• Proceedings of the Korean Society of Applied Pharmacology
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• 2005.04a
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• pp.65-79
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• 2005

• Journal of Sericultural and Entomological Science
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• v.48 no.2
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• pp.56-60
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• 2006

This study was performed to investigate the effect of silk protein (BM-1) treatment on epidermal growth factor (EGF) expression in the wound healing process by excision on the hairless mouse. Significant wound healing activity was observed BM-1 treated group. In BM-1 treated groups ($100{\sim}116 ug/day$), epithelialization of the incision wound was laster with a high rate of wound contraction. In expression of EGF and EGF mRNA, the lesion of BM-1 treated group made EGF to more induce significantly than control lesion. These data suggest that silk protein (BM-1) treatment have wound healing effect by excision on the hairless mouse.

• Journal of Sericultural and Entomological Science
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• v.49 no.2
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• pp.67-70
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• 2007

This study was done for the dietary effect of silk protein, molecular controlled silk sericin and silk fibroin, on colon cancer of animal. The ICR mice were given weekly injections of 1,2-dimethylhydrazine (DMZ) for the initial 10 mg/kg with four weeks, and fed a diets supplemented with 3% (v/v) molecular controlled sericin and fibroin for 6 weeks, respectively. Then, the body weight, efficiency of dietary, typical histologic appearance of animal colon tissue and COX-2 effect were studied, too. Molecular controlled sericin protein are shown more higher dietary effect on animal colon cancer than fibroin supplemental case. The results suggest a potential usefulness of sericin as a chemopreventive for colon carcinogenesis and the development functional food.

• Polymer(Korea)
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• v.35 no.4
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• pp.289-295
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• 2011

Biomaterials for retinal tissue engineering must demonstrate several critical features for potential utility, including mechanical integrity, biocompatibility, and slow biodegradation. Silk film biomaterials were designed and characterized to meet these functional requirements. We prepared natural/synthetic hybrid silk/PLGA films using 0, 10, 20, 40, and 80 wt% of silk by a solvent evaporation method. MIT assay was used to confirm the number of cells attached on film at 1, 2, and 3 days, respectively. The morphology of cellular adhesion on films was also confirmed by scanning electron microscope (SEM). RT-PCR was conducted to confrrm mRNA expression of retinal pigment epithelitun (RPE) using RPE65 as a RPEs marker and the expression of cytokeratin were determined by immunofluorescence staining. We confirmed that the silk/PLGA film of 20~40 wt% silk was superior for the adhesion and proliferation of RPEs.

• Polymer Science and Technology
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• v.16 no.5
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• pp.542-555
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• 2005

• Proceedings of the Korean Fiber Society Conference
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• 2003.04a
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• pp.293-294
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• 2003

견 피브로인은 대표적인 섬유상 단백질의 하나로 생체적합성, 생분해성, 저독성 등의 유용한 특성을 가지므로 생체재료로 상당한 관심과 연구의 대상이 되어왔다. 우리는 최근의 연구에서 견 피브로인을 생사로부터 추출한 다음, 포름산을 용제로 하여 전기방사함으로써 나노섬유를 제조하고 이들의 각화세포에 대한 친화력을 확인한 바 있다. 본 연구에서는 견 피브로인의 구조체를 나노섬유 부직포, 필름, 마이크로 섬유로 구성된 직물 둥의 형태로 하여 그들의 2차 구조를 비교함과 동시에 구조적 특성이 각화세포와의 친화력에 어떠한 영향을 미치는 가를 비교ㆍ검토 하고자 하였다. (중략)

• Proceedings of the Korean Fiber Society Conference
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• 2003.10b
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• pp.281-282
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• 2003

견피브로인의 우수한 물리화학적 성질에 기인하여 실크는 과거로부터 지금까지 인류에게 매력적인 소재로 인식되고 있다. 견피브로인은 주로 의류용 소재로서 이용되고 있지만 현재에는 생명공학용 소재로서의 가치가 크게 부각되고 있어서 그 가치는 더욱 크다고 할 수 있다. 따라서 이를 용도에 적합한 형태로 성형하고자 하는 연구가 계속되고 있다. 성형의 한 방법으로 재생 견피브로인 필라멘트에 대해서도 많은 연구가 이루어지고 있다. (중략)

• Journal of Sericultural and Entomological Science
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• v.52 no.2
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• pp.117-122
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• 2014

On previous studies, we constructed a transgenic silkworm which produces the chimeric silk fused green fluorescent protein (EGFP), but the transgenic silkworm has decreased commercial feasible traits such as convenience of breeding and productivity of silk. In this study, we performed cross fertilization between green fluorescent silk transgenic silkworm and colored cocoon silkworm descents to make the transgenic the transgenic silkworm producing improved fluorescence cocoon. In the result, we found out a bit valuable cross fertilization manners ($female{\times}male$) in respect of silk productivity such as $T59B{\times}Jam26$, $Jam329{\times}T59W$, $T59W{\times}Jam329$, and $T59W{\times}Jam178$. The color-difference of offspring cocoons were measured according to different cross manners using by CIE Lab-based formulae with a X-rite VS450. In the result, the depth of green color of cocoons was a little high at cross manners as $Jam329{\times}T59W$, $T59W{\times}Jam178$. Meanwhile, the depth of yellow clolor of cocoons was remarkable at cross manners as $Jam178{\times}T59W$, $T59W{\times}Jam178$, respectively.

• Membrane Journal
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• v.17 no.4
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• pp.375-380
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• 2007

To recover sericin protein from by-product in silk production process, a polyethersulfone hollow fiber ultra-filtration membrane module was used. The soap in the degummed solution was precipitated by calcium chloride. The influence of membrane module of submerged and external type on membrane fouling was investigated. The effect of soap and protein on the membrane fouling in the external type membrane module was also studied. The removal of soap resulted in decreasing the membrane fouling. It was shown that the protein and the membrane were affected by the soap.

• Microbiology and Biotechnology Letters
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• v.30 no.1
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• pp.39-45
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• 2002

It was found that the peptides originated from the hydrolysates of silk fibroin have in vitro immunostimulating effects in murine macrophage RAW264.7 cells. The stimulation effects on nitric oxide (NO) production resulted from treatments of acid or enzymatic hydrolysates were measured. The silk fibroin preparation isolated from cocoon was most efficiently digested by acid hydrolysis. Even though the sole treatment of acid hydrolysate stimulated the NO production in dose-dependent pattern, a part of its activity was found to be caused by the contaminated endotoxin, LPS. When each endotoxin-free hydrolysates obtained by filtering it through an ultrafiltration membrane of molecular weight (MW) cut-off 10,000 to eliminate LPS was used, the peptic hydrolysate with lowest degree of hydrolysis showed the highest activity. The fractions of peptic hydrolysate with MW ranges of 1,000∼10,000, 500∼1,000 and below 500 also showed a higher MW-higher activity correlation. From the analyses of amino acid composition of each hydrolysate, it was found that the contents of arginine, lysine, alanine and glycine residues affected the activity level of hydrolysate. The results of this study showed a possibility of utilizing fibroin as a source for immunostimulating (chemopreventive) functional peptides.