• Journal of Ginseng Research
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• v.27 no.3
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• pp.146-150
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• 2003

This study was carried out to obtain basic information on breeding using PCR-aided RFLP technology which can identify the variation inter- and intra-species of ginseng in the level of DNA. It was intended to investigate banding pattern on psbA and rbeL genes of chloroplast DNA in ginseng after treating with restriction enzymes. To isolate psbA and rbcL genes of chloroplast, both psbA-N, psbA-C primer and rbcL-N, PX-1 primer were used. As a result, 1,008 bp band of psbA gene and 1,336 bp band of rbcL gene were appeared, which was optimal and expected molecular weight. In addition, primers to isolate atpB, rpoB, trnL, and trnF genes were used, resulting in the expected 1366, 900, 1500 and 1008 bp bands. Genes of psbA and rbcL isolated by PCR were cut by restriction enzymes, Sau3A, TaqI, AluI, HaeIII, and RFLP pattern was investigated. KG line and other species of ginseng were cut by TaqI treatment, and bands were located in 800 bp. The treatment treated by AluI also showed the same 800 bp band in KG line and other species. In HaeIII treatment, 500 bp of faint bands were shown in case of KG line, whereas any bands were not observed in other species. All chloroplast genes formed bands by PCR amplification. However, it was not evident to distinguish intra-or inter-species of ginseng after restriction enzyme treatment. Therefore, more restriction enzyme treatment or sequence comparison method should be considered for further experiment.

• Korean Journal of Plant Taxonomy
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• v.31 no.3
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• pp.223-251
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• 2001

Morphological characters of section Atratae of the genus Carex (Cyperaceae) were reexamined. The epidermal patterns of perigynium, achene and leaf were investigated using by a scanning electron microscope (SEM) and a light microscope (LM). Morphological characters such as length and width of stem, leaf, bract, spike, scale, perigynium and achene, and shape of cross-sectioned stem, and leaf, spike, scale, apex of scale, perigynium, beak of perigynium and achene, and epidermal pattern of perigynium, achene and leaf (shape of fundamental epidermal cell and cell wall, type of silica body, shape of beak epidermal cell and cell wall in perigynium, subsidiary cell shape, size and frequency of stomatal complex of leaf) were useful for the identifications of the observed seven taxa. C. gmelinii, C. hancokiana and C. peiktusani have been confused each other due to similar morphological characters. Howerever these taxa were distinct with respect to shape of scale, apex of scale, perigynium, type of silica body, frequence of stomatal complex, subsidal cell shape, and epidermal patterns of achene and leaf.

• Journal of Korean Society of Forest Science
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• v.77 no.1
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• pp.53-64
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• 1988

The idiogram of pine chromosomes was obtained from the length, the ratio of the long and short arm, and the position of the secondary constriction. The descending order of the long arm was found by analyzing the idiogram for 6 species of hard pines and 5 species of soft pines growing in Korea. The basic chromosome number of the genus Pinus was n=12, of which the ten chromosomes were the M-type showing similar S/L ratio, and the other two short chromosomes were the heterobrachial SM-type and the sub-median centric SM-type. The interspecific identification was able to made by comparing the number and the position of the secondary constriction, and the pattern of descending order of the long arm. The intraspecific variation was also able to be identified by comparing the long arms Descending order among the provenaces. Some differences were found in the chromosomal structures between the hard- and the soft-pines. However, the differences were not apparent as much as those in the morphological characteristics. The results might not be exactly reproducible because of the variable responses of chromosomes depending on concentration of the chemicals, the temperatures and time of the treatments, and the analytical errors during the preparateur preparation.

• Applied Microscopy
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• v.39 no.2
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• pp.73-80
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• 2009

The plastid inclusion has long been known to exist in leaves of numerous plant species, especially in those of flowering plants. Among the inclusions, crystalline bodies are the most frequently distinguished structures of the foliar plastids, however, microtubules and phytoferritins are also reported occasionally. The crystalline inclusions vary in shape, and are located either in the stroma or within intrathylakoidal spaces, whereas microtubules and phytoferritins are more uniform in shape and are formed in the stroma. In crystalline structures, the composing elements exhibit a lattice pattern and/or paralleled tubules that are either bounded by membranes or exist without membrane enclosing. Other types of inclusions have not been shown to be enclosed by any membranous structures. According to the current survey, the plastid inclusion, with the exception of phytoferritins, has been shown to exhibit a crystalline or tubular pattern, and has been reported in more than 56 species of various families. Their occurrence is not restricted to any photosynthetic pathway, but is found to be randomly distributed among C-3, C-4 and CAM species, without phylogenetic relationships. The progress in plastid inclusion research reveals more information about the function and complexity, but the need for characterizing the 3-D structure of the crystalline inclusions also has been acknowledged in previous studies. A 3-D characterization would utilize tilting and tomography of serial sections with appropriate image processing that would provide valuable information on the sub-structures of the crystalline inclusions. In fact, recent studies performed on 3-D reconstruction of the plastid inclusions revealed important information about their comprising elements. In this article, the crystals and microtubules that have been reported in various types of plastids have been reviewed, with special consideration given to their possible sub-cellular function within the plastids.

• Conservation Science in Museum
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• v.10
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• pp.43-61
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• 2009

The Buyeo National Museum was requested conservation treatment for wooden objects excavated from three Baekje archeological sites: Neungsan-ri, Ssangbuk-ri, and Gungnamji Pond. Prior to conservation treatment, analysis was conducted to identify the species used. The results of the analysis revealed wood from diverse species of trees including Hard pine, Cryptomeria japonica D. Don, Zelkova serrata Makino, Quercus spp., Platycarya strobilaceae S. et Z., Castanea spp., Torreya nucifera S. et Z., Taxus cuspidata S. et Z., and Salix spp. A high percentage of the objects were made of Cryptomeria japonica D. Don., a species native to Japan, which indicates that exchange with Japan was active at that time. Among the wooden objects, we analyzed lacquer fragments from six pieces of lacquerware, and the characteristics of the lacquer fragments were peculiar to specific artifacts. Most of the fragments were thicker than 100 ㎛. Pure lacquer and mixed black pigment were used. Infrared spectroscopy of the lacquered wooden fragments revealed that they had a very similar absorption band as refined lacquer, confirming that they were painted with lacquer. For their conservation, we immersed the objects in a high molecular weight aqueous solution of PEG#3,350 (10% → 50%) to strengthen them before vacuum freeze-drying.

• Korean Journal of Poultry Science
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• v.41 no.3
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• pp.191-196
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• 2014

The identification and utilization of potential candidate genes with significant effects on economically important traits have become increasingly important in poultry breeding programs. The ovocalyxin-32 (OCX-32) gene is located chromosome 9 in chicken, plays an important role in eggshell formation. This study was performed to assess the association between single nucleotide polymorphisms (SNPs) of OCX-32 gene and egg production traits in the Korean native chicken. Four Korean native chicken population (n = 181; including 46 females of Ogol, 46 females of white, 43 females of gray and 46 females of black) were used to analyze two SNPs (c.494A>C and c.267T>G) in the OCX-32 gene by PCR-RFLP (Polymerase Chain Reaction-Restricted Fragment Length Polymorphism). We measured egg production traits of age at first egg, first egg weight, egg production ratio and egg weight. The SNP c.494A>C was significantly associated with egg production ratio in Korean Ogol chickens (p<0.001) and egg weight in Korean white chickens (p<0.05). SNP c.267T>C was significantly associated with egg weight in Korean Ogol chickens (p<0.05). But there was no significant association in Korean gray and black chickens. Results suggest the possibility of using molecular markers in OCX-32 gene as a tool for performance and egg production traits in Korean native chicken breeding program.

• Journal of the Korean Society for information Management
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• v.22 no.3 s.57
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• pp.351-378
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• 2005

The study intended to develop KORMARC for archives in order to integrate archives with library materials. The results of the study can be summarized as follows; (1) 2 areas for conservation and physical description are added to the existing 7 areas of ISAD(G)2. The study has also proved that the existing 26 elements of ISAD(G)2 are not fully enough to satisfy the information demands' of institutions and its users as well. (2) For the use of domestic archives in particular, the study has added the description elements of archives that appeared in the Government Regulations of Office Management and those forms of documents that are specified by law for the sake of computerization. The study has added the possible release and grade, release dates, release range, conservation periods, conservation periods, conservation value, the status description of archives elements that are specified in Public Record Management Law. (3) The study has developed the following data fields to be added into KORMARC. 512 creation dates note, 555 finding aids note, 583 action note and 584 accumulation note. Also it reorganizes and adds the indicators of the 245 title statement, 300 physical description 306 playing time, 506 restriction on access note, 534 original version note, 535 location of originals/duplicates note, 540 terms governing use and reproduction notes, 541 immediate source of acquisition note, 545 biographical or historical note, 581 publication note, 850 holding institution data fields.

• The Journal of the Korea Contents Association
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• v.17 no.12
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• pp.1-10
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• 2017

Open data has received a lot of attention from around the world. The Korean government is also making efforts to open government data. However, despite the quantitative increase in public data, the lack of data is still pointed out. This paper proposes a method to improve data sharing and utilization by semantically linking public data. First, we propose a knowledge model for expressing administrative districts and their semantic relationships in Korea. An administrative district is an administrative unit that divides the territory of a nation, which is a unit of politics, according to the purpose of the state administration. The knowledge model of the administrative district defines the structure of the administrative district system and the relationship between administrative units based on the Local Autonomy Act. Second, a knowledge graph of the administrative districts is introduced. As a reference information to link public open data at a semantic level, some characteristics of a knowledge graph of administrative districts and methods for linking heterogeneous public open data and improving data quality are addressed. Finally, some use cases are addressed for interlinking between the knowledge graph of the administrative districts and public open data. In particular, national administrative organisations are interlinked with the knowledge graph, and it demonstrates how the knowledge graph can be utilised for improving data identification and data quality.

• Journal of Korean Library and Information Science Society
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• v.47 no.1
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• pp.217-242
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• 2016

To investigate the characteristics of Korean researchers who have published journal articles in the international Library and Information Science (LIS) journals, we analyzed the types of institutions of the authors and their disciplines by publication year and journal type. Specifically, we analyzed 819 pieces of author information from 384 articles published between 2005 and 2014 in 31 journals using the existing categories, allowing comparisons of research trends between past and current and between Korean and international researchers. In addition, we used a co-authorship credit allocation method to calculate the credits of individual authors in an accurate manner, and found that 342.6 of 384 papers were contributed by solely Korean researchers. We demonstrated that the internationality and quality of Korean LIS research has increased during this time. In particular, authors who were affiliated with universities took the lead in publishing papers, and we presented that the nature of research might be related to the type of author affiliation. Based on the disciplinary backgrounds of the researchers, we also suggest that LIS research is associated with such disciplines, computer science, management, and communications.

• The KIPS Transactions:PartB
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• v.9B no.2
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• pp.245-254
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• 2002

IC package marking inspection system using machine vision locates and identifies the target elements from input image, and decides the quality of marking by comparing the extracted target elements with the standard patterns. This paper proposes an adaptive multi-level thresholding (AMLT) method which is suitable for a series of operations such as locating the target IC package, extracting the characters, and detecting the Pinl dimple. It also proposes a dynamic matching unit selection (DMUS) method which is robust to noises as well as effective to catch out the local marking errors. The main idea of the AMLT method is to restrict the inputs of Otsu's thresholding algorithm within a specified area and a partial range of gray values. Doing so, it can adapt to the specific domain. The DMUS method dynamically selects the matching unit according to the result of character extraction and layout analysis. Therefore, in spite of the various erroneous situation occurred in the process of character extraction and layout analysis, it can select minimal matching unit in any environment. In an experiment with 280 IC package images of eight types, the correct extracting rate of IC package and Pinl dimple was 100% and the correct decision rate of marking quality was 98.8%. This result shows that the proposed methods are effective to IC package marking inspection.