• Journal of the Korean Chemical Society
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• v.64 no.1
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• pp.30-37
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• 2020

The purpose of this study was to identify the problems faced by students in sub-microscopic representation of acid-base reactions. Herein, we selected 30 students of 12^th grade science classes, who had studied various acid-base models. In order to investigate the sub-microscopic representation ability of the students, we developed nine items related to various contexts, such as one type of solute and solvent, two types of solutes and solvent, cases with water as solvent or with nonaqueous solvents. For all items, we consistently observed lack of concept of chemical change. In context of aqueous and nonaqueous solutions, the frequency of lack of concept of chemical bonding was high if ammonia was the solute or solvent. Moreover, the frequency of lack of concept related to the degree of electrolytic dissociation was high. Therefore, chemistry teachers should understand that students' ability to sub-microscopic representation of acid-base reactions can be enhanced by analyzing the difficulties faced by the students in solving diverse acid-base problems.

• Journal of Korean Society on Water Environment
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• v.34 no.1
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• pp.109-120
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• 2018

Sidestream in domestic wastewater treatment plants contains high concentration of ammonium, which increases nitrogen loading rate in the mainstream. The process for deammonification consisting of partial nitritation-anaerobic ammonium oxidation (ANAMMOX) and heterotrophic denitrification is an economical method of solving this problem. Currently, about 130 full-scale deammonification plants are fully operating around the world, but none is in Korea. In order to transfer the principal information about sidestream deammonification processes to researchers and operators, we summarized basic concepts, processes type, and key influence factors (e.g., concentration of nitrogen compounds, dissolved oxygen (DO), temperature, and pH). This review emphasis on the processes of single-stage sequencing batch reactor (SBR) deammonification, which are widely used as full-scale plants. Since simultaneous processes of partial nitritation, ANAMMOX and heterotrophic denitrification occur in a single reactor, the single-stage SBR deammonification requires appropriate control/monitoring strategies for several operating factors (DO and pH mostly) to achieve efficient and stable operation. In future, AB-process consisting of A-stage (energy harvesting from organics) and B-stage (ammonium removal without organics) will be applied to the wastewater treatment process. Thus, we suggest mainstream deammonification for B-stage connected with the sidestream deammonification as seeding source of ANAMMOX. We expect that many researchers will become more interested in the sidestream deammonification.

• Journal of Korean Society on Water Environment
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• v.23 no.3
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• pp.397-402
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• 2007

Anammox reactor seeded with sewage sludge from RBC reactor and anaerobic granule from full-scale UASB reactor treating distillery wastewater was operated. Mixed granule and suspended sludge in the ammonium oxidizing process were taken and analyzed to investigate microbial community structure by molecular methods such as gene cloning and phylogenetic tree analysis after 250 days of continuous cultivation. The average nitrogen removal rate showed $0.9kg\;N/m^3-day$ after 250 days of continuous operation, then the maximum nitrogen removal rate showd $1.9kg\;N/m^3-day$ when $2.1kg\;N/m^3-day$ of nitrogen loading rate was applied. As results of gene cloning and phylogenetic tree analysis, Three kinds of phylum were found to be Proteobacteria, Acidobacteria and Planctomycetes (anammox bacteria) in mixed granule. Five kinds of phylum were found to be Proteobacteria, Chlorobi, Planctomycetes, Nitrospirae and Verrucomicrobia in suspended sludge. We found planctomycete KSU-1 and putative new anammox bacteria in the reactor. Microbial structure represented different consortia depending on the types of sludge in the anammox reactor.

• Journal of Korean Society on Water Environment
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• v.36 no.3
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• pp.220-228
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• 2020

Anaerobic ammonium oxidation (AMX) is a cost-efficient biological nitrogen removal process. The coexistence of ammonia-oxidizing bacteria (AOB) in an AMX reactor is an interesting research topic as a nitrogen-related bacterial consortium. In this study, a sequencing batch reactor for AMX (AMX-SBR) was operated with a conventional activated sludge. The AOB in an AMX bioreactor were identified and quantified using terminal restriction fragment length polymorphism (T-RFLP) and real-time qPCR. A T-RFLP assay based on the ammonia monooxygenase subunit A (amoA) gene sequences showed the presence of Nitrosomonas europaea-like AOB in the AMX-SBR. A phylogenetic tree based on the sequenced amoA gene showed that AOB were affiliated with the Nitrosomonas europaea/mobilis cluster. Throughout the enrichment period, the AOB population was stable with predominant Nitrosomonas europaea-like AOB. Two OTUs of amoA_SBR_JJY_20 (FJ577843) and amoA_SBR_JJY_9 (FJ577849) are similar to the clones from AMX-related environments. Real-time qPCR was used to quantify AOB populations over time. Interestingly, the exponential growth of AOB populations was observed during the substrate inhibition of the AMX bacteria. The specific growth rate of AOB under anaerobic conditions was only 0.111 d^-1. The growth property of Nitrosomonas europaea-like AOB may provide fundamental information about the metabolic relationship between the AMX bacteria and AOB.

• Ocean and Polar Research
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• v.26 no.4
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• pp.635-646
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• 2004

Chronic outbreaks of green tide in the Nakdong estuary toll a heavy socioeconomic cost. The paper investigates the influence of sediments on the nitrogen eutrophication, being claimed as the primary cause of green tide. To measure the flux of nitrate at the sediments-water interface, sediment cores were taken in Jan., Mar., May and Sep., 2000 at Noksan located in the West-Nakdong river estuary. The dissolved oxygen was profiled and then the pore water was extracted in situ. Core samples were analyzed for their textural characteristics. Cores were incubated by a novel technique to measure the fluxes of nitrate $(NO_3^-)$ and ammonia $(NH_4^+)$ at the sediment-water interface. The dissolved oxygen was depleted usually within several millimeters in the top sediments. Nitrate started to decrease drastically at the layer where dissolved oxygen was nearly depleted. Nitrate was also exhausted within several centimeters, followed by ammonia build up rapidly. The flux at the sediments-water interface calculated from the pore water concentrations revealed that nitrate was removed from the water column into the sediments. The sediment incubation experiment confirmed the above result. On the other hand ammonia were released from the sediment to the water column. As the incubation went on, however, the nitrate concentration in the overlying water was dropped below that of a top sediment. Then the flux is reversed, i.e., nitrate was released from the sediments to the water column. The implication is that the sediment can supply nitrate to the water column if it falls below a certain level. Thus it is likely that sediments in the eutrophicated river buffers the nitrate concentration in the water column, which leads to a prolonged green tide.

• Analytical Science and Technology
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• v.5 no.4
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• pp.425-431
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• 1992

The concentration and determination of trace phosphate ion was studied by $La(OH)_3$ coprecipitaiton. Phosphate ions in 1.0L samples were coprecipitated with lanthanium hydroxide at pH 9.5 adjusted with ammonia solution. The precipitates were floated with the aid of mixed surfactant(1:8 sodium oleate/sodium dodecyl sulfate) and nitrogen gas bubbles. The floated precipitate was collected in suction flask from the solution. The precipitate were washed with dil. ammonia solution and dissolved in sulfuric acid. The phosphate ion in the concentrated solution was finally determinated by UV/VIS spectrophotometry using the molybdenium blue method. The proposed method could be applied to the determination of phosphate ion in tap water and river water.

• Journal of Korean Society on Water Environment
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• v.24 no.3
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• pp.311-316
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• 2008

This study aimed to obtain equilibrium concentration on adsorption removal of ammonia nitrogen by activated carbon, to express the adsorption characteristics following Freundlich isotherm and also, based on the value obtained, to investigate the relationship between physical characteristics of activated carbon and dynamics of ammonia nitrogen removal by obtaining rate constant and effective pore diffusivity. The results summarized from this study are as follows. It was noted that powdered activated carbon showed better adsorption ability than granular activated carbon. The value of constant (f) of Freundlich isotherm of powered activated carbon was $4.6{\times}10^{-8}$ which is bigger than that of granular activated carbon. The adsorption rate constant on ammonia nitrogen of powered activated carbon with high porosity and low effective diameter was highest as 0.416 hr-1 and the effective pore diffusivity ($D_e$) was lowest as $1.17{\times}10^{-6}cm^2/hr$, and the value of ammonia nitrogen adsorption rate constant of granular activated carbon was $0.149{\sim}0.195hr^{-1}$. It was revealed that, with the same amount of dosage, the adsorptive power of activated carbon with lower effective diameter and bigger porosity was better and its rate constant was also high. With a little adsorbent dosage of 2 g, there was no difference removal ability of ammonia nitrogen as change of adsorption properties.

• Journal of Korean Society on Water Environment
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• v.23 no.5
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• pp.600-606
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• 2007

Livestock wastewater containing concentrated organic matters and nutrients has been known as one of the major pollutants. It is difficult to apply the conventional activated sludge process to treat livestock wastewater because of high Non-biodegradable (NBD) matter and ammonia. The objectives of this study are to remove NBD matters including aromatic compounds and ammonia in livestock wastewater using Coagulation-Fenton oxidation-Zeolite (CFZ) processes and ascertain applicable feasibility in the field through pilot plant experiment. NBD matters and color remained in the treated water were removed over 92% by Fenton oxidation as the second treatment process. Ammonia was removed by over 99.5% in the zeolite ion exchange process as the last treatment method. From $UV_{254}$, $E_2/E_3$ ratio and GC/MS analyses of treated water at each process, the aromatic compound was converted to aliphatic and aromaticity was decreased. In pilot scale test, organics and ammonia removal efficiencies were not much different from the result of lab-scale test at various operation conditions. Furthermore, reaction time and dosage of Fenton reagent in pilot scale experiment reduced by 40 min and 50% rather than in lab-scale test. $BOD_5$, $COD_{Mn}$, SS, T-N and T-P of treated water in the pilot-scale experiment also met the effluent standards.

• Journal of Korean Society on Water Environment
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• v.18 no.4
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• pp.355-363
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• 2002

A demonstration plant was carried out to investigate the removal efficiency of $NH_3-N$ and $KMnO_4$ consumption depending on the existence of pre-chlorination for the ozonation and activated carbon process in the S water treatment plant which is located at the middle of Keum River. The averge removal efficiency of $KMnO_4$ consumption for $O_3/GAC$ processes with pre-chlorination and $O_3/BAC$ processes without pre-chlorination were 48.6% and 50% respectively. It is similar to removal effect of $KMnO_4$ consumption for GAC and BAC process depending on the existence of pre-chlorination. Otherwise, the removal of THMFP for GAC and BAC process was 58% and 68% respectively. $NH_3-N$ was not almost removed by sand filter and ozonation, but the average removal efficiency in the BAC process was about 31%. Especially, $NH_3-N$ was not almost removed by $O_3/BAC$ processes at the low temperature (below $$10^{\circ}C$$) in the winter season, $O_3/BAC$ processes have the advantage of removal of organic substance when it is compared to pre -chlorination followed by $O_3/GAC$ processes. Pre-chlorination followed by $O_3/GAC$ processes were required to remove $NH_3-N$ in the winter season because the removal of $NH_3-N$ was almost ineffective by $O_3/BAC$ process.

• Journal of the Korean Society of Food Science and Nutrition
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• v.13 no.4
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• pp.437-443
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• 1984

Muscular exercise induced by electrical stimulation of femoral nerves in perfused rat hindquarters(5 contractions per sec) in the presence of insulin and glucose effected a rapid increase(c. a. two-fold) in the level of citric acid cycle intermediates. The highest values were found within one minute of stimulation. The tissue concent ratios of lactate, pyruvate and alanine increased rapidly on initiation of exercese. Release of lactate also increased rapidly, whereas that of pyruvate was only moderately elevated. In the course of three minute exercese, the sum of alanine, glutamate and aspartate was only transiently elevated. A fall in creatine-p and ATP in the stimulated muscle was accompanied by increases in tissue level of AMP and release of ammonia into perfusing medium. However, the changes in glutamine were small. It is concluded that the pool of citric acid cycle intermediates is expanded during muscular work due (a) to an elevated level of pyruvate, leading to shifts in the levels of alanine and cycle intermediates vie trans-amination reactions and (b) to stimulation of the purine nucleotide cycle due to elevated AMP, resulting in generation of cycle intermediates and ammonia at the expense of aspartate.