• Title/Summary/Keyword: 실내용기

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Studies on the Life History of Three Spotted Plusia (Chrysoideixis agnata S.) in the laboratory (콩은무늬밤나방의 생활사에 관한 연구)

  • Hwang C.Y.;Uhm K.B.;Choi K.M.;Hyun J.S.
    • Korean journal of applied entomology
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    • v.19 no.2 s.43
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    • pp.85-89
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    • 1980
  • This experiment was carried out to investigate the life history of the Three spotted plusia (Chrysodeixis agnata Staudinger) in the laboratory in 1976. (1) Female deposited $1202{\pm}150.6$ eggs during the oviposition period which was $13.3{\pm}1.2$ days. (2) Especially, larvae were distinguished with 5-instar type and 6-instar type. The egg period was 2 days and adult longevity was $21.29{\pm}3.15$ days for female and $19.14{\pm}2.47$ days for male. Larval period and pupal period were $15.36{\pm}1.59,\;7.82{\pm}1.01$ days for the 5-instar type and $16.92{\pm}1.08,\;8.23{\pm}1.09$ days for the 6-instar type. (3) Width of head capsule of the 5-instar type was $2.77\~2189mm$ and this of the 6-instar type was $2.78\~22.38mm$. (4) A highly significant correlation was recognized between the log-width of head capsule and the number of instar.

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A Study on the Ovipositional Characteristics of Endangered Species Prosopocoilus astacoides blanchardi (Coleoptera: Lucanidae) for Indoor Mass-rearing (멸종위기종 두점박이사슴벌레의 실내 증식을 위한 산란 특성 연구)

  • Ko, Gyeong Hun;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.423-429
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    • 2021
  • The ovipositional characteristics of the endangered species Prosopocoilus astacoides blanchardi was investigated for indoor mass-rearing. This insect required 3 weeks or more for mating after emergence when introduced at the 1:1 ratio of male to female. Among temperatures studied at 16, 20, 24, 28, and 32℃, the pre-ovipositional period of P. astacoides blanchardi was longest at 16℃ with an average of 109 days after emergence, while it was shortest at 32℃ with an average of 59.4 days. The number of eggs laid at 24℃ was highest with an average of 40.6 per female. The average lifespan of adults at 16℃ was 208.8 days for males and 263.9 days for females, while at 32℃ 96.1 days for males and 130.3 days for females. The number of laid eggs was dependent on insect rearing chamber size. The oviposition-trees did not significantly (P > 0.05) affect the number of laid eggs and ovipositional period. The results of this study could be used as rearing data for the restoration and mass propagation of the endangered species P. astacoides blanchardi.

Selection of Bactericides for Controlling Soybean Bacterial Pustule (콩 불마름병 방제를 위한 살균제 선발)

  • Hong, Sung-Jun;Kim, Yong-Ki;Jee, Hyeong-Jin;Lee, Bong-Choon;Yoon, Young-Nam;Park, Sung-Tae
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.266-273
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    • 2010
  • Bacterial pustule of soybean (Glycines max) caused by Xanthomonas axonopodis pv. glycines is one of the most prevalent bacterial diseases of soybean. This bacterium shows strong pathogenicity to the plants and distributes throughout Korea. However, no good control measures including bactericides and resistant cultivars are available to control the disease in Korea. Therefore, this study was conducted to develop chemical control method against soybean bacterial pustule. The present study was undertaken to find out the growth inhibitory effect bactericides (8 antibiotics, 2 copper compounds, quinoline, 18 agro-chemicals) on bacterial pustule pathogen. Antibiotics test showed that tetracycline and streptomycin sulfate significantly suppressed the growth of bacterial pustule pathogen. Also, application of oxolinic acid was found to be effective for pathogen inhibition. However, vancomycin, polymyxin B sulfate and copper compounds did not show the positive suppressive effect on growth of the pathogen. Among the eighteen agro-chemicals, streptomycin sulfate + oxytetracyclin (18.8 + 1.5%) WP, oxytetracycline (17%) WP and oxolinic acid (20%) WP were found to be effective for the inhibition of the pathogen in vitro. The selected 5 agro-chemicals were also applied on soybean in field and their control effects against the soybean bacterial pustule were tested. The foliar application of streptomycin sulfate + oytetracyclin WP and oxytetracycline WP on the naturally infected soybean (Taekwangkong) showed high control value (above 70%). Therefore, it is concluded that the bactericides used in this study showed strong inhibitory effect to soybean bacterial pustule and they can be recommend to farmers to control the disease.

Rearing Method for Ascotis selenaria (Lepidoptera: Geometridae) using an Artificial Diet (인공사료를 이용한 네눈쑥가지나방(Ascotis selenaria)(나비목: 자나방과) 실내 사육법)

  • Choi, Kyung-San;Park, Young-Mi;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.50 no.1
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    • pp.55-63
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    • 2011
  • This study was conducted to develop an artificial diet for the mugwort looper, Ascotis selenaria (Lepidoptera: Geometridae), which is an insect pest to leaves of citrus (Citrus unshiu). Corn and soybean powder were selected as main nutrient sources for larvae of A. selenaria after several diets consisted of wheat germ, corn, kidney bean and/or soybean were tested for larval development and survival. A higher amount of the main nutrients in the diet increased the larval survivorship. Addition of yeast and cholesterol in diet increased the larval survivorship. Finally the composition of diet was decided as followings; corn 100 g, soybean 100 g agar 25 g, Brewers' yeast 30 g, cholesterol 0.5 g, Vanderzant vitamin mixture 2 g, Wesson's salt mixture 2 g, sorbic acid 2 g, ascorbic acid 2 g, and methyl-4-hydroxybenzoate 2.5 g, and distilled water 1 liter. Development periods of larvae and pupae, survival rate and fecundity of A. selenaria reared on the diet were not significantly different with those on the host plant, citrus leaves. Larvae of early instars were reared in a group, while larvae of later instars (5-6th) were reared individually. Adult mating was conducted in a plastic cage and an oilpaper covered with a gauze was provided as an oviposition site.

Development of Semi-automatic Cabbage Piling System for Tractor Implemented Chinese Cabbage Hervester (트랙터 부착형 배추 수확기용 반자동식 배추 적재시스템 개발)

  • Song, K. S.;Choi, D. Y.;Hwang, H.
    • Journal of Biosystems Engineering
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    • v.27 no.3
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    • pp.211-218
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    • 2002
  • 배추 생산에 있어서 수확, 운반, 적재 작업은 가장 노동이 집약적으로 요구되는 작업들이다. 최근, 여러 종류의 양배추 수화기가 일본과 유럽에서 개발되었다. 하지만 국내에서 재래되는 배추는 크기와 형태에 있어 양배추와는 달라 기 개발 기종의 도입이 어렵다. 또한 수확작업의 생력화 효과는 운반, 정선, 적재 작업과 밀접하게 연계되어 있어 출하시의 작업체계를 고려하여 수화에 따른 수집.반출 시스템을 개발하여야 한다. 수확시의 배추는 중량이 25~45 N 정도로 다 작물에 비하여 무겁고 부피가 크기 때문에 수확작업의 기계화를 위해서는 수확장치와 더불어 연속적으로 수확되는 배추를 적절하게 수집하여 적재하고 반출하는 시스템의 개발이 중요하다. 본 연구에서는 수확작업의 생력화 효과를 높이고 생력화 시스템 비용의 절감을 목적으로 작업자 1인에 의해 작업할 수 있는 반자동 형태의 트랙터 부착형 배추 수집, 적재, 반출시스템 시작기를 개발하였다. 시작기는 배추 이송장치, 적재장치, 팔렛 및 반출장치의 3개 부분과 PLC를 이용한 주 제어기로 구성하였다. 배추 수집용기로는 대략 70개의 배추를 담을 수 있는 크기가 1,050 mm$\times$1.050mm$\times$1,000mm 인 접이식 메쉬 팔렛을 사용하였으며 하단부에 롤러 안내판을 부착하여 적재한 팔렛의 배출이 용이하도록 하였다. 팔렛을 제외한 전체 시작기의 중량은 235 N 이였으며 크기는 3,940mm$\times$520mm$\times$1,630mm 이었다. 본 연구는 수확장치의 기능 및 생력화 효과를 극대화하고 배추의 손상정도를 최소화하는 시스템을 구성하고자 하였다. 이송장치는 트랙터 부착시 횡공간 점유율을 최소화하도록 하였으며 적재장치는 적재시 배추의 손상을 줄이고 배추가 놓이는 자세를 능동적으로 조절할 수 있도록 주름관을 부착하였다. 시작기의 실내시험 결과 이송장치는 0.18 m/s~0.36 m/s의 범위에서 적재장치는 0.4 m/s~2.4 m/s 범위에서 안정적으로 구동하였으며 두 장치를 동시에 구동하여 시험한 결과 이송장치는 0.26 m/s~0.36 m/s, 그리고 적재장치는 0.9 m/s~2.4 m/s 에서 적정하게 안정적으로 구동하였다. 적재장치의 성능에 있어서 1~3단 적재시에는 주름관을 이용하여 적재하고 4~5단 적재시에는 자유낙하에 의한 적재를 수행할 경우 인력에 의한 적재와 거의 동등한 적재량을 보였으며 손상정도는 거의 무시할 정도였다. 트랙터가 0.3 m/s로 주행하는 경우 노지로부터 배추를 뽑아 이송하는 뽑기벨트의 적정속도가 0.46 m/s인 점을 고려할 때 배추 이송 컨베이어는 0.34 m/s 이상의 속도를 유지할 필요가 있었으며 적재 컨베이어는 2 m/s~2.4 m/s의 속도에서 안정적으로 작동하였다. 배추의 주간 거리가 대략 30~40 cm 인 점을 감안하면 적재장치는 초당 1개의 적재성능을 보였다. 실내에서 수행한 시스템의 성능은 배추에 큰 손상없이 전반적으로 성공적으로 구동하였으나 향후 노면이 고르지 못한 포장에서의 성능 시험이 필요하다.

Suppressive Effect of Repellent Plant Cultivation against Striped Flea Beetle of Chinese Cabbage (동반작물 주위재배에 의한 배추 벼룩잎벌레 억제효과)

  • Han, Eun-Jung;Choi, Jae-Pil;Kim, Yong-Ki;Hong, Sung-Jun;Park, Jong-Ho;Shim, Chang-Ki;Kim, Min-Jeong;Kim, Seok-Cheol
    • Korean Journal of Organic Agriculture
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    • v.23 no.4
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    • pp.911-921
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    • 2015
  • We evaluated two herbal plants, basil (Ocimum basilicum) and lemon balm (Melissa officinalis), for their potential as repellent plants in the management of striped flea beetle (Phyllotreta striolata), in organic production of chinese cabbage. It was confirmed that striped flea beetle avoided these two herbal plants in olfactometer experiment. We conducted field experiment in which the suppressive effect of the two selected herbal plants and cruciferous crops preferred by striped flea beetle were evaluated through cultivating as inter crops and border crops, respectively. In inter-cropping experiment, unlike in vitro test, the density of striped flea beetle was low in basil- or mustard-intercropping cultivation plot, but high in lemon balm-intercropping cultivation plot. In border cropping experiment, the density of striped flea beetle on chinese cabbage in lemon balm or basil cultivation plot were approximately zero. As a result of this study, we think that border cropping of lemon balm or basil can be used to reduce preventively striped flea beetle of chinese cabbage under organic farming system.

Developmental Characteristics of Soybean Podworm, Matsumuraeses phaseoli (Lepidoptera: Tortricidae) and Legume Pod Borer, Maruca vitrata (Lepidoptera: Pyralidae) on Semi-synthetic Artificial Diets (반합성 인공사료에서 팥나방(Matsumuraeses phaseoli)과 콩명나방(Maruca vitrata)의 발육 특성)

  • Jung, Jin-Kyo;Seo, Bo-Yoon;Park, Jong-Ho;Moon, Jung-Kyung;Choi, Bong-Su;Lee, Young-Ho
    • Korean journal of applied entomology
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    • v.46 no.3
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    • pp.393-399
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    • 2007
  • Two lepidopteran species, Matsumuraeses phaseoli (Matsumura) and Maruca vitrata (syn. M. testulalis) (Fabricius) were reared on artificial diets, and analyzed in their developmental characteristics. Photoperiod was supplied with 16L/8D for M. phaseoli and with 13L/11D for M. vitrata, respectively. Both species passed five larval instars with discrete sizes of head capsule width. In a constant environment ($25^{\circ}C$ and 65%RH), the developmental period of M. phaseoli egg, larva and pupa was 3.9, ca. 16.0 and 8.9 days, respectively, and over 80% of M. phaseoli larvae could develop into pupae, most of which emerged into adults. Newly laid eggs could be stored at $5^{\circ}C$ for 15 days with over 50% hatchability. Similar developmental traits were shown in M. vitrata. However, a low temperature preservation was not applicable to M. vitrata eggs.

Consequence Analysis for Accidental Gas Release in Labs (실험실 가스 누출 시 피해 영향 분석)

  • Jang, Yuri;Jung, Seungho;Park, Kyoshik
    • Journal of the Korean Institute of Gas
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    • v.19 no.4
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    • pp.29-34
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    • 2015
  • Accidents in laboratory dealing with chemicals have constantly occurred. In the case of a gas explosion or an accident related to leakage of chemical materials, the damage is much greater, thereby leading to a serious accident. Especially, the safety of laboratory in University is important because students build up knowledge and skills and accumulate experience as the main researchers. In this paper, 5 gases(CO, $NH_3$, $H_2$, $CH_4$, $N_2$) are selected to model since they are often used in university laboratories. From the scenarios where the gases are released, the diffusion process is estimated and analyzed to predict damage degree by PHAST v.6.7. Internal diffusion process is modeled through FLUENT which is Computational Fluid Dynamics(CFD) tool. Also, we compare indoor damage with outdoor one when discharged to the outside through the laboratory's window. In the modeling results, the outdoor damages for accident scenarios in the results are far less than then of real plants since the vessel usually used in laboratory(i.e. the capacity of the cylinder; 47 L or less) is significantly less than workplace's one(using ton measure). However as shown in the results small amount can have high consequences for indoor accidents.

Study on Stable Fly Eradication by :Sterile-Male Technique. 1) Mass Rearing of the Stable Fly. Stemoxys calcitrans L. (웅성불임기술을 이용한 쇠파리 구제에 관한 연구 1) 쇠파리의 인공대량사육에 관하여)

  • Chung K. H.;Ryu J.;Kim Y. R.;Kwon S. H.;Park J. D.;Kang T. S.
    • Korean journal of applied entomology
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    • v.12 no.1
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    • pp.41-46
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    • 1973
  • 1) The optimum temperature for mass rearing of stable fly was $26^{\circ}C$ centigrade. Number of days required for stage of development at $26^{\circ}C$ were 6.8 days for larval stage, 5.3 days for pupla stage, 10.4 days for preovipositionla stage, and 30 days for adult stage respectively. 3) The pupation rate, emergence rate and sex ratio were $80.7\%,\;84.3\%$ and 1 : 1, respectively. 3) The average weight of pupae was 14.5mg, and the standard medium showed better result in larvae rearing than wheat bran medium. 4) The optimum number of eggs for inoculation on 125gr medium was approximately 310. 5) Optimum size of resting place was determined as $2inch^2/adult$ when it reared in a rectangular cage.

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Development Period and Oviposition of Pseudogonatopus nudas Perkins (Hymenoptera : Dryinidae), a Nymphal Parasitoid of th Whitebacked Planthoppe, Sogatela furcifera Horvath (Homoptera : Delphacidae) (흰등멸구에서 약충기생벌(Pseudogonatopus nudas Perkins)의 발육기간과 산란수 조사)

  • 김정부
    • Korean journal of applied entomology
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    • v.29 no.3
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    • pp.165-169
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    • 1990
  • A study was carried out to investigate some biological characteristics of Pseudogonatopus nudas, a nymphal parasitoid of the whitebacked planthopper (WBPH) under laboratory conditions (26-$28^{\circ}C$). Developmental periods of eggs, larva, prepupa and pupa were 2.5, 7.8, 2.7, and 14.2 days in average, respectively. Total developmental period from egg to adult emergence was average of 27.2 days. More than 83.5% of the wasps emerged in the morning between 6 and 10 o'clock, while very few emerged in the afternoon. The average longevity of female and male were 11.8 and 3.4 days when honey and WBPH nymphs were supplied as food. female laid an average of 25.3 eggs and the oviposition period was 5.4 days. The parasitoid seemed to prefer the 3rd instar nymphas as the host for oviposition with 68.8%, followed by the 2nd (19.6%), 4th (11.05) and 5th instar (0.6%). And no parasitism was recorded from the 1st instar nymphs and in adults.

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