• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1998.06a
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• pp.149.1-150
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• 1998

• Journal of Life Science
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• v.28 no.10
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• pp.1132-1139
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• 2018

The stability of diverse cellular proteins in eukaryotes is regulated via ubiquitination. Moreover, E3 ligase plays a crucial role in determining substrate specificity and transfers ubiquitins into the substrates during the ubiquitination process. As a type of multi-subunit E3 ligase, cullin4 (CUL4)-based E3 ligase (CRL4) complex is involved in a variety of cellular processes, such as hormonal and stress responses in plants. In spite of several reports on the versatile roles of CRL4 in various signalings in Arabidopsis, CRL4's function in rice has been poorly known. To learn about CRL4-mediated cellular processes in rice in more detail, OsCUL4 that exhibits the highest homology with Arabidopsis CUL4 was isolated, and its expression patterns in various tissues and in response to plant hormones and abiotic stresses were monitored. Exogenous application of ABA or cytokinin increased the transcript levels of the OsCUL4 gene. Moreover, OsCUL4 was significantly upregulated in response to drought and salt stresses. These findings imply that OsCUL4 may be functionally related to ABA- and/or cytokinin-mediated cellular responses. OsCUL4 directly interacted with OsDDB1, an adaptor protein of CRL4, indicating that OsCUL4 can act as a scaffold protein of CRL4. An expression study on the OsCUL4 gene from this report could be used as a starting point to elucidate cellular responses in which a CRL4-mediated ubiquitination process is involved in rice.

• Korean Journal of Psychosomatic Medicine
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• v.10 no.1
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• pp.18-26
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• 2002

Objectives : The object of this study was to investigate the relationship between coping strategies and perceived stress or pain discomfort in patients with chronic low back pain. 80 patients with chronic low back pain and 100 normal controls participated in this study. Methods: Global assessment of recent stress (GARS) scale and Stress Response Inventory (SRI) were used to measure perception for stressors and stress responses. Coping scale and pain discomfort scale were used to measure coping strategies and pain perception. Results : Scores of perceived stress related to work or job, interpersonal relationship, changes in relationship, sickness or illness and the total scores on the GARS scale were significantly higher in those with chronic low back pain than normal controls. Scores of the SRI fatigue subscale scored significantly higher in those with chronic low back pain than normal controls. No significant difference was found on total scores of the pain discomfort scale between those with chronic low back pain and normal controls. The patients with chronic low back pain scored significantly higher on planful problem solving and positive reappraisal than normal controls. In the patient group, pain perception had significant positive correlations with total scores of the SRI and scores of stress perception related to illness or injury. The extent of escape-avoidance showed significant negative correlations with age, whereas the extent of distancing or escape-avoidance had significant negative correlations with the level of education. Significant difference was also found in accepting responsibilities between male subjects and females. However, no significant correlations were found between coping strategies and perceived stressors, stress responses or pain perception. Conclusion : The results suggest that patients with chronic low back pain were more likely to use more active coping strategies than normal controls, though the former had more perception for stressors than the latter. It was also found that coping strategies used by the patients were associated with sociodemographic factors, but that they were not associated with perceived stressors, stress responses or pain perception.

• The Journal of Korean Academic Society of Nursing Education
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• v.7 no.1
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• pp.157-169
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• 2001

The purposes of this study were to investigate stress responses of senior high school students(examines) preparing for college entrance examination and to identify the relationship between stress responses and self-esteem. The subjects of this study were 261 senior high school students from three high schools located in three different Gu Seoul Korea. Data were collected from 19th to 27th march, 1998. Stress responses were measured by SOS (symptoms of stress) inventory and self- esteem was measured by Rosenberg's self-esteem scale. The data were analyzed with descriptive statistics, t-test, ANOVA and Pearson's correlation coefficient. The results were as follows : 1. The mean SOS score of subjects was 1.22 and that showed statistically significant according to gender difference (t=-6.00, P<0.0001) and father's occupations (F=3.10, P=0.006). 2. The mean self-esteem score was 2.77 and that showed statistically significant difference according to economic status(F=3.96, P=0.02) and father's occupations (F=2.71, P=0.01). 3. There was significant negative correlation between the mean SOS score and the mean self-esteem score(r=-0.31, P=0.0001). In conclusion, the examines had very high physiological and psychological stress responses and especially female showed higher stress responses than male. For this reason, school nurses are recommended (1)to develop appropriate stress management technique, (2) to provide more intensive care for health of female examines Nursing intervention should be developed for enhancing self-esteem of examines because self-esteem is negatively correlated with SOS.

• Korean Journal of Environmental Biology
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• v.26 no.4
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• pp.271-278
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• 2008

The differences of several kinds of physiological responses between Commelina communis (C$_3$ plant) and Sedum sarmentosum (CAM plant: Crassulacean Acid metabolism) when both plants were exposed to water stress for 3 weeks were investigated. In case of Commelina it was shown a clear loss of water to 12% in three weeks, but no changes were observed in Sedum. Total chlorophyll content was also reduced to 57% in Commelina but not clear changes of chlorophyll content in Sedum. were observed for three weeks. In chlorophyll fluorescence experiments Fv/Fm ratios were reduced to 19% in Commelina, but no changes were observed in Sedum. There were very sensitive responses according to the different KCl concentrations and the stomatal aperture of epidermal strips was 12.8 ${\mu}m$ at 200 mM KCl in Commelina, but less than 3 ${\mu}m$ was observed at the same KCl concentration in Sedum. In addition, there were no chloroplasts in guard cells of Sedum, but most plants had chloroplasts including Commelina. From the above results, the ability of water stress resistance in Sedum. could be come from slow physiological metabolism including growth and less loss of water through unique stomatal characteristics.

• Korean Journal of Psychosomatic Medicine
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• v.4 no.2
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• pp.207-214
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• 1996

The study was designed to find out the effect of exam stress and vitamin B complex on hormones such as plasma ACTH, cortisol and prolactin. 21 medical students completed the whole period of the study. Global assessment of recent stress(GARS) scale and SCL-90-R were used to measure stress perception and psychopathology. Radioimmunoassay was used to assess plasma ACTH, cortisol and prolactin. Plasma ACTH level was significantly higher 2 weeks prior to examination and exam period, respectively, than 4 weeks prior to the exam. However, there were no significant differences in plasma cortisol and prolactin level among the three periods. No significant differences were also found in plasma ACTH, cortisol and prolactin level between vitamin and non-vitamin groups during each period. Scores of stress perception in economic area significantly had a positive correlation with plasma ACTH and prolactin level, respectively, 2 weeks prior to the exam. In psychopathology, scores of hostility subscale significantly had a Positive correlation with plasma ACTH level. There were no significant differences in change of each of the hormones over time as well as between vitamin and non-vitamin groups. In conclusion, it was found that ACTH was more sensitive to exam stress than cortisol or prolactin, and that vitamin B complex had no significant influence on ACTH, cortisol and prolactin level.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.22-29
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• 2011

Although reactive oxygen species (ROS) are inevitable by-products of many redox reactions in eukaryotic cells, they play a crucial role as signaling molecules in many cellular processes for development and defense response to abiotic stresses. The biphasic ROS production which was peaked twice in a first transient phase and a second massive phase was occurred after treatment of abiotic stress such as oxidative stress, high salinity. This biphasic generation of ROS was followed by the biphasic production of stress hormone, ethylene. The mechanism of interactions between ROS and ethylene biosynthesis is studied in tobacco (Nicotiana tabaccum L.) plants under the abiotic stresses. The stress-induced ethylene production was significantly inhibited in RbohD-AS and RbohF-AS, in which antisense expression of NADPH oxidase genes was performed. The accumulation of ROS, which was determined by DAB and DCFH-DA staining, was significantly decreased after abiotic stresses in transgenic plants. The suppression of signaling with ethylene and ROS induced more tolerance in response to abiotic stress. The transgenic plants were more tolerant in MS medium supplemented with salinity stress in contrast with wild-type. Stress-induced cell damage determined by DNA fragmentation was decreased at phase II in those transgenic plants. Therefore, the first burst of ROS is more responsible for making a role as a signaling molecule during stress-induced response. These results suggested that ethylene and ROS act in a positive feedback cycle that results in mutual enhancement of ethylene and ROS production during stress-induced cell death.

• Sleep Medicine and Psychophysiology
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• v.7 no.2
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• pp.109-114
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• 2000

Objectives: This study was performed to examine the characteristic cardiovascular response patterns associated with the imagination and discussion of anger-provoked events in patients with hwa-byung. Methods: Forty-three female patients with hwa-byung were evaluated with the Korean version of the State-Trait Anger Expression Inventory, the State-Trait Anxiety Inventory, Hamilton Rating Scale for Anxiety, Beck Depression Inventory, and Hamilton Rating Scale for Depression just before the task. Subjective Units of Distress (SUDS) and Vividness of the event (VIVID) during the imagination and discussion of the event were evaluated immediately after tasks. Blood pressure (BP) and heart rate (HR) during baseline, rest, and tasks were also evaluated. Results: Both startle and recovery responses of BP, startle response of HR, SUDS, and VIVID in discussion task were significantly larger than in the imagination task. Conclusion: Results suggest that it is undesirable for the patients to excessively and repeatedly recall and talk about the anger events.

• Journal of Life Science
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• v.14 no.4
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• pp.657-663
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• 2004

Tobacco transformants harboring cinnamic acid 4-hydroxylase gene (C4H) fused with susquiterpene cyclase promoter was developed in order to regulate biosynthesis of phenolic compounds by the expression of the introduced gene. Twenty transformants for each specific promoter were used to analyze the incorporation of the chimeric genes by PCR and Southern blot analysis. PCR products of NPTII(neomycin phosphotransferase) gene (553bp) were detected in the transgenic tobacco plants. The incorporation of the chimeric gene was confirmed in the Southern blot analysis. C4H activity in the transgenic plants was elevated by UV-irradiation and its level was higher compared to that of control plants.

• Korean Journal of Poultry Science
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• v.44 no.1
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• pp.1-9
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• 2017

The objective of the present study was to determine the expression of genes associated with lipopolysaccharide (LPS)-induced stressor in two breeds of chickens: the Korean native chicken (KNC) and the White Leghorn chicken (WLH). Forty chickens per breed, aged 40 weeks, were randomly allotted to the control (CON, administered the saline vehicle) and LPS-injected stress groups. Samples were collected at 0 and 48 h post-LPS injection, and total RNA was extracted from the chicken livers for RNA microarray and quantitative real-time polymerase chain reaction (qRT-PCR) analyses. In response to LPS, 1,044 and 1,193 genes were upregulated, and 1,000 and 1,072 genes were downregulated in the KNC and WLH, respectively, using a ${\geq}2$-fold cutoff change. A functional network analysis revealed that stress-related genes were downregulated in both KNC and WLH after LPS infection. The results obtained from the qRT-PCR analysis of mRNA expression of heat shock 90 (HSP90), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), activating transcription factor 4 (ATF4), sterol regulatory element-binding protein 1 (SREBP1), and X-box binding protein 1 (XBP1) were confirmed by the results of the microarray analysis. There was a significant difference in the expression of stress-associated genes between the control and LPS-injected KNC and WLH groups. The qRT-PCR analysis revealed that the stress-related $HSP90{\alpha}$ and HMGCR genes were downregulated in both LPS-injected KNC and WLH groups. However, the HSP70 and $HSP90{\beta}$ genes were upregulated only in the LPS-injected KNC group. The results suggest that the mRNA expression of stress-related genes is differentially affected by LPS stimulation, and some of the responses varied with the chicken breed. A better understanding of the LPS-induced infective stressors in chicken using the qRT-PCR and RNA microarray analyses may contribute to improving animal welfare and husbandry practices.