• Korean Journal of Environmental Biology
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• v.29 no.1
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• pp.68-73
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• 2011

The single cell gel electrophoresis (SCGE) assay is a microelectrophoretic technique for assessments of DNA damage at the level of the individual eukaryotic cell. The SCGE assay, due to its simplicity, sensitivity and need of a few cells, has advantages compared to other genomic damage assays such as sister chromatid exchange, chromosomal aberration and micronucleus test. In this study, investigated were the levels of DNA damage and the repair kinetics in the coelomocytes of Eisenia fetida treated with HgCl2 and ionizing radiation by means of the SCGE assay. For detecting DNA damage and repair in coelomocytes, earthworms (E. fetida) were irradiated with six doses of ${\gamma}$-rays (0, 2.5, 5, 10, 20 and 50 Gy) and in vivo exposed to mercuric chloride at 0, 80 and 160 mg $kg^{-1}$ for 48 hours. Then the Olive tail moments were measured during 0~12 hours after irradiation and 0~72 hours after Hg treatment. The results showed that the more the oxidative stress was induced by mercury and radiation, the longer the repair time was required. Also, the results suggest that the SCGE assay may be used as an important tool for comparison of the sensitivity of different species to oxidative stresses.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.20 no.2
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• pp.75-80
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• 2006

In this paper, to detect and observation the void discharges pulse signal, AE signals and tree growth characteristics in case the high voltage is applied to a XLPE sample for a power cable. We also examined the partial discharge current pulse and AE signals with the increase of the applied voltage in XLPE insulation. The experimental results show that a branch-type tree grows in the presence of the voids, and a bush-type tree grows in the absence of the voids in both samples. A rate of tree growth increases abruptly in proportional to the deterioration time in the presence of the of the voids, but in the absence of the voids, a rate of tree growth decreases as time goes by and finally a breakdown occurs. The frequency band of AE signals that are generated from the partial discharges in a XLPE sample, one of solid dielectric materials, is about 1.0[MHz].

• The Journal of the Korea Contents Association
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• v.5 no.5
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• pp.296-304
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• 2005

Quantitative analysis compare to dynamic characteristic change of the regional cerebral blood volume(rCBV) after development of cerebral fat embolism in cats using perfusion magnetic resonance(MR) Imaging. Twenty cats were used. Linoleic acid (n=11) were injected into the internal carotid artery using microcatheter through the transfemoral approach. Polyvinyl alcohol (Ivalon) (n=9) was injected as a control group. Perfusion MR images were obtained at 30 minutes and 2 hours after embolization, based on T2 and diffusion-weighted images. The data was time-to-signal intensity curve and ${\Delta}R_2^*$ curve were obtained continuously with the aid of home-maid image process algorithm and IDL(interactive data Banguage, USA) softwares. The ratios of rCBV increased significantly at 2 hours compared with those of 30 minutes (P<0.005). In conclusion, cerebral blood flow decreased in cerebral fat embolism immediately after embolization and recovered remarkably in time course. It is thought that clinically informations to dynamic characteristic change of the cerebral hemodynamics to the early finding in cerebral infarction by diffusion weighted imaging(DWI) and perfusion weighted imaging(PWI).

• Journal of the Korean Society for information Management
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• v.9 no.1
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• pp.55-82
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• 1992

It can be assumed that exciting improvements of technology concerning the ways in which information is stored and processed have significantly affected information science and may lead to a dramatic change of research in the field. Author co-citation analysis was used to investigate changes in research patterns of information retrieval over two time periods, 1980-1982, and 1988-1990. Nonmetric multidimensional scaling and clustering techiqes were used to create two dimensional maps displaying the changing research patterns among 22 authors as perceived by scholars citing their work over the two time periods. The co-cited author clusters and the placement of authors on the two maps both appear to correspond well with characterizations of the author's work obtained in texts and social relationships.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.373-377
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• 2007

본 연구는 하천수를 직접 취수하는 기존 취수장의 취수장애 문제를 해결하기 위한 대안을 개발하는데 목적이 있다. 이를 위해 지금까지 취수장 문제해결 방법으로 검토되지 않았던 유로(流路) 및 하천의 지형변동을 GIS 및 항공사진을 이용하여 정량적으로 파악하였으며, 유사영역 구분 및 하도특성 분석, 하천 특성 해석에 적합한 이동경계 좌표계를 이용한 수치모형을 적용하여 2차원 하상 및 지형변동 예측을 검토하였다. 저수로의 이동 및 하상저하로 인하여 취수문제가 발생하고 있는 경상북도 구미시에 위치한 해평취수장 주변에 대하여 항공사진 분석 및 GIS 기법을 이용하여, 시간 경과에 따른 하천 형태 변화 저수로의 이동 특성을 조사하고 분석하였다. 저수로는 좌안에서 우안으로 이동해 가고 있으며, 이는 하천의 경사가 급하고 하폭이 증가하면서 발생하는 현상으로 저수로의 불안정성이 크게 증가 하는 특성을 보여주었다. 시간의 증가에 따라 저수로 하폭의 증가율이 감소하고 있으며, 수로의 측방 이동을 보여주고 있으며, 시간이 증가함에 따라 측방향 이동율이 감소하였다. 만재유량을 이용하여 유사의 영역구분을 수행한 결과, 망상하천으로서, 모래하천으로 부유사가 지배적인 하천으로 판단되었다. 2차원 수치모형을 이용하여 하상변동을 모의한 결과, 유량이 $2,000\;m^3/s$일 경우에는, 해평 취수장 측의 하천 하상은 상승하였으며, 구미정수장 측에서는 하상이 저하되었다. 홍수유량 $8,000\;m^3/s$일 경우에는 해평 취수장 측에서는 약간 저하하였고, 구미정수장 측에서는 하상이 상승하나, 직하류에서는 하상이 세굴되고, 하천 가운데 자리하고 있는 하중도는 커지는 현상을 보여 주었다. 또한 하중도 직하류에서는 하중도를 중심으로 좌안인 해평 취수장과 우안인 구미정수장 쪽으로 분류된 흐름이 합류되면서 하상에서는 퇴적과 세굴이 반복되며, 복렬 사주가 발생하는 것으로 나타났다. 이는 두 흐름에 의해 와(vortex)가 크게 형성되어 하상의 세굴에 영향을 미치기 때문으로 판단되었다.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.1A
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• pp.53-59
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• 2010

Recently, NDTs (Non-Destructive Techniques) using infrared camera are widely studied for detection of damage and void in RC (reinforced concrete) structures and they are also considered as an effective techniques for maintenance of infrastructures. The temperature on concrete surface depends on material and thermal properties such as specific heat, thermal conductivity, and thermal diffusion coefficient. Different porosity on cement mortar due to different mixture proportions can show different heat behavior in cooling stage. The porosity can affect physical and durability properties like strength and chloride diffusion coefficient as well. In this paper, active thermography which uses flash for heat induction is utilized and thermal characteristics on surface are evaluated. Samples of cement mortar with W/C (water to cement ratio) of 0.55 and 0.65 are prepared and physical properties like porosity, compressive strength, and chloride diffusion coefficient are evaluated. Then infrared thermography technique is carried out in a constant room condition (temperature $20{\sim}22^{\circ}C$ and relative humidity 55-60%). The mortar samples with higher porosity shows higher residual temperature at the cooling stage and also shows reduced critical time which shows constant temperature due to back wall effect. Furthermore, simple equation for critical time of back wall effect is suggested with porosity and experimental constants. These characteristics indicate the applicability of infrared thermography as an NDT for quality assessment of cement based composite like concrete. Physical properties and thermal behavior in cement mortar with different porosity are analyzed in discussed in this paper.

• The Journal of Engineering Geology
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• v.23 no.4
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• pp.409-425
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• 2013

This study presents a prediction of a time-series of the area inundated by effluent from Heavenly Lake caused by ground behavior prior to a volcanic eruption. A GIS-based hydrological algorithm that considers the multi-flow direction of effluent, the absorption and storage capacity of the ground soil, the storage volume of the basin or the depression terrain, was developed. To analyze the propagation pattern, four hypothetical collapse zones on the cheonji ground were set, considering the topographical characteristics and distributions of volcanic rocks at Mt. Baekdu. The results indicate that at 3 hours after collapse, for both scenarios 1 and 2 (collapses of the entire/southern boundary of cheonji), a flood hazard exists for villages in China, but not for those on the North Korean side of the mountain, due to the topographical characteristics of Mt. Baekdu. It is predicted that villages in both North Korea and China would be significantly damaged by flood inundation at 3 hours elapsed time for both scenarios 3 and 4 (collapses on the southern boundary of cheonji and on the southeastern-peak area).

• Radiation Oncology Journal
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• v.19 no.2
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• pp.163-170
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• 2001

Purpose : The measurement of radiation survival using a clonogenic assay, the established standard, can be difficult and time consuming. In this study, We have used the MTT assay, based on the reduction of a tetrazolium salt to a purple formazan precipitate by living cells, as a substitution for clonogenic assay and have examined the optimal condition for performing this assay in determination of radiation sensitivity. Materials and Methods : Four human cancer cell lines - PCI-1, SNU-1066, NCI-H630 and RKO cells have been used. For each cell line, a clonogenic assay and a MTT assay using Premix WST-1 solution, which is one of the tetrazolium salts and does not require washing or solubilization of the precipitate were carried out after irradiation of 0, 2, 4, 6, 8, 10 Gy. For clonogenic assay, cells in $25\;cm^2$ flasks were irradiated after overnight incubation and the resultant colonies containing more than 50 cells were scored after culturing the cells for $10\~14$ days. For MTT assay, the relationship between absorbance and cell number, optimal seeding cell number, and optimal timing of assay was determined. Then, MTT assay was performed when the irradiated cells had regained exponential growth or when the non-irradiated cells had undergone four or more doubling times. Results : There was minimal variation in the values gained from these two methods with the standard deviation generally less than $5\%$, and there were no statistically significant differences between two methods according to t-test in low radiation dose (below 6 Gy). The regression analyses showed high linear correlation with the $R^2$ value of $0.975\~0.992$ between data from the two different methods. The optimal cell numbers for MTT assay were found to be dependent on plating efficiency of used cell line. Less than 300 cells/well were appropriate for cells with high plating efficiency (more than $30\%$). For cells with low plating efficiency (less than $30\%$), 500 cells/well or more were appropriate for assay. The optimal time for MTT assay was after 6 doubling times for the results compatible with those of clonogenic assay, at least after 4 doubling times was required for valid results. In consideration of practical limits of assay (12 days, in this study) cells with doubling time more than 3 days were inappropriate for application. Conclusion : In conclusion, it is found that MTT assay can successfully replace clonogenic assay of tested cancer cell lines after irradiation only if MTT assay was undertaken with optimal assay conditions that included plating efficiency of each cell line and doubling time at least.

• Investigative Magnetic Resonance Imaging
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• v.4 no.2
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• pp.100-106
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• 2000

Purpose : To evaluate the usefulness of cerebral blood flow measurement applied to perfusion weighted image with short-scan time single shot gradient echo-planar technique in measuring cerebral blood volume(rCBV) of normal rabbits. Materials and methods : With 2.1-3.6 kg weighted rabbits, image is acquired when they are in supine position in children positioner. Perfusion weighted image is acquired to 44 seconds per 1 second successively. After 4 seconds later, Gd-DTPA 2ml are injected into int. jugular vein with 2 ml per second and normal saline is also injected after that. Same technique is applied 2 times per 30 minites in same rabbit. After Image is obtained in two part of cerebral cortex at vertex, convexity, in one of basal ganglia with choosing about $3-5{\textrm{mm}^2}$ areas. Curve of signal intensity changes in time sequence is drawn. After this images are transmitted by PC and software IDL, regional cerebral blood volume is measured with imaging processing program made by us. Results : With 22 of 24 rabbits, satisfactory 1-2 signal intensity versus time curve is made. Cerebral blood capacity and contrast media stay time (ST) is measured in two cerebral cortex and basal ganglia refering in parietal cerebral cortex. Mean focal cerebral blood flow capacity ratio in cortex was $0.97{\pm}0.35$ and in basal ganglia, $0.99{\pm}0.37$, mean contrast media stay time in cortex was $9.83{\pm}1.63$ sec and in basal gaiglia, $9.42{\pm}1.14$ sec, but there was no statistically significant difference between two areas ($\rho$=0.05). Conclusion : In cerebral cortex and basal ganglia, there is no difference in mean focal blood volume and mean contrast stay time. Therefore, PWI is useful in cerebral blood flow and early diagnosis, prognosis of cerebral ischemic disease. Hereafter, it is helpful in analysing cerebral blood flow changes with comparison difference in rCBV between normal tissue and ischemic tissue, and that with DWI finding in infarcted patient.

• Proceedings of the Korea Technology Innovation Society Conference
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• 2004.11a
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• pp.101-113
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• 2004

본 연구는 기술혁신파급경로를 결정하는 국가 간 무역역할에 대한 실증적인 분석을 목적으로 한다 이 연구목적을 위하여, 최근 자료인 1980년부터 2003년까지 15개 OECD국가를 대상으로 자국의 기술혁신을 결정하는 중요한 변수로 알려진 자국 R&D축적 및 무역대상국의 R&D축적자료를 구축하였으며, 이를 무역지수인 쌍방간에 수출 및 수입량을 경제규모로 나눈 가중지수를 이용하여 유입된 R&D축적량을 구축하였다. 또한 대상변수들의 기술혁신파급역할에 대하여 최근 논의되고 있는 비정상적 패널기법을 이용하여 분석하였다. 최근 제안되고 있는 비정상적 패널기법을 이용하여 국제 간에 기술혁신파급경로를 분석한 결과를 간단하게 요약하면, 다음과 같다. 첫째, 분석대상변수들은 비정상성을 갖는 것으로 나타났다. 둘째, 그러나 장기적으로 분석대상변수들이 서로 균형상태를 나타내는 공적분관계에 있음을 알 수 있었다. 셋째, 국가 간에 기술혁신파급경로의 방향과 정도를 파악하기 위하여 패널 공적분계수를 추정하였으나, 설정함수형태에 따라서 여러 가지 상반된 실증결과가 나타났다. 따라서 기존 연구Coe et al., 1995, Keller, 1998, Kao, et al., 1999 그리고 Funk, 2001]의 분석결과 및 그 시사점들이 서로 다른 이유는 분석대상변수들의 선택차이뿐만 아니라, 기술혁신경로에 대한 설정함수형태에 따라서 서로 다른 분석결과가 나타날 수 있는 가능성을 보여준다. 본 연구에서 나타난 분석결과의 시사점을 보면, 국가 간에 기술혁신파급경로분석은 기술혁신파급을 결정하는 매개변수선정도 중요하지만, 결정된 설명변수들 사이에 어떤 기술혁신파급에 관한 연관관계가 존재하는지에 대한 실증분석 즉 파급경로분석도 매우 중요함을 보여준다. 이러한 파급경로분석에는 기존의 선형가정뿐만 아니라 비선형가정을 이용한 기술파급경로분석을 통한 시사점제안이 요구된다.관적인 시스템을 제공하는 것이다.가 생성된다. $M_{C}$에 CaC $l_2$를 첨가한 경우 $M_{C}$는 완전히 $M_{Cl}$ 로 전이를 하였다. $M_{Cl}$ 에 CaC $l_2$를 첨가하였을 경우에는 아무런 수화물의 변화는 발생하지 않았다. 따라서 CaS $O_4$.2$H_2O$를 CaC $O_3$및 CaC $l_2$와 반응시켰을 때의 AFm상의 안정성 순서는 $M_{S}$ < $M_{C}$< $M_{Cl}$ 로 된다.phy. Finally, Regional Development and Regional Environmental Problems were highly correlated with accommodators.젼 공정을 거쳐 제조된다는 점을 고려할 때 이용가능한 에너지 함량계산에 직접 활용될 수는 없을 것이다.총단백질 및 AST에서 시간경과에 따른 삼투압 조절 능력에 문제가 있는 것으로 보여진다.c}C$에서 5시간 가열조리 후 잔존율은 각각 84.7% 및 73.3%였고, 질소가스 통기하에서는 잔존율이 88.9% 및 81.8%로 더욱 안정하였다.8% 및 12.44%, 201일 이상의 경우 13.17% 및 11.30%로 201일 이상의 유기의 경우에만 대조구와 삭제 구간에 유의적인(p<0.05) 차이를 나타내었다.는 담수(淡水)에서 10%o의 해수(海水)로 이주된지 14일(日) 이후에 신장(腎臟)에서 수축된 것으로 나타났다. 30%o의 해수(海水)에 적응(適應)된 틸라피아의 평균 신사구체(腎絲球體)의 면적은 담수(淡水)에 적응된 개체의 면적보다 유의성