• Korean Journal of Microbiology
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• v.24 no.3
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• pp.251-262
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• 1986

High-molecular-weight ${\beta}-glucosidase$ (EC 3.2.1.21) was purified from the culture filtrate of Trichoderma koningii through a four-step procedure including chromatography on Bio-Gel P-150, DEAE-Sephadex A-50 and SP-Sephadex C-50; and chromatofocusing on Polybuffer exchanger PBE 94. The molecular weight of the enzyme was determined to be about 101,000 by SDS-polyacrylamide gel electrophoreses, and the isoelectric point was estimated to be 4.96 by analytical isoelectric focusing. The temperature optimum for activity was about $55^{\circ}C$, and the pH optimumwas 3.5. The enzyme was considerably thermostable, for no loss of activity was observed when the enzyme was preincubated at $60^{\circ}C$ for 5h. Km values for cellobiose, gentiobiose, sophorose, salicin and $p-nitrophenyl-{\betha}-D-glucoside$ were 99.2, 14.7, 7.09, 3.15 and 0.70 mM, respectively, which indicates that the enzyme has much higher affinity towards $p-nitrophenyl-{\betha}-D-glucoside$ than towards the other substrates, especially cellobiose. Substrate inhibition by $p-nitrophenyl-{\betha}-D-glucoside$ and salicin was observed at the conecntrations exceeding 5mM. Gluconolactone was a powerful inhibitor against the action of the enzyme on $p-nitrophenyl-{\betha}-D-glucoside\;(K_i\;37.9\;{\mu}M)$, wherease glucose was much less effective ($K_i$ 1.95 mM). Inhibition was of the competitive type in each case. Transglucosylation activity was detected shen the readtion products formed from $p-nitrophenyl-{\betha}-D-glucoside$ by the enzyme were analysed using high-performance liquid chromatography.

• International Journal of Concrete Structures and Materials
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• v.10 no.sup3
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• pp.97-108
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• 2016

When the energy performance of concrete is substantially higher than that of normal type concrete, such concrete is regarded as energy efficient concrete (WBSCSD 2009). An experimental study was conducted to investigate mechanical properties of energy efficient concrete with binary, ternary and quaternary admixture at different curing ages. Slump test for workability and air content test were performed on fresh concretes. Compressive strength, splitting tensile strength were made on hardened concrete specimens. The mechanical properties of concrete were compared with predicted values by ACI 363R-84 Code, NZS 3101-95 Code, CSA A23.3-94 Code, CEB-FIP Model, EN 1991, EC 2-02, AIJ Code, JSCE Code, and KCI Code. The use of silica fume increased the compressive strengths, splitting tensile strengths, modulus of elasticities and Poisson's ratios. On the other hand, the compressive strength and splitting tensile strength decreased with increasing fly ash.

• The Korean Journal of Food And Nutrition
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• v.30 no.4
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• pp.771-779
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• 2017

The purpose of this study was to investigate and analyze the total phenolic content (TPC), antioxidant activities (FRAP, ABTS, and DPPH), and antibacterial properties of lotus (Nelumbo nucifera) extracts. Lotus leaves, stems, and seed pods were extracted with deionized water at $95^{\circ}C$, and with 70.5% ethanol at $85^{\circ}C$. The TPC ranged from 8.12 to 215.12 GAE mg/g. The ethanol extract of the seed pod had the highest TPC, and the TPC of the corresponding deionized water extract was 161.45 mg/g. FRAP values ranged from 104.03 to $3,546.39TEAC\;{\mu}mol/g$, ABTS radical cation scavenging activities ranged from 105.11 to $3,956.94TEAC\;{\mu}mol/g$, and DPPH radical scavenging activities ranged from 37.29 to $2,549.46TEAC\;{\mu}mol/g$. $EC_{50}$ values ranged from 0.26 to 9.63 mg/mL, and 0.31 to 21.21 mg/mL for ABTS and DPPH, respectively. The ethanol and deionized water extracts of the seed pod showed higher TPC and stronger antioxidant properties (FRAP, ABTS, and DPPH) than those of characteristic of the leaf extracts. The ethanol and deionized water extracts of the seed pod showed antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa with inhibition zones of 9.0 to 14.0 mm, and the ethanol extract of the leaf showed antimicrobial activity against B. subtilis and S. aureus with inhibition zones of 9.0 and 10.0 mm, respectively. Thus, the lotus seed pod could be used to produce novel teas, and could be a potential source of therapeutic ingredients for food and medicine.

• Korean Journal of Weed Science
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• v.16 no.2
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• pp.108-113
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• 1996

The experiment was conducted to obtain basic information on occurrence ecology system of Aneilema japonica and to establish its effective control systems in dry seeded rice at National Yeongnam Agricultural Experiment Station in 1995. Total amount of weed in dry seeded rice in the following year('95) as affected by control of barnyardgrass at 70 days after seeding(DAS) in previous year('94) was reduced 47.7% at 30 DAS and 7.8% at 60DAS and also exhibited different weed occurrence as compared with no control plot. Especially in control plot at 60 DAS, occurrence of Echinochloa crus-galli was reduced by 46.9%, But the growth of Aneilema japonica which is one of the problems in weed species was drastically increased by 231 % and Persicaria hydropiper by 11.8%, respectively. Suppressed tillering of rice plant as affected by occurring of A. japonica(300-750 plants/$m^2$) occurred from about 40 DAS and rice grain reduced 74-93% compared with hand weeding due to reduced panicle number. Soil applied Butachlor(EC) at 5 DAS, foliar applied Propanil -Butachlor(EC) at 13 DAS and Lgc 40863+Pendimethalin at 20 DAS were very effective for Aneilema japonica. As considering both weed suppression of Aneilema japonica and yield capacity, middle late foliar application of Lgc 40863+Pendimethalin and systematic application of Butachlor at 5 DAS followed by Propanil/Molinate at 25 DAS were the most effective control systems.

• Korean Journal of Remote Sensing
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• v.35 no.6_2
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• pp.1117-1132
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• 2019

Forest covers 30% of the Earth's land area and plays an important role in global carbon flux through its ability to store much greater amounts of carbon than other terrestrial ecosystems. The Gross Primary Production (GPP) represents the productivity of forest ecosystems according to climate change and its effect on the phenology, health, and carbon cycle. In this study, we estimated the daily GPP for a forest ecosystem using remote-sensed data from Moderate Resolution Imaging Spectroradiometer (MODIS) and machine learning algorithms Support Vector Machine (SVM). MODIS products were employed to train the SVM model from 75% to 80% data of the total study period and validated using eddy covariance measurement (EC) data at the six flux tower sites. We also compare the GPP derived from EC and MODIS (MYD17). The MODIS products made use of two data sets: one for Processed MODIS that included calculated by combined products (e.g., Vapor Pressure Deficit), another one for Unprocessed MODIS that used MODIS products without any combined calculation. Statistical analyses, including Pearson correlation coefficient (R), mean squared error (MSE), and root mean square error (RMSE) were used to evaluate the outcomes of the model. In general, the SVM model trained by the Unprocessed MODIS (R = 0.77 - 0.94, p < 0.001) derived from the multi-sites outperformed those trained at a single-site (R = 0.75 - 0.95, p < 0.001). These results show better performance trained by the data including various events and suggest the possibility of using remote-sensed data without complex processes to estimate GPP such as non-stationary ecological processes.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.5
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• pp.602-609
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• 2010

To investigate desalinization patterns of surface reclaimed saline-sodic soil (RSSS) with subsurface layer of macroporous medium, multi-layered soil columns were constructed. For the multi-layered soil columns, gypsum was treated at the rate of 5 cmolc $kg^{-1}$ in surface (top) while coal bottom ash (CBA) was placed into intermediate layer below the gypsum-treated surface soils followed by the reclaimed saline-sodic soil as bottom layer (BL). The lengths of top soil was 30 cm long while the lengths of the CBA were 20 and 30 cm long. The saturated hydraulic conductivities (Ksat) were $0.39{\times}10^{-4}$ and $0.31{\times}10^{-4}cm\;sec^{-1}$ for RSSS(30 cm)-CBA(20 cm)-BL(20 cm) and RSSS(30 cm)-CBA(20 cm)-RSSS(20 cm), respectively while the lowest $K_{sat}$. was $0.064{\times}10^{-4}cm\;sec^{-1}$ for RSSS(30 cm)-CBA(20 cm)+BL(20 cm). The time required to reach the lowest EC in eluent, 0.3 dS $m^{-1}$ from 33.9 dS $m^{-1}$ was shorter in multi-layered soil columns with GR-CBA than that of RS-SRS, representing that rate of desalinization was greater than 99%. Exchangeable Na decreased by 94.8~96.2 %, while exchangeable Ca increased by 98~129 %.

• Journal of agriculture & life science
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• v.44 no.5
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• pp.91-99
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• 2010

The objective of this work was to investigate the influence of ultrasonification on extraction yield and chemical properties of green tea infusion. Changes in total soluble matter(TSM), vitamin C, total phenolic compounds, flavonols, catechins, caffeine, free amino acids contents in green tea infusion(GTI) influenced by ultrasonification at $60^{\circ}C$ of extraction temperature for 1, 5, 30, and 60 min were investigated. The amount of infused TSM increased about 5.3% by ultrasonification for 60min. Vitamin C contents also increased 0.21, 0.16, 0.31 mg/g from 1 to 30 min by ultrasonification. However, vitamin C decreased from 2.47 to 2.22 mg/g at 60min. Total phenol compounds contents increased about 10~13 mg/g on all extraction times by ultrasonification. Flavonols such as, myricetin, quercetin, kaempferol were increased to doubled contents as an influence of ultrasonification. Catechins such as, EGCG, EGC, ECG, EC, (+)-C and caffeine contents showed same tendency as the results of vitamin C. On the other hand, result of free amino acids showed different tendency. All amounts of free amino acids did not increase by ultrasonification. Consequently, content of bioactive compounds such as, vitamin C, total phenolic, flavonols and catechins in green tea infusion were influenced by ultrasonification.