• KSBB Journal
• /
• v.20 no.5 s.94
• /
• pp.350-354
• /
• 2005

In order to maximize hydrogen production by Enterobacter cloacae YJ-1, anaerobic hydrogen producing bacteria, the medium composition was optimized. Glucose was better than other carbon sources in hydrogen production and its production was 975.4 mL/L at $2\%$ (w/v) for 48 h. Organic nitrogen sources were more effective than inorganic nitrogen sources and also yeast extract among organic nitrogens was the most effective in hydrogen production. Among metal ions, $Na_2MoO_4$ was most effective, and its production was 1753.3mL/L at $0.04\%$ (w/v). Addition of amino acid was very effective with compare to another components of medium, and cystein was most effective among them. Under the optimum medium obtained in batch culture, semi-batch culture in order to produce continuous hydrogen was run. The highest hydrogen production was earned at $3\%$(w/v) of glucose and the amount was 2215.4 mL/L.

• Analytical Science and Technology
• /
• v.13 no.4
• /
• pp.466-473
• /
• 2000

The complex formation constants (${\beta}_{MLn}$) of potassium and various sodium-selective neutral carriers in solvent polymeric membranes have been determined using solvent polymeric membrane-based optodes and ion-selective electrodes (ISEs). Two different types of PVC-based membranes containing the H^+selective chromoionophore (ETH 5294) with and without a sodium ionophore (4-tert-bntylcalix[4]arenetetraacetic acid tetraethyl ester, ETH 2120, bis[(12-crown-4)methyl] dodecylmethylmalonate or monensin methyl ester) were prepared and their optical responses to either the changes in alkali metal cation (e.g., sodium and potassium) concentrations at a fixed pH (0.05 M Tris-HCl, pH 7.2) or varying pH at a fixed alkali metal cation concentration (0.1 M) were measured. The same type of membranes were also mounted in conventional electrode body and their potentiometric responses to varying pH at a fixed alkali metal cation concentration (0.1 M) were measured. The complex formation constants of the ligand could be calculated from the calibration plots of the relative absorbance vs. the activity ratios of cation and proton ($a_{M^+}/a_{H^+}$) and of the emf vs. pH. It was confirmed that the ratio values of the complex formation constants for the primary and interfering ions are closely related to the experimental selectivity coefficients of ISEs.

• Journal of the Korean Applied Science and Technology
• /
• v.35 no.4
• /
• pp.1433-1441
• /
• 2018

The development of acidosis during intense exercise has traditionally been explained by the increased production of lactic acid which causes the release of a proton and the formation of the acid salt sodium lactate. Through this explanation, when the rate of lactate production is high enough to exceed cellular proton buffering capacity, cellular pH is decreased. This biochemical process has been termed lactic acidosis. This belief has been an interpretation that lactate production causes acidosis and fatigue during intense exercise. However, this review provides clear evidence that there is no biochemical support for lactate production causing acidosis and fatigue. Metabolic acidosis is caused by an increased reliance on nonmitochondrial ATP turnover. Lactate production is essential for muscle to produce cytosolic $NAD^+$ to support continued ATP regeneration from glycolysis. In addition, Lactate production consumes protons. Although lactate accumulation can be a good indirect indicator for decreased cellular and blood pH, that is not direct causing acidosis.

• Journal of Life Science
• /
• v.27 no.7
• /
• pp.805-811
• /
• 2017

In the previous paper, we isolated a bacterium that can hydrolyze various organic materials from soybean paste, including cellulose, lipids, starch, and protein. The activity and chemical properties of the crude enzymes produced by the isolate Bacillus subtilis CK-2 were further investigated. Cellulase showed the highest activity at pH 5.0 and $55^{\circ}C$. The stability of cellulase was maintained within the ranges of pH 5.0~10.0 and $20{\sim}50^{\circ}C$. Cellulolytic enzymes were activated by a $Co^{2+}$ ion, demonstrating the highest activity at a 0.45%(w/v) concentration of $Co^{2+}$. The optimal conditions for amylase were pH 5.0 and $50^{\circ}C$. The activity of amylase was stable within the ranges of pH 4.0~5.0 and $20{\sim}50^{\circ}C$. The $Co^{2+}$ ion was also necessary for amylase activity, which was the highest at a 0.2%(w/v) concentration of $Co^{2+}$. The optimal pH and temperature conditions of protease were pH 8.0 and $50^{\circ}C$. The activity of protease was stable within the ranges of pH 7.0~8.5 and $20{\sim}50^{\circ}C$. Protease activity was catalyzed by $Mn^{2+}$, which was the highest at a 0.125%(w/v) concentration of $Mn^{2+}$. The isolate B. subtilis CK-2 demonstrated a high activity of autolysin. Based on these results, we identified and suggested the optimal pH, temperature, and metal ion concentration in the use of the hydrolytic enzymes of B. subtilis CK-2 for industrial purposes.

• Geophysics and Geophysical Exploration
• /
• v.12 no.1
• /
• pp.163-171
• /
• 2009

Subcritical crack growth is one of the main causes of time-dependent fracturing in rock. In the present study, we investigated subcritical crack growth in rock in distilled water (pH = 5.7) and in an aqueous solution of sodium hydroxide (NaOHaq, pH = 12), comparing the results to those in air. We also investigated the effect of the pH in an aqueous environment. We used andesite and granite for all our tests. We determined the relationship between the crack velocity and the stress intensity factor using the double-torsion test under conditions of controlled temperature. We showed that crack velocities in water were higher than those in air, in agreement with other research results indicating that crack velocity increases in water. When we compared our results for NaOHaq with those for water, however, we found that the crack velocity at the same stress intensity factor did not change even though the pH of the surrounding environment was different. This result does not agree with the accepted understanding that hydroxide ions accelerate subcritical crack growth in rocks. We concluded that the pH at the crack tip influences subcritical crack growth, and not the bulk pH, which has little effect.

• Journal of the Korean Society of Visualization
• /
• v.8 no.4
• /
• pp.38-42
• /
• 2010

Ion concentration polarization is an electrokinetic phenomenon which occurs in membrane systems, such as in an electrodialysis and fuel-cell system. But the phenomenon is not fully understood because hydrodynamics, electrokinetics and electrochemistry are coupled with each other. Here, we report that there occurs a change of pH value of buffer solution in concentration polarization phenomenon. To visualize the change of pH, the litmus solution which is one of the pH indicators was used. It is conjectured that the pH of solution changes because hydrogen ions were concentrated in cathodic side and hydroxide ions were concentrated in anodic side. We anticipate that this work may contribute to the fundamental understanding on the ion concentration polarization phenomenon.

• Journal of Korean Society of Environmental Engineers
• /
• v.29 no.1
• /
• pp.68-73
• /
• 2007

In this study, we investigated the decomposition of highly concentrated hydrogen peroxide in the range of 1.04-2.55 M by transition metal ion catalysts such as $Fe^{2+}$ and $Cu^{2+}$. The effect of metal ion concentration on the decomposition of hydrogen peroxide was examined experimentally, and the decomposition rate constants were determined by combining the experimental data with a theoretical approach. The rate of the decomposition of hydrogen peroxide was found to be first order with respect to its concentration. The decomposition rate constant was able to be treated as a linear function of the initial metal ion concentration. The validity of the decomposition rate constants determined was verified by good agreements between the calculated and experimental results.

• Analytical Science and Technology
• /
• v.14 no.5
• /
• pp.416-421
• /
• 2001

The optimization of the reductant and acid concentration for stannane($SnH_4$) generation was investigated by using a continuous flow hydride generator combined with an inductively coupled plasma-atomic emission spectrometer. Several different prereductants were studied to remove the interfering effect of fluoride ion on the hydride generating of tin. The optimum acid concentration was 0.5-1.0 M for the 1-2% $NaBH_4$ and 1.0 M NaOH and the interfering effect of fluoride ion was minimized using boric acid and L-cysteine mixed solution as a prereductant. The reconveries of tin at 20 ng/mL level in the solution containing fluoride ion were 100~108 %.

• Korean Chemical Engineering Research
• /
• v.57 no.3
• /
• pp.438-443
• /
• 2019

A new measurement method is suggested to quantify the phosphate poisoning of cathodic Pt catalyst for HT-PEMFC. To do that, hydrogen peroxide was used as an indicator to reduce the error which has been occurred in conventional electrochemical measurement such as CV or ORR RDE with high concentration of phosphate ions. As a result, the current density induced from the reaction of hydrogen peroxide decomposition increased proportionally to the concentration of phosphate ion while the conventional methods show has a significant error with high concentration of phosphate ion. Thus, it is confirmed that the suggested way is superior to the conventional measurement method for the quantification of phosphate ion poisoning in an atmosphere similar to the actual operation condition of HT-PEMFC.

• Korean Journal of Animal Reproduction
• /
• v.25 no.2
• /
• pp.113-118
• /
• 2001

The objective of this study was to investigate the effects of $\alpha$-tocopherol and selenium on the boar semen characteristics. Semen volume and pH values were not different among treatments. However sperm concerntration, total number of sperm and sperm mortility were significantly(P＜0.05) increased comparing to the control group and sperm abnormality was significantly(P＜0.05) decreased comparing to the control group. Also, sperm mortility by storage day was significantly(P＜0.05) increased comparing to the control group. The results from this experiment indicate that dietary $\alpha$-tocopherol and selenium can affect boar semen characteristics.