Pinus pumila, which occurs in the northeast Asia, is found limitedly in Daecheongbong area of Mt. Seorak in the South Korea. This population was chosen to study spatial pattern, genetic diversity and spatial genetic structure. There were 48 polymorphic and 30 monomorphic I-SSR markers. A total of 65 individuals which distributed in the study site (40 m ${\times}$ 70 m) showed weakly aggregate distribution (Aggregate Index = 0.871). A total of 40 genets were observed from 65 individuals through I-SSR genotype comparison. Proportion of distinguishable genotype (G/N), genotype diversity (D) and genotype evenness (E) were 61.5%, 0.977 and 0.909, respectively. In spite of the small number and the limited distribution, Shannon's diversity index (I = 0.567) was relatively high as compared with those of other plant species. Spatial autocorrelation using Tanimoto's distance showed that the genetic patch was established within 12 m. Based on Mantel tests, there was relatively low correlation between genetic distance and geographic distance. Therefore, it seems the P. pumila population was formed by many parent trees in early stage. For ex situ genetic conservation of P. pumila, the sampling strategy is efficient at least above 12 m between individual trees.
There are various methods to evaluate tree vigor. Cambial electrical resistance represents tree vigor using the method of electrophysiological diagnosis. This study investigated the vigor of several tree species using Shigometer, and compared the differences among the species. The factors, such as foliation, trunk orientation and bark temperature, which affect electrical resistance were also investigated. The needle penetration into cambium was controlled to keep the depth consistent in order to minimize measurement error. Each of three trees were selected from Zelkova serrata, Ginkgo biloba, Metasequoia glyptostroboides, Pinus koraiensis, and Liriodendron tulipifera. The electrical resistances were measured at 60 and 120 cm height of the stem in 4 directions from March until May 2011. The soil conditions in surrounding areas and tree stress responses were also measured. The results were analyzed for the relationship between electrical resistance and the affecting factors. The electrical resistance showed a relatively higher level before foliation until mid-March. The values started to decline from April and recorded a minimal level on May 11. The changes of soil moisture, soil electric conductivity, and tree stress responses during the measurement period showed a similar trend to that of electrical resistance. The Pinus koraiensis, an evergreen conifer, showed few changes on the electrical resistance values during the measurement period. Zelkova serrata, Ginkgo biloba, and Metasequoia glyptostroboides showed the highest bark temperatures and lowest electrical resistances at their south-facing stem. Shigometer can provide measures simple to assess tree vigor in the fields, and to the management of trees.
Contents of immunoreactive ${\beta}-endorphin$ and maximum of $^3H-morphine$ binding was measured in the rat midbrain homogenates from different subgroups at 24 hour interval over 24 hours. Animals were adapted to the light-dark cycle(L : D, 12: 12) or constant darkness (D : D, 12 : 12) for 3 weeks. After the adaptation, 0.5ml of physiologic saline or drug was administered twice a day for 2 weeks. A highly significant circadian rhythm with the peak$(94.8{\pm}7.7\;fmole/mg\;protein)$ at 06:00 and the nadir $(27.6{\pm}2.4\;fmole/mg\;protein)$ at 18:00 was observed in constant of group. Constant dark or treatment of reserpine, pargyline, imipramine, amphetamine and chlorpromazine modified the diurnal rhythm in the time of peak and nadir, shape, phase amplitude and 24 hour mean of ${\beta}-endorphin$ contents. Opiate receptor binding by $^3H-morphine$ also showed highly significant diurnal change in control and constant dark adapted rats. Statistical analysis by one-way analysis of variance and two-way analysis of variance indicates that the·re are highly significant differences between the diurnal change of ${\beta}-endorphin$ in control and those constant dark adapted and drug treated groups. However diurnal change of Maximum $^3H-morphine$ binding is closely related to the change of ${\beta}-endorphin$ contents. The results are interpreted with regard to the circadian rhythm of beta-endorphin contents, its modification by psychoactive drugs and possible mechanism of diurnal change of opiate receptor in brain.
This study was carried out to seek for a most effective hastening method of seed germination by Pon-Pon treatment and asexual multiplication method by cutting in Zanthozylum piperitum. The results obtained in this study were as follows : 1. Cold moist stratification under the ground accompanied with Pon-Pon treatment was shown 74.3% of seed germination, which was the most effective one among pretreatment methods with significance at 1% level. 2. Height growth was shown continuous growth pattern and the maximum growth was shown during the period 1 July to 31 July, which corresponds 40.3% of total annual growth. 3. Although no noticeable difference among the kind of plant growth regulator in the rooting rate, there was difference among concentrations with significance at 1% level. 4. In the case of number of primary roots and T/R rate of rooted cuttings, treatment of plant growth regulators was significantly superior to control. 5. T/R rate in rooted cuttings was a high negative correlation (r=-0.7164) with the number of primary roots.
This study describes analysis of genetic diversity and spatial genetic structure of Korean poplar (Populus koreana Rehder) in Mt. Odae using I-SSR markers. P. koreana is a deciduous broad-leaved tree species that primarily grows in the alpine valleys of China, Russia and North Korea. In South Korea, P. koreana is found limitedly in Gangwon province. Especially, the population in Mt. Odae is located on the southern limit line, its importance is emphasized from the genetic resource conservation perspective. The Shannon's diversity (I=0.230) and the expected heterozygosity (He=0.151) were relatively low as compared with those of other plant species. Spatial autocorrelation analysis using Tanimoto's distance showed that the genetic patch was founded within 400 m. It is suggested that individual trees for ex situ conservation should be sampled with a minimum distance of 400 m between trees.
Journal of the korean academy of Pediatric Dentistry
/
v.28
no.3
/
pp.403-411
/
2001
The aim of this study was to evaluate the resistance to degradation of four commercial composite resins in an alkaline solution. The brands studied were Unifil(GC, Japan), Palfique(Tokuyama Japan). Definite$Degussa-H\ddot{u}ls$ AG, Germany). Revolution(Kerr, U.S.A.). Preweighed discs of each brand were exposed 0.1N NaOH solution at $60^{\circ}C$. After 14 days they were removed, neutralized with HCl, washed with water and dried. Resistance to degradation was evaluated on the basis of following parameters: (a) mass loss(%) - determined from pre-and post-exposed specimen weights; (b) Si loss(ppm) - obtained from ICP-AE analysis of solution exposed to specimens; and (c) degradation depth$({\mu}m)$ - measured microscopically (SEM) from polished circular sections of exposed specimens. The results were follows: 1. The mass loss of Unifil was 3.21%, it was the highest of materials. But, there was no significant difference among the materials. 2. The degree of degradation layer depth was $107.69\sim47.40{\mu}m$, the sequence of the degree pf degradation layer depth was in descending order by Unifil, Palfique, Revolution, Definite. There was significant difference among the materials except Palfique and Definite. 3. The Si loss of Paltique was 8940.0ppm, it was the highest. There was significant difference among the materials, except Revolution and Definite(p<0.05). 4. The correlation coefficient between mass loss and degradation depth was relatively high(r = 0.06, p<0.05). 5. There was no significant coefficient correlation between Si loss and mass loss, and/or the degree of degradation layer depth and Si loss. 6. When observed with SEM, destruction of bonding is observed between resin matrix and filler. Above results suggested that the hydrolytic degradation is considered as evaluation factor of composite resins.
Nutritional composition and phenolic compounds of raw propolis collected from falseacacia (Robinia pseudoacacia L.) and chestnut tree (Castanea crenata), and their 70% ethanol extracts of propolis (EEP) were analyzed. Propolis had high crude lipid content, but no significant differences in general compositions in terms of collection area and plant origins. Mineral contents varied greatly depending on the plant origins, with falseacacia propolis showing the highest mineral content. Sixteen amino acids were analyzed, among which aspartic acid content was the highest at $328.4{\sim}410.6\;mg%$ and methionine the lowest at $0{\sim}21.1\;mg%$. Extraction yield for EEP was relatively high at $64.2{\sim}81.9%$, and total polyphenol and flavonoid contents were $13.9{\sim}23.7$ and $8.6{\sim}10.8%$, respectively. HPTLC and HPLC analysis on the phenolic compounds revealed the overall chromatographic patterns were almost equal, showing similar polyphenol compositions between the propolis. About 16 peaks were identified by HPLC analysis, among which 6 peaks of p-hydroxy benzoic acid, caffeic acid, ferulic acid, benzoic acid, cinnamic acid, and chrysin were identified.
Nitrogen efficiency for grain yield(E) and its relation to grain yield(Y), harvesting index(HI), percent translocation of nitrogen from straw to grain(T), nitrogen uptake amount(N), concentration in grain (GN%) or straw (SN%) and total dry matter yield (TY) among rice varieties (Oryza sativa, old and new varieties) were investigated at four nitrogen nutritional status (high and low fertilizer levels in high and low fertility fields) by simple correlation analysis. Relation between any two of above parameters or total dry matter yield (TY) and nitrogen efficiency for total dry matter yield (TE) was also investigated. 1. E is significantly and positively correlated with T, Y, HI but negatively with SN%, N, GN% and in negative trend with TY. 2. T is significantly and positively with GN% or Y, but negatively with SN%. 3. TE is significantly and positively correlated with TY but negatively with N. 4. The order of E among varieties showed consistency among different nitrogen nutritional environments. 5. From the above facts it was concluded that high yielding varieties have high nitrogen efficiency due to high percent translocation of nitrogen from straw to grain, subsequent low nitrogen concentration in straw and that translocated nitrogen in grain is greatly diluted with photosynthates. 6. Reported physiological characteristics of newly bred high yielding IR lines are well accordance with their high nitrogen efficiency and rice breeding was a selection on the basis of nitrogen efficiency. 7. It is postulated that high nitrogen efficiency varieties for yields have high nitrogen efficiency for root growth in early stage so that uptake more efficient soil nitrogen in later growth stage.
An, Ul-Jin;Noh, Hong-Seok;Jeong, Tae-Sung;Kim, Shin
Journal of the korean academy of Pediatric Dentistry
/
v.38
no.2
/
pp.119-128
/
2011
In the process of assessing the children with anterior crossbite in early mixed dentition, it has frequently been detected that the stronger the skeletal pattern of the malocclusion is, the more markedly delayed the development and eruption of maxillary teeth are. If the anteroposterior characteristics of craniofacial skeleton has any relationship with dental maturation, the evaluation of dental development and eruption was thought to be able to contribute to early diagnosis of crossbite in children. This study was performed for the purpose of elucidating the relationship between dental maturation of maxillary teeth and some cephalometric values in children with anterior crossbite of maxillary undergrowth type in early mixed dentition. Among the children in Hellman dental age IIA and IIC who attended the Pediatric Dental Clinic of Pusan National University Hospital with orthodontic problems, cases with Class III malocclusion were classified and 50 cases of maxillary undergrowth type and type with normal maxilla respectively were randomly selected and studied as subjects. From their lateral cephalographs and panoramic radiographs, their anteroposterior skeletal features, the dental maturity and eruption rate were obtained of each group and data were analyzed to yield the results as follows: 1. Comparing the maturity of maxillary teeth of both groups, only the first molars of maxillary undergrowth group showed significantly slower development and eruption (p<0.05). 2. There was high correlation between maturation of maxillary 1st molar and chronological age(p<0.05). 3. Among the parameters of anteroposterior relationship of skeletal pattern in maxilla and mandible. Wits was revealed as a useful index to predict both the calcification and eruption rate of the 1st molars whereas SNA was to eruption rate(p<0.05).
Journal of the Korean Institute of Landscape Architecture
/
v.50
no.2
/
pp.23-40
/
2022
Many constructed wetlands have been installed in dam reservoirs nationwide for ecological purification of watershed pollutants, but aging and reduced efficiency are becoming issues. To improve the management of constructed wetlands, an objective evaluation of structural suitability is required. This study evaluated 39 constructed wetlands of 15 dams. First, through fogus group interview(FGI), survey analysis, and analytic hierarchy process(AHP), eight evaluation items in the physical and vegetative aspects were selected and the evaluation criteria applied with weights were prepared. Second, as a result of the structural suitability evaluation, the average score of the overall constructed wetlands was 80.8, with 10 sites rated as 'good grade(91~100)', 22 sites rated as 'normal grade(71~90)' and 7 sites rated as 'poor grade(70 or less)'. The average score of physical structure evaluation was 52.6, with 14 sites rated as 'good', 21 sites as 'normal' and 4 sites as 'poor'. The suitability of location was good level in most constructed wetlands, but the water supply system, depth of water, ratio of length-to-width, and slope of flow channel were evaluated as 'normal' or less in constructed wetlands of 50% or more. Therefore, it was found that overall improvement was necessary for stable flow supply and flow improvement in the constructed wetland. The average score of vegetative structure evaluation was 28.2, and about 84% of them were identified as 'normal' or lower. As a result of analyzing the Spearman's correlation coefficient between the physical structure evaluation score and the vegetation structure evaluation score, there was a significant correlation(r = 0.728, p < 0.001), and it was found that each evaluation factor also influences each other. As a result of the case review of 6 constructed wetlands, the appropriateness of the evaluation results was confirmed, and it was found that the location, flow rate supply, and type of wetland had a great influence on the efficiency and operation of the wetland. Through this study, it will be possible to derive structural weaknesses of constructed wetlands in dam reservoirs as a nature-based solution, to prepare types and practical alternatives for improved management of each constructed wetland in the future, and to contribute to enhancing various environmental functions.
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