• Korean Journal of Plant Resources
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• v.26 no.1
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• pp.103-110
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• 2013

Lacquer has traditionally been used to varnish. Many reports have revealed that lacquer has anti-microbical, anti-cancer, and anti-inflammatory activities. However, urushiol and their derivatives were known as an allergen. Therefore, we expected that lacquer will be used as a good health-food source if its side effect was solved. Here we analyzed their allergy induced constituents by using the GC/MS and evaluated comparative concentration of lacquer, puerariae radix and their mixture extracts. Our results showed that lacquer extract has allergenic compounds and mixture extract with puerariae radix has relatively lower amount. However, lacquer/puerariae mixture extract has strong anti-allergenic effects on the RBL-2H3 cell and puerariae extract was blocked allergenic effect caused by allergenic constituents contained in lacquer.

• Journal of Life Science
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• v.14 no.5
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• pp.847-854
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• 2004

Allergy is a multi-factorial disease influenced by genetic and environmental factors. As the number of allergy-affected people is increasing in developed countries, there is an increasing interest in genetic predisposition to the allergy. A number of genes and chromosomal region have been identified to be linked to allergy including rhinitis, asthma and atopy. In order to understand the genetic background for the allergy-affected people, we investigated genetic predisposition among students enrolled in Busan Science Academy. Among 138 students, about 30% students had some allergy-related disorder including rhinitis, asthma and atopy. We analyzed several single nucleotide polymorphisms (SNPs) within two genes, Inter-leukin-4(IL-4) and Interleukin-4 receptor(IL-4R), which are involved in the induction of allergy reaction with the Th2 immunity. For 96 samples obtained from students, we analyzed 9 SNPs including -590 C/T and -34 C/T in IL-4, and I75V, Q576R, E375A, e406R, 5411L, S761P and S727A in IL-4R. From the analysis, these SNPs showed slight differences among normal and allergy-affected students, but these differences was not enough to predict the predisposition to the allergy. In contrast to previous reports, we could not find SNP(s) related with allergy. These results suggest that genetic tests recently performed in Korea widely have to be reassessed for its validity of genetic predisposition. [Supported by grants from MOST]

• Journal of Life Science
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• v.21 no.12
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• pp.1721-1725
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• 2011

Mast cells are major effector cells associated with allergic responses. They are activated through the release of histamine, arachidonic acid, and proinflammatory cytokines. We investigated the effect of nodakenin, derived from the roots of Angelica gigas Nakai, on mast cell degranulation and on an allergic response in an animal model. We also investigated the effect of nodakenin on expression of multiple cytokines. Nodakenin suppressed the release of ${\beta}$-hexosaminidase, a marker of degranulation, as well as the expression of interleukin IL-4 and TNF-${\alpha}$ mRNA. Nodakenin inhibited the passive cutaneous anaphylaxis (PCA) reaction in ICR mice in a dose-dependent manner. These results suggest that nodakenin can inhibit mast cell degranulation through the inhibition of IL-4 and TNF-${\alpha}$ mRNA expression, and that nodakenin may potentially serve as an anti-allergic agent.

• The Journal of Korean Medicine
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• v.24 no.3
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• pp.65-71
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• 2003

목적 : 정천탕(定喘湯, JCT)이 IgE/mast cell로 유포되는 천식(喘息)등의 알러지성 질환에 대한 효과를 실험적으로 검증하고자 하였다. 방법 : IgE의 혈중농도와(in vivo) 분비 량을 측정하였으며(in vitro), 알러지 관련 유전자의 발현을 RT-PCR로 검색하였고, JCT의 수용성 복합 추출물이 compound 48/80로 유발되는 mast cell line(IC-2)의 histamine의 방출에 미치는 직접적인 영향을 측정하였다(in vitro). 결과 : 정천탕은 ovalbumin으로 유발시킨 마우스의 혈중 IgE량과 IL-4와 CD-40로 유도시킨 spleen B 임파구의 IgE 생성량도 현저하게 감소시켰다. 또한 INF-r의 발현은 증가시킨 반면, IL-4와 IL-6의 발현과 mast cell의 histamine 방출은 억제하는 것으로 나타났다. 결론 : 정천탕의 항알러지 효과를 실험적으로 확인 할 수 있었으며 임상적으로는 천식등 알러지성 질환에 사용 할 수 있을 것으로 사료된다.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.376-379
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• 2004

본 연구는 알레르기 저감화 식품을 개발하기 위한 실용화 방법의 일환으로 실시되었다. 계란으로부터 난백을 분리하여 감마선을 10 및 20 kGy로 조사한 후 케이크를 만들어 알러지원성을 측정하였다. 감마선 조사된 난백으로 만든 케이크에서 추출된 단백질은 전기영동을 통해 확인하였으며 각 시료에 대한 알러지원성은 계란 알레르기가 있는 환자의 혈청을 사용하여 측정한 결과, 난백에 대한 조사 선량이 증가할수록 알러지원성이 감소하는 것을 확인할 수 있었다. 이상의 결과로 볼 때, 감마선 조사된 난백으로 만든 케이크는 대조구에 비해 알러지원성을 감소시킬 수 있었으며 방사선 조사를 통해 알레르기가 저감화된 식품을 개발할 수 있을 것으로 보인다.

• The Journal of the Convergence on Culture Technology
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• v.7 no.1
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• pp.502-510
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• 2021

This study was identified to affection factors the life satisfaction among the college students with allergies by ecological approaches. This study was secondary analysis by using the 2015 Korean Community Health Survey (CHS) raw data, it analyzed 1,862 college students with allergies. Male students' life satisfaction were explained by 7.9% as degree of allergic diseases, frequency of breakfast, networking with friends, social activities with relatives, social activities with religion, satisfaction of natural environment and medical health service. Female students' life satisfaction were explained by 3.0% as frequency of breakfast meal, social activities with relatives and satisfaction of natural environment. We need to make the strategies and policies to improve of student's intake breakfast and make campus' green space.

• Applied Biological Chemistry
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• v.44 no.2
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• pp.133-135
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• 2001

• Journal of Life Science
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• v.20 no.5
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• pp.794-798
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• 2010

Allergic dermatitis is one of the most prevalent diseases in young/juvenile children worldwide. In this research, extracts with persimmon sap-stained fabrics (rayon and cotton) exhibited an elevation in $CD4^+$ cell numbers. MMP-2 and MMP-9 expressions by Hematoxylin-Eosin staining and immunohistochemistry revealed that the expressions were decreased by addition of the extracts. The present results collectively suggest that the active ingredients of persimmon sap-stained fabrics play an important role in inhibition of DNFB-induced-atopic symptoms in vivo.

• Korean Journal of Clinical Laboratory Science
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• v.47 no.4
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• pp.306-312
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• 2015

The measurement of histamine is to determine the degree of allergy because the allergic reaction can lead to the release of histamine. In general, the antigen-antibody reaction was quantified by measuring absorbance using a microplate reader. In this study, we compare the method using a general antigen-antibody reaction and the method using a high performance liquid chromatography mass spectrometer (HPLC-MS) of chemical analysis in the measurement of histamine secretion. The cell line used was RBL-2H3, an allergic reaction was induced by stimulation with C48/80 (compound 48/80). Allergy-induced cells degranulation rate was confirmed by measurement of ${\beta}$-hexosaminidase and cytotoxicity was performed for the validity of the experiment. The quantitative determination of histamine showed a significant difference, since the quantitative limit of the measurement by the antigen-antibody reaction was 10.257 ppm while the quantitative limit of the measurement by HPLC-MS was 0.020 ppm. Measurement of histamine in allergic activity and anti-allergy tests showed that the HPLC-MS analysis rather than the analysis of the antigen-antibody reaction is a more precise and accurate test.

• Journal of the Korean Applied Science and Technology
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• v.37 no.6
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• pp.1764-1772
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• 2020

This study is aimed at determining whether graviola (Annona muricata) beneficially influences immunoglobulin (Ig) E-mediated allergic reactions in human mast cells (LUVA cells). To examine the anti-allergic effect of graviola leaf extract (GLE), we treated antigen/IgE sensitized mast cells with extracts of various concentrations. In this study, the results of the in vitro model of IgE-mediated mast cell degranulation showed that GLE significantly inhibited the release of histamine, β-hexosaminidase, TNF-α, IL-4 and IL-6 in LUVA cells. Pretreatment with GLE suppressed the phosphorylation of antigen-induced Lyn and Syk, thus suppressing the downstream MAPKs pathways. The above results indicate GLE could suppress mast cell activation and allergic responses. Accordingly, it can be supported that GLE has the potential to be used as functional cosmetic material.