• Korean journal of applied entomology
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• v.44 no.2
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• pp.109-114
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• 2005

This study was conducted to investigate the life cycle of Tipula latemarginata Alexander in Korea. The field surveys for the life cycle of the species were carried out at the two sites of Neaseong Stream area in Bongwha County from January 2001 to December 2002. Also many individuals of the species were reared in laboratory to investigate the life cycle. T latemarginata appeared to have three generations a year under the rearing conditions at room temperature. All the processes of life cycle of the species, when reared at room temperature from September 2001 to March 2002, were as follows: Eggs usually hatched between 4 and 10 days after oviposition. First instar larvae grew rapidly and molted to the second instar in 7-9 days. Second instar larvae spent 5-7 days for next molting and third instar period lasted approximately 8-12 days. Fourth instar larvae spent 6 weeks to 5 months for pupation. Especially the duration of fourth instar larval existence was greatly lengthened and served as the overwintering stage. The duration of pupal stage was 6-10 days. In the field, T. latemarginata also appeared to have three generations a year at the favorable habitats.

• Korean journal of applied entomology
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• v.35 no.2
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• pp.146-152
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• 1996

The adult of the aphidophagous ladybeetle, Harmonia axyridis, was reared by feeding an artificial diet (chicken's 1iver:sugar = 5:2, v/w). The adult ladybeetle used was from pupae collected in the field in midJuly. Yolk protein production, fecundity and longevity of the adult female were checked under 3OoC, 16L:8D. Yolk proteins (Vg3 and Vg4) were detected from the hemolymph of the female 5 days after the emergence. One hundred pairs of female and male were reared separately. First oviposition started from 7 days afier the emergence and laid an average of 163.9k144.0 eggs during lifetime. Seventeen females did not lay eggs at all. Longevity of the female was 60.6k16.9 days. The application of a JH analog, methoprene (2.5 pg), caused the occurrence of yolk proteins in the hemolymph 1 day earlier than that of the control. Weekly application of 2.5 pg of methoprene induced 83.3% from the 30 females to lay eggs within 15 days after the emergence, while the control had 51% only. Methoprene made all females lay eggs, causing the increase in the fecundity. Though the longevity of the female was not effected by the weekly application of methoprene, one time excess dose (12.5 pg) caused high in mortality and low in fecundity.

• Asian Journal of Turfgrass Science
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• v.17 no.1
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• pp.35-44
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• 2003

Popillia quqdriguttata is a serious insect pest in Korean golf courses. The adult feeds on leaves of latifoliate trees and herbaceous plants while the larva feeds on roots of turfgrass. Thus, control of p. quadriguttata adults was tried with registered insecticides in golf courses in Korea. The insecticides used in the experiments were carbaryl + phosalone WP, chlorpyrifos + diflubenzuron WP, chlorpyrifos-methyl EC, deltamethrin EC, etofenprox WP, etofenprox + diazinon WP, etofenprox + tebufenozide EC, fenitrothion EC, imidacloprid SC, pyraclofos WP, and tralomethrin EC. Mortalities of P. quadriguttata were over 95% in carbaryl + phosalone WP, chlorpyrifos + diflubenzuron WP, etofenprox + diazinon WP, and fenitrothion EC at petri dish and in greenhouse. In the field test, corrected mortality of P. quadriguttata was 95.2% and 80.9% in carbaryl 40% +phosalone 20% WP and fenitrothion 50% EC, respectively. Spreading agent enhanced toxic effect of used insecticides to p. quadriguttata adults except carbaryl + phosalon 20% EC. p. quadriguttata adult was firstly observed to feed on leaves of Canna generalis at the Yongwon golf club in Jinhae, Gyeongnam and Prunus ameriaca var. ansu and p. salicina at the Anyang Honest golf club in Gunpo, Cyeonggi. Therefure, host plants off quadriguftata became 29 species of 26 genera in 19 families. Zoysia matreila at the fairway and roughs of Anyang Benest golf club was damaged by p. quadriguttata larvae in Octomber, 2002 and not recovered in April, 2003. Damaged turfgrass became yellowish and wilted and that retarded recovering in the following spring.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.479-484
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• 2012

The occurrence of plum fruit moth, Grapholita dimorpha, has been recently reported in apple orchards in Korea. It has been suspected that G. dimorpha and its related species, G. molesta, may occur simultaneously in other host plants. This study reports the occurrence of G. dimorpha in pear orchards of different localities in Korea. The identification of G. dimorpha was determined by morphological characters and the DNA marker. The cross-trapping of both species may be possible because the major sex pheromone (SP) compositions for the two species are similar. From the monitoring data, G. dimorpha and G. molesta were caught in SP lure traps of G. dimorpha and both species were also caught in SP lure traps of G. molesta. This cross-trapping of G. molesta to a SP lure of G. dimorpha varied significantly among pear orchards in different geographical localities. Furthermore, the occurrence peaks of the two species were not coincidental in all monitored orchards. These suggest that monitoring data obtained from each SP trap of both species in pear orchards may be mixed with two species, which would result in the over-estimation of population density and peak frequency on both species in pear orchards.

• The Journal of the Petrological Society of Korea
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• v.16 no.2 s.48
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• pp.59-72
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• 2007

Kilauea volcano located in the southern part of Hawaii island chains has been formed by continuous and sporadic eruption activities from 16 century to September of 1982. Kilauea volcano was investigated from collecting the samples of basalt to identify the geochemical characteristics of the lavas. Olivine, clinopyroxene, plagioclase, and opaque minerals with glass groundmass are observed. Clinopyroxene formed glomerophopyritic texture with plagioclase and zoning texture was observed in some of plagioclase phenocrysts. Major elements such as $K_2O$, $P_2O_5$, $TiO_2$ increased with respect to MgO. $Al_2O_3$ and CaO show the kink on Marker diagram due to the crystal fractionation of plagioclase and clinopyroxene with plagioclase respectively. Olivine has a wide range of Fo% ranging from 60 to 90, and MnO and FeO in the olivine are decreased with increasing Fo% oppositely increased $SiO_2$, MgO, and NiO. Ni in the whole rock is controlled not only by the mode of olivine but also by the Ni abondance in the olivine.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.528-533
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• 2010

Cage plots were established in 2008 to find the relationship between combination of tended Japanese red pine sapling and the habitation of Monochamus alternatus, a vector for pine wood nematode. Japanese red pine sapling tended at different times were successively put into the cage, and then the adult of Monochamus alternatus was put in June. Presence or absence of the larva was determined November. Sapling tendings were done at seven successive times in 2009, mid- and late February and March, mid-April, May and June. Some saplings were also tended in mid-October of 2008, the previous year of this experiment. The larva was found in all the saplings tended later than mid-March in 2009. No larva was found in the saplings tended in mid-October of 2008 and those in February of 2009. In case of the combination the by-products of mid-February and late March of 2009 and mid-October of 2008, the larva was found in the by-product of mid-February and late March of 2009, but those in mid-October of 2008 was not. When the saplings tended in mid-October of 2008 and mid- and late February of 2009 were put into the cage singly, the larva was found in all the saplings. Results indicated the mixture of the saplings tended at different times affected the habitation of Monochamus alternatus differently.

• The Korean Journal of Mycology
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• v.45 no.1
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• pp.43-53
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• 2017

In this study, we analyzed the densities and taxonomic characteristics of various microorganisms that play important roles in Agaricus bisporus culture medium composting, and examined changes in the levels of decomposition-related enzymes secreted by these microorganisms. Various microorganisms such as thermophilic bacteria, actinomycetes, fluorescent Pseudomonas spp., and filamentous bacteria are closely associated with culture medium composts of Agaricus bisporus. The population densities of microorganisms change, and harmful bacteria disappear during thermophilic composting. Psychrobacter sp., Pseudomonas sp., Bacillus sp., and Pseudoxanthomonas sp. accounted for the highest proportion of bacteria in the culture media during outdoor composting, whereas Bacillus sp. and Psychrobacillus sp. were dominant after pasteurization. Cellulose and hemicellulose enzymes of the microorganisms were important at an early stage of rice straw composting and after decomposition of carbon sources, respectively. Microorganisms that secreted these enzymes were present in the second and third turning stage of composting.

• Research in Plant Disease
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• v.14 no.1
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• pp.43-50
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• 2008

Rhizoctonia blight (large patch) caused by Rhizoctonia solani AG2-2 is one of the major diseases on zoysiagrass in golf courses. In this study, anatgonistic bacteria to R. solani AG2-2 were selected in vitro tests using confrontation bioassay and triple layer agar diffusion method. The most active bacteria, Bacillus subtilis CJ-9 were tested for controlling large patch in pots. Relative Performance Indies (RPI) was used as a criterion for the selection of potential biocontrol agent. B. subtilis CJ-9 showed resistance to major synthetic agrochemicals used in golf course. In field tests at golf course, B. subtilis CJ-9 was more effective in suppression of large patch severity and population development of R. solani AG2-2 in soil than chemical fungicides. B. subtilis CJ-9 could be an alternative to chemical fungicides for eco-friendly management of large patch on zoysiagrass.

• Korean Journal of Microbiology
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• v.41 no.4
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• pp.269-274
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• 2005

We evaluated the PCR assay and two commercialized PCR kits for the detection of mycoplasma in veterinary via live vaccines. The PCR assay could specifically detect all the tested Mycoplasma spp. and Acholeplasma spp., whereas two commercialized PCR kits did not. Also, the specificity of the PCR assay showed that 4 reference strains and 7 field isolates belonging to avian mycoplasma species could be all detected. The sensitivity of the PCR assay was determined using pure cultured Mycoplasma spp. and Acholeplasma spp. with a range of 1 to 100 colony forming units/ml in 9 CFR Mycoplasma broth. To test the availability of the PCR assay for veterinary live viral vaccines, A. laidlawii was artificially inoculated into the swine transmissible gastroenteritis-rota virus combined vaccine and canine parvovirus vaccine, respectively and the sensitivity of the PCR assay was similar with the result of cultured samples. In this study, the PCR assays could be used as rapid and sensitive methods for the detection of mycoplasma in veterinary live viral vaccines.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.4
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• pp.420-427
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• 2019

Scrub typhus is a fever-related disease that is commonly diagnosed during the autumn season. The number of patients affected by this disease continues to increase every year. However, the cause of the disease and the regional differences of its incidence are poorly understood. This study examined the epidemiological features and regional differences in the incidence of scrub typhus. The monthly incidence of cases from all reports rose to more than 90% during the fall season (October~December). Based on the analysis of the 18,851 cases in the epidemiological investigation, 14,777 (78.4%) and 4,074 (21.6%) cases occurred in high and low occurrence areas, respectively. Moreover, there were more female cases than male cases (males: 7,233 [38.4%]; females: 11,618 [61.6%]; P< 0.001). The clinical symptoms, including fever, chills, myalgia, and eschar of the body, were noted in 16,688 (89.1%), 11,533 (61.2%),11,439 (60.7%), and 6,005 (45.4%) cases, respectively. The incidence rates were higher for Gyeongnam, Jeonam, Jeonbuk, and Chungnam provinces. The areas with a high occurrence of scrub typhus were consistent with the areas experiencing a high distribution of Leptotrombidium scutellare. The incidence of scrub typhus has been rising owing to the increasing population of ticks due to global warming and increased agricultural and outdoor activities. Thus, continuous management of patients and preventive environmental measures are needed to control this disease.