• Title/Summary/Keyword: 알코올탈수소효소

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The Roles of Lipid Supplements in Ethanol Production Using a Continuous Immobilized and Suspended Cell Bioreactor (연속식 고정화 및 현탁 세포 생물 반응기에 의한 에탄을 생성중 지질 첨가 영향)

  • Gil, Gwang-Hoon
    • Applied Biological Chemistry
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    • v.39 no.1
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    • pp.1-8
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    • 1996
  • A one-stage, continuous-flow bioreactor with both immobilized and suspended cells was used to investigate the roles of lipid supplements in ethanol production by Saccharomyces cerevisiae. The reactor performance and the level of alcohol dehydrogenase(ADH) activities of the suspended cells, grown under various conditions, were measured. When ergosterol and/or oleic acid were added with surfactants to the yeast culture grown under non-aerated conditions, remarkable increases in ethanol production and cell growth was achieved, but specific ADH activities were not affected. Especially, no difference of specific ADH activities of the suspended cells grown under aerated and non-aerated condition was observed. The addition of the surfactant as a supplement also resulted in significant increases in ethanol production, cell growth, and specific ADH activity. When ergosterol and oleic acid were added to the yeast culture exposed to higher ethanol concentration($>40\;g/{\ell}$) level, ethanol production, cell growth, and specific ADH activity were increased, but the addition of surfactant was as effective as at lower ethanol concentration level. The results indicated that lipid supplements were more effective at higher ethanol concentration level than at lower ethanol concentration level during ethanol production. ADH isozyme patterns of the yeast cultures grown under various conditions on starch gel electrophoresis showed only one major band, probably ADH I. The migrating distance of the major isozyme, however, varied slightly according to the culture conditions of the cells. No apparent correlation was found between specific ADH activity and amount of ethanol produced. Cell mass was more important factor for ethanol production than specific ADH activity of the cells.

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Characterization of Alcohol Dehydrogenase Encoded by Zymomonas mobilis Gene Cloned in Escherichia coli (Escherichia coli 형질전환체가 생산하는 Zymomonas mobilis 알콜 탈수소 효소의 분석)

  • 신병식;윤기홍;박무영
    • Microbiology and Biotechnology Letters
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    • v.18 no.3
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    • pp.268-272
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    • 1990
  • The structural gene (zadhII) encoding an alcohol dehydrogenase II from Zyrnornonas mobilis was cloned into Escherichia coli in our laboratory (Yoon et al., 1989. Kor. J. Microbiol. Biotechnol.). From E. coli (pADS93) carrying the zadhII gene, the Z mobilis alcochol dehydrogenase II (ZADH-II) was purified by sonication, $(NH_4)_2SO_4$, fractionation, and chromatography. The ZADH-I1 enzyme produced by Z. mobilis cell was also purified to compare to the enzyme produced by E. coli (pADS93). The purified enzyme from cell extract of E. coli (pADS93) was identified to be a tetramer being composed of four identical subunits having molecular weight of 40, 000 dalton like that of Z. mobilis. The pH optimum for the reaction oxidizing ethanol to acetaldehyde was 10.0 while the optimum for the reverse reaction was 7.5-8.5. The apparent $K_m$ values for ethanol and NAD + were $1.2 \times 10^{-1}M$and $5.1\times 10^{-5}M$, respectively. In addition, it was found that the $K_m$ value for acetaldehyde was very lower than that for ethanol.

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Enhanced Activities of Alcohol Methabolism by Extracts from Hericium erinaceum Hypha Cultivated with Artermisia capillaris(HEAC) (인진쑥 배지에서 배양한 노루궁뎅이버섯 균사체 추출물(HEAC)의 알코올 대사촉진 활성)

  • Choi, Won-Sik;Jang, Do-Yoen;Cha, Kyung-Min;Park, Chun-Kyu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.1
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    • pp.189-194
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    • 2008
  • Alcohol concentration in the blood was effectively decreased by extracts from Hericium erinaceum hypha cultivated with Artermisia capillaris medium(HEAC), Hericium erinaceum hypha and Artermisia capillaris after dirnking. Also, the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the blood was studied. As a result of testing an alcohol concentration in the blood, the alcohol in the blood was not detected after 170 min, in case of HEAC and after 210 min, in case of Hericium erinaceum. Compared to control, each activities of alcohol dehydrogenase of HEAC and Hericium erinaceum hypha was showed up to 154% and 148% respectively. The activities of the acetaldehyde dehydrogenase of both extracts from HEAC and Hericium erinaceum was maintained in the range of 104 to 110% compared to control. In conclusion, such extracts represent significant effect to facilitate decomposition of alcohol.

Effect of Plant Extracts on the Activity of Alcohol Dehydrogenase and the Antioxidation in Alcohol-treated Rat Hepatocyte (알코올 투여한 흰쥐 간세포내 알코올 탈수소효소의 활성과 항산화에 미치는 식물추출물들의 영향)

  • 조성환;김지철;김성완
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.4
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    • pp.679-683
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    • 2001
  • This study was purposed to compare the activity of alcohol dehydrogenase and antioxidative effects of several plant extracts in the alcohol-treated rat liver. Sprague-Dawley rat weighing about 200 g were divided into the following 6 groups : normal, alcohol group and 4 different plant extracts administrated groups(Soybean sprout, Pine needle, Lentinus edodes, acanthopanacis cortex). Each plant extract was administrated orally by 200mg/kg b.w./day for 8 days before the alcohol treatment (5 g of 30% alcohol /kg b.w. by i.p.injection). All rats were sacrificed at 90 min after the alcohol treatment. The alcohol concentrations in serum of Soybean sprout and pine needle group were significantly lower than the Lentinus edodes and Acanthopanacis cortex group. The activity of alcohol dehydrogenase in the hepatic cytosol of Soybean sprout and Pine needle group was also significantly higher than the alcohol and the other groups However, the activity of catalase seemed not to be affected, although the extract groups showed slightly higher activities of catalase than the alcohol group. These results may indicate that the extracts of Soybean sprout and Pine needle were relatvely effective on the alcohol degradation. the activity of blutathione-peroxidase and lipid peroxidaton of all of the extract groups were significantly lower than the activity of alcohol group. These results can suggest that all of the use plant extracts more or less have an antioxidative effect on the alcohol-induced oxidation and especially, extracts of Soybean sprout and Pine needle have an stimulating effect on the alcohol absorption and degradation.

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Natural herbal extract complex induces the degradation of alcohol and acetaldehyde and reduces the breath alcohol concentration (천연소재복합물의 알코올 및 아세트알데히드 분해활성 및 호기중 알코올 농도 감소)

  • Hwang, Ji Hong;Kim, Mi-Yeon
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.3
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    • pp.381-392
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    • 2020
  • This study is purposed to check up the natural 12 kinds of herbal extracts suitable for hangover and based on the results of contents of phenolic compounds, ABTS radical scavenging activity, alcohol dehydrogenase (ADH), and acetaldehyde dehydrogenase (ALDH). Selected 8 kinds of herbal extracts are blended according to the efficacy and the pearson's correlation between each content and activity. C. sinensis var. sinensis, P. densiflora Gnarl and P. lobata Ohwi showed excellent ADH activity, P. lobata Ohwi had a strong correlation between the content and efficacy, and C. sinensis var. sinensis, P. densiflora Gnarl had a negative correlation. Through the ADH and ALDH activity test of F.1 to F.7, the F.7 showed the highest synergic effect and selected as an optimal formulation. F.7 intake-group, the breath alcohol concentration was significantly reduced to 58% after 30 minutes and 27% after 120 minutes, compared to right after alcohol consumption. After alcohol consumption, there was a significant improvement effect (p<0.05) in tired and thirst in the intake group compared to the non-intake group.

Cloning and Expression of the Structural Gene for Alcohol Dehydrogenase of Zymomonas mobilis in Escherichia coli (Zymomonas mobilis 알코올 탈수소 효소 유전자의 Cloning과 Escherichia coli 에서의 발현)

  • Yoon, Ki-Hong;Shin, Byung-Sik;M.Y Pack
    • Microbiology and Biotechnology Letters
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    • v.17 no.4
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    • pp.301-306
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    • 1989
  • A genomic library of Zymomonas mobilis DNA was constructed in Escherichia coli using plasmid pUC9 Allyl alcohol was used to screen a genomic clone expressing alcohol dehydrogenase. The plasmids isolated from two clones, which were sensitive to allyl alcohol, were found to be related and to share a common 2.6 kb fragment encoding alcohol dehydrogenase II identified as one of two isozymes in Z. mobilis by staining for alcohol dehydrogenase activity on polyacrylamide gel and spectrophotometric analysis of several substrate oxidations.

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Activities of Antioxidation and Alcohol Dehydrogenase Inhibition of Methanol Extracts from Some Medicinal Herbs (약용식물 추출물에 대한 항산화성과 알코올 탈수소효소 저해성 연구)

  • 문지숙;김선재;박윤미;황인식;김의형;박정욱;박인배;김상욱;강성국
    • Food Science and Preservation
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    • v.11 no.2
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    • pp.201-206
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    • 2004
  • The activities of antioxidation and alcohol dehydrogenase in hibitionin methanol extracts of thirty two medical herbs were tested using the method of DPPH activity, nitrite scavenging effect and alcohol dehydrogenase assay in vitro. In DPPH method, Eugenia caryophyllata, Thea sinensis, Paeonia suffruticosa, Alnus japonica showed over 90 % of free radical scavenging activities. The nitrite scavenging ability appeared Zanthoxylum bungeanum, Alnus japonica, Thea sinensis, Hovenia dulcis(cortex) and Illicium verum showed the high value. In connection with in vivo alcohol metabolism, thirteen medicinal herbs were screened for inhibition. As a reasult, we found significant inhibition of ADH by methanolic extracts of Glycyrrhiza uralensis, Pueraria thunbergiana(radix), Alnus japonica. These results indicate that the antioxidative effect was strongly related with alcohol dehydrogenase inhibitor; Thea sinensis and Alnus japonica.

Immunological Characterization and Localization of the Alcohol-dehydrogenase in Streptococcus pneumoniae (폐렴구균 알코올탈수소효소의 세포 특이성 및 세포내 분포)

  • 권혁영;박연진;표석능;이동권
    • Korean Journal of Microbiology
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    • v.37 no.3
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    • pp.221-227
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    • 2001
  • Heat shock proteins serve as chaperone by preventing the aggregation of denatured proteins and promote survival of pathogens in harsh environments. In bacteria, ethanol shock induced the major chaperone GroEL and DnaK, but in Streptococcus pneumoniae, it induced neither GroEL nor DnaK but alcohol dehydrogenase (ADH). In this study, ADH gene encoding a 104-kDa (p104) protein was identified and characterized. The deduced amino acid sequence of pneumococcal ADH shows homology with other members of the ADH family, and particularly with Entamoeba histolytica ADH2 and E. coli ADH. S. pneumoniae adh is composed of 883 amino acids and its estimated isoelectric point is 6.09. Although ADH is conserved between S. pneumoniae and E. coli, immunoblot analysis employing antisera raised against pneumococcus ADH demonstrated no cross-reactivity with ADH analog in Eschericha coli, Staphylococcus aureus and human HeLa cells. Also secretion of ADH was demonstrated by subcellular fractionation and immunoblot analysis of proteins. These results suggest that S. pneumoniae ADH could be a highly feasible candidate for both diagnostic marker and vaccine.

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Effects of Paecilomyces tenuipes Water Extract on the Alcohol Metabolism of Rats (눈꽃동충하초 물추출물이 흰쥐의 알코올대사에 미치는 영향)

  • Kim, Ji-Myung;Park, Jae-Hwan;Kim, Mi-Kyung;Chun, Hyang-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.3
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    • pp.396-400
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    • 2008
  • The effect of Paecilomyces tenuipes water extract (PTWE) on the alcohol metabolism was examined on rats. PTWE of 0, 30, 100 mg/kg body weight was administrated orally to the rats 30 min before oral treatment of 3 g/kg body weight of alcohol. Blood alcohol and acetaldehyde concentrations were measured 0.5, 1, 3, 6, 9 hr after alcohol treatment. Hepatic alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ADH), microsomal ethanol oxidizing system (MEOS) activities were measured 9 hr after alcohol treatment. There were no differences in blood alcohol concentrations and area under the curve (AUC) of alcohol. PTWE decreased acetaldehyde concentration and there were significant differences after 6 hr in 30 mg/kg PTWE and after 3 and 9 hr in 100 mg/kg PTWE, respectively. In particular, 100 mg/kg PTWE decreased AUC of acetaldehyde by 44%. However, there were no changes in the hepatic ADH, ALDH, and MEOS activities.

Preparation and Evaluation of Physiological Activity of White Bread Containing Korean Blue Mussel (국산 양식 홍합 함유 식빵의 제조 및 생리활성 평가)

  • Jo, Min-Ji;Kim, Yun-Ah;Lee, Seung-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.11
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    • pp.1623-1629
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    • 2016
  • White breads containing Korean blue mussel (Mytilus edulis) powder were prepared and characterized. WB (white bread without blue mussel) and four different MBs (white breads containing blue mussel; number in front of MB means added % of blue mussel powder per wheat flour) were prepared by the straight dough method. With addition of blue mussel to bread, lightness decreased, whereas redness and yellowness increased. Addition of blue mussel did not significantly affect specific volumes of breads. DPPH and ABTS radical scavenging activities significantly increased with increasing blue mussel content. Addition of blue mussel to breads also increased alcohol dehydrogenase and acetaldehyde dehydrogenase activities. In the sensory test, 1MB acquired relatively high points for taste, flavor, texture, and preference. The results indicate that blue mussel can be applied to white bread to improve physiological functions without reduction of physicochemical characteristics.