• Proceedings of the Korean Environmental Sciences Society Conference
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• 2005.05a
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• pp.255-256
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• 2005

비스페놀 A, 노닐페놀 및 아트라진의 농도에 따른 생존율을 조사한 결과 같은 농도인 경우 노닐페놀에 노출한 실험군에서 상대적으로 낮은 생존율을 보였고, 비스페놀 A와 노닐페놀을 혼합한 실험군에 홍삼을 첨가한 경우 생존율은 상대적으로 높게 나타남을 알 수 있었다. 따라서 홍삼이 내분비계 장애물질의 독성을 부분적으로나마 억제하는 효과가 있는 것으로 사료된다.

• THE HEALTH and ORGANIC FARMING
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• no.209
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• pp.1-12
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• 2004

제26회 한국유기농업대회 성황리 개최/우리모두 유기농업으로 무장한 한마리의 송사리가 되자!/국제 CODEX에 준한 한국 유기농업의 실천사례/제초제가 필요없는 쌀겨농법/유기농업의 기초/무 엇을 먹을까? 어떻게 먹 을까?/유기농업대회 화보/제6회 친환경농산물 품평회/친환경유기농산물 소비·유통의 활성화 방안/21C 지속가능한 사회를 향한 국가와 기업의 새 경영 패러다임/순환농업의 지역별 유형/국제 기준에 의한 유기농산물 생산기술/오이 검은별 무늬병 방제/딸기 흰가루병의 발생생태/가축분뇨 퇴비 품질기준 및 퇴비화 악취 저감기법/친환경농업에서의 규소의 적용성

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.173-174
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• 2000

Yamamoto (1953)가 송사리, Oryzias latipes를 대상으로 성전환에 관한 일련의 연구결과를 발표한 이후 어류를 대상으로 한 성전환 연구보고가 많이 이루어지고 있고(Clemens and Inslee, 1968; Jalabert et al., 1974; Yamazaki; 1976; Goetz et al., 1979; Donaldson and Hunter, 1982; Tanaka, 1988), 현재 어류의 성제어 기술은 연어, 송어, 넙치 등에서 산업적 응용이 가능한 단계에 이르고 있다. (중략)

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.149-150
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• 2000

어류의 성결정 양상은 크게 유전적 성결정, 생리적 성결정 그리고 환경적 성결정이 알려지고 있다(Yamazaki, 1983; Abucay et al., 1999). 그러나, 최근에 들어 PCB(Polychlorinated Biphenyls), 노닐페놀, 비스페놀 및 다이옥신 등 내분비장애물질(Endocrine Disrupter Chemicals)에 노출되었을 때에도 어류의 성결정에 영향을 미친다는 연구가 보고되고 있다(Gray, et al., 1997). 어류의 생리적 성전환을 유도한 연구는 Yamamto(1953)가 송사리, Oryzias latipes를 대상으로 생리적인 성전환을 유도한 이후 이에 대한 많은 연구가 보고되고 있다. (중략)

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.5
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• pp.462-473
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• 2010

Inductions of hybrids and reciprocal hybrids between Oryzias dancena and O. javanicus (ODJ and OJD) were conducted and backcross hybrids between female O. dancena and male ODJ were also produced for biological and cytogenetic analysis. Embryonic development of ODJ and OJD were compared with those of their parents. Developmental time was fastest in O. dancena and ODJ, followed by O. javanicus and OJD. Oryzias dancena hatched 11 days (d) after fertilization, ODJ at 13 d, O. javanicus at 14 d and OJD at 15 d. The abnormality of external morphology rate in ODJ was 10.6%; however, OJD showed a high degree of abnormality, over 90%. The proportion of males was 90.0% and 31.3% for ODJ and OJD, respectively. Cytogenetic analysis was conducted to obtain basic information for genetic identification of O. dancena, O. javanicus and their hybrids. The karyotypes of all experimental groups showed 2n=48 chromosomes and the fundamental number (FN) was 48. The first pair carried secondary constrictions near the centromeric regions. Erythrocyte area and volume were $9.8\;{\pm}\;0.5\;{\mu}m^2$ and $18.2\;{\pm}\;1.0\;{\mu}m^3$, respectively, for O. dancena, $8.3\;{\pm}\;0.5\;{\mu}m^2$ and $15.8\;{\pm}\;1.5\;{\mu}m^3$ in O. javanicus, and $18.3\;{\pm}\;0.5\;{\mu}m^2$ and $15.7\;{\pm}\;1.3\;{\mu}m^3$ in ODJ. Erythrocyte area and volume in ODJ were similar to those of O. javanicus. In backcross hybrids between female O. dancena and male ODJ, all embryos failed to develop and died in the late gastrula stage.

• Korean Journal of Ichthyology
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• v.18 no.1
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• pp.12-19
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• 2006

To examine the relationship of geological distribution and phylogenetic tree of O. latipes in the Far East, we analyzed cytochrome b (cyt b) gene in the mitochondrial genome. In this study we employed the entire sequence of cyt b of 53 samples collected from nine Korean locations and 117 cyt b data retrieved from the GenBank. From 170 Oryzias latipes cyt b sequence data, 142 different haplotypes were identified and phylogenetic relationship was reconstructed based on the dataset. According to the phylogeny, haplotypes were divided into three major haplogroups A, B and C, and their relationships were well correlated to their distributional patterns. Haplogroup A which is widely distribute in the southern part of Korea is separated in the geographical distribution from the haplogroup B which is found from China to the western part of Korea. Haplogroup C is only found in Japan.

• Environmental Analysis Health and Toxicology
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• v.22 no.3
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• pp.203-209
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• 2007

생활하수, 공장폐수, 농경유출수에 의해 수생태계로 유입된 다양한 화학물질들은 수서곤충이나 어류와 같은 수생생물에게 나쁜 영향을 주곤 한다. 비스페놀A와 노닐페놀을 포함하는 많은 화학물질들이 내분비계 장애물질(EDCs)로 의심되고 있고, 그들은 환경속에서 서로 다른 혼합형태로 공존하기도 한다. 따라서 비스페놀A와 노닐페놀의 혼합물이 독성과 생식학적 반응에 미치는 영향을 살펴보기 위해 일본산 송사리의 수정란 치사율, 부화율 및 부화시간, 치어의 성장율 및 비텔로제닌 농도 등이 측정되었다. 수정된 지 24시간 이내의 수정란을 대조군, 양성대조군($17{\beta}-estradiol$), 그리고 서로 다른 농도의 비스페놀A와 노닐페놀의 혼합물에 부화 후 60일까지 유수식 조건하에 노출시켰다. 수정란${\sim}$치자어 단계에서는 대조군과 비교하여 실험군의 치사율 및 부화율, 부화시간에 차이가 나타나지 않았으며, 부화 후 60일간의 노출 후 성장(길이, 무게)에 있어서도 비록 양성대조군에서 낮은 성장상태를 보였지만 다른 혼합물의 실험군들과는 차이를 보이지 않았다. 한편 체내 비텔로제닌 농도는 혼합물의 농도증가에 따라 증가하였으며 수컷의 경우 최저농도의 혼합물(Treatment A)을 제외한 실험군에서 농도증가에 따라 증가하였다. 반면 양성대조군의 경우 수컷이 발견되지 않았고 암컷 체내의 비텔로제닌 농도는 최고농도의 혼합물(Treatment D) 실험군과 비슷한 경향을 보였다. 위 실험을 통해 각각의 내분비계 장애물질이 개별적으론 생식발달 및 비텔로제닌 유도에 무영향농도(NOEC)라 하더라도 혼합된 경우 영향이 나타날 수 있다는 것을 보여주었으며, 이는 수환경 내 다양한 화학물들의 혼합효과(combination effect)가 생태위해성평가를 좀더 면밀하게 하기 위해서 주의 깊게 고려되어야 한다고 제안한다.

• Environmental Analysis Health and Toxicology
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• v.15 no.4
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• pp.131-137
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• 2000

The release of hazardous waste materials into the environment poses serious risks in humans and ecosystems. The risk assessment of environmental pollutants including hazardous chemicals requires a comprehensive measurement of hazard and exposure of the chemicals that can be achieved by toxicity evaluation using a biological system such as biomarkers. In this report we have tried to develop a biomarker used to elucidate a molecular basis of, and to monitor abnormal behaviors caused by diazinon in Japanese medaka (Oryzias latipes) as a model organism. First, an attempt was made to clone tyrosine hydroxylase gene from Japanese medaka that would be a candidate for a biomarker for neuronal modulations and behaviors. For monitoring experiments at behavioral and molecular biological levels, the fish were treated under different sublethal conditions of diazinon and their behavioral responses were observed . In this study we have successfully cloned a partial TH gene from the medaka fish through PCR screening of an ovary cDNA library. DNA sequencing analysis revealed that the amplified fragment was 327 bp encoding 109 amino acids. Comparing the DNA sequence of medaka TH with other species, TH gene revealed the DNA sequence was completely identical to that of rat TH. In the RT-PCR, 330 Up of mRNA was consistently amplified in all the treated samples including control There were no significant differences in the TH expression level regardless of treating concentrations (1∼5,000 ppb) and time (0∼48 hr) The reason appeared to be that RT-PCR was not performed using through a quantitative analysis normalized against an actin gene expression. Organ or tissue - specific detection of TH activity and mRNA as biomarkers will be a useful monitoring tool for neurobehavioral changes in fish influenced by toxic chemicals. Furthermore, quantitative analysis of locomotive patterns and its correlation with the neurochemical and molecular data would be highly useful in measuring toxicity and hazard ofvarious environmental pollutants.

• Journal of fish pathology
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• v.24 no.2
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• pp.131-139
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• 2011

Bisphenol A (BPA) is mainly used in the production of epoxy resins and polycarbonate plastics, which is a known endocrine disruptor and acutely toxic to aquatic organisms. In this study, estrogenic effect of BPA was investigated on hybrid between Oryzias dancena and O. javanicus (ODJ). ODJ were exposed to BPA of various concentrations (eg. 2.5 mg/L, 5.0 mg/L and 10.0 mg/L) for 56 days. The growth rate, abnormality and the ratio of female and male were observed in test group and control group. As a result, the growth was $14.7{\pm}2.0$ mm in total length (TL) in 2.5 mg/L, $13.7{\pm}2.5$ mm in 5.0 mg/L, $12.8{\pm}2.5$ mm in 10.0 mg/L in test group while it was $18.0{\pm}1.2$ mm in TL in control group which was not treated with bisphenol A. The result showed that the growth decreased as the concentration of BPA increased. The abnormality rate was 13.6% in control group, 65.4% in 2.5 mg/L, 81.3% in 5.0 mg/L and 98.1 % in 10.0 mg/L which showed increase in abnormality as an increase of BPA concentration. As a result of analyzing ratio of sex in the test group and control group, 6.0% was examined to be interspecific in controls, 76.9% in 2.5 mg/L and 100.0% in 5.0 mg/L and 10.0 mg/L. In conclusion, these results suggest that BPA has estrogenic effect on ODJ.

• Korean Journal of Environmental Biology
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• v.25 no.1
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• pp.1-7
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• 2007

Medaka(Oryzias latipes) and earthworm(Eisenia fetida) toxicity tests were carried out and ecological risk assessment in water and soil was performed with national monitoring data. NOEC of alachlor was $100\;{\mu}g\;L^{-1}$ in the medaka early life-stage test. Embryonic development, hatchability and time to hatching of medaka eggs were affected by this chemical. The $LC_{50}$ and NOEC of alachlor were $94.1\;mg\;kg^{-1}\;and\;55.0\;mg\;kg^{-1}$, respectively, in the earthworm acute toxicity test. The environmental monitoring has been carrying out by NIER since 1999. Exposure levels of alachlor in water and soil were $ND{\sim}0.54\;{\mu}g\;L^{-1}\;and\;ND{\sim}0.9\;{\mu}g\;kg^{-1}$, respectively, in national monitoring data which had been performed from 2000 to 2004. The measured water and soil exposure levels were applied to evaluate the environmental risk assessment. The PNEC of alachlor in water and in soil were determined as $1\;{\mu}g\;L^{-1}\;and\;55.0\;{\mu}g\;kg^{-1}$, respectively using the safety factors which were suggested in EU and OECD. The HQs (PEC/PNEC) were determined to be below 1 for both water and soil when the maximum exposure levels ($0.54\;{\mu}g\; L^{-1}$ in water and $0.9\;{\mu}g\;kg^{-1}$ in soil) were applied. Conclusively, our study indicated that there was not significant ecological risk of alachlor in water and soil of our monitoring sites.