• Journal of Food Hygiene and Safety
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• v.34 no.3
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• pp.227-234
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• 2019

An analytical method was developed for the determination of oxytetracycline in agricultural products using the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method by liquid chromatography-tandem mass spectrometry (LC-MS/MS). After the samples were extracted with methanol, the extracts were adjusted to pH 4 by formic acid and sodium chloride was added to remove water. Dispersive solid phase extraction (d-SPE) cleanup was carried out using $MgSO_4$ (anhydrous magnesium sulfate), PSA (primary secondary amine), $C_{18}$ (octadecyl) and GCB (graphitized carbon black). The analytes were quantified and confirmed with LC-MS/MS using ESI (electrospray ionization) in positive ion MRM (multiple reaction monitoring) mode. The matrix-matched calibration curves were constructed using six levels ($0.001{\sim}0.25{\mu}g/mL$) and coefficient of determination ($r^2$) was above 0.99. Recovery results at three concentrations (LOQ, $10{\times}LOQ$, and $50{\times}LOQ$, n=5) were from 80.0 to 108.2% with relative standard deviations (RSDs) less than of 11.4%. For inter-laboratory validation, the average recovery was in the range of 83.5~103.2% and the coefficient of variation (CV) was below 14.1%. All results satisfied the criteria ranges requested in the Codex guidelines (CAC/GL 40-1993, 2003) and the Food Safety Evaluation Department guidelines (2016). The proposed analytical method was accurate, effective and sensitive for oxytetracycline determination in agricultural commodities. This study could be useful for safety management of oxytetracycline residues in agricultural products.

• Journal of KIISE:Information Networking
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• v.35 no.5
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• pp.453-463
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• 2008

The missing child problem is no more a personal problem. It became a social problem that all parents must consider. To this, this study applies USN/RFID technology integrated with GIS for the prevention of missing child. Although RFID is not designed for location sensing, but now it is regarded as a device to facilitate real time location awareness. Such advantages of RFID can be integrated with 4S(GIS/GPS/LBS/GNSS) achieving much synergy effects. In order to prevent kidnapping and missing child, it is necessary to provide a missing child preventing system using a ubiquitous computing system. Therefore, the missing child preventing system has been developed using high-tech such as RFID, GPS network, CCTV, and mobile communication. The effectiveness of the missing child prevention system can be improved through an accurate location tracking technology. This study propose and test a location sensing system using the active RFID tags. This study verifies technical applied service, and presents a system configuration model. Finally, this paper confirms missing child prevention system utilization possibility.

• International Journal of Highway Engineering
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• v.10 no.1
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• pp.123-134
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• 2008

Since the end treatments of guardrails installed on domestic roads have the shapes which can easily penetrate and turnover a vehicle, the occupant can be subjected to severe injury when a vehicle impacts the end treatments. In this study, the criteria of performance evaluation for end treatments are suggested which are suitable to domestic road circumstances. Based on the investigation for the installation and studies instances of end treatments, the mechanism of end treatments is examined and the new end treatment suitable to domestic road circumstances is suggested. The suggested end treatment was verified by computer simulation using d LS-DYNA programs and satisfied the suggested performance evaluation criteria for end treatments. And the developed end treatment was verified by full-scale vehicle crash test and satisfied the following three primary appraisal factors of the suggested performance evaluation criteria for end treatments; occupant risk criteria, structural adequacy, and after-collision vehicle trajectory. This study is the first to develop end treatments considering the occupant safety in Korea. Therefore, If the developed end treatments is installed on roads and highways, it can be expected that it will reduce the grave situation of end treatment accidents and increase the safety of roads.

• Journal of the Korean association of regional geographers
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• v.11 no.4
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• pp.523-535
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• 2005

This study is aimed at researching the applicability of teaching-learning models in highschool geography class by designing the models on the basis of geographical experience the learners go through everyday life. The procedures and results of the application of the models are as followed. First, the systematization of the teaching concepts should be preceded to internalize the learners cognitive development, that is, to systemize cognitive structure. The concrete learning points of geographical concepts from the units about Migration and Population Changes are systemized with 'migration' as a higher concept, 'moving type' as basic concept, 'moving factors' as the lower concept. Everyday geographical experiences the students can go through are surveyed. Second, as preparation for the geography class, hand-outs about family-moving history and the change of the family number were used as basic material for real class teaching activity, showing the learners' general concepts are very effective as basic units which can be easily understood and accessed to. Third, with the experimental class, the geography class should secure the flexibility on the teaching-learning process. The result of applying the newly developed teaching-learning model to actual geography classes was that experimental group had higher achievement rate than the compared group with general teaching-learning model applied to. The result of analyzing students' response of the new teaching-learning model was that the students were interested and satisfied emphatically and they showed positive response in regard to practical use of the contents. Here, it is noticeable that the new teaching-learning model causes the students to be interested. But it's also found that there's no big difference in improving the students' inquisitive mind.

• Parasites, Hosts and Diseases
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• v.34 no.3
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• pp.191-196
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• 1996

Polymerase chain reaction (PCR) is a powerful technique to detect scanty amount of DNA from living organisms. The present study intended to develope specific primers for PCR diagnosis of pneumocystosis and to evaluate diagnostic efficacy by preparation of template DNAs from invasive BAk fluid and also to screen serum or blood as a non-invasive specimen. Albino rats of Wistar or Fischer strains were experimentally infected by Pneumocwstis ccrinii. Extracted DNAs or cell Iysates of their blood, bronchoalveolar lavage fluid, and lung homogenate were used as the tenlplate DNA. Primers were synthetic oligonucleotides among 16s rDNA sequences. All of the primer combinations gave PCR products, but the primer pair of #24 and #27 gave best quality product of 666 bp. The sensitivity of PCR with Iysates of BAk fluid was 57.7% but it increased to 84.6% with extracted DNAs. None of BAL Iysate or DNA was positive among 13 microscopically negatives. The serum DNAs were positive only in 2 cases out of 20 morphologically positive rats. DNAs of human, rat, other parasites, yeast, and microorganisms were negative. The findings suggest that the present primers are specific but simple Iysate of BAL fluid is not sensitive. PCR may be used as a routine diagnostic method of pneumocystosis if simple and rapid preparation of non-invasive clinical specimens are available.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.3
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• pp.304-314
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• 2010

This study was carried out to find out potential use of ochre as an agent to reduce phosphorus content in water. Ochre is a by-product from treatment of acid mine drainage (AMD) which is composed mostly of $Fe_2O_3$, $Fe_2O_3{\cdot}H_2O$, $FeO{\cdot}OH$ and $Fe(OH)_3$. Three ochre samples (ochre-H, ochre-D and ochre-S) were collected from three treatment facilities in Gangwon province. Physico-chemical characteristics of three ochre samples including pH, electrical conductivity, total phosphorus, available phosphorus, particle size distribution were analyzed. Scanning electron microscopy (SEM) energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and X-ray fluorescence (XRF) analysis were also carried out. In addition, experiments for phosphorus removal from water was performed. Calcium content of ochre-H was higher than that of ochre-D and ochre-S, whereas iron content of ochre-H was lower than that of ochre-D and ochre-S. All the phosphorus in water up to maximum 191,411 mg $kg^{-1}$ per unit mass of ochre was removed with ochre-H. Ochre has immense potential as an agent to reduce phosphorus content in water.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.6
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• pp.549-558
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• 2012

A topology optimization method for phononic crystals is developed for the design of sound barriers, using the level set approach. Given a frequency and an incident wave to the phononic crystals, an optimal shape of periodic inclusions is found by minimizing the norm of transmittance. In a sound field including scattering bodies, an acoustic wave can be refracted on the obstacle boundaries, which enables to control acoustic performance by taking the shape of inclusions as the design variables. In this research, we consider a layered structure which is composed of inclusions arranged periodically in horizontal direction while finite inclusions are distributed in vertical direction. Due to the periodicity of inclusions, a unit cell can be considered to analyze the wave propagation together with proper boundary conditions which are imposed on the left and right edges of the unit cell using the Bloch theorem. The boundary conditions for the lower and the upper boundaries of unit cell are described by impedance matrices, which represent the transmission of waves between the layered structure and the semi-infinite external media. A level set method is employed to describe the topology and the shape of inclusions. In the level set method, the initial domain is kept fixed and its boundary is represented by an implicit moving boundary embedded in the level set function, which facilitates to handle complicated topological shape changes. Through several numerical examples, the applicability of the proposed method is demonstrated.

• Journal of Korea Water Resources Association
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• v.47 no.7
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• pp.599-614
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• 2014

In this study, two dimensional finite volume model was parallelized to improve computing time, which has been developed to be able to apply for the mixed meshes of triangle and quadrilateral. MPI scheme which is free from limitation of the number of cores was applied, and non-blocking point-to-point communication was used for fluxes and time steps calculation domain. The developed model is applied to analyze dam break in a L-shaped experimental channel with $90^{\circ}$ bend and Malpasset dam breach event to calibrate the consistency between parallelized model and existing model and examine the speed-up and efficiency of computing time. Computational speed-up about the size of the input data was considered by simulating 4 cases classified by the number of meshes, Consequently, the simulation results reached a satisfactory accuracy compared to measured data and the results from existing model, and achieved more than 3 times benefit of computational speed-up against computing time of existing model. Simulation results of 3 cases classified by the size of input data lead us to the conclusion that it is important to use proper size of input data and the number of process in order to minimize the communication overhead.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.1
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• pp.27-35
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• 2015

The material properties of crack growth models at an elevated temperature were derived from the results of numerous crack growth tests for Mod.9Cr-1Mo (ASME Grade 91) steel specimens under fatigue loading and creep loading at an elevated temperature. These crack growth models were needed for defect assessment under creep-fatigue loading. The mathematical crack growth rate models for fatigue crack growth (FCG) and creep crack growth (CCG) were determined based on the test results, and the models were compared with those of the French design code RCC-MRx to investigate the conservatism of the code. The French design code RCC-MRx provides an FCG model and a CCG model for Grade 91 steel in Section III Tome 6. It was shown that the FCG model of RCC-MRx is conservative, while the CCG model is non-conservative compared with the present test data. Thus, it was shown that further validation of the property was required. Mechanical strength tests and creep tests were also conducted, and the test results were compared with those of RCC-MRx.

• Science of Emotion and Sensibility
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• v.18 no.1
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• pp.39-48
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• 2015

It is known that P300-based concealed information test (P300 CIT) was not greatly affected by the traditional countermeasures. This study was to test whether P300 CIT is affected by the new countermeasures. We used three types of countermeasures. First type was a sequential countermeasure in which participants had to respond in alternating ways to irrelevants by pressing the left index finger covertly when the encountered irrelevant firstly, by wiggling the right big toe inside the shoe when encountered irrelevant secondly, by imaging his or her mother's name when encountered irrelevant thirdly, and by imaging his or her father's name when encountered irrelevant fourthly until all stimuli were presented. Second type was a partial matching and physical countermeasure. Participants in this type were asked to press the left index finger imperceptibly after one of the irrelevants and wiggle the right big toe after another of the irrelevants. Third type was a partial matching and mental countermeasure. Participants were required to imagine mother's name for one irrelevant and father's name for another irrelevant. The results showed that contrary to our expectation, the use of sequential countermeasure increased the detection rate from 77% to 92%. The partial matching countermeasure had a negative effect on P300 CIT. The physical countermeasure decreased the detection rate from 77% to 46%, and the mental countermeasure decreased the detection rate from 100% to 69%. The necessity for the development of methods to prevent or detect countermeasure is discussed.