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Development of Analytical Method for Fenoxycarb, Pyriproxyfen and Methoprene Residues in Agricultural Commodities Using HPLC-UVD/MS (HPLC-UVD/MS를 이용한 농산물 중 fenoxycarb, pyriproxyfen 및 methoprene의 분석법 확립)

  • Lee, Su-Jin;Kim, Young-Hak;Song, Lee-Seul;Hwang, Yong-Sun;Lim, Jung-Dae;Sohn, Eun-Hwa;Im, Moo-Hyeog;Do, Jung-Ah;Oh, Jae-Ho;Kwon, Ki-Sung;Lee, Joong-Keun;Lee, Young-Deuk;Choung, Myoung-Gun
    • The Korean Journal of Pesticide Science
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    • v.15 no.3
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    • pp.254-268
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    • 2011
  • Fenoxycarb, pyriproxyfen and methoprene are juvenile hormone mimic insecticide. These insecticides have been widely used for mosquito, fly, scale insects, and Lepidoptera. The purpose of this study was to develop a simultaneous determination procedure of fenoxycarb, pyriproxyfen and methoprene residues in crops using HPLC-UVD/MS. These insecticide residues were extracted with acetone from representative samples of four raw products which comprised brown rice, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and then n-hexane/dichloromethane partition was followed to recover these insecticides from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The analytes were quantitated by HPLC-UVD/MS, using a $C_{18}$ column. The crops were fortified with each insecticide at 3 levels per crop. Mean recovery ratios were ranged from 80.0 to 104.3% in four representative agricultural commodities. The coefficients of variation were less than 4.8%. Quantitative limit of fenoxycarb, pyriproxyfen, and methoprene was 0.04 mg/kg in crop samples. A HPLC-UVD/MS with selected-ion monitoring was also provided to confirm the suspected residues. The proposed simultaneous analysis method was reproducible and sensitive enough to determine the residues of fenoxycarb, pyriproxyfen and methoprene in the agricultural commodities.

A Comparative Study on the Possibility of Land Cover Classification of the Mosaic Images on the Korean Peninsula (한반도 모자이크 영상의 토지피복분류 활용 가능성 탐색을 위한 비교 연구)

  • Moon, Jiyoon;Lee, Kwang Jae
    • Korean Journal of Remote Sensing
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    • v.35 no.6_4
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    • pp.1319-1326
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    • 2019
  • The KARI(Korea Aerospace Research Institute) operates the government satellite information application consultation to cope with ever-increasing demand for satellite images in the public sector, and carries out various support projects including the generation and provision of mosaic images on the Korean Peninsula every year to enhance user convenience and promote the use of satellite images. In particular, the government has wanted to increase the utilization of mosaic images on the Korean Peninsula and seek to classify and update mosaic images so that users can use them in their businesses easily. However, it is necessary to test and verify whether the classification results of the mosaic images can be utilized in the field since the original spectral information is distorted during pan-sharpening and color balancing, and there is a limitation that only R, G, and B bands are provided. Therefore, in this study, the reliability of the classification result of the mosaic image was compared to the result of KOMPSAT-3 image. The study found that the accuracy of the classification result of KOMPSAT-3 image was between 81~86% (overall accuracy is about 85%), while the accuracy of the classification result of mosaic image was between 69~72% (overall accuracy is about 72%). This phenomenon is interpreted not only because of the distortion of the original spectral information through pan-sharpening and mosaic processes, but also because NDVI and NDWI information were extracted from KOMPSAT-3 image rather than from the mosaic image, as only three color bands(R, G, B) were provided. Although it is deemed inadequate to distribute classification results extracted from mosaic images at present, it is believed that it will be necessary to explore ways to minimize the distortion of spectral information when making mosaic images and to develop classification techniques suitable for mosaic images as well as the provision of NIR band information. In addition, it is expected that the utilization of images with limited spectral information could be increased in the future if related research continues, such as the comparative analysis of classification results by geomorphological characteristics and the development of machine learning methods for image classification by objects of interest.

Using Trophic State Index (TSI) Values to Draw Inferences Regarding Phytoplankton Limiting Factors and Seston Composition from Routine Water Quality Monitoring Data (영양상태지수 (trophic state index)를 이용한 수체 내 식물플랑크톤 제한요인 및 seston조성의 유추)

  • Havens, Karl E
    • Korean Journal of Ecology and Environment
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    • v.33 no.3 s.91
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    • pp.187-196
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    • 2000
  • This paper describes a simple method that uses differences among Carlson's (1977) trophic state index (TSI) values based on total phosphorus (TP), chlorophyll a (CHL) and Secchi depth (SD) to draw inferences regarding the factors that are limiting to phytoplankton growth and the composition of lake seston. Examples are provided regarding seasonal and spatial patterns in a large subtropical lake (Lake Okeechobee, Florida, USA) and inter- and intra-lake variations from a multilake data set developed from published studies. Once an investigator has collected routine water quality data and established TSI values based on TP, CHL, and SD, a number of inferences can be made. Additional information can be provided where it also is possible to calculate a TSI based on total nitrogen (TN). Where TSI (CHL)<>TSI (SD), light attenuating particles are large (large filaments or colonies of algae), and the phytoplankton may be limited by zooplankton grazing. Other limiting conditions are inferred by different relationships between the TSI values. Results of this study indicate that the analysis is quite robust, and that it generally gives good agreement with conclusions based on more direct methods (e.g., nutrientaddition bioassays, zooplankton size data, zooplankton removal experiments). The TSI approach, when validated periodically with these more costly and time-intensive methods, provides an effective, low cost method for tracking long-term changes in pelagic structure and function with potential value in monitoring lake ecology and responses to management.

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Estimation of river discharge using satellite-derived flow signals and artificial neural network model: application to imjin river (Satellite-derived flow 시그널 및 인공신경망 모형을 활용한 임진강 유역 유출량 산정)

  • Li, Li;Kim, Hyunglok;Jun, Kyungsoo;Choi, Minha
    • Journal of Korea Water Resources Association
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    • v.49 no.7
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    • pp.589-597
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    • 2016
  • In this study, we investigated the use of satellite-derived flow (SDF) signals and a data-based model for the estimation of outflow for the river reach where in situ measurements are either completely unavailable or are difficult to access for hydraulic and hydrology analysis such as the upper basin of Imjin River. It has been demonstrated by many studies that the SDF signals can be used as the river width estimates and the correlation between SDF signals and river width is related to the shape of cross sections. To extract the nonlinear relationship between SDF signals and river outflow, Artificial Neural Network (ANN) model with SDF signals as its inputs were applied for the computation of flow discharge at Imjin Bridge located in Imjin River. 15 pixels were considered to extract SDF signals and Partial Mutual Information (PMI) algorithm was applied to identify the most relevant input variables among 150 candidate SDF signals (including 0~10 day lagged observations). The estimated discharges by ANN model were compared with the measured ones at Imjin Bridge gauging station and correlation coefficients of the training and validation were 0.86 and 0.72, respectively. It was found that if the 1 day previous discharge at Imjin bridge is considered as an input variable for ANN model, the correlation coefficients were improved to 0.90 and 0.83, respectively. Based on the results in this study, SDF signals along with some local measured data can play an useful role in river flow estimation and especially in flood forecasting for data-scarce regions as it can simulate the peak discharge and peak time of flood events with satisfactory accuracy.

Comparison of Forest Carbon Stocks Estimation Methods Using Forest Type Map and Landsat TM Satellite Imagery (임상도와 Landsat TM 위성영상을 이용한 산림탄소저장량 추정 방법 비교 연구)

  • Kim, Kyoung-Min;Lee, Jung-Bin;Jung, Jaehoon
    • Korean Journal of Remote Sensing
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    • v.31 no.5
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    • pp.449-459
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    • 2015
  • The conventional National Forest Inventory(NFI)-based forest carbon stock estimation method is suitable for national-scale estimation, but is not for regional-scale estimation due to the lack of NFI plots. In this study, for the purpose of regional-scale carbon stock estimation, we created grid-based forest carbon stock maps using spatial ancillary data and two types of up-scaling methods. Chungnam province was chosen to represent the study area and for which the $5^{th}$ NFI (2006~2009) data was collected. The first method (method 1) selects forest type map as ancillary data and uses regression model for forest carbon stock estimation, whereas the second method (method 2) uses satellite imagery and k-Nearest Neighbor(k-NN) algorithm. Additionally, in order to consider uncertainty effects, the final AGB carbon stock maps were generated by performing 200 iterative processes with Monte Carlo simulation. As a result, compared to the NFI-based estimation(21,136,911 tonC), the total carbon stock was over-estimated by method 1(22,948,151 tonC), but was under-estimated by method 2(19,750,315 tonC). In the paired T-test with 186 independent data, the average carbon stock estimation by the NFI-based method was statistically different from method2(p<0.01), but was not different from method1(p>0.01). In particular, by means of Monte Carlo simulation, it was found that the smoothing effect of k-NN algorithm and mis-registration error between NFI plots and satellite image can lead to large uncertainty in carbon stock estimation. Although method 1 was found suitable for carbon stock estimation of forest stands that feature heterogeneous trees in Korea, satellite-based method is still in demand to provide periodic estimates of un-investigated, large forest area. In these respects, future work will focus on spatial and temporal extent of study area and robust carbon stock estimation with various satellite images and estimation methods.

Arsenic Speciation and Risk Assessment of Miscellaneous Cereals by HPLC-ICP-MS (HPLC-ICP-MS를 활용한 잡곡의 비소 화학종 및 위해 분석)

  • An, Jae-Min;Hong, Kyong-Suk;Kim, Sung-Youn;Kim, Dae-Jung;Lee, Ho-Jin;Shin, Hee-Chang
    • Korean Journal of Environmental Agriculture
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    • v.36 no.2
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    • pp.119-128
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    • 2017
  • BACKGROUND: Miscellaneous cereal have been largely consumed in Korea as due to their physiological functions beneficial to human health. The cereals are currently a social concern because they have been found to contain heavy metals. Thus, monitoring heavy metals in the cereals is an important requirement for food safety analysis. In this study, we determined arsenic concentration in the cereals randomly harvested from different markets. METHODS AND RESULTS: Inorganic arsenic was determined by ICP-MS coupled with HPLC system. The HPLC-ICP-MS analysis was optimized based on the limit of detection and recover test to reach $0.13-1.24{\mu}g/kg$ and 94.3-102.1%, respectively. The concentrations of inorganic arsenic equivalent to daily exposure were levels of $19.91{\mu}g/day$ in mixed grain, $1.07{\mu}g/day$ in glutinous rice, $0.77{\mu}g/day$ in black brown rice, $0.13{\mu}g/day$ in barley and $0.11{\mu}g/day$ in soybeans. CONCLUSION: The levels of arsenic in miscellaneous cereals were found lower than the recommended The Joint FAO/WHO Expert Committee on Food Additives (JECFA) levels, suggesting that the cereals marketed in Korea are not potential concern in risk assessment.

Improvement of Analytical Method for Total Polysaccharides in Aloe vera Gel (알로에 베라(Aloe vera) 겔 중 총 다당체 시험법 개선)

  • Lee, Young-Joo;Kim, Yun-Je;Leem, Dong-Gil;Yoon, Tae-Hyung;Shin, Ji-Eun;Yoon, Chang-Yong;Kim, Jung-Hoon;Park, Mi-Sun;Kang, Tae-Seok;Jeong, Ja-Young
    • Journal of Food Hygiene and Safety
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    • v.27 no.3
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    • pp.271-276
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    • 2012
  • This study intented to standardize the method for total polysaccharide, which is a functional marker for aloe vera gel in Korea. We used four lyophilized raw materials and commercial aloe gel products, certified as Health Functional Food by Korea Food and Drug Administration, including powder, solution, jelly, tablet and capsule, to optimize the analytical condition of dialysis and phenol-sulfuric acid reaction in polysaccharide analysis. The optimal conditions for polysaccharide analysis included 1 L water for dialysis and change 3 times for 24hr against 25 mL prepared sample solution. Validation test showed lower than 5% of coefficient of variation(CV) in intra-, interday validation in lyophilized raw materials and 4 types of commercial products. In inter-person and inter-laboratory validation with 4 persons from 4 different laboratories, CV(%) were 5.50 and 6.64 respectively. The linearity of polysaccharide analysis was assessed using 5 serial concentration of lyophilized raw materials(0.1, 0.2, 0.3, 0.4, 0.5%(w/v)). The results showed $R^2{\geq}0.995$ of high linearity. In the commercial aloe vera gel products, the results of reproductivity showed lower than 7.08% and revealed that the standardized method from this study ensured high precision for polysaccharide analysis.

The Effects of Learning Transfer on Perceived Usefulness and Perceived Ease of Use in Enterprise e-Learning - Focused on Mediating Effects of Self-Efficacy and Work Environment - (지각된 유용성과 사용용이성이 기업 이러닝 교육의 학습전이에 미치는 영향에 관한 연구 -자기효능감과 업무환경의 매개효과를 중심으로-)

  • Park, Dae-Bum;Gu, Ja-Won
    • Management & Information Systems Review
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    • v.37 no.3
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    • pp.1-25
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    • 2018
  • This research performed the empirical test for the effects of learning transfer on perceived usefulness, perceived ease of use, self-efficacy and work environment using 390 employees who have experienced e-learning in domestic and foreign companies. Analyzed the mediating effects of self-efficacy and work environment in addition to direct effect of each factor on learning transfer. The results showed that perceived usefulness and perceived ease-of-use of e-learning learner had a positive(+) effect on self-efficacy and a positive influence on supervisor and peer support and organizational climate. Self-efficacy showed a positive effect on learning transfer, and supervisor support, peer support and organizational climate had a positive influence on learning transfer as well. Perceived usefulness also had a positive effect on learning transfer. However, perceived ease-of-use had no significant effect on learning transfer. As a result of the mediating effect analysis, self-efficacy and work environment were analyzed to have mediating effects between perceived usefulness, perceived ease of use, and learning transfer. The implications of this study are as follows. First, this study designed a new research model that reflects factors influencing the effect of learning transfer on acceptance of e-learning that is common in corporate education. It has derived a research model of perceived usefulness and perceived ease-of-use, which were used as mediating variables for external characteristics factors, as independent variables, using self-efficacy and work environment as mediating variables, which were studied as external factors. Second, most of the studies on technology acceptance model and learning transfer are conducted in a single country. The reliability was enhanced by testing the study models using different samples from 26 countries. Third, perceived usefulness and ease-of-use in existing studies have been considered as key determinants of acceptance intention and learning transfer. This study explored the mediating effects of learner and environmental factors on the accepted information technology and strengthened and supplemented the path of learning transfer of perceived usefulness and ease-of-use. In addition, based on the sample analysis of various countries used in this study, it is expected that future international comparative studies will be possible.

Assessment Research Comparing the Environmental Value of Taebaeksan·NakSan·Kyeongpo Provincial Parks of Kangwon-do (태백산, 낙산, 경포도립공원의 환경가치비교 평가연구)

  • Kang, Kee-Rae;Kim, Dong-Pil;Cho, Woo;Baek, Jae-Bong
    • Korean Journal of Environment and Ecology
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    • v.30 no.2
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    • pp.253-260
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    • 2016
  • This research aimed to quantitatively measure the environmental values of Taebaeksan, Naksan, and Gyeongpo provincial parks located in Gangwon-do. The research was based on the CVM technique which estimates the economic values for all kinds of ecosystem. Also, the estimated value of environment goods can suggest the magnitude of additional utility other than the cost people pay when they visit the provincial parks. Such result can be used as basic data in addition to information on natural ecology or cultural landscape to decide whether the park should be promoted as a national park. The questionnaires-collected from Taebaeksan(180 copies), Naksan(179 copies), and Gyeongpo(180 copies) provincial parks were used to measure the environmental value of each provincial park. Variables that affect the response of 'yes(Y)' or 'no(N)' to the cost suggestion for the economic valuation of environment are estimated under the catagories of environment conservation status (env.), degree of park management (manage.), environmental conservation effort, education (edu.), and income (inc.) of the respondents (execu.), pertaining to the 3 provincial parks in Gangwon-do. The value of natural environment to 1 visitor to the 3 Gangwon provincial parks was estimated by the Logit method that Hanemann proposed using the average of inserted variables. The results showed that the additional environmental value that 1 visitor can gain is 44,060 won for Taebaeksan Provincial Park, 41,191 won for Naksan, and 41,844 won for the Gyeongpo Provincial Park. Taebaeksan Provincial Park's environmental value is estimated at the highest as the respondents judge that its natural environment is well preserved and the facilities are managed well.

A Study on Contents of Vitamin K1 in Local Agricultural Products (지역농산물의 비타민 K1 함량 조사)

  • Lee, A Reum;Kim, Joo Hee;Park, Jae-Ho;Kim, Youngho;Hong, Eui Yon;Kim, Haeng-Ran;Choi, Youngmin;Lee, Junsoo;Eom, Hyun-Ju
    • The Korean Journal of Food And Nutrition
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    • v.29 no.3
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    • pp.301-306
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    • 2016
  • Vitamin K (phylloquinone) is an essential cofactor in the synthesis of active blood-clotting factors II, VII, IX and X. Deficiency of vitamin K leads to inadequate activity of these factors, resulting in bleeding. In this study, we investigated vitamin $K_1$ content of agricultural products that are widely and specifically grown in Korea including 9 leaves and vegetables, 16 fruits, and 11 cereals and specialty crops. Vitamin $K_1$ analysis of the agro-samples was by a validated, modified, reversed phase-HPLC method with fluorescence detection after post-column derivatization. The vitamin $K_1$ content ranged from 1.83 to $682.73{\mu}g$/100 g in leaves and vegetables, 0.17 to $28.22{\mu}g$/100 g in fruits, and ND to $279{\mu}g$/100 g in cereals and specialty crops. Among the 36 samples, high content of vitamin $K_1$ were found in Gugija (Lycium chinense Miller) leaves (average $682.73{\mu}g$/100 g) and Hansan ramie leaves (average $423.12{\mu}g$/100 g); however, mushroom, amaranth and Chinese artichoke showed no detectable levels. The results of ourstudy provide reliable vitamin $K_1$ content of Korean grown agricultural products that expand nutritional information and food composition database.