% ethanolic extract of nutmeg seed (NM80) was evaluated using in vitro assays and bulk oil and oil-in-water (O/W) emulsion matrices. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation radical scavenging, and oxygen radical antioxidant capacity (ORAC) in vitro assays were used to evaluate the antioxidant ability of the extract. The DPPH radical scavenging activities of 25, 50, 100, and 200 ㎍/mL NM80 were 12.5, 20.9, 35.1, and 62.8%, respectively, while the ABTS cation radical scavenging activities were 2.7, 6.5, 30.5, and 29.8%, respectively, demonstrating a dose-dependent effect. The ORAC value was significantly higher at an NM80 concentration of 25 ㎍/mL than the positive control (p<0.05). The conjugated dienoic acid (CDA), ρ-anisidine, and tertiary butyl alcohol values in 90-min-heated corn oil containing 200 ppm of NM80 were significantly reduced by 3.26, 16.94, and 17.34%, respectively, compared to those for the sample without NM80 (p<0.05). However, the headspace oxygen content and CDA value in the O/W emulsion containing 200 ppm of NM80 at 60℃ had 6.29 and 82.85% lower values, respectively, than those for the sample without NM80 (p<0.05). The major volatile compounds of NM80 were allyl phenoxyacetate, eugenol acetate, and eugenol. NM80 could be an effective natural antioxidant in lipid-rich foods in bulk oil or O/W emulsion matrix.

• Journal of Life Science
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• v.17 no.10
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• pp.1330-1335
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• 2007

This study was developed to assess the antioxidative activity of aged black garlic extract on lipid peroxidation and low density lipoprotein (LDL). Antioxidative activity of aged black garlic extract on human low density lipoprotein (LDL) was investigated by monitoring a barbituric acid reactive substance (TBARS). Electron donating ability (EDA) of aged black garlic, ethanol extract was higher than that alliin and water extract. Aged black garlic water and ethanol extracts inhibited the $Cu^{2+}$ mediated oxidation of human LDL in a dose dependent manner at concentration of 10 and $20\;{\mu}g/ml$. Ethanol extract and water extract of aged black garlic almost completely inhibited J774 mediated LDL oxidation in electrophoretic mobility and conjugated diene. These results indicate that aged black garlic might play a protective antioxidant effects on LDL, probably affecting the structural properties for the LDL oxidation.

• Korean Journal of Food Science and Technology
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• v.53 no.3
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• pp.329-333
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• 2021

Subcritical water extraction (SWE) is an eco-friendly extraction method that uses only purified water as a solvent under high temperature and high pressure conditions. In this study, total phenolics, and antioxidant activity were evaluated in Ziziphus jujuba Mill extracts from subcritical water obtained by varying the extraction temperature (110-190℃) and extraction time (1-20 min). Total phenolics was maximized with extraction at 190℃ for 15 min (67.79±3.45 mg gallic acid equivalent/g jujube). The 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity (48.84±4.74%) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free radical scavenging activity (84.75±1.15%) were maximal at extraction conditions of 190℃, for 20 min. All jujube extracts prepared using SWE had higher total phenolics and antioxidant activities than extracts prepared using organic solvent extraction (60℃, 120 min), including methanol and ethanol. SWE could be an excellent alternative to organic solvents for extracting phenolics and antioxidant compounds.

• Food Industry And Nutrition
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• v.7 no.3
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• pp.44-49
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• 2002

본 연구는 retinyl acetate와 $\beta$-carotene보충식이가 에탄올에 의한 산화적 손상에 미치는 영향을 조사하고자 흰쥐에게 비타민 A와 $\beta$-carotene을 보충한 식이를 5주간 급여한 후 에탄올을 급성으로 투여하여 혈청과 간 조직에서 생성되는 지질과 지질과산화물 함량 그리고 glucose-6-phosphatase 활성도를 측정하였다. 본 실험에서 얻은 결과를 요약하면 체중 증가량은 전 실험군에서 유의적인 차이없이 실험 기간 동안 증가되었으며 사료섭취량과 식이효율을 그리고 간 중량 역시 각 군 모두 유의적인 차이를 나타내지 않았다. 혈청 중성지질 함량은 대조군과 retinyl acetate 보충군에서 높은 함량을 보였으나 통계적인 유의성은 없었다. 총 콜레스테를 함량은 대조군과 $\beta$-carotene 보충군은 비슷한 수준을 나타낸 반면 retinyl acetate 보충군에서는 유의적으로 감소되었다. HDL-콜레스테롤 함량은 대조군에서 가장 높았으며 LDL-콜레스테롤 함량은 유의적이지는 않았으나 retinyl acetate 보충군에서 가장 낮은 함량을 보였다. 동맥경화지수는 전 실험군에서 유의적인 차이는 없었으나 retinyl acetate 보충군에서 가장 낮았다. 간 마이크로솜 내에서 지질과산화물 함량은 재도군에 비하여 $\beta$-carotene 보충군에서 유의적으로 감소되었다. Retinyl acetate와 $\beta$-carotene 보충군 사이에서는 유의적인 차이가 없었으나 급성적인 에탄올 투여시에 $\beta$-carotene의 보충 급여가 지질과산화반응에 방어효과가 있는 것으로 나타났다. 간 조직중의 glucose-6-phosphatase 활성도는 비타민을 정상적으로 공급시킨 대조군이 retinyl acetate나 B-carotene 보충군에 비하여 유의적으로 높았으며 retinyl acetat와 B-carotene 보충군 사이에는 유의적인 차이가 없었다.