• Title/Summary/Keyword: 스펙트럼 분리법

Search Result 101, Processing Time 0.023 seconds

Simultaneous Determination of Fluorene and Anthracene by Synchronous Spectrofluoremetry (동시화 형광분광법에 의한 fluorene과 anthracene의 동시정량)

  • Lee, Sang Hak;Han, Yung Ji;Son, Bum Mok;Choi, Jong Ha;Kim, Chang Jin
    • Analytical Science and Technology
    • /
    • v.15 no.5
    • /
    • pp.393-398
    • /
    • 2002
  • A method to determine simultaneously fluorene and anthracene in aqueous solution by synchronous fluorescence spectrometry has been studied. The emission characteristics and optimum wavelength interval (${\Delta}{\lambda}$) for synchronous spectra of fluorene and anthracene in aqueous solution were investigated. The optimum wavelength interval (${\Delta}{\lambda}$) was found to be 50 nm. The calibration curve for fluorene and anthracene in the synthetic mixture solution of both compounds was linear over the range from $5.0{\times}10^{-8}M$ to $1.0{\times}10^{-3}M$ and from $5.0{\times}10^{-8}M$ to $1.0{\times}10^{-3}M$ for fluorene and anthracene, respectively. The detection limit was $3.0{\times}10^{-9}M$ and $7.0{\times}10^{-9}M$, for fluorene and anthracene, respectively under the optimal wavelength interval.

Molecular detection of blaVIM, blaBIC, blaKPC, and blaSIM genes from isolated bacteria in retail meats (육류용 고기로부터 분자진단을 이용한 항생제내성 유전자 양상)

  • Hwang, You Jin
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.22 no.6
    • /
    • pp.413-419
    • /
    • 2021
  • The purpose of this study was to investigate the ability to treat and prevent infection by multiple Gram-negative bacterial pathogens as a last choice option in the treatment of serious infections in clinical settings. The global spread of extended-spectrum 𝛽-lactamases (ESBLs) and/or carbapenemases in microorganisms are of enormous concern to health services because they are often associated with multi-drug resistance which significantly restricts the antibiotic treatment options. In this study, the antimicrobial resistance profiles of bacteria isolated from South Korean market-derived meat samples were determined by the disc diffusion method. PCR was used to detect the presence of antibiotic resistance genes and ESBL producing genes. In total, we tested 181 isolated colonies from 36 market-derived meat samples. Single PCR and DNA sequencing results revealed that genes blaVIM, blaBIC, blaKPC, and blaSIM were present in the bacteria isolated from retail meat. The bacteria in the meat were separately sequenced and based on alignment, four different bacteria were identified. These findings suggest that bacteria found in retail meats are a reservoir for the spreading of ESBL blaVIM, blaBIC, blaKPC, and blaSIM resistance genes and bacteria strains.

Performance Evaluation of Attention-inattetion Classifiers using Non-linear Recurrence Pattern and Spectrum Analysis (비선형 반복 패턴과 스펙트럼 분석을 이용한 집중-비집중 분류기의 성능 평가)

  • Lee, Jee-Eun;Yoo, Sun-Kook;Lee, Byung-Chae
    • Science of Emotion and Sensibility
    • /
    • v.16 no.3
    • /
    • pp.409-416
    • /
    • 2013
  • Attention is one of important cognitive functions in human affecting on the selectional concentration of relevant events and ignorance of irrelevant events. The discrimination of attentional and inattentional status is the first step to manage human's attentional capability using computer assisted device. In this paper, we newly combine the non-linear recurrence pattern analysis and spectrum analysis to effectively extract features(total number of 13) from the electroencephalographic signal used in the input to classifiers. The performance of diverse types of attention-inattention classifiers, including supporting vector machine, back-propagation algorithm, linear discrimination, gradient decent, and logistic regression classifiers were evaluated. Among them, the support vector machine classifier shows the best performance with the classification accuracy of 81 %. The use of spectral band feature set alone(accuracy of 76 %) shows better performance than that of non-linear recurrence pattern feature set alone(accuracy of 67 %). The support vector machine classifier with hybrid combination of non-linear and spectral analysis can be used in later designing attention-related devices.

  • PDF

Isolation and Purification of Berberine in Cortex Phellodendri by Centrifugal Partition Chromatography (Centrifugal Partition Chromatography에 의한 황백으로부터 Berberine의 분리 및 정제)

  • Kim, Jung-Bae;Bang, Byung-Ho
    • The Korean Journal of Food And Nutrition
    • /
    • v.27 no.3
    • /
    • pp.532-537
    • /
    • 2014
  • Cortex Phellodendri (CP) is derived from the dried bark of Phellodendron amurense. It has been widely used as a drug in traditional Korea medicine for treating diarrhea, jaundice, swelling pains in the knees and feet, urinary tract infections, and infections of the body surface. Many analytical methods have been used to study oriental herbal medicines, such as thin-layer chromatography, column liquid chromatography, and high performance liquid chromatography (HPLC). In this study, preparative centrifugal partition chromatography (CPC) was successfully carried out in order to separate pure compounds from a CP methanol extract. The optimum two-phase CPC solvent system was composed of n-butanol: acetic acid: water (4:1:5 v/v/v). The flow rate of the mobile phase was 3 mL/min in ascending mode with rotation at 1,000 rpm. The CPC-separated fraction and purification procedures were carried out by preparatory HPLC. The $^1H$ NMR spectrum revealed that the resonances at ${\delta}$ 4.10 and 4.20 ppm corresponded to three protons ($-OCH_3$), whereas those at ${\delta}$ 6.10 ppm corresponded to two protons ($-OCH_2O-$). Further, two aromatic protons (H-11 and H-12) conveys a doublet-doublet pattern. The H-11 doublet and H-12 doublet appear at ${\delta}$ 7.98 and 8.11, respectively. The $^{13}C$ NMR. spectrum showed a tetrasubstituted with a methylenedioxy group at C2 and C3, and two methoxy groups at C9 and C10. The chemical structure of the berberine was identified by $^1H$, $^{13}C$-nuclear magnetic resonance and electrospray ionization-mass spectroscopy spectral data analysis.

Isolation and Characterization of Aquatic Humic Acid and Fulvic Acid (수질계의 Humic Acid와 Fulvic Acid의 분리 및 특성)

  • Rhee, Dong Seok
    • Analytical Science and Technology
    • /
    • v.15 no.1
    • /
    • pp.36-42
    • /
    • 2002
  • The dissolved organic carbon extracted from groundwater is separated into humic acid and fulvic acid. They are characterized for their chemical composition, spectroscopic characteristics using UV/VIS, IR and solid state $^{13}C-NMR$ spectroscopy, proton exchange capacity and molecular size distribution. The results are comparable with the literature data. The study explains that the aquatic humic and fulvic acid in this experiment are site-specipic and polydisperse natural organic matter with considerable proton exchange capacity.

An Experimental Analysis of the Contributions to the Radiated Noise due to Panel Vibration of a Rotational Machine (회전체 진동으로 인한 판넬 방사소음의 실험적 기여도 분석)

  • 국형석;허승진;고강호
    • Transactions of the Korean Society for Noise and Vibration Engineering
    • /
    • v.13 no.2
    • /
    • pp.126-131
    • /
    • 2003
  • This study is concerned with the reduction of noise radiation by an industrial fan unit. First, spectral decomposition method is used to decompose the spectrogram obtained in experiments into source function and noise transfer function, and then major noise generation sources are investigated. Among the noise sources involved in the fan unit. this article is focused on the noise source due to vibration of panels of the unit housing. It is shown here that noise radiation associated with the panel vibration can be as significant in some frequency ranges as that associated with other noise sources such as aeroacoustic fan noise.

Material Decomposition through Weighted Image Subtraction in Dual-energy Spectral Mammography with an Energy-resolved Photon-counting Detector using Monte Carlo Simulation (몬테카를로 시뮬레이션을 이용한 광자계수검출기 기반 이중에너지 스펙트럼 유방촬영에서 가중 영상 감산법을 통한 물질분리)

  • Eom, Jisoo;Kang, Sooncheol;Lee, Seungwan
    • Journal of radiological science and technology
    • /
    • v.40 no.3
    • /
    • pp.443-451
    • /
    • 2017
  • Mammography is commonly used for screening early breast cancer. However, mammographic images, which depend on the physical properties of breast components, are limited to provide information about whether a lesion is malignant or benign. Although a dual-energy subtraction technique decomposes a certain material from a mixture, it increases radiation dose and degrades the accuracy of material decomposition. In this study, we simulated a breast phantom using attenuation characteristics, and we proposed a technique to enable the accurate material decomposition by applying weighting factors for the dual-energy mammography based on a photon-counting detector using a Monte Carlo simulation tool. We also evaluated the contrast and noise of simulated breast images for validating the proposed technique. As a result, the contrast for a malignant tumor in the dual-energy weighted subtraction technique was 0.98 and 1.06 times similar than those in the general mammography and dual-energy subtraction techniques, respectively. However the contrast between malignant and benign tumors dramatically increased 13.54 times due to the low contrast of a benign tumor. Therefore, the proposed technique can increase the material decomposition accuracy for malignant tumor and improve the diagnostic accuracy of mammography.

Independent Component Analysis on a Subband Domain for Robust Speech Recognition (음성의 특징 단계에 독립 요소 해석 기법의 효율적 적용을 통한 잡음 음성 인식)

  • Park, Hyeong-Min;Jeong, Ho-Yeong;Lee, Tae-Won;Lee, Su-Yeong
    • Journal of the Institute of Electronics Engineers of Korea CI
    • /
    • v.37 no.6
    • /
    • pp.22-31
    • /
    • 2000
  • In this paper, we propose a method for removing noise components in the feature extraction process for robust speech recognition. This method is based on blind separation using independent component analysis (ICA). Given two noisy speech recordings the algorithm linearly separates speech from the unwanted noise signal. To apply ICA as closely as possible to the feature level for recognition, a new spectral analysis is presented. It modifies the computation of band energies by previously averaging out fast Fourier transform (FFT) points in several divided ranges within one met-scaled band. The simple analysis using sample variances of band energies of speech and noise, and recognition experiments showed its noise robustness. For noisy speech signals recorded in real environments, the proposed method which applies ICA to the new spectral analysis improved the recognition performances to a considerable extent, and was particularly effective for low signal-to-noise ratios (SNRs). This method gives some insights into applying ICA to feature levels and appears useful for robust speech recognition.

  • PDF

Analysis of Effective Optical Pulse Stream Generation using Loop Scheme (루프구조를 이용한 효율적인 광펄스 스트림 발생에 관한 분석)

  • Park, Hyoung-Keun
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.11 no.5
    • /
    • pp.1580-1584
    • /
    • 2010
  • In this paper, we made an analysis of the effective optical pulse stream generation. This is different from conventional approaches, which use fiber or waveguide delay line circuits. The optical pulse multiplication phenomenon occurs when the optical pulse's spectral width is greater than the transfer bandwidth of the coupler used. Therefore, the effective optical pulse stream generation can be controlled by using fiber couplers with different equivalent transfer bandwidths. The pulse separation spacing is controlled by number of cascaded coupler in optical loop mirror coupler scheme.

Pulsed-Delayed Extraction for Resolution Enhancement of Linear Time-of-Flight Mass Spectromenter in Surface-Assisted Laser Desorption/Ionization of Polypropyleneglycol (폴리프로필렌 글리콜의 표면-보조 레이저 탈착/이온화에서 선형 비행시간 질량분석기의 분해능 개선을 위한 시간 지연 추출법의 응용)

  • Kim, Jung Hwan;Kang, Wee Kyung
    • Journal of the Korean Chemical Society
    • /
    • v.44 no.4
    • /
    • pp.328-336
    • /
    • 2000
  • The pulsed-delayed extraction (PDE) in linear time-of-flight mass spectrometer(TOF MS) is characterized on the enhancement of resolution, mass-depth of focus and effect of instrumentahan 2000. The ion signals separate isotopically by up to molecular weight of 2500 in instrumental broadening of 5 ns, which is a good agreement with calculation. The fragmentation paths of PPG can be sug-gested by the isotopica distributions of fragment series produced when PPG desorbed from graphite surface.

  • PDF