• Title/Summary/Keyword: 스퍼민

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Binding Site of Spermine at Poly$[d(A-T)_2]$ and Poly$[d(G-C)_2]$ (Poly$[d(A-T)_2]$, Poly$[d(G-C)_2]$와 스퍼민의 결합 형태에 관한 연구)

  • Yun, Byeong Hwa;Jeon, Sun Hee;Song, Young Dae;Cho, Tae Sub;Kim, Seog K.
    • Journal of the Korean Chemical Society
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    • v.42 no.5
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    • pp.506-511
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    • 1998
  • When the spermine, which is one of the polyamines containing cation in vivo, binds to DNA, it can increase the stability of DNA. At the same time, it can cause B-form to Z-form transformations of DNA. However, because we cannot determine the binding geometry of the spermine to DNA by using spectroscopic methods, nobody can show the accurate binding mechanism of a DNA-spermine complex. Thus, we used DAPI as a spectroscopic probe of spermine, which binding geometry was well known. At the result of base selective binding geometry of spermine to synthetic DNA, the concentration of spermine gets higher, it grows the hydrophobic environment of DAPI which bound the minor groove of adenine-thymine base pair. Simultaneously, spermine seems to bridge the backbones around the minor groove of $poly[d(A-T)_2]$. So that, the intensity of fluorescence spectrum of that shows sudden increasement. In guanine-cytocine base pair, $poly[d(G-C)_2]$, we can suppose that spermine bind to the major groove of that, shoving out the DAPI which is partially intercalated between the base pocket across the major groove of it. In both cases, spermine doesn't show the base selectivity against to DNA.

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Activation of Cabbage Phospholipase D by Polyamines (폴리아민에 의한 양배추 포스포리파제 D의 활성화)

  • Eun-Hie Koh
    • Journal of the Korean Chemical Society
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    • v.47 no.5
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    • pp.466-471
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    • 2003
  • The effect of polyamines on the cabbage phospholipase D(PLD) activity was investigated. The PLD activity was determined by pH-stat titration of phosphatidic acid, one of the enzymatic reaction product, using phosphatidyl choline small unilamellar vesicles as a substrate. The cabbage PLD was activated approximately 4 fold by spermine at 1 mM concentration. This spermine effect appears to be similar to the previous report on the PLD activation of rat brain mitochondrial fraction. It was also found that cationic polypetides such as polylysine and polyhistidine exerted a marked enhancement effect on the cabbage PLD. Particularly polyhistidine exerted approximately 5.5 fold enhancement effect at 0.062 mM concentration. The polyamine effect on the cabbage PLD was reexamined in the phosphatidylcholine/sodium dodecyl sulfate mixed micellar system. The relevance of polyamine effect on PLD activity is discussed in relation to the active site of PLD.

A Key Distribution Protocol Specification using Casper (Casper를 이용한 키 분배 프로토콜 명세)

  • Lee, Sung-Min;Kim, Tai-Yun
    • Annual Conference of KIPS
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    • 2000.04a
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    • pp.1163-1165
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    • 2000
  • 최근 보안 프로토콜의 안전성을 검증하는 연구가 활발히 진행되고 있다. 이러한 연구 중 대표적인 방법이 CSP[1]와 FDR[2]과 같은 정형 기법을 이용하여 프로토콜을 분석하는 것이다. 이 방법은 매우 성공적으로 프로토콜의 문제점을 발견하고 안전성을 검증할 수 있었다. 하지만 CSP를 이용하여 프로토콜을 명세하는 것은 숙달된 전문가에게만 적합하고 시간이 오래 걸린다는 문제점을 갖는다. 이러한 문제점을 해결하여 좀 더 쉽게 프로토콜을 명세하여 CSP 코드를 생성해주는 캐스퍼(Casper)[3]가 제안되었다. 본 논문에서는 세 개의 메시지 교환을 통해서 키를 분배하는 키 분배 프로토콜을 제안하고 캐스퍼를 이용하여 제안한 프로토콜에 대한 정형 명세를 한다. 명세된 스크립트는 캐스퍼를 이용하여 FDR의 입력 파일이 되는 667 라인의 CSP 코드를 생성하였다. 캐스퍼를 이용한 정형 명세는 좀 더 편리하게 프로토콜을 명세할 수 있고 명세 시 발생할 수 있는 에러를 줄임으로써 신뢰성을 높일 수 있다.

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Extrusion of Spur Gear Using High-Energy Ball Milled Al-78Zn Powder (고에너지 볼밀법으로 제조된 Al-78Zn Powder를 이용한 스퍼기어의 압출)

  • Kim, Jin-Woo;Lee, Sang-Jin;Lee, Jung-Min;Kim, Byung-Min
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.33 no.4
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    • pp.440-446
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    • 2009
  • This paper was designed to fabricate the miniature spur gear with pitch circle of 2.25mm using extrusion process of a mechanically alloyed Al-78wt%Zn powder. The mechanical alloying of the powder particles were performed for ball milled times of 4h, 8h, 16 and 32h by the planetary ball milling. The mechanical properties of these alloyed powders, which were compacted and sintered-cylindrical preforms, were estimated using compression test. The results showed that the alloyed powder with average particle size of $10{\mu}m$ milled for 32h has the highest compressive(fractured) strength(288MPa). Extrusions of the miniature spur gear using the alloyed powder were carried out at different extrusion temperatures. Extrusion temperature of $300^{\circ}C$ provided the spur gear with the highest relative density and Vickers hardness and without any surface defects.

Effect of Sperrnine on Phospholipase D Activity in Rat Brain Mitochondrial Preparation (쥐뇌 미토콘드리아 분획에서 포스포리파제 D에 대한 스퍼민의 영향)

  • Koh, Eun Hie
    • Journal of the Korean Chemical Society
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    • v.44 no.5
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    • pp.448-452
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    • 2000
  • Phospholipase D(PLD) is the enzyme catalyzing the hydrolysis of the terminal phosphcester bond of phospholipid head group to produce phosphatidic acid and the corresponding base. The effect of spermine on the PLD activity of rat brain mitochondrial preparation was investigated. Spermine, in the presence of oleic acid, activates the rat brain mitochondrial PLD, whose effect was further enhanced by the presence of divalent cation, $Ca^{2+}$, $Mg^{2+}$, and $Ba^{2+}$. Among the various monoamines tested, only histamine at the high concentration was effective in activation the PLD. Polylysine increased the PLD activity, particularly, the longer chain of the molecule activated the PLD more effectively. There was no significant difference in the substrate specificity for the PLD activity between phosphatidylcholine(PC) and phoshpatidylethanolamine (PE). This substrate specifitiy is different from the PE specificity reported for the intestinal mitochondrial PLD.

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Nanocomposite Electrodes for Methanol Electrooxidation Fabricated by a Sputtering Deposition Method (직접메탄올 연료전지를 위한 박막형 나노복합 전극구조 분석)

  • Ko, A-Ra;Han, Sang-Beom;Song, You-Jung;Lee, Jong-Min;Kim, Jy-Yeon;Lee, Young-Woo;Park, Kyung-Won
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.06a
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    • pp.403-405
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    • 2009
  • RF-스퍼터링법을 사용하여 메탄올 산화반응을 위해 박막형 전극을 제조하였다. 전극은 텅스텐 탄화물(WC)과 텅스텐 산화물($WO_3$), 그리고 백금(Pt) 타겟을 이용하였으며 그 구조적 특성과 전기화학적 특성을 TEM(Transmission electron microscopy와 CV(Cyclic Voltametry)를 통하여 촉매적 활성을 측정해 보았다. 같은 양의 백금과의 활성을 비교하고 활성을 확인하였다.

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Electrochemical Properties of Cu Current Collector with Li0.5La0.5TiO3 or Si Thin Film as a Li Free Anode (Li0.5La0.5TiO3와 Si박막을 갖는 구리 집전체의 Li free 음극으로써의 전기화학적 특성)

  • Lee Jae-Jun;Kim Soo-Ho;Lee Jong-Min;Yoon Young-Soo
    • Journal of the Korean Electrochemical Society
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    • v.9 no.1
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    • pp.34-39
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    • 2006
  • Electrochemical properties of Cu foil current collector with a $Li_{0.5}La_{0.5}TiO_3$ Cu a Si thin film deposited by r.f sputtering as an anode for Li free battery were evaluated. The Cu foil current collectors were lied in and out of plasma during sputtering process. The X-ray diffraction results indicated that the as-deposited Si and $Li_{0.5}La_{0.5}TiO_3$ thin films in and out of plasma did not show any crystalline difference. The $Li_{0.5}La_{0.5}TiO_3$ film in plasma and Si film out of plasma showed better cyclability since crystalline $Li_{0.5}La_{0.5}TiO_3$ has much higher ionic conductivity and crystalline Si film is much sensitive far volume change during charge-discharge process. These results suggested that the deposition of amorphous Si on Cu foil current collector is much better for fabrication of Li free battery and it can be useful for the unique battery with a cycling number constraint of below 10.

Electrodeposition of Cu(InxGa(1-x))Se2 Thin Film (CIGS 박막의 전착에 관한 연구)

  • Lee, Sang-Min;Kim, Young-Ho;Oh, Mi-Kyung;Hong, Suk-In;Ko, Hang-Ju;Lee, Chi-Woo
    • Journal of the Korean Electrochemical Society
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    • v.13 no.2
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    • pp.89-95
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    • 2010
  • The chalcopyrite $Cu(In_xGa_{(1-x)})Se_2$ (CIGS) is considered to be one of the effective light-absorbing materials for thin film photovoltaic solar cells. We describe the electrodeposition of CIGS thin films in ambient laboratory conditions, and suggest the electrochemical conditions to prepare stoichiometric CIGS thin films of Ga/(In + Ga) = 0.3. In acidic solutions containing $Cu^{2+}$, $In^{3+}$, $Ga^{3+}$ and $Se^{4+}$ ions, the CIGS films of different Cu/In/Ga/Se chemical compositions were electrodeposited onto Mo/Glass substrate. The structure, morphology and chemical composition of electrodeposited CIGS films were characterized by X-ray diffraction (XRD), Scanning electron microscopy (SEM), and Energy dispersive X-ray spectroscopy (EDS), respectively.

Analysis of Football Fans' Uniform Consumption: Before and After Son Heung-Min's Transfer to Tottenham Hotspur FC (국내 프로축구 팬들의 유니폼 소비 분석: 손흥민의 토트넘 홋스퍼 FC 이적 전후 비교)

  • Choi, Yeong-Hyeon;Lee, Kyu-Hye
    • Journal of Intelligence and Information Systems
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    • v.26 no.3
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    • pp.91-108
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    • 2020
  • Korea's famous soccer players are steadily performing well in international leagues, which led to higher interests of Korean fans in the international leagues. Reflecting the growing social phenomenon of rising interests on international leagues by Korean fans, the study examined the overall consumer perception in the consumption of uniform by domestic soccer fans and compared the changes in perception following the transfers of the players. Among others, the paper examined the consumer perception and purchase factors of soccer fans shown in social media, focusing on periods before and after the recruitment of Heung-Min Son to English Premier League's Tottenham Football Club. To this end, the EPL uniform is the collection keyword the paper utilized and collected consumer postings from domestic website and social media via Python 3.7, and analyzed them using Ucinet 6, NodeXL 1.0.1, and SPSS 25.0 programs. The results of this study can be summarized as follows. First, the uniform of the club that consistently topped the league, has been gaining attention as a popular uniform, and the players' performance, and the players' position have been identified as key factors in the purchase and search of professional football uniforms. In the case of the club, the actual ranking and whether the league won are shown to be important factors in the purchase and search of professional soccer uniforms. The club's emblem and the sponsor logo that will be attached to the uniform are also factors of interest to consumers. In addition, in the decision making process of purchase of a uniform by professional soccer fan, uniform's form, marking, authenticity, and sponsors are found to be more important than price, design, size, and logo. The official online store has emerged as a major purchasing channel, followed by gifts for friends or requests from acquaintances when someone travels to the United Kingdom. Second, a classification of key control categories through the convergence of iteration correlation analysis and Clauset-Newman-Moore clustering algorithm shows differences in the classification of individual groups, but groups that include the EPL's club and player keywords are identified as the key topics in relation to professional football uniforms. Third, between 2002 and 2006, the central theme for professional football uniforms was World Cup and English Premier League, but from 2012 to 2015, the focus has shifted to more interest of domestic and international players in the English Premier League. The subject has changed to the uniform itself from this time on. In this context, the paper can confirm that the major issues regarding the uniforms of professional soccer players have changed since Ji-Sung Park's transfer to Manchester United, and Sung-Yong Ki, Chung-Yong Lee, and Heung-Min Son's good performances in these leagues. The paper also identified that the uniforms of the clubs to which the players have transferred to are of interest. Fourth, both male and female consumers are showing increasing interest in Son's league, the English Premier League, which Tottenham FC belongs to. In particular, the increasing interest in Son has shown a tendency to increase interest in football uniforms for female consumers. This study presents a variety of researches on sports consumption and has value as a consumer study by identifying unique consumption patterns. It is meaningful in that the accuracy of the interpretation has been enhanced by using a cluster analysis via convergence of iteration correlation analysis and Clauset-Newman-Moore clustering algorithm to identify the main topics. Based on the results of this study, the clubs will be able to maximize its profits and maintain good relationships with fans by identifying key drivers of consumer awareness and purchasing for professional soccer fans and establishing an effective marketing strategy.