• Title/Summary/Keyword: 알카리분해

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Characteristics of Whey Protein (WPC-30) Hydrolysate from Cheese Whey (치즈유청으로부터 제조한 유청단백질 가수분해물의 특성에 관한 연구)

  • Yoon, Yoh-Chang;An, Sung-Il;Jeong, A-Ram;Han, Song-Ee;Kim, Myeong-Hee;Lee, Chang-Kwon
    • Journal of Animal Science and Technology
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    • v.52 no.5
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    • pp.435-440
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    • 2010
  • Whey protein concentrate (WPC) is widely used to increase the nutritional and functional properties of food. In this study, the physiochemical and functionality of WPC-30 hydrolysates were examined to evaluate the possibility of application in the food industry. The WPC-30 was manufactured using ultrafiltration and spray-drying, and then hydrolyzed with proteolytic enzyme including alcalase, flavourzyme, nuetrase and protamex. Enzymatic hydrolysis had a significant influence on the physicochemical properties as evident from the increased foaming capacity, solubility. Alcalase caused highest protein hydrolysis (3.26%) and the bitterness. Foaming capacity was largest in WPC-30 hydrolysate treated with flavourzyme. Protein solubility at various levels of pH was highest in protamex-treated WPC-30 hydrolysate. However, the solubility of WPC-30 hydrolysates was significantly improved in alkaline condition than in acidic and neutral conditions. The study revealed that spray dried enzyme modified WPC can be used in various functional food.

Studies on the Microbial Utilization of Agricultural Wastes (Part 3) Effects of Alkali Treatments of the Wastes on the Production of Cellulosic Single-Cell Protein (농산폐자원의 미생물학적 이용이 관한 연구(제3보) -알카리 전처리가 -섬유소단세포단백 생산에 미치는 영향-)

  • Bae, Moo;Kim, Byung-Hong
    • Microbiology and Biotechnology Letters
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    • v.2 no.2
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    • pp.79-82
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    • 1974
  • Present experiments were designed to estimate the effects of pretreatments by various kinds of alkalis to the agricultural wastes such as cereal straws as the substrate on the production of cellulosic single-cell protein. Among the various kinds of alkalis NaOH was proved to be the most effective on improving the digestibility of cellulose by the bacteria isolated. NH$_4$OH which is inferior to NaOH in the effectiveness of treatment might have more economic advantage in the price, and the ammonium salt resulted from the neutralization can be used as the nitrogen source by bacteria. The treatment with higher concentration than 1 normality of NH$_4$OH didn't increase the productivility of cell mass. About five per cent of (NH$_4$)$_2$SO$_4$ in medium resulted from the neutralization didn't have any influence in the cell mass productivility. Futhermore, the cell mass productibility was higher in the case of neutralization than alkali free washing. The digestibility of straws was increased from 7.9% to 46.0% by NH$_4$OH treatment, and 6.3∼6.45g of dry cell were obtained from 40g of NH$_4$OH treated straws. In the case of NaOH treatment, 8.6g of cell mass was obtained from 40g of substrate.

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Biocompatibility of Ti-8Ta-3Nb alloy with fetal rat calvarial cells (백서 태자 두개관세포에서 Ti-8Ta-3Nb 합금의 생체적합성)

  • Cho, In-Goo;Cui, De-Zhe;Kim, Young-Joon;Lee, Kyung-Ku;Lee, Doh-Jae
    • Journal of Periodontal and Implant Science
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    • v.36 no.4
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    • pp.849-861
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    • 2006
  • 타이타늄은 기계적 특성이 우수하고 생체적합성이 뛰어나 의료용 장비의 주 재료로 사용되고 있으며 타이타늄 보다 기계적 특성이 더 우수한 타이타늄 합금들(주로 Ti-6Al-4V와 Ti-6Al-7Nb 합금)도 개발되어 치과와 의료용 임플란트로 사용되고 있다. 그러나 타이타늄 합금 성분들 중 알루미늄 (aluminum)과 바나디움(vanadium)은 인체에 노출되면 세포손상과 신경계에 문제를 일으킬 수 있다. 따라서 인체에 독성이 없으면서 기계적 성질과 생체적합성이 우수한 타이타늄 합금의 개발이 필요하다. 최근 인체에 독성이 없는 성분들이 함유된 새로운 ${\beta}$ - 형태의 타이타늄 합금들이 개발되고 있는데, ${\beta}$ - 타이타늄 합금은 그 기계적 성질이 기존의 ${\alpha}+{\beta}$ 타이타늄 합금에 비해 우수하다고 알려져 있다. 최근 새로운 ${\beta}$ - 타이타늄 합금이 전남대학교 부설 타이타늄 연구소에서 개발되었다. 이 연구는 새로 개발된 ${\beta}$ - 타이타늄 합금의 생채 적합성을 세포 증식도, 알카리 인산 분해 효소 활성과 유전자 증폭을 통해 알아보고자 하였다. 그 결과는 다음과 같다; 1. Titanium-6aluminum-4vanadium (Ti-6Al-4V) 합금 표면애서의 세포 증식율은 Titanium-Titanium8Tantalum-3Niobium (Ti-8Ta-3Nb) 합금과 순수 타이타늄 표면에 비해 유의하게 낮았다(p<0.00l). Ti-8Ta-3Nb 합금 표면에서의 증식도는 순수 타이타늄 표면과 유사하였다. 2. Ti-8Ta-3Nb 합금과 순수 타이타늄에서 배양된 세포이 알카리 인산 분해 효소의 활성도는 Ti-6Al-4V 합금에서의 것보다 유의하게 높았다 (p<0.001). 3. 유전자 증폭 분석 결과, Ti-8Ta-3Nb 합금과 순수 타이타늄에서 collagen type I과 bone sialoprotein mRNA 가 유사한 수준으로 발현되었다. 이상의 결과는 생체 적합성 측면에서 Ti-8Ta-3Nb 합금과 순수 타이타늄의 차이가 없음을 보여주며 따라서 Ti-8Ta-3Nb 합금이 의학 및 치의학 영역에서 새로운 임프란트 재료로 사용될 수 있음을 의미한다.

Agricultural application of natural polymers chitin and chitosan (천연고분자 키틴·키토산의 농업적 활용)

  • Jung, Woo-Jin
    • Food Science and Industry
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    • v.53 no.1
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    • pp.33-42
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    • 2020
  • In accordance with the recent trend of environmentally friendly agricultural policy, product registration of agricultural chitosan among the organic materials has been displayed in various forms such as soil improving agent, crop growth, and pest control. Chitin production industry is expected to bring competitiveness by producing low-quality and low-cost chitin for agriculture, rather than high-quality and high-cost for food, medical products. Since there are various soil microorganisms that can decompose chitin and chitosan in farm soil where crops are produced, it can be applied usefully to agricultural sites suitably for crop growth and pest control using chitin and chitosan as substrates. The purpose of this study is to compare and analyze the registration status of organic materials companies using chitin and chitosan raw materials in the organic materials information system of the NAQS, and to provide an opportunity to further expand the agricultural use of domestic chitin and chitosan.

Chemical Characteristics of Timbers from the Yeongheungdo Shipwreck (영흥도선의 화학적 특성)

  • Cha, Mi Young
    • Journal of Conservation Science
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    • v.33 no.1
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    • pp.35-42
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    • 2017
  • The Yeongheungdo shipwreck was excavated in the sea of Seomeopbeol, Ongjin-gun, Incheon, Korea. Chemical characterization of timbers from the shipwreck occurred via maximum moisture content, chemical composition, FT-IR, solid state $^{13}C$ NMR, and XRD analyses. As maximum moisture content of the outer part of the timbers averaged 623%, a phenomenon in that lignin increased relatively, according to the decrease in cellulose, and the ash content increased, which is typical of waterlogged archaeological wood was shown. The results from the FT-IR and solid state $^{13}C$ NMR analyses showed that the cellulose band of the outer part had disappeared or had remarkably decreased. In addition, the bands assigned to lignin dramatically increased. However, regarding the inner part of the timbers, hemicellulose and some crystalline cellulose and amorphous cellulose bands decreased. Therefore, a large difference exists between the degradation levels of the outer and inner parts of the timbers of the Yeongheungdo shipwreck.

A New Method of Colorimetric Determination of Piperine Using p-Nitrophenyl Diazonium Fluoborate in Pepper (Piper Nigrum L.) (p-Nitropheneyl Diazonium Fluoborate를 이용한 후추 Piperine의 새로운 비색정량법)

  • Rhee, Seong-Kap
    • Korean Journal of Food Science and Technology
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    • v.6 no.2
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    • pp.56-60
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    • 1974
  • A survey of the available methods for the determination of piperine was made, and a new method, developed based upon alkaline hydrolysis at about $140^{\circ}C$ followed by colorimetric determination of the liberated piperidine. Piperidine on treatment with p-nitrophenyl diazonium fluoborate reagent gives a red colored complex which has an adsorption maximum at 530nm. The method measures the total pungency in pepper and applicaple to piperine of black pepper and oleoresin of black pepper. The advantage of the present method is that the pungent compounds can be determined in micro samples.

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Studies on Distribution of the Mononucleotides in Ganoderma lucidum (영지(靈芝)의 모노뉴크레오티드 성분의 분포에 관한 연구)

  • Kim, Jong-Hyup;Nam, Jeong-Sook
    • The Korean Journal of Mycology
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    • v.12 no.3
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    • pp.111-116
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    • 1984
  • Ribonucleic acid contents and mononucleotides distribution from the mycelium and fruit bodies of Ganoderma lucidum were studied. P.E.I. cellulose TLC and HPLC were applied in this study. The obtained results are as follows; The levels of ribonucleic acids from the young basidiocarp mycelium were higher than those of mature basidiocarp. Guanosine 5'-monophosphate and xanthosine 5'-monophosphate were found in both young basidiocarp mycelium and mature basidiocarp. The levels of guanosine 5'-monophosphate and xanthosine 5'-monophosphate from the young basidiocarp were higher than those of the mature basidiocarp. However, inosine 5'-mono­phosphate was not detected.

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Studies on Photosentitive Polymers (XI). Studies on Photodegradative Reaction of Naphthoquinone-1,2-diazide-5-sulfonyl Esters (感光性 樹脂에 關한 硏究 (第11報). Naphthoquinone-1,2-diazide-5-sulfonyl Esters의 光分解反應)

  • Shim Jyong Sup;Kang Doo Whan
    • Journal of the Korean Chemical Society
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    • v.19 no.4
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    • pp.280-285
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    • 1975
  • The degradation mechanisms of naphthoquinone-1,2-diazide-5-sulfonyl esters(PGND, BEND and PVAND) of polyglyceryl phthalate (PG), bisphenol A-epichlorohydrin (BE) and polyvinyl alcohol (PVA) were studied by infrared absorption spectra. Absorption band due to C=N=and C=O of o-quinonediazide group decreased or disappeared after sufficient exposing to light. And also, absorption band C=O of indene carboxylic acid was increased. From the results, it may be concluded that the above photosensi tive polymers were dissociated to indene carboxylic acid soluble in aqueous alkali solution. Further, the effect of sensitizers were examined by the variation of intensity in infrared absorption spectra of sensitized and unsensitized samples.

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Preparation of Amino Acid by Chemical Synthetic Methods (화학합성법에 의한 아미노산의 합성)

  • Son, Tae-Il
    • Applied Chemistry for Engineering
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    • v.4 no.2
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    • pp.254-263
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    • 1993
  • The industry of amino acids has developed for the synthetic methods, zymotechnics from the methods of extraction. In the present, part of amino acids (L-cystine, L-tyrosine, etc.) is manufactured by the methods of extraction, but most of amino acids is produced by synthetic methods, zymotechnics. Among the methods, the synthetic methods use of cyanic acid, which generate a large of waste water by acid or alkali in hydrolysis. In this point of view, improved new synthetic methods are demanded for being the influence of environment. This article Introduces new synthetic methods of phenyl alnine, further more, the recent of research results are introduced to prepare the derivative ${\alpha}-keto$ acids of precursor for preparing more than particular amino acids.

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Effective Interfacial Area in an Agitated Liquid-Liquid Contactor by a Chemical Method (화학방법에 의한 액-액 계면 면적)

  • Park, Sang-Wook;Moon, Jin-Bok;Shin, Jeung-Ho;Park, Dae-Won;Kim, Jong-Hyeon
    • Applied Chemistry for Engineering
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    • v.4 no.1
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    • pp.103-112
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    • 1993
  • The rates of overall mass transfer of n-butyl acetate in the alkaline hydrolysis of n-butyl acetate were measured by using a mechanically agitated vessel in order to get the relationship between the mass transfer rates and experimental variables. The interfacial area between liquid-liquid heterogeneous phases could be obtained by comparing the theoretical values of reaction enhancement factor from an approximated solution of a diffusion equation based on the film theory with the experimental data.

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