• Title/Summary/Keyword: 수준교차

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Analysis of Foodborne Pathogens in Food and Environmental Samples from Foodservice Establishments at Schools in Gyeonggi Province (경기지역 학교 단체급식소 식품 및 환경 중 식중독균 분석)

  • Oh, Tae Young;Baek, Seung-Youb;Koo, Minseon;Lee, Jong-Kyung;Kim, Seung Min;Park, Kyung-Min;Hwang, Daekeun;Kim, Hyun Jung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.12
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    • pp.1895-1904
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    • 2015
  • Foodborne illness associated with food service establishments is an important food safety issue in Korea. In this study, foodborne pathogens (Bacillus cereus, Clostridium perfringens, Escherichia coli, pathogenic Escherichia coli, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, and Vibrio parahaemolyticus) and hygiene indicator organisms [total viable cell counts (TVC), coliforms] were analyzed for food and environmental samples from foodservice establishments at schools in Gyeonggi province. Virulence factors and antimicrobial resistance of detected foodborne pathogens were also characterized. A total of 179 samples, including food (n=66), utensil (n=68), and environmental samples (n=45), were collected from eight food service establishments at schools in Gyeonggi province. Average contamination levels of TVC for foods (including raw materials) and environmental samples were 4.7 and 4.0 log CFU/g, respectively. Average contamination levels of coliforms were 2.7 and 4.0 log CFU/g for foods and environmental swab samples, respectively. B. cereus contamination was detected in food samples with an average of 2.1 log CFU/g. E. coli was detected only in raw materials, and S. aureus was positive in raw materials as well as environmental swab samples. Other foodborne pathogens were not detected in all samples. The entire B. cereus isolates possessed at least one of the diarrheal toxin genes (hblACD, nheABC, entFM, and cytK enterotoxin gene). However, ces gene encoding emetic toxin was not detected in B. cereus isolates. S. aureus isolates (n=16) contained at least one or more of the tested enterotoxin genes, except for tst gene. For E. coli and S. aureus, 92.7% and 37.5% of the isolates were susceptible against 16 and 19 antimicrobials, respectively. The analyzed microbial hazards could provide useful information for quantitative microbial risk assessment and food safety management system to control foodborne illness outbreaks in food service establishments.

A Study on Interactions of Competitive Promotions Between the New and Used Cars (신차와 중고차간 프로모션의 상호작용에 대한 연구)

  • Chang, Kwangpil
    • Asia Marketing Journal
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    • v.14 no.1
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    • pp.83-98
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    • 2012
  • In a market where new and used cars are competing with each other, we would run the risk of obtaining biased estimates of cross elasticity between them if we focus on only new cars or on only used cars. Unfortunately, most of previous studies on the automobile industry have focused on only new car models without taking into account the effect of used cars' pricing policy on new cars' market shares and vice versa, resulting in inadequate prediction of reactive pricing in response to competitors' rebate or price discount. However, there are some exceptions. Purohit (1992) and Sullivan (1990) looked into both new and used car markets at the same time to examine the effect of new car model launching on the used car prices. But their studies have some limitations in that they employed the average used car prices reported in NADA Used Car Guide instead of actual transaction prices. Some of the conflicting results may be due to this problem in the data. Park (1998) recognized this problem and used the actual prices in his study. His work is notable in that he investigated the qualitative effect of new car model launching on the pricing policy of the used car in terms of reinforcement of brand equity. The current work also used the actual price like Park (1998) but the quantitative aspect of competitive price promotion between new and used cars of the same model was explored. In this study, I develop a model that assumes that the cross elasticity between new and used cars of the same model is higher than those amongst new cars and used cars of the different model. Specifically, I apply the nested logit model that assumes the car model choice at the first stage and the choice between new and used cars at the second stage. This proposed model is compared to the IIA (Independence of Irrelevant Alternatives) model that assumes that there is no decision hierarchy but that new and used cars of the different model are all substitutable at the first stage. The data for this study are drawn from Power Information Network (PIN), an affiliate of J.D. Power and Associates. PIN collects sales transaction data from a sample of dealerships in the major metropolitan areas in the U.S. These are retail transactions, i.e., sales or leases to final consumers, excluding fleet sales and including both new car and used car sales. Each observation in the PIN database contains the transaction date, the manufacturer, model year, make, model, trim and other car information, the transaction price, consumer rebates, the interest rate, term, amount financed (when the vehicle is financed or leased), etc. I used data for the compact cars sold during the period January 2009- June 2009. The new and used cars of the top nine selling models are included in the study: Mazda 3, Honda Civic, Chevrolet Cobalt, Toyota Corolla, Hyundai Elantra, Ford Focus, Volkswagen Jetta, Nissan Sentra, and Kia Spectra. These models in the study accounted for 87% of category unit sales. Empirical application of the nested logit model showed that the proposed model outperformed the IIA (Independence of Irrelevant Alternatives) model in both calibration and holdout samples. The other comparison model that assumes choice between new and used cars at the first stage and car model choice at the second stage turned out to be mis-specfied since the dissimilarity parameter (i.e., inclusive or categroy value parameter) was estimated to be greater than 1. Post hoc analysis based on estimated parameters was conducted employing the modified Lanczo's iterative method. This method is intuitively appealing. For example, suppose a new car offers a certain amount of rebate and gains market share at first. In response to this rebate, a used car of the same model keeps decreasing price until it regains the lost market share to maintain the status quo. The new car settle down to a lowered market share due to the used car's reaction. The method enables us to find the amount of price discount to main the status quo and equilibrium market shares of the new and used cars. In the first simulation, I used Jetta as a focal brand to see how its new and used cars set prices, rebates or APR interactively assuming that reactive cars respond to price promotion to maintain the status quo. The simulation results showed that the IIA model underestimates cross elasticities, resulting in suggesting less aggressive used car price discount in response to new cars' rebate than the proposed nested logit model. In the second simulation, I used Elantra to reconfirm the result for Jetta and came to the same conclusion. In the third simulation, I had Corolla offer $1,000 rebate to see what could be the best response for Elantra's new and used cars. Interestingly, Elantra's used car could maintain the status quo by offering lower price discount ($160) than the new car ($205). In the future research, we might want to explore the plausibility of the alternative nested logit model. For example, the NUB model that assumes choice between new and used cars at the first stage and brand choice at the second stage could be a possibility even though it was rejected in the current study because of mis-specification (A dissimilarity parameter turned out to be higher than 1). The NUB model may have been rejected due to true mis-specification or data structure transmitted from a typical car dealership. In a typical car dealership, both new and used cars of the same model are displayed. Because of this fact, the BNU model that assumes brand choice at the first stage and choice between new and used cars at the second stage may have been favored in the current study since customers first choose a dealership (brand) then choose between new and used cars given this market environment. However, suppose there are dealerships that carry both new and used cars of various models, then the NUB model might fit the data as well as the BNU model. Which model is a better description of the data is an empirical question. In addition, it would be interesting to test a probabilistic mixture model of the BNU and NUB on a new data set.

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Association between Oral Health Related Quality of Life(OHQoL) and Oral Health Outcome in Adults over 50 Years Old (50대 이상 장노년층의 구강건강 관련 삶의 질과 구강 건강 결과의 관련성)

  • Cho, Young-Sik;Lim, Soon-Ryun;Hwang, Hye-Rim
    • Journal of dental hygiene science
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    • v.11 no.4
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    • pp.305-310
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    • 2011
  • The purpose of this study was to investigate association between oral health related quality of life(OHRQoL) and health outcome, such as evaluated oral health state, perceived health state and perceived oral health state. 148 of individuals over 50 years old completed a self-reported questionnaire on Oral Health Impact Profile(OHIP-14) and oral examination was perfomed. 9.5% of respondents reported no problems or discomfort using OHIP-14. The most frequently reported problems were physical pain(69.8%) and psychological discomfort(63.5%). 43.9% of the individuals rated their health and oral health as poor and 54.7% were dissatisfied with their oral health. The study showed association between number of DMFT, missing teeth, perceived health state, perceived oral health state, oral health satisfaction and oral health related quality of life. Perceived heath sate was correlated with oral health related quality of life. But the study showed no association between DMFT, number of missing teeth and oral health related quality of life.

Factors Affecting Occupation Awareness for Dental Hygienist in High School Students (고등학생의 치과위생사에 대한 직업 인지 관련 요인)

  • Cho, Young-Sik;Hwang, Hye-Rim;Joo, Seung-Mi;Choi, Jung-Yoon;Hwang, Mi-Ra
    • Journal of dental hygiene science
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    • v.11 no.4
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    • pp.293-297
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    • 2011
  • The purpose of this study was to evaluate recognition of occupation title for dental hygienist in high school students. Total 210 students of one high school completed a self-reported questionnaire on sociodemographic characteristics, dental service utilization and occupation title awareness for health care personnels. Female and science tract students showed higher level of awareness for dental hygienist but showed no differences according to dental service utilization pattern. The study showed association between occupation recognition for dental hygienist and other health technicians. But there were no association between occupation awareness for dental hygienist and dentist, nurse. Gender, occupation recognition for medical laboratory technologists and physical therapist affect occupation recognition for dental hygienist.

Drinking conditions of adolescents ; Korea Youth Risk Behavior Web-based Survey (청소년의 음주 실태 ; 청소년 건강행태 온라인 조사를 중심으로)

  • Choi, Young-Sil
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.9
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    • pp.186-192
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    • 2017
  • Thepurpose of this study was to provide a basic data health plan & education program for adolescents by examining the recent 3 year Korea Youth Risk Behavior Web-based Survey data obtained in 2013, 2014 and 2015. The data was analyzed with theSPSS 18.0 statistical program using the t-test and cross-analysis. After confirming the drinking habits of teenagers in Korea, the issue was slightly lower in the issue of problem drinking. However, the first time for drinking alcohol was the 2nd grade of middle school(21.5%) and the average alcohol intake was 20 to 29 days(3.8%) and usually consisted ofmore than two bottles (12.2%)s. Also, the method of buying liquor was found to be mainly at convenience stores(32%), and the adolescents werefound not to have been trained for drinking(52%). As a result of comparing the general characteristics of these items, it was found that there was a significant difference between the coeducation type, the high school type, the trace in the residence type, and the 'middle-high level' in terms of the socio-economic level.When comparing the results of the drinking behavior during these three years, the outcome of the trend is still noteworthy, as health education for drinking alcohol is still important, and attention needs to be paid to this problem.

A Study on Factors Affecting Middle-aged Men's Suicidal Ideation (중년 남성의 자살생각 영향요인)

  • Lee, Seon-Young;Heo, Myoung-Lyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.7
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    • pp.4777-4785
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    • 2015
  • This study attempted to provide basic data for screening high-risk subjects by confirming suicidal thinking intensity and by identifying its influential factors in mid-aged men. Data No.3 from third National Health and Nutrition Survey in 2012 by the CDCP were used with 40-64 years middle-aged men targets. The collected data were analyzed via applying weight by using the complex sample analysis method of SPSS 19.0 program, frequency analysis, cross-analysis, and logistic regression analysis. The result revealed that 10% of middle-aged men showed suicidal thinking, and low-income earners had suicidal thinking 1.972 times higher than usual, the unmarried 2.587 times higher than men with spouses, men without spouses 1.482 times higher than men with spouses, the underweighted 18.183 times higher the obese. The blue-collared and the unemployed had suicidal thinking 1.349 and 13.342 times higher than the white-collared respectively. Furthermore, inactive subjects showed suicidal thinking 2.998 times higher than active subjects. Therefore, to prevent suicide in mid-aged men, separating these high-risk subjects in primary screening is needed for management, and suicide prevention programs should be developed at social and political level that include the information considering stress, depression, and obesity.

Awareness on the Establishing and Operation of the Makerspaces in School Libraries (학교도서관 메이커스페이스 조성 및 운영에 대한 인식)

  • Kang, Bong-Suk;Jung, Youngmi
    • Journal of the Korean Society for Library and Information Science
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    • v.52 no.3
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    • pp.171-192
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    • 2018
  • With the spread of the maker movement and matching of the standards for the 21st century learners and the maker competencies, there is a social and temporal need for makerspaces building and maker education in school libraries, Prior to the establishment of the school library makerspaces, we intended to investigate the awareness of the school librarian on the creation and operation of the school library makerspaces. For this purpose, a questionnaire was constructed based on the theoretical review and the response data of 171 school librarians were collected through a web survey. Technical statistics, cross-analysis, and ANOVA were conducted using SPSS window 19.0 and content analysis was conducted on open-ended questions. The Questionnaires consisted of questions about whether the school library makerspace was installed or not and necessity, reason, and difficulty in creating and operating the makerspace. As a result of the study, it was found that the establishment of the school library makerspace was very low at 2.3%, and the recognition of the makerspace of the school librarian was below the normal level. On the other hand, the perception of necessity appeared to be more than normal, and the school library was generally considered to be a suitable place for the makerspace installation. However, negative opinions about the school library makerspaces were also raised in various aspects.

Analysis of Accident Characteristics and Improvement Strategies of Flash Signal-operated Intersection in Seoul (서울시 점멸신호 운영에 따른 교통사고 분석 및 개선방안에 관한 연구)

  • Kim, Seung-Jun;Park, Byung-Jung;Lee, Jin-Hak;Kim, Ok-Sun
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.13 no.6
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    • pp.54-63
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    • 2014
  • Traffic accident frequency and severity level in Korea are known to be very serious. Especially the number of pedestrian fatalities was much worse and 1.6 time higher than the OECD average. According to the National Police Agency, the flash signals are reported to have many safety benefits as well as travel time reduction, which is opposed to the foreign studies. With this background of expanding the flash signal, this research aims to investigate the overall impact of the flash signal operation on safety, investigating and comparing the accident occurrence on the flash signal and the full signal intersections. For doing this accident prediction models for both flash and full signal intersections were estimated using independent variables (geometric features and traffic volume) and 3-year (2011-2013) accident data collected in Seoul. Considering the rare and random nature of accident occurrence and overdispersion (variance > mean) of the data, the negative binomial regression model was applied. As a result, installing wider crosswalk and increasing the number of pedestrian push buttons seemed to increase the safety of the flash signal intersections. In addition, the result showed that the average accident occurrence at the flash signal intersections was higher than at the full signal-operated intersections, 9% higher with everything else the same.

Permission Evaluation of the Price Hike of University Meal Service (대학교급식의 가격 상승 허용 평가)

  • Lee, Yun-Jung;Han, Kyung-Su
    • Culinary science and hospitality research
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    • v.15 no.4
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    • pp.115-130
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    • 2009
  • The price hike of university meal service hasn't increased as much as the rise in prices. Accordingly, it is necessary to make the school meal service better and make its image students think of improved, and it needs a proper increase. This study examines the permission evaluation of price hike of university meal service by analyzing students' behavior of using school meal service, restaurants they use, the price range of the meal service. It also investigates how the management of university meal service influences its price hike rate. The result of the study is as follows. Students are sensitive to price increase of school meal service because it hasn't been improved compared with its price. As a result of the evaluation of satisfaction with price in school meal service, its quality is bad compared with the restaurants outside school even though there isn't much difference between their prices. In addition, the cross tabulation analysis shows that there is significant difference between the restaurants students use and the price hike rate; there isn't significant difference between the price range of the meal service and the price hike rate; there is significant difference between the proper management of the meal service and the price hike rate. This study has a limit because it analyzes proper price and the quality of the restaurants comparing school cafeterias and the restaurants outside a university; however, it should be basic information for the improvement of university meal service in that the second most important reason why university students use a school cafeteria is price.

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Expression, Purification and Characterization of Yeast Thioredoxin System. (Yeast Thioredoxin System의 발현, 정제 및 특성조사)

  • 정진숙;김명희;김강화
    • Microbiology and Biotechnology Letters
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    • v.26 no.6
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    • pp.483-489
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    • 1998
  • We carried out the expression and characterization of yeast thioredoxin system including thioredexin 1 (Trx1), Trx2, thioredoxin reductase (TR), and a novel thioredoxin (Trx3), which was reported in the data base of Saccharomyces genome. The Trx1, 2 and TR were expressed as soluble proteins in E. coli and the sizes of purified proteins were equal to the reported their molecular weights. The expressed Trx3 was found in both soluble fraction and precipitate. The size of Trx3 purified from soluble fraction of E. coli crude extracts was estimated as 14 kDa on SDS-PAGE instead of 18 kDa for Trx3 in precipitate. N-terminal amino acid sequence of the small size of purified Trx3 from soluble fraction was analyzed as FQSSYTS which is correspond to the sequence from 20 to 26 for Trx3. Trx3 together with thioredoxin reductase and NADPH was able to reduce the disulfide bridge of insulin and 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB). Trx3 stimulated the antioxidant effect of thioredoxin peroxidase 1 (TPx1) which inhibited inactivation of glutamine synthetase (GS) in dithiothreitol (DTT) containing metal catalyzed oxidation system. The stimulation effect of Trx3 was 10% of the effect of either Trx1 or Trx2. In addition, Trx3 could reduce the disulfide of TPx to thiol, so that the TPx had thioredoxin dependant peroxidase activity. In western blotting analysis, antibodies against purified Trx3 did not cross-react with crude extracts of yeast, purified Trx1, and Trx2 proteins. But, in PCR reaction using the cDNA library of yeast as a template, gene encoding of trx3 was amplified.

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