• Title/Summary/Keyword: 아마이드

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Polyacrylamide Gel Electrophoresis of the Cell Proteins from Differentiating Aspergillus niger (검정곰팡이의 분화(分化)에 있어서 폴리아크릴아마이드 겔 전기영동법(電氣泳動法)에 의한 단백질 패턴 연구(硏究))

  • Park, Yong-Ja;Kim, Jong-Hyup
    • The Korean Journal of Mycology
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    • v.8 no.3
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    • pp.159-166
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    • 1980
  • Aspergillus niger van Tieghem was cultured by the method of submerged and synchronized culture for the study of differentiation. Acid-phenol soluble cell proteins of the fungus were extracted from four stages during development. Those acid-phenol soluble proteins were separated by polyacrylamide gel electrophoresis to determine the protein patterns. A new protein band was observed from the pre-sporulation body, color density of the stained protein bands in four tubes differed according to the differentiation stages. The number of protein bands in 10% gels varied from 18 to 16, 17, and 19 according to the course of spore germination stage, conidiophore stage, phialide maturation stage and sporulation stage.

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Allele Frequency of the Short Tandem Repeat Locus Human Lipoprotein Lipase(LPL) Gene by Polymerase Chain Reaction in the Korean Population (한국인에서 중합효소연쇄 반응법에 의한 STR 유전좌위 LPL의 유전자빈도 검색)

  • Na, Yun-Ju;Hur, Woong;Yoon, Chang-Lyuk
    • Journal of Oral Medicine and Pain
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    • v.22 no.2
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    • pp.253-260
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    • 1997
  • 한국인 집단에서 개인식별의 기초자료로 활용하고자 한국인 201명을 대상으로 STR 유전좌위 중 하나인 LPL 유전좌위의 유전자 빈도 및 유전자형 분포를 구하였다. 혈액으로부터 추출한 핵 DNA를 중합효소연쇄반응으로 증폭시키고 폴리아크릴아마이드 겔 상에서 전기영동하여 은염색한 후 관찰하여 다음의 결과를 얻었다. 1. 한국인 집단 201명의 LPL 유전자에서 5개의 대립유전자, 7개의 유전자형을 검출하였으며, 이형접합도는 50.7%로 나타났고 대립 유전자다양성 (allelic diversity value)은 0.454, 개 인식 별력 (PD)은 0.674를 보였다. 2. 대립 유전자 및 유전자빈도는 9, 10, 11, 12, 13 대립 유전자에서 각각 0.020, 0.714, 0.100, 0.164, 0.002로 나타났으며, 대립유전자 7, 8, 14는 관찰되지 않았다. 이상의 결과를 볼 때 한국인 집단에서 STR LPL유전좌위의 유전자빈도는 친자감정 등 개인식별에 유용하게 사용할 수 있으나 감정실무에 응용시 다수의 STR유전좌위 및 VNTR유전좌위의 분석을 병행하여야 할 것으로 사료된다.

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Acrylamide monitoring in home-made food products (가정식 음식의 아크릴아마이드 함량분석)

  • Lee Mi-Seon;Park Jae-Young;Oh Sangsuk
    • Korean journal of food and cookery science
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    • v.20 no.6 s.84
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    • pp.708-711
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    • 2004
  • Swedish research in 2002 indicated that acrylamide formation was particularly associated with traditional high temperature cooking processes of certain carbohydrate-rich foods. Since the Swedish report, similar findings have been reported by researchers in numerous other countries. In 2003, three hundred and twenty seven domestic food products, selected on the basis of annual sales in Korea, were analyzed. The results were similar to those from other countries. In this study, the monitoring of acrylamide was expanded to include Korean traditional food products. Samples were purchased from local markets, prepared and then analyzed using LC/MS/MS methods. The categorized food products analyzed included cooked rices and porridges, soup products, fried products, boiled down products, seasoned products, roasted products and coffee. The acrylamide concentrations of the samples were $ND\~18ppb$ in cooked rices and porridges, $ND\~28ppb$ in soup products, $ND\~22ppb$ in fried products, $ND\~218ppb$ in boiled down products, <10ppb in seasoned and roasted products, and <10\~11ppb in brewed coffee and coffee drinks.

Synthesis and Inhibitory Activity on NF-${\kappa}B$ Activation of Chroman-2-carboxylic Acid N-Heteroarylamide Derivatives (크로만-2-카르복실산 N-헤테로아릴아마이드 유도체 합성 및 NF-${\kappa}B$ 저해 활성)

  • Yi, Won-Hui;Kwak, Jae-Hwan;Han, Sang-Bae;Kim, Young-Soo;Jung, Jae-Kyung;Lee, Hee-Soon
    • YAKHAK HOEJI
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    • v.56 no.3
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    • pp.186-190
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    • 2012
  • Nuclear factor-${\kappa}B$ (NF-${\kappa}B$) has been considered as one of the major targets for therapeutic agents of diverse human diseases. In the previous studies, 6-hydroxy-7-methoxychroman-2-carboxylic acid N-phenylamide (KL-1156) and chroman-2-carboxylic acid N-(4-chlorophenyl)amide were identified as good inhibitors of NF-${\kappa}B$ activation. In this continuous study, we describe the synthesis and NF-${\kappa}B$ inhibitory activities of chroman derivatives containing N-heteroaryl groups for exploration of SAR (structure-activity relationship). In addition, inhibitory effects of cell proliferation are evaluated against human cancer cell lines (NCI-H23 and PC-3).

Effects of Potassium Sulfamate on Synthesis of Pottassium Dinitramide (포타슘디나이트라아마이드의 합성에 대한 술팜산칼륨의 영향 연구)

  • Kim, Woo-Ram;Huang, Shan;Kwon, Youn-Ja;Jo, Young-Min;Park, Young-Chul
    • Journal of the Korean Applied Science and Technology
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    • v.30 no.1
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    • pp.57-63
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    • 2013
  • Ammonium dinitramide (AND) is one of the most promising oxidizers in propellants without chlorinated pollution to the atmosphere in these days. Potassium sulfamate (PS) is a key substance in ADN synthesis as forming nitrates such as $-N(NO_2)_2$. In this paper, potassium sulfamate as a starting material for ADN synthesis was prepared in a lab scale through crystallization with ethanol solvent, and observed the effects on the yield and purity of KDN. The prepared potassium sulfamates were analyzed using FE-SEM, XRD, BET and TGA-DSC. The lab-made PS, which was ground to $20{\mu}m$ showed more beneficial than a commercial product achieving high yield and purity of the synthesized KDN. It would be associated closely with crystallinity, porosity and pore size of prepared PS.

Gelation Behavior of Acrylonitrile Copolymer/Dimethylformamide Solution and Mechanical Properties of Films Obtained from It′s Solution (아크릴로니트릴공중합체-디메틸포름아마이드 용액의 겔화 거동 및 겔필름의 물성)

  • 오영세;한삼숙;송기원
    • Polymer(Korea)
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    • v.24 no.6
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    • pp.787-793
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    • 2000
  • The acrylonitrile copolymer/dimethylformamide (DMF) solutions were prepared to investigate the gelation behavior and critical gel concentration (c*). Gelation is rapidly progressed with the increase of molecular weight of copolymer, but significantly delayed with supercooling temperature and comonomer contents. The c* behavior showed contrary trend against gelation behavior. In dynamic viscoelastic test, two glass-transition region were observed in film obtained from gelled solution whereas one glass-transition in film obtained from true solution. This result supports the idea that an ordered junction zone is formed by the dipole-dipole interaction of intermolecularly neighboring stereo-regular parts of atactic acrylonitrile copolymer chains due to a nucleation process in the solution.

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Preparation of Titania Nanotube Thin films by Anodizing (양극산화를 이용한 Titania Nanotube(TNT) 박막 제조)

  • Lee, Young-Rok;Jung, Ji-Hoon
    • Korean Chemical Engineering Research
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    • v.49 no.1
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    • pp.28-34
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    • 2011
  • Titania nanotube(TNT), which is a tube shaped thin film manufactured by anodizing titanium under $F^-$ ion electrolyte, has photo activity. Distilled water and formamide were used as solvent, and HF, NaF, $NH_4F$ were used as main $F^-$ ions for the electrolyte. The length and the diameter of TNT increased as the voltage and anodizing time increased. TNT prepared by anodizing was a very ordered tube, and had a maximum length of 13.7 ${\mu}m$ depending on the conditions of manufacturing. Titania prepared by anodizing was amorphous, and became an anatase crystal after heat treatment.

Permeation Properties of Insulin through Chitosan-g-Poly(4-vinyl pyridine ) Membrane (Chitosan-g-Poly(4-vinyl pyridine) 공중합체막을 통한 인슐린의 투과 특성)

  • Kim, Kong Soo;Kang, Seog Ho;Kim, Soo Jong;Lim, Jeong Kyu
    • Applied Chemistry for Engineering
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    • v.5 no.4
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    • pp.624-629
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    • 1994
  • Chitosan-g-poly(4-vinyl pyridine)membranes were prepared by crosslinking reaction using glutaraldehyde and glucose oxidase immobilized chitosan/acrylamide composite membranes were fabricated by chitosan-g-poly(4-vinyl-pyridine) copolymer and acrylamine. Water content and permeability of insulin through chitosan-g-poly(4-vinyl pyridine )membranes increased with decreasing the pH of the medium. Permeability of insulin through chitosan/acrylamide composite membranes increased with increasing the concentration of glucose.

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Reaction of Potassium Fluoride with Orgarnic Halogen Compound-II. Dimerization, Fluorination, and Decarboxylation of Organic Iodo acids, Iodides, and Polychlorinated Acid Ester (有機할로겐化合物과 KF 과의 反應 (第2報) 有機요-드酸, 沃化物 及 多鹽素化有機酸, 에스텔의 雙合, 弗化 及 脫炭酸 反應에 關하여)

  • Kim, You-Sun
    • Journal of the Korean Chemical Society
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    • v.8 no.4
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    • pp.135-141
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    • 1964
  • The reaction of potassium fluoride with amyl iodide in presence of dimethylformamide at low temperature gave a fluorination product together with decane. Polyhalogenated acid such as ${\alpha}$, ${\beta}$,-dichloro-${\beta}$-phenyl-propionic acid gave ${\alpha}$-chlorostyrene, fluorinated styrene, and fluorinated acid. The same reaction with the ethyl ester did not give the fluorination product and gave a mixture of various dimerized product. Dibromostyrene gave bromostyrene together with fluorination product. Iodo acid such as m-iodo acid gave the salt and a trace quantity of dimerized product. Iodides such as m-iodotoluene and 1-amino-4-iodo-2-methyl benzene did not show any appreciable reactivity towards potassim fluoride. The reaction condition was described, and fluorination, ${\alpa}$-dehydrofluorination, decarboxylation, and dimerization were discussed.

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Use of Flour-Impregnated Polysulfone Membranes for Measuring Radioactive Contamination in Laboratories (실험실 방사성핵종 오염도 측정에 있어 Fluor 함침 폴리설폰 막의 이용)

  • 한명진
    • Membrane Journal
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    • v.9 no.2
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    • pp.132-139
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    • 1999
  • Solid scintillation proximity membranes were prepared for measuring the amount of radioactivity in laboratories contaminated by the radionuclide of $^3H$-cortisol. The membranes, consisting of polysulfone as a polymer matrix and cerium activated yttrium silicate as a fluor, were used to monitor the amount of radioactivity without the aid of a scintillation cocktail required for the conventional wipe test. The test results of the cocktail-free wipe test showed that the prepared membranes were efficient to monitor radionuclide-contaminated areas with the good counting ability as well as with the decrease of overall production of radioactive waste. On the other hand, solvent treatment of the prepared membranes could induce a significant variation of membrane morphology, but the counting efficiency of the solvent-treated membranes was not improved than that of the untreated one.

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