This study was performed to determine the complaint rates of musculoskeletal symptoms and its association with related factors among manufacturing workers. The self-administered questionnaires were given to 856 male with 50 or less employees in manufacturing industries. As a results, complaint rates of work-related musculoskeletal symptoms of the body sites was 61.2% for shoulders, 55.4% for low back, 55.3% for neck, 38.6%, for legs/feet, 32.2% for hands/wrists/fingers and 26.9% for arms/elbows. In logistic regression analysis of significant factors related with musculoskeletal symptoms of body sites, neck was related with marital status, subjective health status, smoking, drinking coffee and occupational stress, shoulders was related with job tenure, shift work, visiting out-patient department and occupational stress, arms/elbows was related with sleeping time, experience of sick absence and occupational stress, hands/wrists/fingers was related with age, smoking, drinking coffee, working hour, job position, job tenure, visiting out-patient department and occupational stress, low back was related with educational level, BMI, sleeping time, job position, job tenure, visiting out-patient department and occupational stress and legs/feet was related with marital status, job position, job tenure, history of hospitalization and occupational stress.